[Objective] This study aimed to establish an efficient process for separation of phycoerythrin by using Q Sepharose Fast Flow resin and verity its feasibility for scale-up. [Method] Elution gradient, sample volume and...[Objective] This study aimed to establish an efficient process for separation of phycoerythrin by using Q Sepharose Fast Flow resin and verity its feasibility for scale-up. [Method] Elution gradient, sample volume and flow rate were optimized to determine the optimal separation condition, under which the scale-up process was verified. [Result] The optimal condition for separation of phycoerythrin by using Q Sepharose FF resin was investigated： 30 ml of laver extract was loaded to the Q Sepharose FF column with a bed volume of 8 ml; subsequently, the column was stepwise eluted with 0-0.10-0.35-1.00 mol/L NaCI solution （pH 6.0） at a constant flow rate of 1 ml/min; the elution peak under 0.35 mol/L NaCI solution was collected, and the recovery rate and purity coefficient （A565/A280） of phycoerythrin were determined as 44.3 and 1.15, respectively. Based on the established process, 75 ml of phycoerythrin extract was loaded to the Q Sepharose FF column with a bed volume of 20 ml for separation, while no significant variation was observed in the separation result. [Conclusion] Phycoerythrin can be well separated from laver extract by using Q Sepharose FF resin and the process is feasible for scale-up.展开更多
The soybean fiber was treated with low temperature plasma and the dyeing performance of the treated soybean fiber was also researched. The results show that the speed of dyeing and the percentages of balance dyeing ha...The soybean fiber was treated with low temperature plasma and the dyeing performance of the treated soybean fiber was also researched. The results show that the speed of dyeing and the percentages of balance dyeing have a sharp increase after being treated. So the dyeing temperature and the dosage of acid can be reduced without damaging the bulk fiber structure.展开更多
Changes of dyeing behavior of wool after stretching - setting treatment are studied.Effects of stretching - setting on wool structure are analyzed.It is concluded that stretching - setting treatment not only reduces w...Changes of dyeing behavior of wool after stretching - setting treatment are studied.Effects of stretching - setting on wool structure are analyzed.It is concluded that stretching - setting treatment not only reduces wool diameter and increases wool length,but also brings about low-temperature dyeing of wool.展开更多
In order to study the influence of wood microstructure on wood dyeing, eleven parameters of wood microstructure and 5 parameters of wood dyeing effects for 34 pieces of wood boards from 5 trees of Chinese white poplar...In order to study the influence of wood microstructure on wood dyeing, eleven parameters of wood microstructure and 5 parameters of wood dyeing effects for 34 pieces of wood boards from 5 trees of Chinese white poplar (Populus tomentosa) were determined and the multiple regression analysis between the factors of wood microstructures and the parameters of wood dyeing effects were made. The regression results show that each variable of wood dyeing effects has higher relationship with wood microstructures, and multiple correlation coefficients between each variable of wood dyeing effects and wood microstructures are 0.483 6~0.799 8. The main factors of wood microstructures influencing wood dyeing of Chinese white poplar are proportion of wood ray, proportion of vessel and proportion of wood fiber according to comparing the standardized regression coefficients of multiple regression equation.展开更多
基金Supported by National Natural Science Foundation of China(51143012)Natural Science Foundation of Shandong Province(ZR2009BM006)~~
文摘[Objective] This study aimed to establish an efficient process for separation of phycoerythrin by using Q Sepharose Fast Flow resin and verity its feasibility for scale-up. [Method] Elution gradient, sample volume and flow rate were optimized to determine the optimal separation condition, under which the scale-up process was verified. [Result] The optimal condition for separation of phycoerythrin by using Q Sepharose FF resin was investigated： 30 ml of laver extract was loaded to the Q Sepharose FF column with a bed volume of 8 ml; subsequently, the column was stepwise eluted with 0-0.10-0.35-1.00 mol/L NaCI solution （pH 6.0） at a constant flow rate of 1 ml/min; the elution peak under 0.35 mol/L NaCI solution was collected, and the recovery rate and purity coefficient （A565/A280） of phycoerythrin were determined as 44.3 and 1.15, respectively. Based on the established process, 75 ml of phycoerythrin extract was loaded to the Q Sepharose FF column with a bed volume of 20 ml for separation, while no significant variation was observed in the separation result. [Conclusion] Phycoerythrin can be well separated from laver extract by using Q Sepharose FF resin and the process is feasible for scale-up.
基金Supported by Shanghai Leading Academic Discipline Project (No.P1404)
文摘The soybean fiber was treated with low temperature plasma and the dyeing performance of the treated soybean fiber was also researched. The results show that the speed of dyeing and the percentages of balance dyeing have a sharp increase after being treated. So the dyeing temperature and the dosage of acid can be reduced without damaging the bulk fiber structure.
文摘Changes of dyeing behavior of wool after stretching - setting treatment are studied.Effects of stretching - setting on wool structure are analyzed.It is concluded that stretching - setting treatment not only reduces wool diameter and increases wool length,but also brings about low-temperature dyeing of wool.
文摘In order to study the influence of wood microstructure on wood dyeing, eleven parameters of wood microstructure and 5 parameters of wood dyeing effects for 34 pieces of wood boards from 5 trees of Chinese white poplar (Populus tomentosa) were determined and the multiple regression analysis between the factors of wood microstructures and the parameters of wood dyeing effects were made. The regression results show that each variable of wood dyeing effects has higher relationship with wood microstructures, and multiple correlation coefficients between each variable of wood dyeing effects and wood microstructures are 0.483 6~0.799 8. The main factors of wood microstructures influencing wood dyeing of Chinese white poplar are proportion of wood ray, proportion of vessel and proportion of wood fiber according to comparing the standardized regression coefficients of multiple regression equation.
