Recent advances in the evolutionary genetics of sex determination indicate that the only molecular similarity in sex determination found so far among phyla is between the fly doublesex, worm mab-3 and vertebrate DMRTI...Recent advances in the evolutionary genetics of sex determination indicate that the only molecular similarity in sex determination found so far among phyla is between the fly doublesex, worm mab-3 and vertebrate DMRTI（dsx- and mab3-related transcription factor 1）/DMY genes. Each of these factors encodes a zinc-finger-like DNA-binding motif, DM domain. Insights into the evolution and functions of human DMRT1 gene could reveal evolutionary mechanisms of sexual development. Here we report the identification and characterization of multiple isoforms of human DMRT1 in the testis. These transcripts encode predicted proteins with 373,275 and 175 amino acids and they were generated by alternative splicing at 3＇ region. Expression level of DMRTla is higher than those of both DMRTlb and c, and the DMR Tlc expression was the lowest in testis, based on comparisons of mean values from real-time fluorescent quantitative RT-PCR analysis. Both DMRTlb and c result from exonization of intronic sequences, including the exonization of an Alu element. A further search for Alu elements within the DMRT1 gene demonstrated that all 99 Alu elements are non-randomly distributed among the non-coding regions on both directions. These new characteristics of DMRT1 would have an important impact on the evolution of sexual development mechanisms.展开更多
Water exploits an essential part in all the durable development, the exploitation of this natural resource, its management and its position, its mineralization, its underground course and the mode o fits resurgence. T...Water exploits an essential part in all the durable development, the exploitation of this natural resource, its management and its position, its mineralization, its underground course and the mode o fits resurgence. The area of Gafsa is known by its very important hydrous potential which is geographically in the zone of transition between central Tunisia and the Saharan platform, these two great fields are separated by the fault of Gafsa which plays an important part in the structuring of the basins of the area, the hydrous resources of Gafsa make subsoil water the principal source of supply water, in the absence of an important network of surface. The zones of studies present requirements out of water which do not cease growing with the progress of the industrial activities and the concentration of the agricultural activities： The objective of this work is to analyze the water of the various zones of the area of Gafsa and to treat it to make it possible most drinkable. The analyzes like measurement of pH, measurement of electric conductivity and measurement of dry residue were made in the laboratory of the chemical group of Mdhila in Tunisia and in laboratory of Ion exchanger Materials of Vitry in France, the processes of treatment as opposite osmosis are also programmed in this same laboratory. The results are very encouraging to start the study and the design of a station of potabilisation of water in the area of Gafsa.展开更多
OBJECTIVE:To observe the effects of different doses of Wenxiao Ⅱ Decoction on the expression of monocyte chemoattractant protein-1(MCP-1) and vascular cell adhesion molecule-1(VCAM-1) in an experimental model of athe...OBJECTIVE:To observe the effects of different doses of Wenxiao Ⅱ Decoction on the expression of monocyte chemoattractant protein-1(MCP-1) and vascular cell adhesion molecule-1(VCAM-1) in an experimental model of atherosclerosis in rabbits and to explore the mechanism by which it alleviates atherosclerosis.METHODS:Sixty 3-4 month-old New Zealand rabbits of both sexes were randomly divided into six groups:simvastain;model;blank;and high-dose,mid-dose,and low-dose Wenxiao Ⅱ Decoction groups.Except for those in the blank group,all rabbits were fed a high-cholesterol diet.Carotid atherosclerosis was established by balloon-induced injury to the endothelium of the carotid artery in conjunction with consumption of a high-cholesterol diet.After 8 weeks,all rabbits were killed to evaluate the expression of MCP-1 and VCAM-1 by immunohistochemical staining.RESULTS:Expressions of MCP-1 and VCAM-1 were significantly decreased in all groups except the blank group compared with the model group(P<0.05).When compared with the simvastain group only variation of MCP-1 expression in low-dose group was not appreciable,and the differences were indistinct(P<0.05).When comparing among Wenxiao Ⅱ Decoction groups,MCP-1 expression in the mid-and high-dose groups was significantly lower than that seen in the low-dose group(P<0.01),but there were no differences among three dosage groups with respect to VCAM-1 expression(P>0.05).CONCLUSION:These data suggested that high,mid,and low doses of Wenxiao Ⅱ Decoction can inhibit the expression of MCP-1 and VCAM-1,which may prevent the formation of or stabilize atherosclerotic plaques.There may be a direct relationship between the dosage of Wenxiao Ⅱ Decoction and its therapeutic efficacy.展开更多
基金The work was supported by the National Natural Science Foundation of China, and the National Key Basic Research project （2004CB117400）the Program for New Century Excellent Talents in University and the Key Project of Chinese Ministry of Education （No. 2004.28）.
文摘Recent advances in the evolutionary genetics of sex determination indicate that the only molecular similarity in sex determination found so far among phyla is between the fly doublesex, worm mab-3 and vertebrate DMRTI（dsx- and mab3-related transcription factor 1）/DMY genes. Each of these factors encodes a zinc-finger-like DNA-binding motif, DM domain. Insights into the evolution and functions of human DMRT1 gene could reveal evolutionary mechanisms of sexual development. Here we report the identification and characterization of multiple isoforms of human DMRT1 in the testis. These transcripts encode predicted proteins with 373,275 and 175 amino acids and they were generated by alternative splicing at 3＇ region. Expression level of DMRTla is higher than those of both DMRTlb and c, and the DMR Tlc expression was the lowest in testis, based on comparisons of mean values from real-time fluorescent quantitative RT-PCR analysis. Both DMRTlb and c result from exonization of intronic sequences, including the exonization of an Alu element. A further search for Alu elements within the DMRT1 gene demonstrated that all 99 Alu elements are non-randomly distributed among the non-coding regions on both directions. These new characteristics of DMRT1 would have an important impact on the evolution of sexual development mechanisms.
文摘Water exploits an essential part in all the durable development, the exploitation of this natural resource, its management and its position, its mineralization, its underground course and the mode o fits resurgence. The area of Gafsa is known by its very important hydrous potential which is geographically in the zone of transition between central Tunisia and the Saharan platform, these two great fields are separated by the fault of Gafsa which plays an important part in the structuring of the basins of the area, the hydrous resources of Gafsa make subsoil water the principal source of supply water, in the absence of an important network of surface. The zones of studies present requirements out of water which do not cease growing with the progress of the industrial activities and the concentration of the agricultural activities： The objective of this work is to analyze the water of the various zones of the area of Gafsa and to treat it to make it possible most drinkable. The analyzes like measurement of pH, measurement of electric conductivity and measurement of dry residue were made in the laboratory of the chemical group of Mdhila in Tunisia and in laboratory of Ion exchanger Materials of Vitry in France, the processes of treatment as opposite osmosis are also programmed in this same laboratory. The results are very encouraging to start the study and the design of a station of potabilisation of water in the area of Gafsa.
基金Supported by Research Grant from the Health Bureau of Shanghai(No.2008J003A)
文摘OBJECTIVE:To observe the effects of different doses of Wenxiao Ⅱ Decoction on the expression of monocyte chemoattractant protein-1(MCP-1) and vascular cell adhesion molecule-1(VCAM-1) in an experimental model of atherosclerosis in rabbits and to explore the mechanism by which it alleviates atherosclerosis.METHODS:Sixty 3-4 month-old New Zealand rabbits of both sexes were randomly divided into six groups:simvastain;model;blank;and high-dose,mid-dose,and low-dose Wenxiao Ⅱ Decoction groups.Except for those in the blank group,all rabbits were fed a high-cholesterol diet.Carotid atherosclerosis was established by balloon-induced injury to the endothelium of the carotid artery in conjunction with consumption of a high-cholesterol diet.After 8 weeks,all rabbits were killed to evaluate the expression of MCP-1 and VCAM-1 by immunohistochemical staining.RESULTS:Expressions of MCP-1 and VCAM-1 were significantly decreased in all groups except the blank group compared with the model group(P<0.05).When compared with the simvastain group only variation of MCP-1 expression in low-dose group was not appreciable,and the differences were indistinct(P<0.05).When comparing among Wenxiao Ⅱ Decoction groups,MCP-1 expression in the mid-and high-dose groups was significantly lower than that seen in the low-dose group(P<0.01),but there were no differences among three dosage groups with respect to VCAM-1 expression(P>0.05).CONCLUSION:These data suggested that high,mid,and low doses of Wenxiao Ⅱ Decoction can inhibit the expression of MCP-1 and VCAM-1,which may prevent the formation of or stabilize atherosclerotic plaques.There may be a direct relationship between the dosage of Wenxiao Ⅱ Decoction and its therapeutic efficacy.
