目的探讨创伤后应激障碍（Post-traumatic stress disorder，PTSD）对酒精条件性位置偏爱（conditioned place preference，CPP）获得的易化作用及其多巴胺机制。方法Sprague．Dawley（SD）雄性大鼠40只分为PTSD＋酒精（PE）组、正常对...目的探讨创伤后应激障碍（Post-traumatic stress disorder，PTSD）对酒精条件性位置偏爱（conditioned place preference，CPP）获得的易化作用及其多巴胺机制。方法Sprague．Dawley（SD）雄性大鼠40只分为PTSD＋酒精（PE）组、正常对照＋酒精（CE）组、PTSD＋生理盐水（PS）组和正常对照＋生理盐水（CS）组，每组大鼠进行CPP训练，酒精组给予腹腔注射20％酒精2g／kg，生理盐水组给予相同体积的生理盐水。PTSD动物模型建立采用单一持续性刺激（SPS）方式，通过僵立反应和高架十字迷宫分别测试SPS24h、7d的恐惧记忆及焦虑行为，在SPS7d检测训练后CPP值，并用免疫组织化学方法测定大鼠杏仁核区域多巴胺D1受体的表达。结果与正常对照组比较，仅在SPS7d时PTSD组大鼠僵立时间显著延长[（89．13±8．60）S，（22．25±5．85）S，q=8．77，P〈0．01]；以及进入开放臂次数、时间明显降低[（4．25±1．26）次，（14.38±2.18）次，（12．38＋-3．30）S，（40．38±7．29）S，q分别为4．74和4．08，均P〈0．01]；CPP测试中PE组训练后CPP值明显高于训练前，差异有统计学意义（q=31．81，P〈0．01），其余CE、PS及CS组训练前后CPP值均差异无统计学意义（q分别为1．53，0．01，0．27，P〉0．05）；PE组训练后的CPP值明显高于CS组、CE组和PS组（q分别为-38．32，-22．21，-33．38，P〈0．05）。SPS7d4组大鼠杏仁核区域多巴胺D1受体阳性细胞数免疫组化评分差异无统计学意义（F=0．07，P〉0．05）。结论PTSD样大鼠易于形成酒精条件性位置偏爱，可能与杏仁核多巴胺D1受体数量变化无关。展开更多
AIM： To investigate the role of intestinal mucosal blood flow （IMBF） and motility in the damage of intestinal mucosal barrier in rats with traumatic brain injury. METHODS： Sixty-four healthy male Wistar rats were ...AIM： To investigate the role of intestinal mucosal blood flow （IMBF） and motility in the damage of intestinal mucosal barrier in rats with traumatic brain injury. METHODS： Sixty-four healthy male Wistar rats were divided randomly into two groups： traumatic brain injury （TBI） group （n = 32）, rats with traumatic brain injury; and control group （n = 32）, rats with sham-operation. Each group was divided into four subgroups （n = 8） as 6, 12, 24 and 48 h after operation. Intestinal motility was measured by the propulsion ratio of a semi-solid colored marker （carbon-ink）. IMBF was measured with the laser-Doppler technique. Endotoxin and D-xylose levels in plasma were measured to evaluate the change of intestinal mucosal barrier function following TBI. RESULTS： The level of endotoxin was significantly higher in TBI group than in the control group at each time point （0.382 ± 0.014 EU/mL vs 0.102 ± 0.007 EU/mL, 0.466 ± 0.018 EU/mL vs 0.114 ± 0.021 EU/mL, 0.478± 0.029 EU/mL vs 0.112 ±- 0.018 EU/mL and 0.412± 0.036 EU/mL vs 0.108 ±0.011 EU/mL, P 〈 0.05）. D-xylose concentrations in plasma in TBI group were significantly higher than in the control group （6.68 ± 2.37 mmol/L vs 3.66 ±1.07 retool/L, 8.51 ± 2.69 mmol/L vs 3.15 ＋ 0.95 mmol/L, 11.68 ±3.24 mmol/L vs 3.78 ± 1.12 mmol/L and 10.23 ± 2.83 mmol/L vs 3.34 ± 1.23 mmol/L, P 〈 0.05）. The IMBF in TBI group was significantly lower than that in the control group （38.5 ± 2.8 PU vs 45.6 ± 4.6 PU, 25.2 ± 3.1 PU vs 48.2 ± 5.3 PU, 21.5 ± 2.7 PU vs 44.9 ± 2.8 PU, 29. 4 ± 3.8 PU vs 46.7 ± 3.2 PU） （P 〈 0.05）. Significant decelerations of intestinal propulsion ratio in T8I groups were found compared with the control group （0.48% ± 0.06% vs 0.62%± 0.03%, 0.37% ±0.05% vs 0.64% ± 0.01%, 0.39% ± 0.07% vs 0.63% =1= 0.05% and 0.46% ± 0.03% vs 0.65% ± 0.02%） （P 〈 0.05）. CONCLUSION： The intestinal mucosal permeability is increased obviously in TBI rats. Decrease of intestinal motility and IMBF occur early in TBI, both are important pathogenic factors for stress-related damage of the intestinal mucosal barrier in TBI.展开更多
Analgesis and wound healing effect of chitosan and carboxymethyl chitosan on scalded rats were investigated. A II degree scald model was established in rats, which was subsequently treated with chitosan and carboxymet...Analgesis and wound healing effect of chitosan and carboxymethyl chitosan on scalded rats were investigated. A II degree scald model was established in rats, which was subsequently treated with chitosan and carboxymethyl chitosan solution, respectively. The concentration of bradykinin and 5-hydroxytryptophan was detected by assaying enzyme-linked immunosorbent. Healing condition was observed and pathological sections were made to determine the healing effect of chitosan and carboxymethyl chitosan. Results showed that the concentration of bradykinin and 5-hydroxytryptophan peaked at the third hour post-wound in all groups, while the concentration of hydroxyproline peaked at the seventh day post-wound in both chitosan and carboxymethyl chitosan group. The concentration of bradykinin and 5-hydroxytryptophan of carboxymethyl chitosan group was significantly lower than that of control(P < 0.05), while that of chitosan group was similar to that of control(P > 0.05). These findings indicated that carboxymethyl chitosan reduced the concentration of algogenic substances, resulting in analgesia. During the whole recovery process, the hydroxyproline concentration in chitosan and carboxymethyl chitosan group on day 3 and 7 was significantly higher than that of control(P < 0.01); however the significance of such a highness decreased on day 14(P < 0.05). These findings indicated that chitosan and carboxymethyl chitosan accelerated tissue repair. Meanwhile, chitosan performed better in healing than carboxymethyl chitosan in both decrustation and healing time. In conclusion, carboxymethyl chitosan showed significant analgesis and wound-healing promotion effect, but chitosan only showed wound-healing promotion effect.展开更多
The effects of adipose-derived mesenchymal stem cell (ADMSC) transplantation for the repair of traumatic brain injury remain poorly understood. The present study observed neurological functional changes in a rat model...The effects of adipose-derived mesenchymal stem cell (ADMSC) transplantation for the repair of traumatic brain injury remain poorly understood. The present study observed neurological functional changes in a rat model of traumatic brain injury following ADMSC transplantation via the tail vein. Cell transplants were observed in injured cerebral cortex, and expression of brain-derived nerve growth factor was significantly increased in the injured hippocampus following transplantation. Results demonstrated that transvenous ADMSC transplants migrated to the injured cerebral cortex and significantly improved cognitive function.展开更多
文摘目的探讨创伤后应激障碍（Post-traumatic stress disorder，PTSD）对酒精条件性位置偏爱（conditioned place preference，CPP）获得的易化作用及其多巴胺机制。方法Sprague．Dawley（SD）雄性大鼠40只分为PTSD＋酒精（PE）组、正常对照＋酒精（CE）组、PTSD＋生理盐水（PS）组和正常对照＋生理盐水（CS）组，每组大鼠进行CPP训练，酒精组给予腹腔注射20％酒精2g／kg，生理盐水组给予相同体积的生理盐水。PTSD动物模型建立采用单一持续性刺激（SPS）方式，通过僵立反应和高架十字迷宫分别测试SPS24h、7d的恐惧记忆及焦虑行为，在SPS7d检测训练后CPP值，并用免疫组织化学方法测定大鼠杏仁核区域多巴胺D1受体的表达。结果与正常对照组比较，仅在SPS7d时PTSD组大鼠僵立时间显著延长[（89．13±8．60）S，（22．25±5．85）S，q=8．77，P〈0．01]；以及进入开放臂次数、时间明显降低[（4．25±1．26）次，（14.38±2.18）次，（12．38＋-3．30）S，（40．38±7．29）S，q分别为4．74和4．08，均P〈0．01]；CPP测试中PE组训练后CPP值明显高于训练前，差异有统计学意义（q=31．81，P〈0．01），其余CE、PS及CS组训练前后CPP值均差异无统计学意义（q分别为1．53，0．01，0．27，P〉0．05）；PE组训练后的CPP值明显高于CS组、CE组和PS组（q分别为-38．32，-22．21，-33．38，P〈0．05）。SPS7d4组大鼠杏仁核区域多巴胺D1受体阳性细胞数免疫组化评分差异无统计学意义（F=0．07，P〉0．05）。结论PTSD样大鼠易于形成酒精条件性位置偏爱，可能与杏仁核多巴胺D1受体数量变化无关。
文摘AIM： To investigate the role of intestinal mucosal blood flow （IMBF） and motility in the damage of intestinal mucosal barrier in rats with traumatic brain injury. METHODS： Sixty-four healthy male Wistar rats were divided randomly into two groups： traumatic brain injury （TBI） group （n = 32）, rats with traumatic brain injury; and control group （n = 32）, rats with sham-operation. Each group was divided into four subgroups （n = 8） as 6, 12, 24 and 48 h after operation. Intestinal motility was measured by the propulsion ratio of a semi-solid colored marker （carbon-ink）. IMBF was measured with the laser-Doppler technique. Endotoxin and D-xylose levels in plasma were measured to evaluate the change of intestinal mucosal barrier function following TBI. RESULTS： The level of endotoxin was significantly higher in TBI group than in the control group at each time point （0.382 ± 0.014 EU/mL vs 0.102 ± 0.007 EU/mL, 0.466 ± 0.018 EU/mL vs 0.114 ± 0.021 EU/mL, 0.478± 0.029 EU/mL vs 0.112 ±- 0.018 EU/mL and 0.412± 0.036 EU/mL vs 0.108 ±0.011 EU/mL, P 〈 0.05）. D-xylose concentrations in plasma in TBI group were significantly higher than in the control group （6.68 ± 2.37 mmol/L vs 3.66 ±1.07 retool/L, 8.51 ± 2.69 mmol/L vs 3.15 ＋ 0.95 mmol/L, 11.68 ±3.24 mmol/L vs 3.78 ± 1.12 mmol/L and 10.23 ± 2.83 mmol/L vs 3.34 ± 1.23 mmol/L, P 〈 0.05）. The IMBF in TBI group was significantly lower than that in the control group （38.5 ± 2.8 PU vs 45.6 ± 4.6 PU, 25.2 ± 3.1 PU vs 48.2 ± 5.3 PU, 21.5 ± 2.7 PU vs 44.9 ± 2.8 PU, 29. 4 ± 3.8 PU vs 46.7 ± 3.2 PU） （P 〈 0.05）. Significant decelerations of intestinal propulsion ratio in T8I groups were found compared with the control group （0.48% ± 0.06% vs 0.62%± 0.03%, 0.37% ±0.05% vs 0.64% ± 0.01%, 0.39% ± 0.07% vs 0.63% =1= 0.05% and 0.46% ± 0.03% vs 0.65% ± 0.02%） （P 〈 0.05）. CONCLUSION： The intestinal mucosal permeability is increased obviously in TBI rats. Decrease of intestinal motility and IMBF occur early in TBI, both are important pathogenic factors for stress-related damage of the intestinal mucosal barrier in TBI.
基金supported by National High-Tech R&D Program of China (863 Program, 2014AA093605)
文摘Analgesis and wound healing effect of chitosan and carboxymethyl chitosan on scalded rats were investigated. A II degree scald model was established in rats, which was subsequently treated with chitosan and carboxymethyl chitosan solution, respectively. The concentration of bradykinin and 5-hydroxytryptophan was detected by assaying enzyme-linked immunosorbent. Healing condition was observed and pathological sections were made to determine the healing effect of chitosan and carboxymethyl chitosan. Results showed that the concentration of bradykinin and 5-hydroxytryptophan peaked at the third hour post-wound in all groups, while the concentration of hydroxyproline peaked at the seventh day post-wound in both chitosan and carboxymethyl chitosan group. The concentration of bradykinin and 5-hydroxytryptophan of carboxymethyl chitosan group was significantly lower than that of control(P < 0.05), while that of chitosan group was similar to that of control(P > 0.05). These findings indicated that carboxymethyl chitosan reduced the concentration of algogenic substances, resulting in analgesia. During the whole recovery process, the hydroxyproline concentration in chitosan and carboxymethyl chitosan group on day 3 and 7 was significantly higher than that of control(P < 0.01); however the significance of such a highness decreased on day 14(P < 0.05). These findings indicated that chitosan and carboxymethyl chitosan accelerated tissue repair. Meanwhile, chitosan performed better in healing than carboxymethyl chitosan in both decrustation and healing time. In conclusion, carboxymethyl chitosan showed significant analgesis and wound-healing promotion effect, but chitosan only showed wound-healing promotion effect.
基金the National Basic Research Program of China(973Program),No.2007CB512705the General Program for Youths of the National Natural Science Foundation of China,No.30801464
文摘The effects of adipose-derived mesenchymal stem cell (ADMSC) transplantation for the repair of traumatic brain injury remain poorly understood. The present study observed neurological functional changes in a rat model of traumatic brain injury following ADMSC transplantation via the tail vein. Cell transplants were observed in injured cerebral cortex, and expression of brain-derived nerve growth factor was significantly increased in the injured hippocampus following transplantation. Results demonstrated that transvenous ADMSC transplants migrated to the injured cerebral cortex and significantly improved cognitive function.
