The premise of this abstract focuses on lessons learned as a clinician through the process of being invited into the homes, lives and stories of trauma and incredible resilience for native survivors of Indian resident...The premise of this abstract focuses on lessons learned as a clinician through the process of being invited into the homes, lives and stories of trauma and incredible resilience for native survivors of Indian residential schools. Through the process of unravelling stories of past trauma with a view to recovery/freedom from symptoms, meaningful therapy requires the weaving together of traditional beliefs and practices with trauma focused cognitive behavioral therapy approaches. Culturally, the relevant treatment respects traditional beliefs and focuses on their innate resiliency historically within their culture. As a clinician invited intimately through the therapeutic relationship into the lives of First Nations abuse survivors, the healing power of cultural identity was discovered along them. Similarly, the author's role as therapist was one of teachers, who guided them through the process of telling their stories to the peace and acceptance utilizing therapeutic techniques from an eclectic spectrum of therapeutic approaches. All the while, it should interweave their stories and native beliefs into the therapy journey. It was a process of co-creating healing through a mutual respect of the shared roles as guide, teacher, story teller and healer. Culturally, the relevant psychotherapy requires the weaving of two world views to create a basket of healing.展开更多
Objective: To study the role of dendritic cells (DCs) in initiating delayed-type hypersensitivity (DTH) to fluores- cein isothiocyanate (FITC) after trauma-hemorrhage in mice. Methods: Inbred BALB/c mice (6-...Objective: To study the role of dendritic cells (DCs) in initiating delayed-type hypersensitivity (DTH) to fluores- cein isothiocyanate (FITC) after trauma-hemorrhage in mice. Methods: Inbred BALB/c mice (6-8 weeks old, male) were epicutaneously sensitized with FITC 12 hours, 1 day, 2 days, 4 days and 7 days after closed bilateral femur fractures combined with hemorrhage. And 5 days after sensitization, DTH was evaluated by ear swelling after a challenge of FITC. Draining lymph node cells were examined for the percentages of FITC-positive cells, cluster of differentiation (CD)11c positive cells and major histocom- patibility complex II (MHC II)-positive cells by means of flow cytometry. In vitro proliferative responses of syngeneic lymphocytes and in vivo passive transfer of DTH to naive recipients induced by isolated DCs from the draining lymph nodes were determined. Results: The time of DTH to FITC decreased more significantly in the mice with trauma-hemorrhage (12 hours to 4 days) than in the mice with sham injury. After sensitization, the relative percentages of FITC^+ cells, FITC^+/ CD 11c^+ cells and FITC^+/CD 11c^+/MHC II^+ cells from the draining lymph nodes were all significantly reduced following injury. And the capacity of DCs from the draining lymph nodes in stimulating proliferative responses of lymphocytes and transferring DTH to naive recipients were also inhibited after injury. Conclusions: Trauma-hemorrhage induces repressive DTH in mice, which may be attributed, at least partially, to the reduced trafficking of DCs into the draining lymph nodes and insufficient maturation during DC migration.展开更多
文摘The premise of this abstract focuses on lessons learned as a clinician through the process of being invited into the homes, lives and stories of trauma and incredible resilience for native survivors of Indian residential schools. Through the process of unravelling stories of past trauma with a view to recovery/freedom from symptoms, meaningful therapy requires the weaving together of traditional beliefs and practices with trauma focused cognitive behavioral therapy approaches. Culturally, the relevant treatment respects traditional beliefs and focuses on their innate resiliency historically within their culture. As a clinician invited intimately through the therapeutic relationship into the lives of First Nations abuse survivors, the healing power of cultural identity was discovered along them. Similarly, the author's role as therapist was one of teachers, who guided them through the process of telling their stories to the peace and acceptance utilizing therapeutic techniques from an eclectic spectrum of therapeutic approaches. All the while, it should interweave their stories and native beliefs into the therapy journey. It was a process of co-creating healing through a mutual respect of the shared roles as guide, teacher, story teller and healer. Culturally, the relevant psychotherapy requires the weaving of two world views to create a basket of healing.
基金This study was supported by the National Basic Research Program of China (No. 2005CB522602), Natural Science Foundation of China (No.30772253) and Project of State Key Laboratory of Trauma, Burns, and Combined Injury (No. SKLZZ200802).
文摘Objective: To study the role of dendritic cells (DCs) in initiating delayed-type hypersensitivity (DTH) to fluores- cein isothiocyanate (FITC) after trauma-hemorrhage in mice. Methods: Inbred BALB/c mice (6-8 weeks old, male) were epicutaneously sensitized with FITC 12 hours, 1 day, 2 days, 4 days and 7 days after closed bilateral femur fractures combined with hemorrhage. And 5 days after sensitization, DTH was evaluated by ear swelling after a challenge of FITC. Draining lymph node cells were examined for the percentages of FITC-positive cells, cluster of differentiation (CD)11c positive cells and major histocom- patibility complex II (MHC II)-positive cells by means of flow cytometry. In vitro proliferative responses of syngeneic lymphocytes and in vivo passive transfer of DTH to naive recipients induced by isolated DCs from the draining lymph nodes were determined. Results: The time of DTH to FITC decreased more significantly in the mice with trauma-hemorrhage (12 hours to 4 days) than in the mice with sham injury. After sensitization, the relative percentages of FITC^+ cells, FITC^+/ CD 11c^+ cells and FITC^+/CD 11c^+/MHC II^+ cells from the draining lymph nodes were all significantly reduced following injury. And the capacity of DCs from the draining lymph nodes in stimulating proliferative responses of lymphocytes and transferring DTH to naive recipients were also inhibited after injury. Conclusions: Trauma-hemorrhage induces repressive DTH in mice, which may be attributed, at least partially, to the reduced trafficking of DCs into the draining lymph nodes and insufficient maturation during DC migration.