The RNA helicase Vasa is an important regulator of primordial germ cell development. Its function in mature fish, espe- cially the hormone-related differences in maturing male fish has seldom been documented. In this ...The RNA helicase Vasa is an important regulator of primordial germ cell development. Its function in mature fish, espe- cially the hormone-related differences in maturing male fish has seldom been documented. In this study, a full length cDNA sequence of the vasa gene was cloned from Japanese sea bass, Lateolabraxjaponicas, and it was namedjsb-vasa. Homology analysis showed thatjsb-vasa was closely related to its teleost homologs. The spatial distribution ofjsb-vasa indicated that it was only highly ex- pressed in testis, showing its germ cell-specific expression pattern. During the testicular development cycle, jsb-vasa was highly expressed during early period of spermatogenesis, and reduced when spermatogenesis advanced. In addition, the jsb-vasa gene ex- pression was significantly inhibited at 6 h, 12 h and 24 h after injecting hCG （human ehorionic gonadotropin） and GnRHa （Gonad- otropin-releasing hormone analogue）, indicating thatjsb-vasa gene may play an important role in spermatogenesis of Japanese sea bass, and be under the regulation of external sex hormones.展开更多
Mangrove （Sonneratia spp.） could be found at Waai seashore, Ambon island. The remainder of the mangrove stem will be decayed and become the waste product. Some indigenous bacteria species that live in the decayed ma...Mangrove （Sonneratia spp.） could be found at Waai seashore, Ambon island. The remainder of the mangrove stem will be decayed and become the waste product. Some indigenous bacteria species that live in the decayed mangrove stem waste product have cellulolytic and amylolytic characters. This research was done to： （1） identify and determine the cellulolytic bacteria species; （2） identify and determine the amylolytic bacteria species; （3） determine the cellulolytic bacteria species that have the highest cellulase activity; （4） determine the amylolytic bacteria species that have the highest amylase activity. This research was conducted at the Microbiology Laboratory, Department of Biology, State University of Malang, Microbiology Laboratory, Faculty of Medicine, Brawijaya University and Chemistry Laboratory, Muhammadiyah Malang University. In the experiment, 25 g sample were grinded and diluted in 225 mL nutrient broth to get 101 suspension. Then the suspension was diluted gradually until 101~. The suspension was inoculated on nutrient agar medium with 0.1 mL each, and incubated in 37 ~C during 24 h. Each bacteria colony was isolated and identified to know whether it was cellulolytic or amylolytic bacteria. Afterward, the cellulase activity as well as the amylase activity was analyzed. The research results show that： （1） there are four cellulolytic bacteria species, i.e., Micrococcus luteus, Bacillus pumilus, Planococcus citreus and Bacillus cereus; （2） there are four amylolytic bacteria species, i.e., Bacillus firmus, Nitrobacter sp., Bacillus mycoides and Pseudomonas aeruginosa; （3） Bacillus cereus has the highest cellulase activity; （4） Nitrobacter sp. has the highest amylase activity.展开更多
基金supported by the National Key Technologies R&D Program of China(2011BAD13B03)
文摘The RNA helicase Vasa is an important regulator of primordial germ cell development. Its function in mature fish, espe- cially the hormone-related differences in maturing male fish has seldom been documented. In this study, a full length cDNA sequence of the vasa gene was cloned from Japanese sea bass, Lateolabraxjaponicas, and it was namedjsb-vasa. Homology analysis showed thatjsb-vasa was closely related to its teleost homologs. The spatial distribution ofjsb-vasa indicated that it was only highly ex- pressed in testis, showing its germ cell-specific expression pattern. During the testicular development cycle, jsb-vasa was highly expressed during early period of spermatogenesis, and reduced when spermatogenesis advanced. In addition, the jsb-vasa gene ex- pression was significantly inhibited at 6 h, 12 h and 24 h after injecting hCG （human ehorionic gonadotropin） and GnRHa （Gonad- otropin-releasing hormone analogue）, indicating thatjsb-vasa gene may play an important role in spermatogenesis of Japanese sea bass, and be under the regulation of external sex hormones.
文摘Mangrove （Sonneratia spp.） could be found at Waai seashore, Ambon island. The remainder of the mangrove stem will be decayed and become the waste product. Some indigenous bacteria species that live in the decayed mangrove stem waste product have cellulolytic and amylolytic characters. This research was done to： （1） identify and determine the cellulolytic bacteria species; （2） identify and determine the amylolytic bacteria species; （3） determine the cellulolytic bacteria species that have the highest cellulase activity; （4） determine the amylolytic bacteria species that have the highest amylase activity. This research was conducted at the Microbiology Laboratory, Department of Biology, State University of Malang, Microbiology Laboratory, Faculty of Medicine, Brawijaya University and Chemistry Laboratory, Muhammadiyah Malang University. In the experiment, 25 g sample were grinded and diluted in 225 mL nutrient broth to get 101 suspension. Then the suspension was diluted gradually until 101~. The suspension was inoculated on nutrient agar medium with 0.1 mL each, and incubated in 37 ~C during 24 h. Each bacteria colony was isolated and identified to know whether it was cellulolytic or amylolytic bacteria. Afterward, the cellulase activity as well as the amylase activity was analyzed. The research results show that： （1） there are four cellulolytic bacteria species, i.e., Micrococcus luteus, Bacillus pumilus, Planococcus citreus and Bacillus cereus; （2） there are four amylolytic bacteria species, i.e., Bacillus firmus, Nitrobacter sp., Bacillus mycoides and Pseudomonas aeruginosa; （3） Bacillus cereus has the highest cellulase activity; （4） Nitrobacter sp. has the highest amylase activity.
