原发性淀粉样变性的临床病理分析及电镜观察

对１例典型的原发性淀粉样变性病例进行常规病理、组织化学检查及电镜观察，并结合文献对该病进行临床病理分析及鉴别诊断。结果认为本病是一种较为罕见而易误诊的疾病，病理、组化及电镜观察对本病的确诊有着重要的意义，尤其是高锰酸钾刚果红染色是区别原发或继发性淀粉样变性一种好方法。

Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers

Sea cucumber Apostichopus japonicus samples were collected in Changdao, Penglai (PL), 27 individuals, and Lingshandao, Qingdao (QD), 30 individuals, in the Shandong Peninsula, China. Ten SSR primers were used to assess the genetic variation and relationship between and within the two stocks. Respectively, for each stock, the percentage of polymorphic bands was 85.2% and 86.9%; the gene diver- sity was 0.360 5 and 0.342 8; and the Shannon’s information index was 0.515 0 and 0.499 0. At species level, the percentage of polymorphic bands was 92.2%, the total gene diversity was 0.378 9 and the Shannon’s information index was 0.550 4. The coefficient of overall genetic differentiation and the ge- netic distances between the stocks were also calculated to be 0.073 0 and 0.079 6 using the POPGENE program. Results show that the genetic diversity of the two stocks is still large but the genetic distance between the two stocks is close. A dendrogram was constructed for the 57 individuals from the two stocks, showing that the genetic structure was unitary for PL stock but complex for QD stock.

Microsatellite analysis of variation among wild, domesticated, and genetically improved populations of blunt snout bream （Megalobrama amblycephala）

In the present study, the genetic diversity of one selected strain （Pujiang No. 1）, two domesticated populations （GA and HX） and four wild populations （LZ, YN, SS and JL） of blunt snout bream （Megalobrama amblycephala） was analyzed using 17 microsatellite markers. The results showed that an average of 4.88-7.65 number of alleles （A）; an average of 3.20-5.33 effective alleles （Ne）; average observed beterozygosity （Ho） of 0.6985-0.9044; average expected beterozygosity （He） of 0.6501--0.7805; and the average polymorphism information content （PIC） at 0.5706-0.7226. Pairwise FST value between populations ranged from 0.0307-0.1451, and Nei＇s standard genetic distance between populations was 0.0938-0.4524. The expected heterozygosities in the domesticated populations （GA and HX） were significantly lower than those found in three wild populations （LZ, SS and JL）, but no difference was detected when compared with the wild YN population. Likewise, no difference was found between the four wild populations or two domesticated populations. The expected heterozygosity in Pujiang No. 1 was higher than the two domesticated populations and lower than the four wild populations. Regarding pairwise Fsr value between populations, permutation test P-values were significant between the GA, HX and PJ populations, but not between the four wild populations. These results showed that the expected beterozygosity in the selected strain of blunt snout bream, after seven generations of selective breeding, was lower than that of wild populations, but this strain retains higher levels of genetic diversity than domesticated populations. The genetic differences and differentiation amongst wild populations, domesticated populations and the genetically improved strain of blunt snout bream will provide important conservation criteria and guide the utilization of germplasm resources.

Anal metastasis from recurrent breast lobular carcinoma:A case report

We report a case of isolated gastrointestinal metastasis from breast Iobular carcinoma, which mimicked primary anal cancer. In July 2000, an 88-year-old woman presented with infiltrating Iobular cancer （pT1/G2/N2）. The patient received postoperative radiotherapy and hormonal therapy. Four years later, she presented with an anal polypoid lesion. The mass was removed for biopsy. Immunohistochemical staining suggested a breast origin. Radiotherapy was chosen for this patient, which resulted in complete regression of the lesion. The patient died 3 years after the first manifestation of gastrointestinal metastasis. According to the current literature, we consider the immunohistochemistry features that are essential to support the suspicion of gastrointestinal breast metastasis, and since we consider the gastrointestinal involvement as a sign of systemic disease, the therapy should be less aggressive and systemic.

Mutagenic Effect of Three Invasive Species through Allium Cepa Bioassay

Invasive alien species are a global threat to biodiversity that affects protected areas around the world. The occupation of new environments by these plants is a problem to be solved and it is essential to investigate all the aspects that allow this successful to find solutions to this question, such as its mutagenic effects. Thus, this study aimed to evaluate the mutagenic effect of leaves extracts of Acacia mangium Willd, Artocarpus heterophyllus Lam and Eriobothrya japonica （Thunb.） Lindl through Allium cepa bioassay. For this, A. cepa seeds were submitted to continuous and discontinuous （acute and chronic） treatments in medium with water （negative control） or four concentration of each extract （1, 5, 10 and 50 mg/mL）. The mitotic index was affected at all concentrations of three extracts tested in all treatments, continuous and discontinuous. Aneugenic effects were not related to any treatment tested. E. japonica extract induced clastogenic effects at 1, 5 and 10 mg/mL in continuous treatment, 5 and 10 mg/mL in acute discontinuous treatment and at 10 mg/mL in chronic discontinuous treatment. Clastogenic effect was also observed at 10 mg/mL ofA. heterophyllus extract in continuous and acute discontinuous treatments.

Bird Species Diversity and Spatio-temporal Variation in the Sihong Hongze Lake Wetland National Nature Reserve in Eastern China

As an important hub on the East Asian-Australian Flyway(EAAF),Hongze Lake is an important migratory stopover and wintering site for hundreds of thousands of birds.However,research on bird community diversity in this area is still lacking.We conducted a bird survey from July 2020 to June 2021 using the line transect method on the terrestrial habitat,as well as the fixed-point method in the lake wetland at the Sihong Hongze Lake Wetland National Nature Reserve located in northwestern Hongze Lake,and analyzed the temporal-spatial variation in the bird community.The results showed that a total of 170432 detections of 215 bird species belonging to 18 orders and 55 families were recorded.In terms of species composition,the proportion of terrestrial birds was relatively high,followed by waterfowl richness,with high numbers of Anatidae species and shorebirds.For bird species abundance,waterfowl had the highest abundance of common coot(Fulica atra),belonging to the Rallidae family,followed by Anatidae and Ardeidae species.The abundance of shorebirds was extremely low,and that of non-waterfowl was also low.In terms of temporal patterns,the number of bird species and richness index were higher in spring and autumn but lower in winter and summer.The bird abundance was the highest in winter,and the remaining three seasons were similar in terms of bird abundance.The diversity index and evenness index were higher in spring,summer and autumn,and lowest in winter.For the spatial pattern,the open water in the western part of the reserve included the most densely distributed areas for birds,and the number of bird species and their abundance were both the highest in that part.There were significant differences in the bird community structures among the four habitats.The species number and richness index of birds in the reed habitat were the highest,and the bird abundance was also high,but the diversity index and evenness index were low.Although the bird abundance in the lake habitat was much higher than in the other habitats,the diversity index,evenness index and richness index were the lowest.The numbers of bird species and individuals in tourist attraction land and farmland were low,but the diversity index,evenness index and richness index were high.Our results reveal the spatial and temporal patterns of bird species diversity and abundance in Sihong Hongze Lake Wetland National Nature Reserve,and reflect the effects of different habitat types on bird diversity.