Objective： To investigate the expression pattern of resistin （RSTN） in skeletal muscle tissue and its influence on glycometabolism in rats with traumatic brain injury （TBI）. Methods： Seventy-eight SD rats were ...Objective： To investigate the expression pattern of resistin （RSTN） in skeletal muscle tissue and its influence on glycometabolism in rats with traumatic brain injury （TBI）. Methods： Seventy-eight SD rats were randomly divided into traumatic group （n=36）, RSTN group （n=36） and sham operation group （n=6）. Fluid percussion TBI model was developed in traumatic and RSTN groups and the latter received additional 1 mg RSTN antibody treatment for each rat. At respectively 12 h, 24 h, 72 h, 1 w, 2 w, and 4 w after operation, venous blood was collected and the right hind leg skeletal muscle tissue was sampled. We used real-time PCR to determine mRNA expression of RSTN in skeletal muscles, western blot to determine RSTN protein expression and ELISA to assess serum insulin as well as fasting blood glucose （FBG） levels. Calculation of the quantitative insulin sensitivity check index （Q value） was also conducted. The above mentioned indicators and their correction were statistically analyzed. Results： Compared with sham operation group, the RSTN expression in the skeletal muscle as well as serum insulin and FBG levels revealed significant elevation （P〈0.05）, and reduced Q value （P〈0.05） in traumatic group. Single factor linear correlation analysis showed a significant negative correlation between RSTN expression and Q values （P〈0.001） in traumatic group. Conclusion： The expression of RSTN has been greatly increased in the muscular tissue of TBI rats and it was closely related to the index of glycometabolism. RSTN may play an important role in the process of insulin resistance after TBI.展开更多
According to medical literature, fracture of the first rib is quite rare and the bilateral condition is especially rare. This type of fracture is usually associated with severe intrathoracic trauma and other bony or n...According to medical literature, fracture of the first rib is quite rare and the bilateral condition is especially rare. This type of fracture is usually associated with severe intrathoracic trauma and other bony or neurovascular injuries, thus can be considered as a harbinger of major trauma. However here we present three cases of low velocity first rib fractures without any major trauma or multisystem injuries. All the three patients were treated conservatively and did well on simple analgesics and rest and had no early or late complications. It can be seen that not all the first rib fractures are associated with major trauma or multisystem injuries. There is a variant of first rib fracture with low velocity injuries which is not associated with any major complications in contrast to majority of first rib fractures associated with high velocity injuries. Causative factor of such injuries may be violent muscular contraction of scalenus anterior or serratus anterior, but not direct trauma.展开更多
文摘Objective： To investigate the expression pattern of resistin （RSTN） in skeletal muscle tissue and its influence on glycometabolism in rats with traumatic brain injury （TBI）. Methods： Seventy-eight SD rats were randomly divided into traumatic group （n=36）, RSTN group （n=36） and sham operation group （n=6）. Fluid percussion TBI model was developed in traumatic and RSTN groups and the latter received additional 1 mg RSTN antibody treatment for each rat. At respectively 12 h, 24 h, 72 h, 1 w, 2 w, and 4 w after operation, venous blood was collected and the right hind leg skeletal muscle tissue was sampled. We used real-time PCR to determine mRNA expression of RSTN in skeletal muscles, western blot to determine RSTN protein expression and ELISA to assess serum insulin as well as fasting blood glucose （FBG） levels. Calculation of the quantitative insulin sensitivity check index （Q value） was also conducted. The above mentioned indicators and their correction were statistically analyzed. Results： Compared with sham operation group, the RSTN expression in the skeletal muscle as well as serum insulin and FBG levels revealed significant elevation （P〈0.05）, and reduced Q value （P〈0.05） in traumatic group. Single factor linear correlation analysis showed a significant negative correlation between RSTN expression and Q values （P〈0.001） in traumatic group. Conclusion： The expression of RSTN has been greatly increased in the muscular tissue of TBI rats and it was closely related to the index of glycometabolism. RSTN may play an important role in the process of insulin resistance after TBI.
文摘According to medical literature, fracture of the first rib is quite rare and the bilateral condition is especially rare. This type of fracture is usually associated with severe intrathoracic trauma and other bony or neurovascular injuries, thus can be considered as a harbinger of major trauma. However here we present three cases of low velocity first rib fractures without any major trauma or multisystem injuries. All the three patients were treated conservatively and did well on simple analgesics and rest and had no early or late complications. It can be seen that not all the first rib fractures are associated with major trauma or multisystem injuries. There is a variant of first rib fracture with low velocity injuries which is not associated with any major complications in contrast to majority of first rib fractures associated with high velocity injuries. Causative factor of such injuries may be violent muscular contraction of scalenus anterior or serratus anterior, but not direct trauma.
