Objective In kinesin-3,the neck coil correlates with the following segments to form an extended neck that contains a characteristic hinge diverse from a proline in KIF13B to a long flexible linker in KIF1A.The functio...Objective In kinesin-3,the neck coil correlates with the following segments to form an extended neck that contains a characteristic hinge diverse from a proline in KIF13B to a long flexible linker in KIF1A.The function of this neck hinge for controlling processive movement,however,remains unclear.Methods We made a series of modifications to the neck hinges of KIF13B and KIF1A and tested their movement using a single-molecule motility assay.Results In KIF13B,the insertion of flexible residues before or after the proline differentially impacts the processivity or velocity,while the removal of this proline increases the both.In KIF1A,the deletion of entire flexible neck hinge merely enhances the processivity.The engineering of these hinge-truncated necks of kinesin-3 into kinesin-1 similarly boosts the processive movement of kinesin-1.Conclusion The neck hinge in kinesin-3 controls its processive movement and proper modifications tune the motor motility,which provides a novel strategy to reshape the processive movement of kinesin motors.展开更多
A non-equilibrium statistical method is used to study the collective characteristics of myosin II motors in a sarcomere during its contraction. By means of Fokker-Planck equation of molecular motors, we present a dyna...A non-equilibrium statistical method is used to study the collective characteristics of myosin II motors in a sarcomere during its contraction. By means of Fokker-Planck equation of molecular motors, we present a dynamic mechanical model for the sarcomere in skeletal muscle. This model has been solved with a numerical algorithm based on experimental chemical transition rates. The influences of ATP concentration and load on probability density, contraction velocity and maximum active force are discussed respectively. It is shown that contraction velocity and maximum isometric active force increase with the increasing ATP concentration and become constant when the ATP concentration reaches equilibrium saturation. Contraction velocity reduces gradually as the load force increases. We also find that active force begins to increase then decrease with the increasing length of sarcomere, and has a maximum value at the optimal length that all myosin motors can attach to actin filament. Our results are in good agreement with the Hill muscle model.展开更多
文摘Objective In kinesin-3,the neck coil correlates with the following segments to form an extended neck that contains a characteristic hinge diverse from a proline in KIF13B to a long flexible linker in KIF1A.The function of this neck hinge for controlling processive movement,however,remains unclear.Methods We made a series of modifications to the neck hinges of KIF13B and KIF1A and tested their movement using a single-molecule motility assay.Results In KIF13B,the insertion of flexible residues before or after the proline differentially impacts the processivity or velocity,while the removal of this proline increases the both.In KIF1A,the deletion of entire flexible neck hinge merely enhances the processivity.The engineering of these hinge-truncated necks of kinesin-3 into kinesin-1 similarly boosts the processive movement of kinesin-1.Conclusion The neck hinge in kinesin-3 controls its processive movement and proper modifications tune the motor motility,which provides a novel strategy to reshape the processive movement of kinesin motors.
基金supported by the National Natural Science Foundation of China (Grant No. 61075101/60643002)the Research Fund of State Key Laboratory of MSV, China (Grant No. MSV-2010-1)+2 种基金the National High-Tech Research and Development Program of China (Grant No. 2006AA04Z240)the Shanghai Dawn Program (Grant No. 07SG14)the Medical and Technology Intercrossing Research Foundation of Shanghai Jiao Tong University (Grant No. YG2010ZD101)
文摘A non-equilibrium statistical method is used to study the collective characteristics of myosin II motors in a sarcomere during its contraction. By means of Fokker-Planck equation of molecular motors, we present a dynamic mechanical model for the sarcomere in skeletal muscle. This model has been solved with a numerical algorithm based on experimental chemical transition rates. The influences of ATP concentration and load on probability density, contraction velocity and maximum active force are discussed respectively. It is shown that contraction velocity and maximum isometric active force increase with the increasing ATP concentration and become constant when the ATP concentration reaches equilibrium saturation. Contraction velocity reduces gradually as the load force increases. We also find that active force begins to increase then decrease with the increasing length of sarcomere, and has a maximum value at the optimal length that all myosin motors can attach to actin filament. Our results are in good agreement with the Hill muscle model.
