AIM： To investigate the effects of KN-93, a CaMKⅡ selective inhibitor on cell proliferation and the expression of p53 or p21 protein in human hepatic stellate cells. METHODS： Human hepatic stellate cells （LX-2） w...AIM： To investigate the effects of KN-93, a CaMKⅡ selective inhibitor on cell proliferation and the expression of p53 or p21 protein in human hepatic stellate cells. METHODS： Human hepatic stellate cells （LX-2） were incubated with various concentrations （0-50 μmol/L） of KN-93 or its inactive derivative, KN-92. Cell proliferation was measured by CCK-8 assay, and the expression of two cell cycle regulators, p53 and p21, was determined by SDS-PAGE and Western blotting. RESULTS： KN-93 （5-50 μmol/L） decreased the proliferation of human hepatic stellate cells in a dosedependent manner from 81.76% （81.76% ± 2.58% vs 96.63% ± 2.69%, P 〈 0.05） to 27.15% （27.15% ± 2.86% vs 96.59% ± 2.44%, P 〈 0.01） after 24 h treatment. Incubation of 10 μmol/L KN-93 induced the cell growth reduction in a time-dependent manner from 78.27% at 8 h to 11.48% at 48 h. However, KN-92, an inactive derivative of KN-93, did not inhibit cell proliferation effectively. Moreover, analysis of cell cycle regulator expression revealed that KN-93 rather than KN-92 reduced the expression of p53 and p21. CONCLUSION： KN-93 has potent inhibitory effect on proliferation of LX-2 cells by modulating the expression of two special cell cycle regulators, p53 and p21.展开更多
Objective: To explore the application of controlled hypothermia radio-frequency ablation technique in otolaryngology. Methods: Germany Bipolar controlled hypothermia radio-frequency with varying tips was used for obst...Objective: To explore the application of controlled hypothermia radio-frequency ablation technique in otolaryngology. Methods: Germany Bipolar controlled hypothermia radio-frequency with varying tips was used for obstructive sleep apnea-hyponea syndrome, chronic hypertrophic rhinitis, allergic rhinitis, aural pseudocyst, phymatoid proliferation of tongue root, capillary hemangioma of nose and pharynx. Follow up was made up to six months. Results:All patients had curative effects. Conclusion: Hypothermia radio-frequency ablation technique was used effectively for many diseases in otolaryngology with features of accurate ablation, low complication, reliable effects as well as easyusing. It was a prospective method of micro invasive surgery.展开更多
The purpose of this study was to investigate the effect of Laggera alata flavonen （LAF） on the inhibit- ing effect of human ovarian cancer HO-8910 cells proliferation and its possible mechanism in vitro. Methods： H...The purpose of this study was to investigate the effect of Laggera alata flavonen （LAF） on the inhibit- ing effect of human ovarian cancer HO-8910 cells proliferation and its possible mechanism in vitro. Methods： Human ovarian cancer HO-8910 cells were cultured in vitro. Inhibitory effect of LAF on the viability of HO-8910 cells was evaluated by the MTT assay. Apoptotic effect of different concentrations of LAF on HO-8910 cells was assessed by AO/EB staining and FCM with propidium iodide （PI） staining. Expression of proteins related to apoptosis was analyzed by Western blot. Results： LAF significantly inhibited the viability of HO-8910 cells proliferation in a dose-dependent and time-dependent manner, there were statistical significance compared with NS group （P 〈 0.05）, and the ICso was 4.28 pg/mL for 48 h. The cells treated with LAF showed typical morphological change and apoptotic rate increased by FCM in a dose-dependent, and there was notable dif- ference compared with NS group （P 〈 0.05）. Western blot showed that expression of Fas, caspase-8, tBid and Cyto-c proteins were up-regulated after treatment with LAF for 48 h in a concentration dependent. Conclusion： LAF could inhibit HO-8910 cells proliferation and induce apoptosis, which may be through the pathway of death receptor in vitro.展开更多
In recent years, with the further development of our country＇s financial management system, the existing budget accounting system can not adapt to the current management system, many problems are exposed in the actua...In recent years, with the further development of our country＇s financial management system, the existing budget accounting system can not adapt to the current management system, many problems are exposed in the actual operation. Therefore, we must proceed from the actual situation, using the scientific method, reform of the budget accounting system, promote the budget accounting system more scientific and perfect. This article will carry on problems existing in the current budget accounting system analysis, put forward the reform ideas and suggestions accordingly, with a view to the reform and development of the budget accounting to meet the objective demand of the development of the times.展开更多
this paper based on the food security, the food the macroeconomic regulation and control of the operation mechanism of the general theory, focus on the China＇s grain macroeconomic regulation and control mechanism, th...this paper based on the food security, the food the macroeconomic regulation and control of the operation mechanism of the general theory, focus on the China＇s grain macroeconomic regulation and control mechanism, the concept of grain macroeconomic regulation and control mechanism, the grain circulation macroeconomic regulation and control tool application, grain circulation macroeconomic regulation and control system, the problem of grain circulation system, food price formation mechanism. Through the research set up grain control of the operation mechanism of general theory.展开更多
文摘AIM： To investigate the effects of KN-93, a CaMKⅡ selective inhibitor on cell proliferation and the expression of p53 or p21 protein in human hepatic stellate cells. METHODS： Human hepatic stellate cells （LX-2） were incubated with various concentrations （0-50 μmol/L） of KN-93 or its inactive derivative, KN-92. Cell proliferation was measured by CCK-8 assay, and the expression of two cell cycle regulators, p53 and p21, was determined by SDS-PAGE and Western blotting. RESULTS： KN-93 （5-50 μmol/L） decreased the proliferation of human hepatic stellate cells in a dosedependent manner from 81.76% （81.76% ± 2.58% vs 96.63% ± 2.69%, P 〈 0.05） to 27.15% （27.15% ± 2.86% vs 96.59% ± 2.44%, P 〈 0.01） after 24 h treatment. Incubation of 10 μmol/L KN-93 induced the cell growth reduction in a time-dependent manner from 78.27% at 8 h to 11.48% at 48 h. However, KN-92, an inactive derivative of KN-93, did not inhibit cell proliferation effectively. Moreover, analysis of cell cycle regulator expression revealed that KN-93 rather than KN-92 reduced the expression of p53 and p21. CONCLUSION： KN-93 has potent inhibitory effect on proliferation of LX-2 cells by modulating the expression of two special cell cycle regulators, p53 and p21.
文摘Objective: To explore the application of controlled hypothermia radio-frequency ablation technique in otolaryngology. Methods: Germany Bipolar controlled hypothermia radio-frequency with varying tips was used for obstructive sleep apnea-hyponea syndrome, chronic hypertrophic rhinitis, allergic rhinitis, aural pseudocyst, phymatoid proliferation of tongue root, capillary hemangioma of nose and pharynx. Follow up was made up to six months. Results:All patients had curative effects. Conclusion: Hypothermia radio-frequency ablation technique was used effectively for many diseases in otolaryngology with features of accurate ablation, low complication, reliable effects as well as easyusing. It was a prospective method of micro invasive surgery.
文摘The purpose of this study was to investigate the effect of Laggera alata flavonen （LAF） on the inhibit- ing effect of human ovarian cancer HO-8910 cells proliferation and its possible mechanism in vitro. Methods： Human ovarian cancer HO-8910 cells were cultured in vitro. Inhibitory effect of LAF on the viability of HO-8910 cells was evaluated by the MTT assay. Apoptotic effect of different concentrations of LAF on HO-8910 cells was assessed by AO/EB staining and FCM with propidium iodide （PI） staining. Expression of proteins related to apoptosis was analyzed by Western blot. Results： LAF significantly inhibited the viability of HO-8910 cells proliferation in a dose-dependent and time-dependent manner, there were statistical significance compared with NS group （P 〈 0.05）, and the ICso was 4.28 pg/mL for 48 h. The cells treated with LAF showed typical morphological change and apoptotic rate increased by FCM in a dose-dependent, and there was notable dif- ference compared with NS group （P 〈 0.05）. Western blot showed that expression of Fas, caspase-8, tBid and Cyto-c proteins were up-regulated after treatment with LAF for 48 h in a concentration dependent. Conclusion： LAF could inhibit HO-8910 cells proliferation and induce apoptosis, which may be through the pathway of death receptor in vitro.
文摘In recent years, with the further development of our country＇s financial management system, the existing budget accounting system can not adapt to the current management system, many problems are exposed in the actual operation. Therefore, we must proceed from the actual situation, using the scientific method, reform of the budget accounting system, promote the budget accounting system more scientific and perfect. This article will carry on problems existing in the current budget accounting system analysis, put forward the reform ideas and suggestions accordingly, with a view to the reform and development of the budget accounting to meet the objective demand of the development of the times.
文摘this paper based on the food security, the food the macroeconomic regulation and control of the operation mechanism of the general theory, focus on the China＇s grain macroeconomic regulation and control mechanism, the concept of grain macroeconomic regulation and control mechanism, the grain circulation macroeconomic regulation and control tool application, grain circulation macroeconomic regulation and control system, the problem of grain circulation system, food price formation mechanism. Through the research set up grain control of the operation mechanism of general theory.
