[Objective] The aim was to improve part of auto-control system of aquatic seedling breeding and to lay foundation for highly-efficient and stable use of the sys- tem. [Method] The executive and drive modules of the au...[Objective] The aim was to improve part of auto-control system of aquatic seedling breeding and to lay foundation for highly-efficient and stable use of the sys- tem. [Method] The executive and drive modules of the auto-control system were im- proved, after which real-time monitor, data display and alarm modules were tested on time. [Result] In the test, for the improved system, stability and sensitivity were enhanced; different components matched better; transmitting electric resistanc6 was reduced. [Conclusion] The research provides references for auto-control of water temperature and dissolved oxygen, and faults clearing in aquatic seedling breeding.展开更多
Immune checkpoint inhibitors are increasingly drawing much attention in the therapeutic development for cancer treatment. However, many cancer patients do not respond to treatments with immune checkpoint inhibitors, p...Immune checkpoint inhibitors are increasingly drawing much attention in the therapeutic development for cancer treatment. However, many cancer patients do not respond to treatments with immune checkpoint inhibitors, partly because of the lack of tumor-infiltrating effector T cells. Cancer vaccines may prime patients for treatments with immune checkpoint inhibitors by inducing effector T-ceU infiltration into the tumors and immune checkpoint signals. The combination of cancer vaccine and an immune checkpoint inhibitor may function synergistically to induce more effective antitumor immune responses, and clinical trials to test the combination are currently ongoing.展开更多
AIM: To investigate the expression of several important molecules involved in major histocompatibility complex (MHC) class I presentation pathway in primary hepatocellular carcinoma (HCC), and to determine whether cyt...AIM: To investigate the expression of several important molecules involved in major histocompatibility complex (MHC) class I presentation pathway in primary hepatocellular carcinoma (HCC), and to determine whether cytotoxic T lymphocyte (CTL) vaccine therapy was suitable for HCC. METHODS: Labeled streptavidin biotin(LSAB) method of immunohisto-chemistry was used to study 33 HCC tissue specimens. RESULTS: Most HCC tissues and adjacent histological normal hepatocytes expressed HLA-I antigens,TAP,and B7, expression of B7 was especially strong,and there was no significant difference between them (P>0.05). CONCLUSION: The MHC class I presentation pathway in primary hepatocellular carcinoma may not be abnormal or dysfunctional,and CTL could kill these tumor cells. Thus, it is suitable and practicable to design and construct CTL vaccine against HCC.展开更多
Root-knot nematodes （Meloidogvne spp.） constrain the production of high value vegetable crops, such as tomato, on smallholder farms in Kenya. For several years, control of root-knot nematodes largely depended on the...Root-knot nematodes （Meloidogvne spp.） constrain the production of high value vegetable crops, such as tomato, on smallholder farms in Kenya. For several years, control of root-knot nematodes largely depended on the use of synthetic nematicides, most of which have been banned due to their toxicity to humans and the environment. Farmer-participatory trials were established, for two consecutive growing seasons on farmers＇ fields infested with root-knot nematodes （A4eloidogvne javanica and Meloidogyne incognita）, at two sites in central Kenya （Maragua and Kirinyaga districts）, representing two contrasting group of farmers （low-input and high-input）. The trials aimed at evaluating the potential of using biological control agents （BCAs）, i.e. Poehonia ehlamydosporia and Pasteuria penetrans, antagonistic plants （Crotalaria grahamiana and Tagetes minuta）, ＂＇trash burning＂ and chemical control （dazomet 98% = Basamid~ Granular） in the management of root-knot nematodes in tomato nursery beds, and quantify the associated net benefits, compared to an untreated control. Crotalaria grahamiana, ＂trash burning＂ and dazomet, significantly （P 〈 0.05） reduced the number of second-stage juveniles （J2s） of the root-knot nematodes in the soil in nursery beds during the first season at Karigui-ini, but there was no significant （P 〉 0.05） increase in yield after transplanting tomato seedlings from the respective treatment. Similarly, one of the BCAs （P. ehlamydosporia）, was effectively transferred to the field with seedlings from the BCA-treated nursery beds, but there was no significant （P 〉 0.05） increase in yield due to slow proliferation of the BCA, after transplanting. Participatory partial budgets were successfully developed with srnallholder farmers, and proved to be a vital tool in making informed decisions on management options for root-knot nematodes. In general, labour was considered by all farmers as the major input that contributed to the overall additional cost of the different treatments at both sites. This necessitates improvement in efficiency of labour resource use.展开更多
In this study, the efficacy of the native isolates of Trichoderma species to promote the growth and yield parameters of cucumber and to manage Fusarium wilt disease under greenhouse conditions were investigated. Ninet...In this study, the efficacy of the native isolates of Trichoderma species to promote the growth and yield parameters of cucumber and to manage Fusarium wilt disease under greenhouse conditions were investigated. Ninety native Trichoderma antagonists were isolated from Grassland and forest soil in different geographical regions of Inner Mongolia, China. Applications of T. cf. harzianum T1, T2, T3, T4, T5, T6, T7, TS, T10, T11 and T. atroviride （T9） exhibited the least disease incidence （by 0%） also the same strains shows 100% of relative control effect. Cucumber seedling treated with T. cf. harzianum （T2 and T1） isolates showed a significant stimulatory effect on plant height （by 13 and 14 cm respectively） and the highest shoot and root fresh weight were recorded by T. asperelloides （T27） and T. gamsii （T17） by 1.62 gm and 0.97 gm respectively, in comparison to untreated control and treated control （10 cm and 4 cm, 1.27 gm and 0.22 gm and 0.82 gm and 0.10 gm）. Therefore, the antagonist （T1, T2, T3, T4, T5, T6, T7, T8, T8, T10, and T1 1） is chosen to be the most promising bio-control agent for Fusarium oxysporum f. sp. Cucumerinum and further study have to be exploited for sustainable disease management program.展开更多
[Objective]The aim of this study was to explore allelopathic effects of the water-extracted crude of cucumber plant parts on seed germination and seedling growth of lettuce(Lactuca sativa L.),cabbage(Brassica pekinens...[Objective]The aim of this study was to explore allelopathic effects of the water-extracted crude of cucumber plant parts on seed germination and seedling growth of lettuce(Lactuca sativa L.),cabbage(Brassica pekinensis L.),radish(Raphanus sativus L.) and scallion(Allium fistulosum L.).[Method]The allelopathic effects of different cucumber tissues aqueous extracts were investigated on lettuce,cabbage,radish and scallion using the water-soluble allelopathic principle by seedling bioassay tests.[Result]The trends of inhibitory effects of different cucumber parts increased with increasing extract concentrations.The inhibition effects and the inhibition rate of three aqueous extracts were more significant on root growth than shoot length.Leaf aqueous extracts represented the most exhibited and root aqueous extracts representing the least inhibitory on seed germination,shoot length and root length of lettuce,cabbage,radish and scallion.[Conclusion]This study had provided basis for allelopathic character of different cucumber plant parts.展开更多
To construct and express the fusion protein Stx2B-IntiminC300 of EHEC O157 ： H7, and to further investigate its immunoprophylactic potential, the gene of Stx2B （stx2b） from EHEC O157：H7 chromosome was cloned into ...To construct and express the fusion protein Stx2B-IntiminC300 of EHEC O157 ： H7, and to further investigate its immunoprophylactic potential, the gene of Stx2B （stx2b） from EHEC O157：H7 chromosome was cloned into pMD18-T vector. Thereafter, the amplified gene was cloned into prokary- otic expression plasmid pET-28a （ ＋ ）-eaeC300, which was constructed previously. The recombinant pasmid pET-28a（ ＋ ）-stx2b-eaeC300 was transformed into E. coli BL21 （DE3）. After inducement, the protein Stx2B-IntiminC300 was successfully expressed and analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE）, Western blotting and N-terminal amino acid residual sequencing. To evaluate its immunoprophylactic potential, it was primarily purified by ion-exchange chromatography and injected into 30 BALB/c mice with AI（OH）3 in the subscapular region. Ten days after the last booster vaccination, 20 mice were attacked with EHEC O157：H7 lysate and the protective efficacy was observed. In the present study, the gene of Stx2B-intiminC300 was successfully cloned into pET-28a （ ＋ ） vector. The results of SDS-PAGE and Western blotting assay showed that the fusion protein was successfully expressed in the inclusion body form, accounting for 25 % of total expression products, and its molecular weight was about 43 kDa. The result of the N-terminal amino acid residual sequencing showed that it was identical to that of the molecular designed. The purity was about 75 % after primary purification. Animal tests revealed that the fusion protein Stx2B-intiminC300 has elicited high titer of protective antibody relatively. These results demonstrate that the fusion protein Stx2B-IntiminC300 is successfully expressed in prokaryotic expression system and shows certain immunoprophylactic potential.展开更多
文摘[Objective] The aim was to improve part of auto-control system of aquatic seedling breeding and to lay foundation for highly-efficient and stable use of the sys- tem. [Method] The executive and drive modules of the auto-control system were im- proved, after which real-time monitor, data display and alarm modules were tested on time. [Result] In the test, for the improved system, stability and sensitivity were enhanced; different components matched better; transmitting electric resistanc6 was reduced. [Conclusion] The research provides references for auto-control of water temperature and dissolved oxygen, and faults clearing in aquatic seedling breeding.
基金supported by the Viragh Foundation(L.Z.)National Institutes of Health(NIH)(Grant No.K23 CA148964,L.Z.)the NCI SPORE in Gastrointestinal Cancers(Grant No.P50 CA062924,L.Z.)
文摘Immune checkpoint inhibitors are increasingly drawing much attention in the therapeutic development for cancer treatment. However, many cancer patients do not respond to treatments with immune checkpoint inhibitors, partly because of the lack of tumor-infiltrating effector T cells. Cancer vaccines may prime patients for treatments with immune checkpoint inhibitors by inducing effector T-ceU infiltration into the tumors and immune checkpoint signals. The combination of cancer vaccine and an immune checkpoint inhibitor may function synergistically to induce more effective antitumor immune responses, and clinical trials to test the combination are currently ongoing.
基金Supported by the National Natural Science Foundation of China, No. 30070855
文摘AIM: To investigate the expression of several important molecules involved in major histocompatibility complex (MHC) class I presentation pathway in primary hepatocellular carcinoma (HCC), and to determine whether cytotoxic T lymphocyte (CTL) vaccine therapy was suitable for HCC. METHODS: Labeled streptavidin biotin(LSAB) method of immunohisto-chemistry was used to study 33 HCC tissue specimens. RESULTS: Most HCC tissues and adjacent histological normal hepatocytes expressed HLA-I antigens,TAP,and B7, expression of B7 was especially strong,and there was no significant difference between them (P>0.05). CONCLUSION: The MHC class I presentation pathway in primary hepatocellular carcinoma may not be abnormal or dysfunctional,and CTL could kill these tumor cells. Thus, it is suitable and practicable to design and construct CTL vaccine against HCC.
文摘Root-knot nematodes （Meloidogvne spp.） constrain the production of high value vegetable crops, such as tomato, on smallholder farms in Kenya. For several years, control of root-knot nematodes largely depended on the use of synthetic nematicides, most of which have been banned due to their toxicity to humans and the environment. Farmer-participatory trials were established, for two consecutive growing seasons on farmers＇ fields infested with root-knot nematodes （A4eloidogvne javanica and Meloidogyne incognita）, at two sites in central Kenya （Maragua and Kirinyaga districts）, representing two contrasting group of farmers （low-input and high-input）. The trials aimed at evaluating the potential of using biological control agents （BCAs）, i.e. Poehonia ehlamydosporia and Pasteuria penetrans, antagonistic plants （Crotalaria grahamiana and Tagetes minuta）, ＂＇trash burning＂ and chemical control （dazomet 98% = Basamid~ Granular） in the management of root-knot nematodes in tomato nursery beds, and quantify the associated net benefits, compared to an untreated control. Crotalaria grahamiana, ＂trash burning＂ and dazomet, significantly （P 〈 0.05） reduced the number of second-stage juveniles （J2s） of the root-knot nematodes in the soil in nursery beds during the first season at Karigui-ini, but there was no significant （P 〉 0.05） increase in yield after transplanting tomato seedlings from the respective treatment. Similarly, one of the BCAs （P. ehlamydosporia）, was effectively transferred to the field with seedlings from the BCA-treated nursery beds, but there was no significant （P 〉 0.05） increase in yield due to slow proliferation of the BCA, after transplanting. Participatory partial budgets were successfully developed with srnallholder farmers, and proved to be a vital tool in making informed decisions on management options for root-knot nematodes. In general, labour was considered by all farmers as the major input that contributed to the overall additional cost of the different treatments at both sites. This necessitates improvement in efficiency of labour resource use.
文摘In this study, the efficacy of the native isolates of Trichoderma species to promote the growth and yield parameters of cucumber and to manage Fusarium wilt disease under greenhouse conditions were investigated. Ninety native Trichoderma antagonists were isolated from Grassland and forest soil in different geographical regions of Inner Mongolia, China. Applications of T. cf. harzianum T1, T2, T3, T4, T5, T6, T7, TS, T10, T11 and T. atroviride （T9） exhibited the least disease incidence （by 0%） also the same strains shows 100% of relative control effect. Cucumber seedling treated with T. cf. harzianum （T2 and T1） isolates showed a significant stimulatory effect on plant height （by 13 and 14 cm respectively） and the highest shoot and root fresh weight were recorded by T. asperelloides （T27） and T. gamsii （T17） by 1.62 gm and 0.97 gm respectively, in comparison to untreated control and treated control （10 cm and 4 cm, 1.27 gm and 0.22 gm and 0.82 gm and 0.10 gm）. Therefore, the antagonist （T1, T2, T3, T4, T5, T6, T7, T8, T8, T10, and T1 1） is chosen to be the most promising bio-control agent for Fusarium oxysporum f. sp. Cucumerinum and further study have to be exploited for sustainable disease management program.
基金Supported by the Natural Science Foundation of Beijing(6091001)National nature Foundation of Science(30972034)National Science and Technology Support Plan(2011BAD12B03)
文摘[Objective]The aim of this study was to explore allelopathic effects of the water-extracted crude of cucumber plant parts on seed germination and seedling growth of lettuce(Lactuca sativa L.),cabbage(Brassica pekinensis L.),radish(Raphanus sativus L.) and scallion(Allium fistulosum L.).[Method]The allelopathic effects of different cucumber tissues aqueous extracts were investigated on lettuce,cabbage,radish and scallion using the water-soluble allelopathic principle by seedling bioassay tests.[Result]The trends of inhibitory effects of different cucumber parts increased with increasing extract concentrations.The inhibition effects and the inhibition rate of three aqueous extracts were more significant on root growth than shoot length.Leaf aqueous extracts represented the most exhibited and root aqueous extracts representing the least inhibitory on seed germination,shoot length and root length of lettuce,cabbage,radish and scallion.[Conclusion]This study had provided basis for allelopathic character of different cucumber plant parts.
文摘To construct and express the fusion protein Stx2B-IntiminC300 of EHEC O157 ： H7, and to further investigate its immunoprophylactic potential, the gene of Stx2B （stx2b） from EHEC O157：H7 chromosome was cloned into pMD18-T vector. Thereafter, the amplified gene was cloned into prokary- otic expression plasmid pET-28a （ ＋ ）-eaeC300, which was constructed previously. The recombinant pasmid pET-28a（ ＋ ）-stx2b-eaeC300 was transformed into E. coli BL21 （DE3）. After inducement, the protein Stx2B-IntiminC300 was successfully expressed and analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE）, Western blotting and N-terminal amino acid residual sequencing. To evaluate its immunoprophylactic potential, it was primarily purified by ion-exchange chromatography and injected into 30 BALB/c mice with AI（OH）3 in the subscapular region. Ten days after the last booster vaccination, 20 mice were attacked with EHEC O157：H7 lysate and the protective efficacy was observed. In the present study, the gene of Stx2B-intiminC300 was successfully cloned into pET-28a （ ＋ ） vector. The results of SDS-PAGE and Western blotting assay showed that the fusion protein was successfully expressed in the inclusion body form, accounting for 25 % of total expression products, and its molecular weight was about 43 kDa. The result of the N-terminal amino acid residual sequencing showed that it was identical to that of the molecular designed. The purity was about 75 % after primary purification. Animal tests revealed that the fusion protein Stx2B-intiminC300 has elicited high titer of protective antibody relatively. These results demonstrate that the fusion protein Stx2B-IntiminC300 is successfully expressed in prokaryotic expression system and shows certain immunoprophylactic potential.
