Hydrophilic surface modification of polysulfone hollow fiber membrane was performed by acrylic acid treatment.Stannic chloride was selected as the proper catalyst, and phosphoric acid as the co-catalyst.The modificati...Hydrophilic surface modification of polysulfone hollow fiber membrane was performed by acrylic acid treatment.Stannic chloride was selected as the proper catalyst, and phosphoric acid as the co-catalyst.The modification conditions, such as the ratio of the reagents (acrylic acid∶catalyst=1∶0.05), temperature (30℃) and reaction time (60 min), were optimized based on the contact angle measurements.The grafting of carboxyl group to the membrane surface was confirmed by IR spectrum and wettability analysis, and no degradation of the modified membrane skin or framework was observed with scanning electron microscopy.The experimental results of filtrating bovine serum albumin solutions indicated that the property of resisting protein adsorption on the membrane surface for the modified membrane was much better than that for unmodified membrane.In the process of separating casein hydrolysates, the advantages of modified membrane were also shown by comparing the permeate flux and molecular mass distribution with those of unmodified membrane.展开更多
采用DEAE-FF离子交换和Sephdax G-15葡聚糖凝胶层析方法,从玉米肽中分离、纯化,获得具有抑制HepG2细胞增殖活性的短肽,采用高效液相色谱串联质谱电喷雾法(liquid chromatography-electrospray ionization tandem mass spectrometry,LC-E...采用DEAE-FF离子交换和Sephdax G-15葡聚糖凝胶层析方法,从玉米肽中分离、纯化,获得具有抑制HepG2细胞增殖活性的短肽,采用高效液相色谱串联质谱电喷雾法(liquid chromatography-electrospray ionization tandem mass spectrometry,LC-ESI-MS/MS)对其活性片段进行结构鉴定。结果表明:其短肽的一级结构为LPPYLP[命名为玉米六肽(corn peptides-6,CPs-6)],通过四唑盐[3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,MTT]比色法试验发现,CPs-6具有抑制HepG2细胞增殖的效果,且对L-02细胞的毒性低。展开更多
文摘Hydrophilic surface modification of polysulfone hollow fiber membrane was performed by acrylic acid treatment.Stannic chloride was selected as the proper catalyst, and phosphoric acid as the co-catalyst.The modification conditions, such as the ratio of the reagents (acrylic acid∶catalyst=1∶0.05), temperature (30℃) and reaction time (60 min), were optimized based on the contact angle measurements.The grafting of carboxyl group to the membrane surface was confirmed by IR spectrum and wettability analysis, and no degradation of the modified membrane skin or framework was observed with scanning electron microscopy.The experimental results of filtrating bovine serum albumin solutions indicated that the property of resisting protein adsorption on the membrane surface for the modified membrane was much better than that for unmodified membrane.In the process of separating casein hydrolysates, the advantages of modified membrane were also shown by comparing the permeate flux and molecular mass distribution with those of unmodified membrane.
文摘采用DEAE-FF离子交换和Sephdax G-15葡聚糖凝胶层析方法,从玉米肽中分离、纯化,获得具有抑制HepG2细胞增殖活性的短肽,采用高效液相色谱串联质谱电喷雾法(liquid chromatography-electrospray ionization tandem mass spectrometry,LC-ESI-MS/MS)对其活性片段进行结构鉴定。结果表明:其短肽的一级结构为LPPYLP[命名为玉米六肽(corn peptides-6,CPs-6)],通过四唑盐[3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,MTT]比色法试验发现,CPs-6具有抑制HepG2细胞增殖的效果,且对L-02细胞的毒性低。
