抗极低密度脂蛋白受体多克隆抗体的制备及鉴定

为制备抗极低密度脂蛋白受体的多克隆抗体 ,以极低密度脂蛋白受体配体结合域上特定片段的合成肽免疫新西兰家兔 ,收集免疫前后动物的血清 ,通过酶联免疫吸附法及免疫印迹法对血清中抗体进行分析和鉴定。结果发现 ,已获得高效价的抗极低密度脂蛋白受体血清 ,可利用免疫印迹法检测人、鼠组织和细胞中的天然极低密度脂蛋白受体及其亚型的蛋白。此结果提示 ,抗极低密度脂蛋白受体多克隆抗体的制备为从蛋白水平研究极低密度脂蛋白受体提供了有效的工具。

Preparation of ultrafine silver powders with controllable size and morphology

The ultrafine silver powders were prepared by liquid reduction method using Arabic gum as dispersant.The effects of different dispersants,pH values,and temperature on the morphology and particle size of silver powders were investigated.It is found that Arabic gum can better adsorb on silver particles via chemical adsorption,and it shows the best dispersive effect among all the selected dispersants.The particle size of silver powders can be finely tuned from 0.34 to 4.09μm by adjusting pH values,while the morphology of silver powders can be tuned by changing the temperature.The silver powders with high tap density higher than 4.0 g/cm3 were successfully prepared in a wide temperature range of 21.8-70°C.Especially,the tap density is higher than 5.0 g/cm3 when the temperature is optimized to be 50°C.The facile process and high silver concentration of this method make it a promising way to prepare high quality silver powders for electronic paste.

一种改进的蔗糖线性密度梯度溶液制备法

[目的]制备具有良好线性的蔗糖密度梯度溶液。[方法]利用溶液梯度混合仪和蠕动泵组装成新型的蔗糖密度梯度溶液制备器,用该制备器制备50%~0%和50%~10%等不同蔗糖密度梯度溶液,然后利用电脑核酸蛋白检测仪检测所制备的蔗糖密度梯度溶液的光密度,并对蔗糖密度溶液的线性进行评估;比较自制的蔗糖密度梯度溶液制备器与经典的Biocomp全自动密度梯度制备仪制备的蔗糖密度梯度溶液的优劣。[结果]自制的蔗糖密度梯度溶液制备器制备的50%~0%和50%~10%蔗糖密度梯度溶液的光密度与蔗糖密度的决定系数(R^(2))都在0.98以上;Biocomp全自动密度梯度制备仪制备的50%~0%蔗糖密度梯度溶液的光密度与蔗糖密度的决定系数(R^(2))为0.95~0.97。[结论]自制的蔗糖密度梯度溶液制备器制备的蔗糖密度梯度溶液优于Biocomp全自动密度梯度制备仪制备的蔗糖密度梯度溶液。

PREPARATION OF PARTICLE SIZE NARROWLY DISTRIBUTED LOW-DENSITY STYRENE DIVINYLBENZENE COPOLYMER MICROBEADS

In view of the importance of dispersion agent,the amount of the crosslinking monomer and the diluent in suspension polymerization for the production of particle size narrowly distributed low-density stryene divinylbenzene copolymer microbeads(LDPS),their actions are preliminarily investigated in this paper.Experimental results indicate that when both the gelatine and polyvinyl alcohol(PVA)are used dispersion agents,the better effect is achieved,DVB is helpful to the formation of the lower density fine particles,the proportionof the DVB/St should be between 1:1-1.5:1.Compared with toluene,gasoline is the more effective diluent for the above target.

Fast synthesis of uniform mesoporous titania submicrospheres with high tap densities for high-volumetric performance Li-ion batteries

High-tap density electrode materials are greatly desired for Li-ion batteries with high volumetric capacities to fulfill the growing demands of electric vehicles and portable smart devices. TiOz, which is one of the most attractive an- ode materials, is limited in their application for Li-ion batteries because of its low tap density （usually 〈1 gcm-3） and volumetric capacity. Herein, we report uniform mesoporous TiO2 submicrospheres with a tap density as high as 1.62 gcm-3 as a promising anode material. Even with a high mass load- ing of 24 mg cm-2, the TiO2 submicrospheres have impressive volumetric capacities that are more than double those of their counterparts. Moreover, they can be synthesized with -100% yield and within a reaction time of -6 h by optimizing the experimental conditions and formation mechanism, exhibiting potential for large-scale production for industrial applications. Other mesoporous anode materials, i.e., hightap density mesoporous Li4Ti5O12 submicrospheres, are fabricated using the generalized method. We believe that our work provides a significant reference for the industrial production of mesoporous materials for Li-ion batteries with a high volumetric performance.

Fabrication of 150-nm Al_(0.48)In_(0.52)As/Ga_(0.47)In_(0.53)As mHEMTs on GaAs substrates

High performance 150-nm gate-length metamorphic Al0.48In0.52As/Ga0.47In0.53 As high electron mobility transistors(mHEMTs) with very good device performance have been successfully fabricated.A T-shaped gate is fabricated by using a combined technique of optical and e-beam photolithography,which is beneficial to decreasing parasitic capacitance and parasitic resistance of the gate.The ohmic contact resistance R c is as low as 0.03 mm when using a novel ohmic contact metal system(Ni/Ge/Ti/Au).The devices exhibit excellent DC and RF performance.A peak extrinsic transconductance of 775 mS/mm and a maximum drain current density of 720 mA/mm are achieved.The unity current gain cut-off frequency(fT) and the maximum oscillation frequency(f max) are 188.4 and 250 GHz,respectively.

Reduced CoNi2S4 nanosheets decorated by sulfur vacancies with enhanced electrochemical performance for asymmetric supercapacitors

Nowadays,it is a matter of great concern to design electrode materials with excellent electrochemical performance for supercapacitors by a safe,efficient and simple method.And these characteristics are usually related to the vacancies and impurities in the electrode.To investigate the effect of the vacancies on the electrochemical properties of the supercapacitor cathode material,the uniform reduced CoNi2S4(r-CoNi2S4)nanosheets with sulfur vacancies have been successfully prepared by a one-step hydrothermal method.And the formation of sulfur vacancies are characterized by Raman,X-ray photoelectron spectroscopy and other means.As the electrode for supercapacitor,the r-CoNi2S4 nanosheet electrode delivers a high capacity of 1918.9 Fg-1 at a current density of 1 A g-1,superior rate capability(87.9%retention at a current density of 20 A g-1)and extraordinary cycling stability.Compared with the original CoNi2S4 nanosheet electrode(1226 F g-1at current density of 1 A g-1),the r-CoNi2S4 nanosheet electrode shows a great improvement.The asymmetric supercapacitor based on the r-CoNi2S4 positive electrode and activated carbon negative electrode exhibits a high energy density of 30.3 Wh kg-1 at a power density of 802.1 W kg-1,as well as excellent long-term cycling stability.The feasibility and great potential of the device in practical applications have been successfully proved by lightening the light emitting diodes of three different colors.

Copper/cuprous sulfide electrode: preparation and performance

Cuprous sulfide （Cu2S） is a direct band-gap p-type semiconductor with excellent ionic/electronic hybrid conductivity. Alt- hough Cu/Cu2S/sulfide or polysulfide system is adopted as counter electrode of quantum-dots-sensitized solar cells （QDSSC）, the electrode process is seldom reported. Here, the electrochemical growth of Cu2S film on a copper （Cu） surface, the redox behaviors of sulfide and polysulfide, and the all-in-solid charge-transfer properties of Cu2S film are investigated. It is clarified that the copper electrode simultaneously undergoes an activated process, a membrane growth process, and a redox phase transformation process. The solid charge-transfer capability of CuzS is quantified with a high exchange-current density of 2.27 A/cm2, which elucidates that the Cu/CuzS electrode is a qualified material for counter electrodes of QDSSC. These results aid understanding of the physicochemical mechanism of QDSSC with a polysulfide electrolyte and Cu/Cu2S counter electrode.

Cracked bark-inspired ternary metallic sulfide(NiCoMnS_(4)) nanostructure on carbon cloth for high-performance aqueous asymmetric supercapacitors

In this paper,we report a high-performance selfsupported supercapacitor electrode composed of a cracked bark-shaped Ni-Co-Mn ternary metallic sulfide(NiCoMnS4)nanostructure on carbon cloth prepared by a simple one-step hydrothermal process and subsequent electrochemical treatment.The electrode delivers a high specific discharge capacity of up to 2470.4 F g^(-1) at 1 A g^(-1) and high rate performances of1635.6 F g^(-1) at 10 A g^(-1) and 910.2 F g^(-1) even at 32 A g^(-1).Cycling tests indicate that NiCoMnS_(4) could maintain >91.1% of its initial capacity and nearly 100% Coulombic efficiency over10,000 cycles at 8 A g^(-1).An aqueous asymmetric supercapacitor assembled with NiCoMnS_(4) as the cathode,activated carbon as the anode,and 1 mol L^(-1) KOH as the electrolyte delivers an energy density of 68.2 W h kg^(-1)at 850.1 W kg^(-1) and capacity retention of 92.5% after 10,000 cycles at 4 A g^(-1).Given the excellent performance and simple material preparation of our proposed device,this study provides a valuable foundation for the development of self-supported metallic sulfide-based electrodes with high electrochemical properties for potential application in aqueous asymmetric supercapacitors.

Ferroelectric materials for vibrational energy harvesting

Energy harvesting is an appealing technology that makes use of the ambient energy which is otherwise wasted. Piezoelectric materials directly convert the elastic energy to the electric energy, and thus have a great advantage in scavenging vibrational energy for simplicity in device structure with relatively high power density. This paper provides an overview on the research of piezoelectric materials in energy harvesting in recent decades, from basics of piezoelectricity and working principle of energy harvesting with piezoelectric materials, to the progress of development of high-performance piezoelectrics including ceramics, single crystals and polymers, then to experimental attempts on the device fabrication and optimization, finally to perspective applications of piezoelectric energy harvesting(PEH). The criteria for selection of materials for PEH applications are introduced. Not only the figure of merit but also maximum allowable stress of materials are taken into account in the evaluation of their potential in achieving high energy density and output power density. The influence of the device configuration on the performance is also acknowledged and discussed. The magnitude and distribution of induced stress in the piezoelectric unit upon excitation by the vibration source play an important role in determining the output power density and can be tuned via proper design of device configuration without changing its resonant frequency. Approaches to address the issue of frequency match accompanying with the resonant mode are illustrated with literature examples. Usage of PEH devices can be extended to a variety of vibration sources in everyday life as well as in nature. Some appealing applications of PEH, such as in implantable and wearable devices, are reviewed.