The objectives of this study were to understand the effects of different electrical and reactor＇s parameters on the removal efficiency of air pollution and to optimize the parameters to achieve the highest removal ef...The objectives of this study were to understand the effects of different electrical and reactor＇s parameters on the removal efficiency of air pollution and to optimize the parameters to achieve the highest removal efficiency. The surface discharge was applied to dielectric barrier discharge reactors and the voltage, current, frequency and the electrode shape were employed to the reactor as main variables. In general, it has been known that the removal efficiency is decided by the applied power, which is calculated by multiplication of the applied voltage and current. However, the removal efficiency was significantly changed by the composition ratio of the applied voltage and current even though the experimental conditions were not changed with the same applied power. It means that the removal effectiveness should be affected not by the discharge power but by the applied voltage and current separately. From the experimental and statistical analysis, the discharge current was the most important factor to control the removal efficiency regardless of different types of the reactors and the frequency is not the direct function to control the removal rate.展开更多
Secreted proteins are important sources for early detection and diagnosis of disease, and as such have received considerable attention. The extraction of low concentration proteins from large volumes of culture media,...Secreted proteins are important sources for early detection and diagnosis of disease, and as such have received considerable attention. The extraction of low concentration proteins from large volumes of culture media, which are rich in salts and other compounds that interfere with most proteomics techniques, presents a problem for secretome studies. Ultrafiltration, precipitation, and dialysis are three major extraction methods that can be used to overcome this problem. The present study for the first time, compared the merits and shortcomings of these three methods, without bias. Centrifugal ultrafiltration provided the best extraction efficiency, and precipitation provided the highest number of identifiable proteins. The three methods yielded closely related, but different, information on the secretome; thus, they should be considered complementary or, at least, supplementary methods. Three hundred and sixty unique proteins were identified, including 211 potential secreted proteins. Compared with previous studies, this study also identified 42 new secreted proteins. The present study not only offers a reference for the selection of secretome extraction methods, but also expands the secretome database for the investigation of hepatocellular carcinoma.展开更多
文摘The objectives of this study were to understand the effects of different electrical and reactor＇s parameters on the removal efficiency of air pollution and to optimize the parameters to achieve the highest removal efficiency. The surface discharge was applied to dielectric barrier discharge reactors and the voltage, current, frequency and the electrode shape were employed to the reactor as main variables. In general, it has been known that the removal efficiency is decided by the applied power, which is calculated by multiplication of the applied voltage and current. However, the removal efficiency was significantly changed by the composition ratio of the applied voltage and current even though the experimental conditions were not changed with the same applied power. It means that the removal effectiveness should be affected not by the discharge power but by the applied voltage and current separately. From the experimental and statistical analysis, the discharge current was the most important factor to control the removal efficiency regardless of different types of the reactors and the frequency is not the direct function to control the removal rate.
基金supported by the National Natural Science Foundation of China (Grant No. 209750240)the National Basic Research Program of China (Grant No. 2010CB912700)
文摘Secreted proteins are important sources for early detection and diagnosis of disease, and as such have received considerable attention. The extraction of low concentration proteins from large volumes of culture media, which are rich in salts and other compounds that interfere with most proteomics techniques, presents a problem for secretome studies. Ultrafiltration, precipitation, and dialysis are three major extraction methods that can be used to overcome this problem. The present study for the first time, compared the merits and shortcomings of these three methods, without bias. Centrifugal ultrafiltration provided the best extraction efficiency, and precipitation provided the highest number of identifiable proteins. The three methods yielded closely related, but different, information on the secretome; thus, they should be considered complementary or, at least, supplementary methods. Three hundred and sixty unique proteins were identified, including 211 potential secreted proteins. Compared with previous studies, this study also identified 42 new secreted proteins. The present study not only offers a reference for the selection of secretome extraction methods, but also expands the secretome database for the investigation of hepatocellular carcinoma.
