This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase 2, 9 (MMP 2, MMP 9), tissue inhibitor 1 of matrix metalloproteinase (TIMP 1), c...This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase 2, 9 (MMP 2, MMP 9), tissue inhibitor 1 of matrix metalloproteinase (TIMP 1), cell adhesion molecule 44 variant 6 (CD44v6), HER2/neu and p53 was investigated in 154 patients with head and neck squamous cell carcinoma (SCC) by ABC and ImmunoMax immunohistochemical method. Their clinical relevance and correlation were analysed. The expression of MMP 2, MMP 9, TIMP 1, CD44v6, HER2/neu and p53 was found in cancer cells in 87.01%, 85.71%, 68.18%, 98.05%, 55.19% and 50.65% cases respectively. Linear regression and correlation analysis revealed that there was close positive relationship ( P <0.05) between the expression of MMP 2 and MMP 9, TIMP 1 and CD44v6, HER2/neu and MMP 9, MMP 2 and p53. Up regulation of MMP 2 was accompanied by advanced T stage ( P <0.01) . There was also a trend of MMP 2 expression being related with tumor metastasis. Increased expression of HER2/neu was found in patients with tumor recurrence( P <0.05). The expression of TIMP 1 was higher in laryngeal cancer than that in pharyngeal cancer, and higher in keratinizing and non keratinizing SCC than that in basaloid SCC( P <0.05). These findings suggested that MMP 2 and MMP 9, HER2/neu and MMP 9, MMP 2 and p53 had a coordinate function in aggression of tumor; that MMP 2 had a more important function than MMP 9 in tumor invasion and metastasis; and that HER2/neu might serve as a biomarker for poor prognosis in HNSCC.展开更多
Soy protein isolate/carboxymethyl chitosan （SPI/CMCH） blended films incorporated with glycerol were prepared using solution casting to investigate the effects of the SPI and CMCH ratios （100：0, 88：12, 67：33, 50...Soy protein isolate/carboxymethyl chitosan （SPI/CMCH） blended films incorporated with glycerol were prepared using solution casting to investigate the effects of the SPI and CMCH ratios （100：0, 88：12, 67：33, 50：50, 33：67, 12：88, 0：100） on the water sorption isotherm. The moisture sorption isotherm of the SPI/CMCH blended films was determined using various relative humidity＇s （16%, 35%, 55% and 76% RH） at 25 ± 1℃. The isotherms showed that the equilibrium moisture content （EMC） of the films increased with increasing CMCH content and the EMC value sharply increased above aw = 0.55. Understanding of sorption isotherms is important for prediction of moisture sorption properties of films via moisture sorption empirical models. The Guggenheim-Oswin, Brunauer-Emmett-Teller （BET）, and Anderson-de Boer （GAB） sorption model predictions were tested against the experimental data. The root mean square （RMS） values from the Oswin, BET, and GAB models respectively ranged from 698.54 to 1,557.54, 38.85 to 58.30, and 52.52 to 95.95. Therefore, the BET model was found to be the best-fit model for SPI/CMCH blended films at 25 ± 1 ℃.展开更多
文摘This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase 2, 9 (MMP 2, MMP 9), tissue inhibitor 1 of matrix metalloproteinase (TIMP 1), cell adhesion molecule 44 variant 6 (CD44v6), HER2/neu and p53 was investigated in 154 patients with head and neck squamous cell carcinoma (SCC) by ABC and ImmunoMax immunohistochemical method. Their clinical relevance and correlation were analysed. The expression of MMP 2, MMP 9, TIMP 1, CD44v6, HER2/neu and p53 was found in cancer cells in 87.01%, 85.71%, 68.18%, 98.05%, 55.19% and 50.65% cases respectively. Linear regression and correlation analysis revealed that there was close positive relationship ( P <0.05) between the expression of MMP 2 and MMP 9, TIMP 1 and CD44v6, HER2/neu and MMP 9, MMP 2 and p53. Up regulation of MMP 2 was accompanied by advanced T stage ( P <0.01) . There was also a trend of MMP 2 expression being related with tumor metastasis. Increased expression of HER2/neu was found in patients with tumor recurrence( P <0.05). The expression of TIMP 1 was higher in laryngeal cancer than that in pharyngeal cancer, and higher in keratinizing and non keratinizing SCC than that in basaloid SCC( P <0.05). These findings suggested that MMP 2 and MMP 9, HER2/neu and MMP 9, MMP 2 and p53 had a coordinate function in aggression of tumor; that MMP 2 had a more important function than MMP 9 in tumor invasion and metastasis; and that HER2/neu might serve as a biomarker for poor prognosis in HNSCC.
文摘Soy protein isolate/carboxymethyl chitosan （SPI/CMCH） blended films incorporated with glycerol were prepared using solution casting to investigate the effects of the SPI and CMCH ratios （100：0, 88：12, 67：33, 50：50, 33：67, 12：88, 0：100） on the water sorption isotherm. The moisture sorption isotherm of the SPI/CMCH blended films was determined using various relative humidity＇s （16%, 35%, 55% and 76% RH） at 25 ± 1℃. The isotherms showed that the equilibrium moisture content （EMC） of the films increased with increasing CMCH content and the EMC value sharply increased above aw = 0.55. Understanding of sorption isotherms is important for prediction of moisture sorption properties of films via moisture sorption empirical models. The Guggenheim-Oswin, Brunauer-Emmett-Teller （BET）, and Anderson-de Boer （GAB） sorption model predictions were tested against the experimental data. The root mean square （RMS） values from the Oswin, BET, and GAB models respectively ranged from 698.54 to 1,557.54, 38.85 to 58.30, and 52.52 to 95.95. Therefore, the BET model was found to be the best-fit model for SPI/CMCH blended films at 25 ± 1 ℃.
