A study was performed on the long-term effect of straw incorporation on soil microbial biomass C contents, C and N dynamics in both Rothamsted and Woburn soils. The results showed that for both soils,the microbial bio...A study was performed on the long-term effect of straw incorporation on soil microbial biomass C contents, C and N dynamics in both Rothamsted and Woburn soils. The results showed that for both soils,the microbial biomass C contents were significantly different among all the treatments, and followed the sequence in treatments of straw chopped and incorporated into 10 cm (CI10) > straw burnt and incorporated into 10 cm (BI10) > straw chopped and incorporated into 20 cm (CI20) > straw burnt and incorporated into 20 cm (BI20). Laboratory incubation of soils showed that the cumulative CO2 evolution was closely related to the soil microbial biomass C content. Carbon dioxide evolution rates (CO2-C, μg (g d) -1 ) decreased rapidly in the first two weeks’ incubation, then decreased more slowly. The initial K2SO4-extractable NH4-N and NO3-N contents were low and similar in all the treatments, and all increased gradually with the incubation time. However, net N immobilization was observed in chopped treatments for Rothamsted soils during the first 4 weeks. Nevertheless, more N mineralization occurred in neatment CI10 than any other treatment at the end of incubation for both soils. The Woburn soils could more easily suffer from the leaching of nitrate because the soils were more permeable and more N was mineralized during the incubation compared to the Rothamsted soils.展开更多
At hospital, nutrition represents an important value of care, particularly for patients at risk. However, it is observed that for various reasons, the restoration of the hospitalized patients is often neglected in the...At hospital, nutrition represents an important value of care, particularly for patients at risk. However, it is observed that for various reasons, the restoration of the hospitalized patients is often neglected in the privileges of the medical care. The establishment of a Liaison Committee of Food and Nutrition （LCFN） within a health establishment has shown according to several works, its positive impact in improving the hygienic quality and nutritional dishes served to patients. In this framework, we conducted a quasi-experimental study into CHU F hached Sousse of Tunisia type （before/after, creation LCFN） in order to assess the role of such structure （LCFN） in the qualification and the improvement of patients＇ food. Our study was based during the two phases （2007/2010） on the same methodology. Thus we have conducted an audit of observation of hygiene practices along the distribution chain of patient＇s meals and the samples for microbiological analyzes from food, surfaces, equipment and personnel＇s hands.The results obtained have shown, in one hand, a degradation of the average rate of contamination for the bacteriological analyzes, and in the other hand, an evolution of the rate of hygiene standards respected. According to this study, the role played by the LCFN is becoming more and clearer in the improvement of the hygienic quality of patients＇ dishes without forgetting the impact of improving the nutritional quality and Hedonics.展开更多
0027018 病人佩戴垂直向屈光参差眼镜可扩大垂直向融合调幅/Griebel S R//J AmAcad Ophthalmol.-1999,106(9).-1731～1733 北医图0027019 长期佩戴一次性水凝胶的副作用:佩带接触镜头13个月的结果/Sankaridurg P R//J Am Acad Ophthalmo...0027018 病人佩戴垂直向屈光参差眼镜可扩大垂直向融合调幅/Griebel S R//J AmAcad Ophthalmol.-1999,106(9).-1731～1733 北医图0027019 长期佩戴一次性水凝胶的副作用:佩带接触镜头13个月的结果/Sankaridurg P R//J Am Acad Ophthalmol.-1999,106(9).-1671～1680 北医图0027020展开更多
Malaria is one of the leading causes of consultation in African pediatric hospitals. In Gabon, malaria transmission is perennial. Plasmodium falciparum, responsible for the most severe form of the disease, represents ...Malaria is one of the leading causes of consultation in African pediatric hospitals. In Gabon, malaria transmission is perennial. Plasmodium falciparum, responsible for the most severe form of the disease, represents more than 95% of all species. In P. falciparum infection, the hyperparasitemia accounts among the main criteria of disease severity. However, in many endemic countries, a significant decrease of malaria burden accompanying with the diminution of parasite load in infected individuals has been demonstrated. The objective of the study was to analyze the occurrence of febrile syndrome in childhood and investigate whether the acute febrile illness could be associated with P. falciparum submicroscopic infection. A cross-sectional study was carried out during January to March 2013 in Franceville. A total of 203 acute febrile children were enrolled. A clinical examination and biomedical analysis including parasitological diagnosis by microscope were carried out in all the patients and PCR on microscope negative ones. Of 203 children recruited for febrile syndrome, 111 have been diagnosed positive for P. falciparum infection, 73 （35.9%） by microscope （ME） and 38 （18.71%） by PCR （submicroscopic infection = SM1） with an overall prevalence of 54.68%. Of the 11 1 P. falciparum infected individuals and according to the WHO criteria, 35 （31.53%） children showed a clinical picture of severe malaria against 76 （68.47%） others classified as uncomplicated malaria. The overall prevalence rates were therefore estimated as 17.24 （35/203） for severe cases and 37.43% （76/203） for uncomplicated ones. Clinically, these severe malaria cases （27 ME＋ and 8 PCR＋） were mainly composed of 85.71% of anaemic patients （30/35）, 71.14% of prostrated individuals （25/35） and 57.14% of children with clinical icterus （20/35）. However, only two cases of severe anaemia were observed, the remaining others cases were moderate （10） and mild anaemia （18）. More interestingly, eight submicroscopic infected patients （22.85%） were found with neurological manifestations （prostration） and all were experiencing thrombocytopenia. Lastly, 1 hyperparasitemia, 6 hypoglycemia and 2 respiratory distresses were also observed among these severe malaria cases. P. falciparum submicroscopic infection may lead to severe malaria in perennial transmission area.展开更多
In the present study, a simple and reliable HPLC-UV method was developed for the determination of mefunidone. The bioanalytical specific procedure involved extraction of mefunidone from a 500-μL hepatic microsomal sy...In the present study, a simple and reliable HPLC-UV method was developed for the determination of mefunidone. The bioanalytical specific procedure involved extraction of mefunidone from a 500-μL hepatic microsomal system through protein precipitation by methanol. Chromatographic separation was achieved using an Agilent TC-C（18） column（4.6 mm×250 mm, 5 μm） with an isocratic mobile phase consisting of 10 mM ammonium formate（pH 2.9, later adjusted by using 10% formic acid）–acetonitrile（70：30, v/v） at a flow rate of 1.0 mL/min. The UV detection wavelength was set at 245 nm. Mefunidone and pirfenidone（PFD, internal standard, IS） were eluted at 6.0 and 9.7 min, separately, with the total running time of 12 min. According to US Food and Drug Administration bioanalytical guidelines, method validation was performed, and the results met the acceptance criteria in details. The calibration curve of mefunidone in liver microsomes was linear over the concentration range of 0.5–16 μg.mL^（–1）. Intra-and inter-run precisions of mefunidone were less than 9.0%, and the biases were within ±10.0%. After incubation of mefunidone in liver microsomes, this method was successfully applied to the pharmacokinetic study.展开更多
文摘A study was performed on the long-term effect of straw incorporation on soil microbial biomass C contents, C and N dynamics in both Rothamsted and Woburn soils. The results showed that for both soils,the microbial biomass C contents were significantly different among all the treatments, and followed the sequence in treatments of straw chopped and incorporated into 10 cm (CI10) > straw burnt and incorporated into 10 cm (BI10) > straw chopped and incorporated into 20 cm (CI20) > straw burnt and incorporated into 20 cm (BI20). Laboratory incubation of soils showed that the cumulative CO2 evolution was closely related to the soil microbial biomass C content. Carbon dioxide evolution rates (CO2-C, μg (g d) -1 ) decreased rapidly in the first two weeks’ incubation, then decreased more slowly. The initial K2SO4-extractable NH4-N and NO3-N contents were low and similar in all the treatments, and all increased gradually with the incubation time. However, net N immobilization was observed in chopped treatments for Rothamsted soils during the first 4 weeks. Nevertheless, more N mineralization occurred in neatment CI10 than any other treatment at the end of incubation for both soils. The Woburn soils could more easily suffer from the leaching of nitrate because the soils were more permeable and more N was mineralized during the incubation compared to the Rothamsted soils.
文摘At hospital, nutrition represents an important value of care, particularly for patients at risk. However, it is observed that for various reasons, the restoration of the hospitalized patients is often neglected in the privileges of the medical care. The establishment of a Liaison Committee of Food and Nutrition （LCFN） within a health establishment has shown according to several works, its positive impact in improving the hygienic quality and nutritional dishes served to patients. In this framework, we conducted a quasi-experimental study into CHU F hached Sousse of Tunisia type （before/after, creation LCFN） in order to assess the role of such structure （LCFN） in the qualification and the improvement of patients＇ food. Our study was based during the two phases （2007/2010） on the same methodology. Thus we have conducted an audit of observation of hygiene practices along the distribution chain of patient＇s meals and the samples for microbiological analyzes from food, surfaces, equipment and personnel＇s hands.The results obtained have shown, in one hand, a degradation of the average rate of contamination for the bacteriological analyzes, and in the other hand, an evolution of the rate of hygiene standards respected. According to this study, the role played by the LCFN is becoming more and clearer in the improvement of the hygienic quality of patients＇ dishes without forgetting the impact of improving the nutritional quality and Hedonics.
文摘0027018 病人佩戴垂直向屈光参差眼镜可扩大垂直向融合调幅/Griebel S R//J AmAcad Ophthalmol.-1999,106(9).-1731～1733 北医图0027019 长期佩戴一次性水凝胶的副作用:佩带接触镜头13个月的结果/Sankaridurg P R//J Am Acad Ophthalmol.-1999,106(9).-1671～1680 北医图0027020
文摘Malaria is one of the leading causes of consultation in African pediatric hospitals. In Gabon, malaria transmission is perennial. Plasmodium falciparum, responsible for the most severe form of the disease, represents more than 95% of all species. In P. falciparum infection, the hyperparasitemia accounts among the main criteria of disease severity. However, in many endemic countries, a significant decrease of malaria burden accompanying with the diminution of parasite load in infected individuals has been demonstrated. The objective of the study was to analyze the occurrence of febrile syndrome in childhood and investigate whether the acute febrile illness could be associated with P. falciparum submicroscopic infection. A cross-sectional study was carried out during January to March 2013 in Franceville. A total of 203 acute febrile children were enrolled. A clinical examination and biomedical analysis including parasitological diagnosis by microscope were carried out in all the patients and PCR on microscope negative ones. Of 203 children recruited for febrile syndrome, 111 have been diagnosed positive for P. falciparum infection, 73 （35.9%） by microscope （ME） and 38 （18.71%） by PCR （submicroscopic infection = SM1） with an overall prevalence of 54.68%. Of the 11 1 P. falciparum infected individuals and according to the WHO criteria, 35 （31.53%） children showed a clinical picture of severe malaria against 76 （68.47%） others classified as uncomplicated malaria. The overall prevalence rates were therefore estimated as 17.24 （35/203） for severe cases and 37.43% （76/203） for uncomplicated ones. Clinically, these severe malaria cases （27 ME＋ and 8 PCR＋） were mainly composed of 85.71% of anaemic patients （30/35）, 71.14% of prostrated individuals （25/35） and 57.14% of children with clinical icterus （20/35）. However, only two cases of severe anaemia were observed, the remaining others cases were moderate （10） and mild anaemia （18）. More interestingly, eight submicroscopic infected patients （22.85%） were found with neurological manifestations （prostration） and all were experiencing thrombocytopenia. Lastly, 1 hyperparasitemia, 6 hypoglycemia and 2 respiratory distresses were also observed among these severe malaria cases. P. falciparum submicroscopic infection may lead to severe malaria in perennial transmission area.
基金The National Natural Science Foundation of China(Grant No.81302819),the National Natural Science Foundation of China(Grant No.C0709-31201056)the Fundamental Research Funds for the Central Universities of China(Grant No.7601110179)the Fundamental Research Funds for the Central south Universities of China(Grant No.2016zzts494)
文摘In the present study, a simple and reliable HPLC-UV method was developed for the determination of mefunidone. The bioanalytical specific procedure involved extraction of mefunidone from a 500-μL hepatic microsomal system through protein precipitation by methanol. Chromatographic separation was achieved using an Agilent TC-C（18） column（4.6 mm×250 mm, 5 μm） with an isocratic mobile phase consisting of 10 mM ammonium formate（pH 2.9, later adjusted by using 10% formic acid）–acetonitrile（70：30, v/v） at a flow rate of 1.0 mL/min. The UV detection wavelength was set at 245 nm. Mefunidone and pirfenidone（PFD, internal standard, IS） were eluted at 6.0 and 9.7 min, separately, with the total running time of 12 min. According to US Food and Drug Administration bioanalytical guidelines, method validation was performed, and the results met the acceptance criteria in details. The calibration curve of mefunidone in liver microsomes was linear over the concentration range of 0.5–16 μg.mL^（–1）. Intra-and inter-run precisions of mefunidone were less than 9.0%, and the biases were within ±10.0%. After incubation of mefunidone in liver microsomes, this method was successfully applied to the pharmacokinetic study.
