Mangrove （Sonneratia spp.） could be found at Waai seashore, Ambon island. The remainder of the mangrove stem will be decayed and become the waste product. Some indigenous bacteria species that live in the decayed ma...Mangrove （Sonneratia spp.） could be found at Waai seashore, Ambon island. The remainder of the mangrove stem will be decayed and become the waste product. Some indigenous bacteria species that live in the decayed mangrove stem waste product have cellulolytic and amylolytic characters. This research was done to： （1） identify and determine the cellulolytic bacteria species; （2） identify and determine the amylolytic bacteria species; （3） determine the cellulolytic bacteria species that have the highest cellulase activity; （4） determine the amylolytic bacteria species that have the highest amylase activity. This research was conducted at the Microbiology Laboratory, Department of Biology, State University of Malang, Microbiology Laboratory, Faculty of Medicine, Brawijaya University and Chemistry Laboratory, Muhammadiyah Malang University. In the experiment, 25 g sample were grinded and diluted in 225 mL nutrient broth to get 101 suspension. Then the suspension was diluted gradually until 101~. The suspension was inoculated on nutrient agar medium with 0.1 mL each, and incubated in 37 ~C during 24 h. Each bacteria colony was isolated and identified to know whether it was cellulolytic or amylolytic bacteria. Afterward, the cellulase activity as well as the amylase activity was analyzed. The research results show that： （1） there are four cellulolytic bacteria species, i.e., Micrococcus luteus, Bacillus pumilus, Planococcus citreus and Bacillus cereus; （2） there are four amylolytic bacteria species, i.e., Bacillus firmus, Nitrobacter sp., Bacillus mycoides and Pseudomonas aeruginosa; （3） Bacillus cereus has the highest cellulase activity; （4） Nitrobacter sp. has the highest amylase activity.展开更多
In the present study, it has been aimed to determine the non target effects of Congo red on soil enzyme activities namely amylase, protease, catalase, glucose oxidase and laccases, following the standard procedures. F...In the present study, it has been aimed to determine the non target effects of Congo red on soil enzyme activities namely amylase, protease, catalase, glucose oxidase and laccases, following the standard procedures. Further, it is also aimed to decolourize the dye effluents by using immobilized fimgal cultures namely Aspergillus spp. isolated from textile effluents and estimate the percentage of decolourization by dye decolourization assay method. The selected fungi were found efficient and dominant type in dye decolourization with the production of laccase and other enzymes. Except laccase, all the enzymes showed maximum activity at 14 days followed by declining trend at 21 days. Maximum enzyme activity was noticed with Aspergillus niger. 95% of decolourization was found within 4-24 h in sodium alginate immobilized fungal cultures compared to controls. The above results clearly indicate the efficiency of these fungi in selective bioremediation of dye contaminated sites.展开更多
文摘Mangrove （Sonneratia spp.） could be found at Waai seashore, Ambon island. The remainder of the mangrove stem will be decayed and become the waste product. Some indigenous bacteria species that live in the decayed mangrove stem waste product have cellulolytic and amylolytic characters. This research was done to： （1） identify and determine the cellulolytic bacteria species; （2） identify and determine the amylolytic bacteria species; （3） determine the cellulolytic bacteria species that have the highest cellulase activity; （4） determine the amylolytic bacteria species that have the highest amylase activity. This research was conducted at the Microbiology Laboratory, Department of Biology, State University of Malang, Microbiology Laboratory, Faculty of Medicine, Brawijaya University and Chemistry Laboratory, Muhammadiyah Malang University. In the experiment, 25 g sample were grinded and diluted in 225 mL nutrient broth to get 101 suspension. Then the suspension was diluted gradually until 101~. The suspension was inoculated on nutrient agar medium with 0.1 mL each, and incubated in 37 ~C during 24 h. Each bacteria colony was isolated and identified to know whether it was cellulolytic or amylolytic bacteria. Afterward, the cellulase activity as well as the amylase activity was analyzed. The research results show that： （1） there are four cellulolytic bacteria species, i.e., Micrococcus luteus, Bacillus pumilus, Planococcus citreus and Bacillus cereus; （2） there are four amylolytic bacteria species, i.e., Bacillus firmus, Nitrobacter sp., Bacillus mycoides and Pseudomonas aeruginosa; （3） Bacillus cereus has the highest cellulase activity; （4） Nitrobacter sp. has the highest amylase activity.
文摘In the present study, it has been aimed to determine the non target effects of Congo red on soil enzyme activities namely amylase, protease, catalase, glucose oxidase and laccases, following the standard procedures. Further, it is also aimed to decolourize the dye effluents by using immobilized fimgal cultures namely Aspergillus spp. isolated from textile effluents and estimate the percentage of decolourization by dye decolourization assay method. The selected fungi were found efficient and dominant type in dye decolourization with the production of laccase and other enzymes. Except laccase, all the enzymes showed maximum activity at 14 days followed by declining trend at 21 days. Maximum enzyme activity was noticed with Aspergillus niger. 95% of decolourization was found within 4-24 h in sodium alginate immobilized fungal cultures compared to controls. The above results clearly indicate the efficiency of these fungi in selective bioremediation of dye contaminated sites.
