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奶牛子宫复旧期宫腔内微生物区系研究——常见革兰氏阳性菌的分属鉴定
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作者 王立群 何剑斌 +5 位作者 田文儒 赵凯 范友胜 王广君 王洪诚 李贵全 《中国奶牛》 1999年第5期15-16,共2页
从5 头产后13 ~25 天的正常奶牛子宫内采集并分离到纯种菌20 株,其中革兰氏阳性菌15 株,革兰氏阴性菌5 株。对15 株革兰氏阳性菌进行了简易分属鉴定,其属别为:微球菌属( Micrococcus) 、链球菌属( ... 从5 头产后13 ~25 天的正常奶牛子宫内采集并分离到纯种菌20 株,其中革兰氏阳性菌15 株,革兰氏阴性菌5 株。对15 株革兰氏阳性菌进行了简易分属鉴定,其属别为:微球菌属( Micrococcus) 、链球菌属( Streptococcus) 、葡萄球菌属( Staphylococcus) 、气球菌属( Aercoccus) 、片球菌属( Pediococous) 及芽孢杆菌属( Baciccus) 。5 株革兰氏阴性菌属于大肠菌群的成员。 展开更多
关键词 奶牛 子宫复旧 革兰氏阳性菌 分属鉴定
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Taxonomic note on two Lyristes cicadas(Hemiptera:Cicadidae) described by E. Schmidt from China
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作者 王旭 林正美 魏琮 《Entomotaxonomia》 CSCD 2015年第3期197-200,共4页
The identity of two Lyristes species described by Schmidt is readdressed based on examination of the type materials. Lyristes wui Schmidt, 1932 is recognized to be a junior synonym of Salvazana mirabilis Distant, 1913... The identity of two Lyristes species described by Schmidt is readdressed based on examination of the type materials. Lyristes wui Schmidt, 1932 is recognized to be a junior synonym of Salvazana mirabilis Distant, 1913, and Lyristes altaiensis Schmidt, 1932 is a junior synonym of a North American species, Neotibicen resh (Haldeman, 1852). Lyristes altaiensis may have been erroneously recorded from China most possibly due to the mislabeling of the holotype. 展开更多
关键词 new synonym Salvazana Tibicen Neotibicen IDENTITY distribution
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Recent Advances in Classification and Molecular Biology of Bursaphelenchus Species
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作者 孙柯迪 郑炜 《Agricultural Science & Technology》 CAS 2014年第12期2167-2170,2174,共5页
This paper mainly summarizes recent advances in the classification of Bursaphelenchus species, introduces several common methods of molecular biologi- cal detection and identification methods, and analyzes the advanta... This paper mainly summarizes recent advances in the classification of Bursaphelenchus species, introduces several common methods of molecular biologi- cal detection and identification methods, and analyzes the advantages and disad- vantages of these methods. 展开更多
关键词 BURSAPHELENCHUS Molecular biology IDENTIFICATION
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Isolation and Characterization of a Thermophilic Oil-Degrading Bacterial Consortium 被引量:3
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作者 Gu Guizhou Li Zheng +1 位作者 Zhao Dongfeng Zhao Chaocheng 《China Petroleum Processing & Petrochemical Technology》 SCIE CAS 2013年第2期82-90,共9页
In this study, a thermophilic oil-degrading bacterial consortium KO8-2 growing within the temperature range of 45--65℃ (with 55℃ being the optimum temperature) was isolated from oil-contaminated soil of Karamay in... In this study, a thermophilic oil-degrading bacterial consortium KO8-2 growing within the temperature range of 45--65℃ (with 55℃ being the optimum temperature) was isolated from oil-contaminated soil of Karamay in Xinjiang, China. Denaturing gradient gel electrophoresis (DGGE) showed that there were nine strains included in KO8-2, which originated from the genera of Bacillus, Geobacillus and Clostridium. They all belonged to thermophilic bacteria, and had been previously proved as degraders of at least one petroleum fraction. The crude oil degraded by KO8-2 was analyzed by infrared spectrophotometry, hydrocarbon group type analysis and gas chromatography. The results indicated that the bacterial consortium KO8-2 was able to utilize 64.33% of saturates, 27.06% of aromatics, 13.24% of resins and the oil removal efficiency reached up to 58.73% at 55 ~C when the oil concentration was 10 g/L. Detailed analysis showed that KO8-2 was able to utilize the hydrocarbon components before C19, and the n-alkanes ranging from C20--C33 were signifi- cantly degraded. The ratios of nC17/Pr and nC18/Ph were 3.12 and 3.87, respectively, before degradation, whereas after degradation the ratios reduced to 0.21 and 0.38, respectively. Compared with the control sample, the oil removal efficiency in KO8-2 composting reactor reached 50.12% after a degradation duration of 60 days. 展开更多
关键词 thermophilic bacteria bacterial consortium oil-contaminated soil BIODEGRADATION DGGE
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Isolation and Identification of Aspergillus spp. During One Year in the Hospitals
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作者 Iskender Karalti Gunay Tulay Colakoglu 《Journal of Life Sciences》 2012年第11期1220-1224,共5页
The aim of the present study was monitoring ofAspergillus spp. in six different hospitals in |stanbul. The authors know that disease like aspergillosis illness caused by Aspergillus spp. is very dangerous for people... The aim of the present study was monitoring ofAspergillus spp. in six different hospitals in |stanbul. The authors know that disease like aspergillosis illness caused by Aspergillus spp. is very dangerous for people's health. Therefore, the present work has been performed to evaluate the hospitals' environment. Petri-plate method has been performed for isolation. Samples were taken at six different hospitals and various locations of each. 13 different Aspergillus species and 141 Aspergillus colonies have been isolated in totally. Maximally isolated species are: Aspergillus niger (29.1%). A. nidulans (21.3%), A. candidus (12.8%), A. ochraceus (9.2%), and A. versicolor (7.8%). A. fumigates, A. flavus are the most pathogen species in human which have been isolated minimally. 展开更多
关键词 ASPERGILLUS hospital air Istanbul.
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Identification of Essential Oil Composition of Four Picea Mill, (Pinaceae) Species from Canada
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作者 Alpaslan Kocak Omer Kilic 《Journal of Agricultural Science and Technology(B)》 2014年第3期209-214,共6页
The aim of this study was to determine the volatile composition of essential oil of four Picea Mill. species (Piceapungens Engelm., Picea mariana (Mill.) Britton, Picea glauca (Moench) Voss., Picea rubens Sarg.)... The aim of this study was to determine the volatile composition of essential oil of four Picea Mill. species (Piceapungens Engelm., Picea mariana (Mill.) Britton, Picea glauca (Moench) Voss., Picea rubens Sarg.) needles. The volatile components extracted from these four species needles were analyzed by using headspace solid phase microextraction (HS-SPME)/gas chromatography-mass spectrum (GC-MS) and 31, 34, 27 and 24 compounds were identified representing 91.77%, 92.70% 92.38% and 94.06% of the total oil, respectively. The major constituents were found to be bornylacetate (29.40%), camphor (26.43%), 13-myrcene (7.47%) and camphene (7.01%) in P. pungens; camphene (22.03%), bornylacetate (21.64%), α-pinene (16.62%) and borneol (7.79%) in P. mariana; bornylacetate (31.25%), limonene (17.27%), α-pinene (15.85%); camphene (13.65%) in P. glauca and borneol (12.38%), α-pinene (10.36%), germacrene D (9.86%) and δ-cadinene (8.25%) in P. rubens. This study sought to detecte new phytochemical data on the Picea genus to help chemotaxonomy and usable of studied species. 展开更多
关键词 PICEA essential oil PINACEAE CANADA HS-SPME/GC-MS.
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Metal cation exchanged silica gel for identification of cationic and nonionic surfactants with preliminary separation by thin layer chromatography
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作者 Mohammacl Ali Khatoon Shumaila 《Journal of Chemistry and Chemical Engineering》 2009年第6期44-48,共5页
Silica gel impregnated with 1% aqueous solutions of different metal cations (Li^+, Mg^2+, Zn^2+, Cu^2+, Co^2+, Ni^2+, Ba^2+and Th^4+) has been used for the analysis of nonionic and cationic surfaetants using... Silica gel impregnated with 1% aqueous solutions of different metal cations (Li^+, Mg^2+, Zn^2+, Cu^2+, Co^2+, Ni^2+, Ba^2+and Th^4+) has been used for the analysis of nonionic and cationic surfaetants using simple aqueous acetone as mobile phase system. Co^2+ was found the most suitable impregnant for the mutual separation of nonionic surfactants (Brij-35 and Brij-57) and cationic from nonionic surfactants (tetmdecyltrimethylammonium bromide and Cween-20). Zinc sulphate impregnation (Zn^2+-silica gel) shows identical chromatographic behavior and these layers are useful to separate nonionic surfactant (Brij-35) from cationic surfaetant (cetylpyridinium chloride). The mutual separation of B J-35 and B J-57 is not influenced by the presence of optical brightener in the sample. 展开更多
关键词 TLC metal cations silica gel SURFACTANTS
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Chemical constituents from the leaves of Premna microphylla Turcz 被引量:12
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作者 胡正喜 薛永波 +3 位作者 姚广民 罗增伟 王燕燕 张勇慧 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2013年第5期431-434,共4页
Phytochemical investigation of the leaves of Premna microphylla Turcz led to the isolation of 13 known compounds. Based on spectroscopic and chemical evidences, their structures were identified as diosmetin (1), blu... Phytochemical investigation of the leaves of Premna microphylla Turcz led to the isolation of 13 known compounds. Based on spectroscopic and chemical evidences, their structures were identified as diosmetin (1), blumenol A (2), (3S,5R,6S,7E,9R)-5,6-epoxy-3,9-dihydroxy-7-megastigmene (3), 3β-hydroxy-5a,6a-epoxy-γ-megastigmen-9-one (4), ixerol B (5), (-)-dehydrovomifoliol (6), 3S,5R-dihydroxy-6S,7-megastigmadien-9-one (7), loliotide (8), (+)-dehydrololiolide (9), (+)-medioresinol (10), 4-oxopinoresinol (11), tormentic acid (12), and indole-3-carboxylic acid (13). Compounds 2-13 described above were isolated from this genus for the first time. 展开更多
关键词 VERBENACEAE Premna Premna microphylla Turcz Chemical constituents Structural identification
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