Bacillus subtilis strain C19, capable of ulilizing pyrene as a sole of carbon and energy, was isolated from marine Indonesian Archipelago. In biodegradation of pyrene by B. subtilis C19, various metabolites was isolat...Bacillus subtilis strain C19, capable of ulilizing pyrene as a sole of carbon and energy, was isolated from marine Indonesian Archipelago. In biodegradation of pyrene by B. subtilis C19, various metabolites was isolated and identified by chromatographic and spectral analyses. Lipopeptide biosurfactant was produced and it has stable emulsification activity. Biosurfactant was produced for enhancing pyrene uptake and bioavaibility. After 30 days incubation, there were no toxic metabolite and biodegradation of pyrene was already complete (only 0.02% undegradable metabolite). Therefore, this strain is suitable for PAHs (polyaromatic hydrocarbons) contaminated environment recovery.展开更多
T4 lysozyme was engineered with disulfide bonds and expressed in Pichia pastoris. The secreted proteins were purified and made into powder by lyophiliza-tion. Recombinant protein purity was more than 70% measured by H...T4 lysozyme was engineered with disulfide bonds and expressed in Pichia pastoris. The secreted proteins were purified and made into powder by lyophiliza-tion. Recombinant protein purity was more than 70% measured by HPLC. The lytic activity of variant T4-lysozyme was measured by the lysis of the cel wal of Xan-thomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, Ralstonia solanacearum comb. nov, Clavibacter michiganensis subsp. michiganensis, X. campestris pv. mal-vacearum, Fusarium oxysporium sp. vasinfectum, Verticil ium dahliae kleb. Inhibition zone assay showed that variant T4 lysozyme significantly inhibited X. o. oryzicola and X. c. malvacearum. The antifungal activities of this protein against F. o. vasin-fectum and V. d. kleb were also analyzed.展开更多
We present a high-resolution relaxation scheme for a multi-class Lighthill-Whitham-Richards (MCLWR) traffic flow model. This scheme is based on high-order reconstruction for spatial discretization and an implicit-expl...We present a high-resolution relaxation scheme for a multi-class Lighthill-Whitham-Richards (MCLWR) traffic flow model. This scheme is based on high-order reconstruction for spatial discretization and an implicit-explicit Runge-Kutta method for time integration. The resulting method retains the simplicity of the relaxation schemes. There is no need to involve Riemann solvers and characteristic decomposition. Even the computation of the eigenvalues is not required. This makes the scheme particularly well suited for the MCLWR model in which the analytical expressions of the eigenvalues are difficult to obtain for more than four classes of road users. The numerical results illustrate the effectiveness of the presented method.展开更多
Photoelectorchemical(PEC) water splitting is an attractive approach for producing sustainable and environment-friendly hydrogen. An efficient PEC process is rooted in appropriate semiconductor materials, which shoul...Photoelectorchemical(PEC) water splitting is an attractive approach for producing sustainable and environment-friendly hydrogen. An efficient PEC process is rooted in appropriate semiconductor materials, which should possess small bandgap to ensure wide light harvest, facile charge separation to allow the generated photocharges migrating to the reactive sites and highly catalytic capability to fully utilize the separated photocharges. Proper electrode fabrication method is of equal importance for promoting charge transfer and accelerating surface reactions in the electrodes. Moreover,powerful characterization method can shed light on the complex PEC process and provide deep understanding of the rate-determining step for us to improve the PEC systems further. Targeting on high solar conversion efficiency, here we provide a review on the development of PEC water splitting in the aspect of materials exploring, fabrication method and characterization. It is expected to provide some fundamental insight of PEC and inspire the design of more effective PEC systems.展开更多
文摘Bacillus subtilis strain C19, capable of ulilizing pyrene as a sole of carbon and energy, was isolated from marine Indonesian Archipelago. In biodegradation of pyrene by B. subtilis C19, various metabolites was isolated and identified by chromatographic and spectral analyses. Lipopeptide biosurfactant was produced and it has stable emulsification activity. Biosurfactant was produced for enhancing pyrene uptake and bioavaibility. After 30 days incubation, there were no toxic metabolite and biodegradation of pyrene was already complete (only 0.02% undegradable metabolite). Therefore, this strain is suitable for PAHs (polyaromatic hydrocarbons) contaminated environment recovery.
基金Supported by the National Biotechnology Program for Crop Breeding(2013ZX08005004,2009ZX08009-089B)
文摘T4 lysozyme was engineered with disulfide bonds and expressed in Pichia pastoris. The secreted proteins were purified and made into powder by lyophiliza-tion. Recombinant protein purity was more than 70% measured by HPLC. The lytic activity of variant T4-lysozyme was measured by the lysis of the cel wal of Xan-thomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, Ralstonia solanacearum comb. nov, Clavibacter michiganensis subsp. michiganensis, X. campestris pv. mal-vacearum, Fusarium oxysporium sp. vasinfectum, Verticil ium dahliae kleb. Inhibition zone assay showed that variant T4 lysozyme significantly inhibited X. o. oryzicola and X. c. malvacearum. The antifungal activities of this protein against F. o. vasin-fectum and V. d. kleb were also analyzed.
基金Project supported by the Aoxiang Project and the Scientific and Technological Innovation Foundation of Northwestern Polytechnical University, China (No 2007KJ01011)
文摘We present a high-resolution relaxation scheme for a multi-class Lighthill-Whitham-Richards (MCLWR) traffic flow model. This scheme is based on high-order reconstruction for spatial discretization and an implicit-explicit Runge-Kutta method for time integration. The resulting method retains the simplicity of the relaxation schemes. There is no need to involve Riemann solvers and characteristic decomposition. Even the computation of the eigenvalues is not required. This makes the scheme particularly well suited for the MCLWR model in which the analytical expressions of the eigenvalues are difficult to obtain for more than four classes of road users. The numerical results illustrate the effectiveness of the presented method.
基金supported by the Australian Research Council through its Discovery Project (DP)Federation Fellowship (FF) Program
文摘Photoelectorchemical(PEC) water splitting is an attractive approach for producing sustainable and environment-friendly hydrogen. An efficient PEC process is rooted in appropriate semiconductor materials, which should possess small bandgap to ensure wide light harvest, facile charge separation to allow the generated photocharges migrating to the reactive sites and highly catalytic capability to fully utilize the separated photocharges. Proper electrode fabrication method is of equal importance for promoting charge transfer and accelerating surface reactions in the electrodes. Moreover,powerful characterization method can shed light on the complex PEC process and provide deep understanding of the rate-determining step for us to improve the PEC systems further. Targeting on high solar conversion efficiency, here we provide a review on the development of PEC water splitting in the aspect of materials exploring, fabrication method and characterization. It is expected to provide some fundamental insight of PEC and inspire the design of more effective PEC systems.