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农杆菌介导洋桔梗遗传转化影响因素的研究 被引量:4
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作者 张娟 曹伟杰 +3 位作者 陈崇顺 马文晔 王转梅 岳敏 《江苏农业科学》 CSCD 北大核心 2010年第2期23-25,共3页
以洋桔梗品种Double Mariachi Pink叶片为外植体,研究了影响农杆菌介导遗传转化效率的几个重要因素。结果表明,叶片外植体与农杆菌合适的共培养时间为5 d;以新的切割方法——"刺孔法"切割叶片,每个叶块外植体再生不定芽平均15... 以洋桔梗品种Double Mariachi Pink叶片为外植体,研究了影响农杆菌介导遗传转化效率的几个重要因素。结果表明,叶片外植体与农杆菌合适的共培养时间为5 d;以新的切割方法——"刺孔法"切割叶片,每个叶块外植体再生不定芽平均15.22个,明显多于"三刀切"的11个;"刺孔法"的叶块转化率达到77.7%,每个叶块再生卡那霉素抗性苗平均0.64株,明显高于"三刀切"的57.7%和0.38株;在"刺孔法"处理中,与孔外边缘切口相比,分布均匀的孔内切口利于更多不定芽的再生和卡那霉素抗性苗的形成。 展开更多
关键词 洋桔梗 农杆菌介导 遗传转化 共培养 刺孔法
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CLINICAL STUDY ON TREATMENT OF TRIGEMINAL NEURALGIA BY NEEDLING MENTAL AND MANDIBULAR FORAMINA
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作者 董宇翔 韩春霞 张二力 《World Journal of Acupuncture-Moxibustion》 2001年第1期33-35,共3页
In the present paper, 126 patients with neuralgia involving the mandibular branch of trigeminal nerve were treated mainly by puncturing the mental foramen and the mandibular foramen. The two foramina are both located ... In the present paper, 126 patients with neuralgia involving the mandibular branch of trigeminal nerve were treated mainly by puncturing the mental foramen and the mandibular foramen. The two foramina are both located in the focus through which the Stomach Channel of Foot Yangming passes. Among the 126 patients, 42 were attributed to the light type, 61 to the moderate type one and the other 23 to serious type. Acupuncture treatment was given once daily, 30 min every time, with 10 days being a therapeutic course. After 3 courses of treatment, results showed that 29 (69.1%) light type cases, 35(57.4%) moderate type cases and 6 (26.1%) serious type cases were cured. It displays that the less serious the symptom, the higher is the cure rate, and the shorter the duration of disease, the better is the therapeutic effect. 展开更多
关键词 Acupuncture therapy Mental and mandibular foramina Trigeminal neuralgia
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