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小麦远缘杂交后代节燕98-2分离类型贮藏蛋白的研究 被引量:1
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作者 吴春太 徐如宏 张庆勤 《种子》 CSCD 北大核心 2004年第1期3-5,30,共4页
采用A PAGE和SDS PAGE聚丙烯酰胺凝胶电泳方法 ,对远缘组合分离出来的节燕 98 2类型入选 11个遗传性基本稳定的具有高产、多抗的小麦株系的醇溶蛋白和高分子量谷蛋白亚基进行了分析。结果表明 ,在A PAGE电泳分析中 ,11个供试株系具有 1... 采用A PAGE和SDS PAGE聚丙烯酰胺凝胶电泳方法 ,对远缘组合分离出来的节燕 98 2类型入选 11个遗传性基本稳定的具有高产、多抗的小麦株系的醇溶蛋白和高分子量谷蛋白亚基进行了分析。结果表明 ,在A PAGE电泳分析中 ,11个供试株系具有 11种不同的醇溶蛋白带型。在SDS FAGE电泳分析中 ,出现了 7种不同的高分子量谷蛋白亚基(HMW GS)及 6种亚基组合类型 ,优质亚基及亚基组合所占的比例较少 ,品质评分偏低 ,其变幅为 5~ 8分 ,平均为 6 3 6分。但在所分析的材料中 ,出现了一个少见的特殊亚基 :2 +10 +12。并研究了这些HMW GS和组合频率及特点。 11个株系中 7个具有 45 +10优质亚基和 2个具有 2 亚基 ,它们可供小麦优质育种利用。研究表明 ,通过远缘杂交能够选育出具有高产、抗病和优质的小麦新材料。 展开更多
关键词 小麦 远缘杂交后代 节燕98—2 分离类型 贮藏蛋白 遗传性 电泳分析
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应用ISSR和SRAP标记构建李遗传连锁图谱 被引量:1
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作者 孙莉莉 彭丽娜 +2 位作者 李铮 侯睿宁 牟蕴慧 《广东农业科学》 CAS 2022年第3期40-48,共9页
【目的】李(Prunus salicina L.)是我国东北地区极具经济开发前景的树种,具有重要的社会、经济价值。通过分子标记技术对李进行遗传连锁图谱构建,为李新品种选育提供理论基础。【方法】以吉林6号和龙园秋李为作图亲本,利用SSR引物对F_(1... 【目的】李(Prunus salicina L.)是我国东北地区极具经济开发前景的树种,具有重要的社会、经济价值。通过分子标记技术对李进行遗传连锁图谱构建,为李新品种选育提供理论基础。【方法】以吉林6号和龙园秋李为作图亲本,利用SSR引物对F_(1)子代进行真实性鉴定,经多态性筛选,选出14条ISSR引物和16对SRAP引物用于遗传连锁图谱的构建,应用Join Map 4.0软件,结合“双假测交”理论构建李遗传连锁图谱。【结果】试验获得72个真实性F_(1)子代,ISSR引物和SRAP引物在F_(1)群体产生5种分离类型、120个标记位点,经软件分析获得1张拥有16个连锁群的遗传连锁图谱,该图谱总长度为528.5 cM,包含38个分子标记位点,连锁群平均长度为13.9 cM,最长的连锁群为71.1 cM、最短的连锁群为9.6 cM,多态性标记间最大遗传距离为52.8 cM、最小遗传距离为1.4 cM。【结论】研究获得的李遗传连锁图谱丰富了寒地李的遗传研究基础,有助于李QTL定位、分子标记辅助育种和基因定位研究。 展开更多
关键词 遗传图谱构建 分子标记 多态性分析 分离类型 遗传距离
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上季落田谷的田间表现、产生原因及防止措施 被引量:2
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作者 冯生强 《四川农业科技》 2015年第10期10-11,共2页
机收、稻草覆盖、简化旱育秧会导致上季落田谷在秧母田中随种子一同萌发、生长。移栽到大田后,在水稻生产的中后期表现出明显的分离类型。通过对秧母田土的深翻,对苗床地表土、覆土中的上季落田谷进行分离,采取提前淹水促萌、人工拔除... 机收、稻草覆盖、简化旱育秧会导致上季落田谷在秧母田中随种子一同萌发、生长。移栽到大田后,在水稻生产的中后期表现出明显的分离类型。通过对秧母田土的深翻,对苗床地表土、覆土中的上季落田谷进行分离,采取提前淹水促萌、人工拔除、化除灭活等措施,能有效减少落田谷随种子一同生长的可能,直至在大田生产中无明显表现。 展开更多
关键词 落田谷 分离类型 灭活处理
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Isolation and Identification of a Subgroup A Avian Leukosis Virus from Imported Meat-type Grand-parent Chickens 被引量:28
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作者 Qing-chan ZHANG Dong-min ZHAO Hui-jun GUO Zhi-zhong CUI 《Virologica Sinica》 SCIE CAS CSCD 2010年第2期130-136,共7页
An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced a... An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence comparisons indicated that SDAU09C1 fell into subgroup A; it had homology of 88.8%-90.3% to 6 reference strains of subgroup A, much higher compared to other subgroups including subgroup B. This is the first report for ALV of subgroup A isolated from imported breeders. 展开更多
关键词 Avian leukosis virus(ALV) Subgroup A Envelope gp85 Imported Chicken Breeders
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A Note on "Doubly Periodic Propagating Wave for (2+1)-Dimensional Breaking Soliton Equation"
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作者 ZHENG Chun-Long PAN Zhen-Huan MA Zheng-Yi 《Communications in Theoretical Physics》 SCIE CAS CSCD 2009年第1期79-80,共2页
In a recent paper [Commun. Theor. Phys. (Beijing, China) 49 (2008) 268], Huang et al. gave a general variable separation solution to the (2+1)-dimensional breaking soliton equation via a special Biicldund trans... In a recent paper [Commun. Theor. Phys. (Beijing, China) 49 (2008) 268], Huang et al. gave a general variable separation solution to the (2+1)-dimensional breaking soliton equation via a special Biicldund transformation and the variable separation approach. In terms of the derived variable separation solution and by introducing Jacobi elliptic functions, they claimed that nonelastic types of interaction between Jacobi elliptic function waves are investigated both analytically and graphically. We show that some inappropriateness or errors exist in their paper, and say nothing of the conclusion that some nonelastic types of interaction between Jacobi elliptic function waves in the (2+1)-dimensional breaking soliton equation have been found. 展开更多
关键词 breaking soliton equation variable separation solution interaction COMMENT
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杏杂合位点共显性标记的分离方式及连锁图谱构建 被引量:5
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作者 章秋平 刘威生 +4 位作者 刘宁 张玉萍 刘硕 魏潇 刘有春 《园艺学报》 CAS CSCD 北大核心 2011年第10期1983-1990,共8页
以‘串枝红’ב金太阳’杏的F1代为材料,在分析共显性标记分离方式的基础上利用简单重复序列(SSR)和相关序列扩增多态性(SRAP)标记,构建了两张杏分子连锁图谱。‘串枝红’图谱共14个连锁群,包含37个SSR标记和32个SRAP标记,连锁群... 以‘串枝红’ב金太阳’杏的F1代为材料,在分析共显性标记分离方式的基础上利用简单重复序列(SSR)和相关序列扩增多态性(SRAP)标记,构建了两张杏分子连锁图谱。‘串枝红’图谱共14个连锁群,包含37个SSR标记和32个SRAP标记,连锁群总长度为1086.6cM,每连锁群平均长度77.6cM,标记间平均连锁距离为19.7cM。‘金太阳’连锁图谱共7个连锁群,包括26个SSR标记和14个SRAP标记,连锁群总长度756.7cM,每个连锁群平均长度为108.1cM,标记间平均距离为22.9cM。分析了图谱构建过程中不同共显性标记在F1代分离类型中的利用。 展开更多
关键词 杂合位点 分子标记 分离类型 连锁图谱
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Differences in Aggressiveness and Morphology of Mycosphaerella graminicola Isolates Causal Agent of Septoria Tritici Blotch on Wheat
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作者 L. Vechet E. Vydrova 《Journal of Agricultural Science and Technology(A)》 2011年第3X期386-393,共8页
Reactions 13 wheat cultivars to 18 Mycosphaerella graminicola isolates, collected from three distinct localities, were in average different but differences among individual cultivars were small. All cultivars were sus... Reactions 13 wheat cultivars to 18 Mycosphaerella graminicola isolates, collected from three distinct localities, were in average different but differences among individual cultivars were small. All cultivars were susceptible to septoria tritici blotch. Reactions of wheat cultivars to M. graminicola isolates varied from 31% to 41%. Some cultivars had low differences but the other high in reactions to used isolates in average. Variance of individual cultivars to all isolates in reactions of maximal and minimal values fluctuated between 44-80%. More differences were in reactions between individual isolates to single cultivars. Aggressiveness individual isolates to all tested cultivars oscillated in average from 9% to 66% of necrotic leaf area covered by pycnidia. Three isolates had very low, whereas five isolates had high aggressiveness in average to all cultivars. Difference in maximal and minimal aggressiveness of individual isolates to all cultivars was between 13 and 60%. Avirulent reaction was ascertained in the cultivar Rexia to one isolate. Isolates showed wide differences in colored and morphological characteristics. Dissimilarity and similarity was in border shape, cultivation types, surface and coloration of isolate colony also. 展开更多
关键词 Septoria tritici blotch AGGRESSIVENESS wheat cultivars ISOLATES morphological and color characteristics.
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Comparative Structural and Spectroscopic Characterization of M^ILn^IIITiO4 Ion-exchanged Phases,with M^I: Na, H, K
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作者 Araceli Elisabet Lavat Claudia Cecilia Wagner 《Journal of Chemistry and Chemical Engineering》 2016年第1期1-6,共6页
In this paper, a comparative study of the XRD characterization and FTIR spectroscopic behavior of some M^ILn^IIITiO4 phases with M^I: Na, H, K and Ln^III, the trivalent lanthanides elements: Sm, Eu, Er and Yb is rep... In this paper, a comparative study of the XRD characterization and FTIR spectroscopic behavior of some M^ILn^IIITiO4 phases with M^I: Na, H, K and Ln^III, the trivalent lanthanides elements: Sm, Eu, Er and Yb is reported. The H and K- oxides were obtained by ion-exchange from NaLnTiO4. These ternary oxides belong to the K2NiF4 structural type, with the M^I and Ln^III cations distributed in an ordered way on the K-sub lattice. The FTIR spectra of the materials were analyzed by comparison with structurally related materials. A close relationship with the parent NaLnTiO4 was found. 展开更多
关键词 NaLnTiO4 ion-exchange X-ray characterization FTIR spectra
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Discrete Boltzmann Method with Maxwell-Type Boundary Condition for Slip Flow 被引量:4
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作者 Yu-Dong Zhang Ai-Guo Xu +1 位作者 Guang-Cai Zhang Zhi-Hua Chen 《Communications in Theoretical Physics》 SCIE CAS CSCD 2018年第1期77-85,共9页
The rarefied effect of gas flow in microchannel is significant and cannot be well described by traditional hydrodynamic models. It has been known that discrete Boltzmann model(DBM) has the potential to investigate flo... The rarefied effect of gas flow in microchannel is significant and cannot be well described by traditional hydrodynamic models. It has been known that discrete Boltzmann model(DBM) has the potential to investigate flows in a relatively wider range of Knudsen number because of its intrinsic kinetic nature inherited from Boltzmann equation.It is crucial to have a proper kinetic boundary condition for DBM to capture the velocity slip and the flow characteristics in the Knudsen layer. In this paper, we present a DBM combined with Maxwell-type boundary condition model for slip flow. The tangential momentum accommodation coefficient is introduced to implement a gas-surface interaction model.Both the velocity slip and the Knudsen layer under various Knudsen numbers and accommodation coefficients can be well described. Two kinds of slip flows, including Couette flow and Poiseuille flow, are simulated to verify the model.To dynamically compare results from different models, the relation between the definition of Knudsen number in hard sphere model and that in BGK model is clarified. 展开更多
关键词 rarefied gas discrete Boltzmann method Maxwell-type boundary slip flow
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New Similarity Reduction Solutions for the(2+1)-Dimensional Nizhnik-Novikov-Veselov Equation 被引量:1
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作者 智红燕 《Communications in Theoretical Physics》 SCIE CAS CSCD 2013年第3期263-267,共5页
In this paper,some new formal similarity reduction solutions for the(2+1)-dimensional Nizhnik-Novikov-Veselov equation are derived.Firstly,we derive the similarity reduction of the NNV equation with the optimal system... In this paper,some new formal similarity reduction solutions for the(2+1)-dimensional Nizhnik-Novikov-Veselov equation are derived.Firstly,we derive the similarity reduction of the NNV equation with the optimal system of the admitted one-dimensional subalgebras.Secondly,by analyzing the reduced equation,three types of similarity solutions are derived,such as multi-soliton like solutions,variable separations solutions,and KdV type solutions. 展开更多
关键词 similarity reduction solution (2+1)-dimensional Nizknik-Novikov-Veselov equation multi-solitonlike solution
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Conditional Lie-Bcklund Symmetry and New Variable Separation Solutions of the Third Order KdV-Type Equations 被引量:1
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作者 Gai-Zhu Qu Shun-Li Zhang +1 位作者 Hai-Xia Li Gang-Wei Wang 《Communications in Theoretical Physics》 SCIE CAS CSCD 2018年第10期399-404,共6页
The functionally generalized variable separation solutions of a general KdV-type equations u_t=u_(xxx) +A(u, u_x)u_(xx) + B(u, u_x) are investigated by developing the conditional Lie-Backlund symmetry method. A comple... The functionally generalized variable separation solutions of a general KdV-type equations u_t=u_(xxx) +A(u, u_x)u_(xx) + B(u, u_x) are investigated by developing the conditional Lie-Backlund symmetry method. A complete classification of the considered equations, which admit multi-dimensional invariant subspaces governed by higher-order conditional Lie-B¨acklund symmetries, is presented. As a result, several concrete examples are provided to construct functionally generalized variable separation solutions of some resulting equations. 展开更多
关键词 KdV-type equation conditional Lie-Backlund symmetry invariant subspace functionally gener-alized separation solutions dynamical system
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Examples of Boundary Layers Associated with the Incompressible Navier-Stokes Equations
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作者 Xiaoming WANG 《Chinese Annals of Mathematics,Series B》 SCIE CSCD 2010年第5期781-792,共12页
The author surveys a few examples of boundary layers for which the Prandtl boundary layer theory can be rigorously validated.All of them are associated with the incompressible Navier-Stokes equations for Newtonian flu... The author surveys a few examples of boundary layers for which the Prandtl boundary layer theory can be rigorously validated.All of them are associated with the incompressible Navier-Stokes equations for Newtonian fluids equipped with various Dirichlet boundary conditions(specified velocity).These examples include a family of(nonlinear 3D) plane parallel flows,a family of(nonlinear) parallel pipe flows,as well as flows with uniform injection and suction at the boundary.We also identify a key ingredient in establishing the validity of the Prandtl type theory,i.e.,a spectral constraint on the approximate solution to the Navier-Stokes system constructed by combining the inviscid solution and the solution to the Prandtl type system.This is an additional difficulty besides the wellknown issue related to the well-posedness of the Prandtl type system.It seems that the main obstruction to the verification of the spectral constraint condition is the possible separation of boundary layers.A common theme of these examples is the inhibition of separation of boundary layers either via suppressing the velocity normal to the boundary or by injection and suction at the boundary so that the spectral constraint can be verified.A meta theorem is then presented which covers all the cases considered here. 展开更多
关键词 Boundary layer Navier-Stokes system Prandtl theory CORRECTOR Inviscid limit Spectral constraint Nonlinear plane parallel channel flow Nonlinear pipe flow Injection and suction
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