Aim To analyse the static temperature field ofthe solid rubber tire(SRT).Methods The mechanical and thermal FE models were developed and analyzed respectively with the FE software ANSYS.Results The maximum temperature...Aim To analyse the static temperature field ofthe solid rubber tire(SRT).Methods The mechanical and thermal FE models were developed and analyzed respectively with the FE software ANSYS.Results The maximum temperature becomes higher with the higher with the higher velocity of tire and scales down slightly with the higher convection coefficients.The mixed models are reasonable.Conclusion The study on static temperature field is important and reasonable.It gives the fundament for life analysis of SRT.展开更多
First,the analytical hierarchy process(AHP),which stands for the subjective weighting method,and the entropy method,which stands for the objective weighting method,are chosen to calculate the index weights of the cont...First,the analytical hierarchy process(AHP),which stands for the subjective weighting method,and the entropy method,which stands for the objective weighting method,are chosen to calculate the index weights of the contract risks of third party logistics(TPL),respectively.Then,they can determine the combination weights using the combination weighting method.Second,using the combination weights,the contract risks of TPL are evaluated through the fuzzy comprehensive evaluation method.According to the combination weights,the most important risk factor of the contract risks of TPL is choosing sub-contractors.The results are basically consistent with the facts and show that the weights determined by the combination weighting method can avoid the man-made deviations of the subjective weighting method on the one hand,and prevent results opposite to the reality brought about by the objective weighting method on the other hand.Meanwhile,the results of the fuzzy comprehensive evaluation are that the contract risks of TPL are at a high risk level.Roughly this matches real situations,and it indicates that the combination weighting method can generate the comprehensive assessment more scientifically and more reasonably as well.展开更多
Puts forward an algebraic structure of the Chaplygin's equations of nonholonomic systems, establish the Poisson's theory of the integration equations and gives an example for illustrating the application of th...Puts forward an algebraic structure of the Chaplygin's equations of nonholonomic systems, establish the Poisson's theory of the integration equations and gives an example for illustrating the application of the result.展开更多
Geometry optimization of p-C_(6)H_(4)-connected cyclo[20]carbon(p-C_(6)H_(4)-C_(20))was carried out at M062X/6-311G(d,p)level,three kinds of bond orders(Mayer,Laplacian,and Wiberg),electron-hole distributions,localize...Geometry optimization of p-C_(6)H_(4)-connected cyclo[20]carbon(p-C_(6)H_(4)-C_(20))was carried out at M062X/6-311G(d,p)level,three kinds of bond orders(Mayer,Laplacian,and Wiberg),electron-hole distributions,localized orbital locators(LOL),and infrared(IR)spectrum were also performed at the same level.Based on TD-DFT M062X/6-311G(d,p)method,the first 20 excited states and ultraviolet(UV)spectra of p-C_(6)H_(4)-C_(20) were calculated.Calculation results of π-electron delocalization analyses prove thatπ-electron delocalization of p-C_(6)H_(4)-C_(20) is more likely to occur on shorter C-C bonds rather than longer C-C bonds,and inside/outside of the ring plane rather than above/below the ring plane.Two absorption peaks of p-C_(6)H_(4)-C_(20) locate at about 319 nm and 236 nm,respectively.展开更多
VHDL and its supporting environment are active domain in the field of logic design.In the paper the design principle and some key techniques to solve the problems on the implementation of the VHDL parser are introduce...VHDL and its supporting environment are active domain in the field of logic design.In the paper the design principle and some key techniques to solve the problems on the implementation of the VHDL parser are introduced. According to the methods discussed in the paper, the VHDL parser based on VHDL IEEE 1076 standard version is implemented and a series of strict tests are done. This VHDL parser is front-end tool of the VHDL high level synthesis and mixed level simulation system developed by the Research Center of ASIC of BIT.展开更多
He’nan is a populous province in China, in which a large number of rural laborers are one of the important factors affecting social and economic development of He’nan Province. However, the employment structure and ...He’nan is a populous province in China, in which a large number of rural laborers are one of the important factors affecting social and economic development of He’nan Province. However, the employment structure and transfer characteristics of rural laborers have important effect on regional industrial structure. Applying shiftshare method( SSM) which is often used in analyzing the characteristics of industrial structure to the structure analysis of the labour force can better show the characteristics of labor employment and transfer, thereby providing some reference for the development of regional social economy. The results showed that the transfer of rural laborers in He’nan Province still showed the typical characteristics of traditional agricultural province; the basic advantages of agricultural employment of rural laborers have gradually weakened, while the competitive advantages of the industry, the building industry, and the wholesale & retail trade and catering service industry gradually became obvious, at the same time, there were differences in the deviation of departments among different regions of He’nan Province.展开更多
Using a modified C D function and stochastic frontier model, the paper analyzed China's cotton yield capacity and found that the yield and technical efficiency of China's cotton planting system can be increas...Using a modified C D function and stochastic frontier model, the paper analyzed China's cotton yield capacity and found that the yield and technical efficiency of China's cotton planting system can be increased by the use of genetically modified (GM) varieties.展开更多
Objective The aim was to provide basis for molecular marker assisted selection and resistance breeding of Langya chicken. Method The genetic polymorphism of Hae III site of Mx gene 3' sequence in Langya chicken was ...Objective The aim was to provide basis for molecular marker assisted selection and resistance breeding of Langya chicken. Method The genetic polymorphism of Hae III site of Mx gene 3' sequence in Langya chicken was analyzed by PCR-RFLP. Result The results showed that Hae III site controlled by allele A and B were polymorphic in Langya chicken breeds and the allele frequencies of A and B were 0.562 and 0.438 respectively. The genotype distribution of Hae III site was significantly not in Hardy-Weinberg equilibrium ( P 〈0.01 ). The polymorphic fragments were cloned and sequenced, and the results revealed that the fragment size was 357 bp and a deletion of 31 bp occurred in variation sequences. Conclusion It was found that Hae III-RFLP exists in Mx gene 3' sequence in Langya chicken breeds of Shandong Province.展开更多
AIM:To develop a novel endoscopic severity model of intestinal Behcet's disease(BD) and to evaluate its feasibility by comparing it with the actual disease activity index for intestinal Behcet's disease(DAIBD)...AIM:To develop a novel endoscopic severity model of intestinal Behcet's disease(BD) and to evaluate its feasibility by comparing it with the actual disease activity index for intestinal Behcet's disease(DAIBD).METHODS:We reviewed the medical records of 167 intestinal BD patients between March 1986 and April 2011.We also investigated the endoscopic parameters including ulcer locations,distribution,number,depth,shape,size and margin to identify independent factors associated with DAIBD.An endoscopic severity model was developed using significant colonoscopic variables identified by multivariate regression analysis and its correlation with the DAIBD was evaluated.To determine factors related to the discrepancy between endoscopic severity and clinical activity,clinical characteristics and laboratory markers of the patients were analyzed.RESULTS:A multivariate regression analysis revealed that the number of intestinal ulcers(≥ 2,P = 0.031) and volcanoshaped ulcers(P = 0.001) were predictive factors for the DAIBD.An endoscopic severity model(Y) was developed based on selected endoscopic variables as follows:Y = 47.44 + 9.04 × non-Ileocecal area + 11.85 ×≥ 2 of intestinal ulcers + 5.03 × shallow ulcers + 12.76 × deep ulcers + 4.47 × geographicshaped ulcers + 26.93 × volcano-shaped ulcers + 8.65 ×≥ 20 mm of intestinal ulcers.However,endoscopic parameters used in the multivariate analysis explained only 18.9% of the DAIBD variance.Patients with severe DAIBD scores but with moderately predicted disease activity by the endoscopic severity model had more symptoms of irritable bowel syndrome(21.4% vs 4.9%,P = 0.026) and a lower rate of corticosteroid use(50.0% vs 75.6%,P = 0.016) than those with severe DAIBD scores and accurately predicted disease by the model.CONCLUSION:Our study showed that the number of intestinal ulcers and volcano-shaped ulcers were predictive factors for severe DAIBD scores.However,the correlation between endoscopic severity and DAIBD(r = 0.434) was weak.展开更多
AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to ...AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to measure miR-200, ZEB1 and ZEB2 expression. Ingenuity Pathway Analysis of miR-200 targets was used to predict biological outcomes. RESULTS: Barrett's epithelium expressed lower levels of miR-141 and miR-200c than did gastric and duodenal epithelia (P < 0.001). In silico analysis indicated roles for the miR-200 family in molecular pathways that distinguish Barrett's epithelium from gastric and duodenalepithelia, and which control apoptosis and proliferation. All miR-200 members were downregulated in adenocarcinoma (P < 0.02), and miR-200c expression was also downregulated in non-invasive epithelium adjacent to adenocarcinoma (P < 0.02). The expression of all miR-200 members was lower in Barrett's epithelium derived high-grade dysplastic cell lines than in a cell line derived from benign Barrett's epithelium. We observed signif icant inverse correlations between miR-200 family expression and ZEB1 and ZEB2 expression in Barrett's epithelium and esophageal adenocarcinoma (P < 0.05). CONCLUSION: miR-200 expression might contribute to the anti-apoptotic and proliferative phenotype of Barrett's epithelium and regulate key neoplastic processes in this epithelium.展开更多
To purify the protein encoding the small capsid protein (SCP) of KSHV and analyze its immunogenicity, the carboxyl terminus of orf65 of Kaposi's sarcoma associated-herpesvirus (KSHV) was expressed in a prokaryoti...To purify the protein encoding the small capsid protein (SCP) of KSHV and analyze its immunogenicity, the carboxyl terminus of orf65 of Kaposi's sarcoma associated-herpesvirus (KSHV) was expressed in a prokaryotic expression system. The expression of recombinant E.coli containing pQE-80L-orf65 was induced by isopropyl-13-D-thiogalactopyranoside (IPTG) and the fusion protein was purified by chromatography. The expressed protein and its purified product were identified by sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) and showed that 9 kDa was the expected size of the purified orf65 protein. The antiserum was produced in rabbit which was immunized by purified orf65 protein. An ELISA assay was established to analyze the immunogenicity of the purified orf65 protein. The ELISA analysis demonstrated that orf65 protein has strong immune activity, and the immune activity of polyclonal antibody against orf65 was more than 4 fold higher than that in the serum of the non-immunized rabbit. These results demonstrate that purified orf65 protein has very strong immunogenicity and can be used in screening KSHV infection in the general population using ELISA.展开更多
文摘Aim To analyse the static temperature field ofthe solid rubber tire(SRT).Methods The mechanical and thermal FE models were developed and analyzed respectively with the FE software ANSYS.Results The maximum temperature becomes higher with the higher with the higher velocity of tire and scales down slightly with the higher convection coefficients.The mixed models are reasonable.Conclusion The study on static temperature field is important and reasonable.It gives the fundament for life analysis of SRT.
基金The National Key Technology R&D Program of China during the 11th Five-Year Plan Period(No.2006BAH02A06)
文摘First,the analytical hierarchy process(AHP),which stands for the subjective weighting method,and the entropy method,which stands for the objective weighting method,are chosen to calculate the index weights of the contract risks of third party logistics(TPL),respectively.Then,they can determine the combination weights using the combination weighting method.Second,using the combination weights,the contract risks of TPL are evaluated through the fuzzy comprehensive evaluation method.According to the combination weights,the most important risk factor of the contract risks of TPL is choosing sub-contractors.The results are basically consistent with the facts and show that the weights determined by the combination weighting method can avoid the man-made deviations of the subjective weighting method on the one hand,and prevent results opposite to the reality brought about by the objective weighting method on the other hand.Meanwhile,the results of the fuzzy comprehensive evaluation are that the contract risks of TPL are at a high risk level.Roughly this matches real situations,and it indicates that the combination weighting method can generate the comprehensive assessment more scientifically and more reasonably as well.
文摘Puts forward an algebraic structure of the Chaplygin's equations of nonholonomic systems, establish the Poisson's theory of the integration equations and gives an example for illustrating the application of the result.
文摘Geometry optimization of p-C_(6)H_(4)-connected cyclo[20]carbon(p-C_(6)H_(4)-C_(20))was carried out at M062X/6-311G(d,p)level,three kinds of bond orders(Mayer,Laplacian,and Wiberg),electron-hole distributions,localized orbital locators(LOL),and infrared(IR)spectrum were also performed at the same level.Based on TD-DFT M062X/6-311G(d,p)method,the first 20 excited states and ultraviolet(UV)spectra of p-C_(6)H_(4)-C_(20) were calculated.Calculation results of π-electron delocalization analyses prove thatπ-electron delocalization of p-C_(6)H_(4)-C_(20) is more likely to occur on shorter C-C bonds rather than longer C-C bonds,and inside/outside of the ring plane rather than above/below the ring plane.Two absorption peaks of p-C_(6)H_(4)-C_(20) locate at about 319 nm and 236 nm,respectively.
文摘VHDL and its supporting environment are active domain in the field of logic design.In the paper the design principle and some key techniques to solve the problems on the implementation of the VHDL parser are introduced. According to the methods discussed in the paper, the VHDL parser based on VHDL IEEE 1076 standard version is implemented and a series of strict tests are done. This VHDL parser is front-end tool of the VHDL high level synthesis and mixed level simulation system developed by the Research Center of ASIC of BIT.
基金Supported by the Youth Program of National Social Science Fund(12CJL073)the Youth Program of Humanities and Social Science Research of Ministry of Education(11YJC790272)~~
文摘He’nan is a populous province in China, in which a large number of rural laborers are one of the important factors affecting social and economic development of He’nan Province. However, the employment structure and transfer characteristics of rural laborers have important effect on regional industrial structure. Applying shiftshare method( SSM) which is often used in analyzing the characteristics of industrial structure to the structure analysis of the labour force can better show the characteristics of labor employment and transfer, thereby providing some reference for the development of regional social economy. The results showed that the transfer of rural laborers in He’nan Province still showed the typical characteristics of traditional agricultural province; the basic advantages of agricultural employment of rural laborers have gradually weakened, while the competitive advantages of the industry, the building industry, and the wholesale & retail trade and catering service industry gradually became obvious, at the same time, there were differences in the deviation of departments among different regions of He’nan Province.
文摘Using a modified C D function and stochastic frontier model, the paper analyzed China's cotton yield capacity and found that the yield and technical efficiency of China's cotton planting system can be increased by the use of genetically modified (GM) varieties.
基金Supported by Shandong Project of Agricultural Improved VarietyEngineering~~
文摘Objective The aim was to provide basis for molecular marker assisted selection and resistance breeding of Langya chicken. Method The genetic polymorphism of Hae III site of Mx gene 3' sequence in Langya chicken was analyzed by PCR-RFLP. Result The results showed that Hae III site controlled by allele A and B were polymorphic in Langya chicken breeds and the allele frequencies of A and B were 0.562 and 0.438 respectively. The genotype distribution of Hae III site was significantly not in Hardy-Weinberg equilibrium ( P 〈0.01 ). The polymorphic fragments were cloned and sequenced, and the results revealed that the fragment size was 357 bp and a deletion of 31 bp occurred in variation sequences. Conclusion It was found that Hae III-RFLP exists in Mx gene 3' sequence in Langya chicken breeds of Shandong Province.
文摘AIM:To develop a novel endoscopic severity model of intestinal Behcet's disease(BD) and to evaluate its feasibility by comparing it with the actual disease activity index for intestinal Behcet's disease(DAIBD).METHODS:We reviewed the medical records of 167 intestinal BD patients between March 1986 and April 2011.We also investigated the endoscopic parameters including ulcer locations,distribution,number,depth,shape,size and margin to identify independent factors associated with DAIBD.An endoscopic severity model was developed using significant colonoscopic variables identified by multivariate regression analysis and its correlation with the DAIBD was evaluated.To determine factors related to the discrepancy between endoscopic severity and clinical activity,clinical characteristics and laboratory markers of the patients were analyzed.RESULTS:A multivariate regression analysis revealed that the number of intestinal ulcers(≥ 2,P = 0.031) and volcanoshaped ulcers(P = 0.001) were predictive factors for the DAIBD.An endoscopic severity model(Y) was developed based on selected endoscopic variables as follows:Y = 47.44 + 9.04 × non-Ileocecal area + 11.85 ×≥ 2 of intestinal ulcers + 5.03 × shallow ulcers + 12.76 × deep ulcers + 4.47 × geographicshaped ulcers + 26.93 × volcano-shaped ulcers + 8.65 ×≥ 20 mm of intestinal ulcers.However,endoscopic parameters used in the multivariate analysis explained only 18.9% of the DAIBD variance.Patients with severe DAIBD scores but with moderately predicted disease activity by the endoscopic severity model had more symptoms of irritable bowel syndrome(21.4% vs 4.9%,P = 0.026) and a lower rate of corticosteroid use(50.0% vs 75.6%,P = 0.016) than those with severe DAIBD scores and accurately predicted disease by the model.CONCLUSION:Our study showed that the number of intestinal ulcers and volcano-shaped ulcers were predictive factors for severe DAIBD scores.However,the correlation between endoscopic severity and DAIBD(r = 0.434) was weak.
基金Supported by National Health and Medical Research Council, Australia
文摘AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to measure miR-200, ZEB1 and ZEB2 expression. Ingenuity Pathway Analysis of miR-200 targets was used to predict biological outcomes. RESULTS: Barrett's epithelium expressed lower levels of miR-141 and miR-200c than did gastric and duodenal epithelia (P < 0.001). In silico analysis indicated roles for the miR-200 family in molecular pathways that distinguish Barrett's epithelium from gastric and duodenalepithelia, and which control apoptosis and proliferation. All miR-200 members were downregulated in adenocarcinoma (P < 0.02), and miR-200c expression was also downregulated in non-invasive epithelium adjacent to adenocarcinoma (P < 0.02). The expression of all miR-200 members was lower in Barrett's epithelium derived high-grade dysplastic cell lines than in a cell line derived from benign Barrett's epithelium. We observed signif icant inverse correlations between miR-200 family expression and ZEB1 and ZEB2 expression in Barrett's epithelium and esophageal adenocarcinoma (P < 0.05). CONCLUSION: miR-200 expression might contribute to the anti-apoptotic and proliferative phenotype of Barrett's epithelium and regulate key neoplastic processes in this epithelium.
基金Knowledge Innovation Program of the Chinese Academy of Sciences(0702121Y-J1)
文摘To purify the protein encoding the small capsid protein (SCP) of KSHV and analyze its immunogenicity, the carboxyl terminus of orf65 of Kaposi's sarcoma associated-herpesvirus (KSHV) was expressed in a prokaryotic expression system. The expression of recombinant E.coli containing pQE-80L-orf65 was induced by isopropyl-13-D-thiogalactopyranoside (IPTG) and the fusion protein was purified by chromatography. The expressed protein and its purified product were identified by sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) and showed that 9 kDa was the expected size of the purified orf65 protein. The antiserum was produced in rabbit which was immunized by purified orf65 protein. An ELISA assay was established to analyze the immunogenicity of the purified orf65 protein. The ELISA analysis demonstrated that orf65 protein has strong immune activity, and the immune activity of polyclonal antibody against orf65 was more than 4 fold higher than that in the serum of the non-immunized rabbit. These results demonstrate that purified orf65 protein has very strong immunogenicity and can be used in screening KSHV infection in the general population using ELISA.