In our work, a new extraction tip with gold-modified polymer is developed. The simple, self-made and extremely economical tips were successfully applied to capture cysteine-containing peptides. The loading capacity of...In our work, a new extraction tip with gold-modified polymer is developed. The simple, self-made and extremely economical tips were successfully applied to capture cysteine-containing peptides. The loading capacity of a tip （column bed： 0.3 mm di- ameter, 5 mm length） is 2-4 lag peptides. We can make one tip in 30 s and each costs less than 0.1 cent. The use of these tips can achieve a stable analysis with less background interference, even for 10 ng target peptides. Compared with other separation techniques, our method can save much time and energy while providing a means to selectively capture cysteine-containing peptides from complex analyte due to the strong interaction. All results showed that our new extraction tips have minimal cost and perfect selectivity; thus they have great potential in sample pretreatment systems for proteomics.展开更多
A closed orientable Haken 3-manifold containing a non separating incompressible closed surface has two canonical Heegaard splittings, which are called self-amalgamation and bilateral self-amalgamation.Heegaard distanc...A closed orientable Haken 3-manifold containing a non separating incompressible closed surface has two canonical Heegaard splittings, which are called self-amalgamation and bilateral self-amalgamation.Heegaard distance introduced by Hempel is a useful index in studying Heegaard splitting. This paper studies the stabilization problem for the bilateral self-amalgamation, and proves that if the distance of bilateral selfamalgamation of a Heegaard splitting is at least 9, then it is unstabilized, weakly reducible and irreducible.展开更多
A coupled discrete-continuum simulation incorporating a 3D aspect and non-circular particles was performed to analyze soil-pile interactions during pile penetration in sand.A self-developed non-circular particle numer...A coupled discrete-continuum simulation incorporating a 3D aspect and non-circular particles was performed to analyze soil-pile interactions during pile penetration in sand.A self-developed non-circular particle numerical simulation program was used which considered sand near the pile as interacted particles using a discrete element method;the sand away from the pile was simulated as a continuous medium exhibiting linear elastic behaviors.The domain analyzed was divided into two zones.Contact forces at the interface between the two zones were obtained from a discrete zone and applied to the continuum boundaries as nodal forces,while the interface velocities were obtained from the continuum zone and applied to the discrete boundaries.We show that the coupled discrete-continuum simulation can give a microscopic description of the pile penetration process without losing the discrete nature of the zone concerned,and may significantly improve computational efficiency.展开更多
基金financially supported by the National High-Tech R&D Program(2012AA020202)the National Basic Research Program of China(2012CB910604)the National Natural Science Foundation of China(21275034,21175026)
文摘In our work, a new extraction tip with gold-modified polymer is developed. The simple, self-made and extremely economical tips were successfully applied to capture cysteine-containing peptides. The loading capacity of a tip （column bed： 0.3 mm di- ameter, 5 mm length） is 2-4 lag peptides. We can make one tip in 30 s and each costs less than 0.1 cent. The use of these tips can achieve a stable analysis with less background interference, even for 10 ng target peptides. Compared with other separation techniques, our method can save much time and energy while providing a means to selectively capture cysteine-containing peptides from complex analyte due to the strong interaction. All results showed that our new extraction tips have minimal cost and perfect selectivity; thus they have great potential in sample pretreatment systems for proteomics.
基金supported by National Natural Science Foundation of China(Grant Nos.11271058 and 11371076)the Fundamental Research Funds for the Central Universities(Grant No.DUT14ZD208)
文摘A closed orientable Haken 3-manifold containing a non separating incompressible closed surface has two canonical Heegaard splittings, which are called self-amalgamation and bilateral self-amalgamation.Heegaard distance introduced by Hempel is a useful index in studying Heegaard splitting. This paper studies the stabilization problem for the bilateral self-amalgamation, and proves that if the distance of bilateral selfamalgamation of a Heegaard splitting is at least 9, then it is unstabilized, weakly reducible and irreducible.
基金Project (No.90815008) supported by the National Natural Science Foundation of China
文摘A coupled discrete-continuum simulation incorporating a 3D aspect and non-circular particles was performed to analyze soil-pile interactions during pile penetration in sand.A self-developed non-circular particle numerical simulation program was used which considered sand near the pile as interacted particles using a discrete element method;the sand away from the pile was simulated as a continuous medium exhibiting linear elastic behaviors.The domain analyzed was divided into two zones.Contact forces at the interface between the two zones were obtained from a discrete zone and applied to the continuum boundaries as nodal forces,while the interface velocities were obtained from the continuum zone and applied to the discrete boundaries.We show that the coupled discrete-continuum simulation can give a microscopic description of the pile penetration process without losing the discrete nature of the zone concerned,and may significantly improve computational efficiency.
