Each secretion （See） machinery minimally consists of three integral inner membrane pro- teins SecYEG. In order to know the distribution pattern of the Sec complex in Streptococcus mutans, we explored the subcellular...Each secretion （See） machinery minimally consists of three integral inner membrane pro- teins SecYEG. In order to know the distribution pattern of the Sec complex in Streptococcus mutans, we explored the subcellular localization of SecY. The anti-secY antibody was examined on Western blots to evaluate specificity. An indirect post-embedding immunogold method was used to determine the subcellular localization of the SecY in the cytoplasmic membrane of the Streptococcus mutans GS-5. lmmunoblotting experiments showed that the anti-seeY antibody specifically recognized a single band of about 47.8 kDa. Immunogold electron microscopy image of section with anti-secY antibody revealed a single intense focus of gold particles at a discrete location on the cytoplasmic membrane of the Streptococcus mutans GS-5. SecY clustered to an asymmetric microdomain, which indicated for the first time that See complex presented a unisite on the cytoplasmic membrane of Streptococcus mutans.展开更多
基金This work was supported by National Natural Science Foundation of China(30670115).
文摘Each secretion （See） machinery minimally consists of three integral inner membrane pro- teins SecYEG. In order to know the distribution pattern of the Sec complex in Streptococcus mutans, we explored the subcellular localization of SecY. The anti-secY antibody was examined on Western blots to evaluate specificity. An indirect post-embedding immunogold method was used to determine the subcellular localization of the SecY in the cytoplasmic membrane of the Streptococcus mutans GS-5. lmmunoblotting experiments showed that the anti-seeY antibody specifically recognized a single band of about 47.8 kDa. Immunogold electron microscopy image of section with anti-secY antibody revealed a single intense focus of gold particles at a discrete location on the cytoplasmic membrane of the Streptococcus mutans GS-5. SecY clustered to an asymmetric microdomain, which indicated for the first time that See complex presented a unisite on the cytoplasmic membrane of Streptococcus mutans.
