益气活血利水汤配合脏时相调法针刺治疗宫颈癌术后尿潴留的临床疗效分析

[目的]探讨益气活血利水汤配合脏时相调法针刺治疗宫颈癌术后尿潴留的临床疗效。[方法] 2021年12月—2022年12月纳入112例宫颈癌术后尿潴留患者,按照随机数字表法将患者分为两组,每组各56例。两组均给予留置导尿、物理刺激训练膀胱功能,在此基础上,对照组采用益气活血利水汤治疗,观察组采用益气活血利水汤联合脏时相调法针刺治疗。比较两组疗效、中医证候积分、恢复自主排尿时间、平均每次排尿时间、尿动力学、膀胱过度活动综合征评分(OABSS)和不良反应差异。[结果]观察组治疗总有效率高于对照组(96.343%vs. 80.36%,P<0.05),恢复自主排尿时间、平均每次排尿时间均短于对照组(P<0.05)。治疗后两组小便短少、面色淡白、疲倦无力、四肢不温、心慌失眠、胸胁疼痛证候积分以及膀胱最大容量、最大尿流率、逼尿肌压力、残余尿量、OABSS评分较治疗前降低(P<0.05),且观察组治疗后上述中医证候积分以及膀胱最大容量、最大尿流率、逼尿肌压力、残余尿量、OABSS评分低于对照组(P<0.05)。[结论]益气活血利水汤配合脏时相调法针刺可显著改善膀胱功能,提高临床疗效且安全性高。

择时穴位刺血治疗胃(肠)火牙痛的疗效观察

目的观察按子午流注择时取穴刺血治疗胃(肠)火牙痛的疗效。方法将76例符合胃(肠)火牙痛的患者分为治疗组和对照组各38例,治疗组用子午流注纳子法于辰时(胃火)、卯时(肠火)刺血治疗,对照组则服用黄连解毒片。结果 1个疗程后治疗组总有效率为92.11%,对照组总有效率为68.42%,两组总有效率比较,差异有统计学意义(χ2=6.73,P﹤0.01)。结论用子午流注择时取穴刺血治疗胃(肠)火牙痛疗效确切,且无副作用,值得推广应用。

“为觅出人句,只求当路知”——诗人曹松的诗歌创作

唐代诗人曹松一生交游甚众,和众多的诗人有着唱和,但仕途坎坷,颠沛流离;而作为封建时代的知识分子,却又关心民间疾苦,厌恶战争;身为唐代才子,创作甚丰,留给后人一百多首诗歌,亦颇见功力。

《氓》释义综述

《氓》是我国古代一篇优秀的叙事诗,古往今来许多学者对此诗有过分析、评价,不同的学者有着不同的看法,概括起来有先秦《诗》学、《诗经》汉学、《诗经》宋学、《诗经》清学和现代《诗学》五个阶段。

EXPERIMENTAL STUDY ON THE BLOOD BIOCHEMICAL PARAMETERS FOR THE PROMOTION OF FRACTURE HEALING BY MS

This paper describes an experimental study about magnetic stimulation (MS) effects on the fracture healing of 120 fresh fractures and their blood biochemical parameters during their recovery. The mechanism of promoting the recovering process by MS is discussed and analysed. The experiments show that MS can advance calcium deposit on fractured bones and metabolism of calcium and phosphorus, which promote the process of fracture healing.

Development and Application of a Tyrosinase-Based Time-Temperature Indicator(TTI) for Determining the Quality of Turbot Sashimi

Time-temperature indicators(TTIs) are convenient intuitive devices that are widely used to predict food quality. The aim of this study is to develop a new simple device which can be attached to food packages as a quality indicator for turbot sashimi. In this study, a solid TTI based on the reaction between tyrosinase and tyrosine was developed. The Arrhenius behavior of this enzymatic TTI was studied. The kinetics of the tyrosinase-based TTI was investigated in the form of color change from colorless to dark black induced by the enzymatic reaction. The mathematical formula for the color alterations as a function of time and temperature was established. The longest indication time for the developed TTI was 50 hours at 4℃. The activation energy of the tyrosinase-based TTI was 0.409 k J mol^(-1). The suitability of the tyrosinase-based TTI was validated for turbot sashimi using total plate count. The feasibility of using this TTI as a quality indicator for turbot sashimi was assessed based on the activation energy and indication time. Therefore, the tyrosinasebased TTI system developed in this study could be used as an effective tool for monitoring the quality changes of turbot sashimi during the distribution and storage.

CLINICAL OBSERVATION ON TREATMENT OF STUBBORN HICCUP BY ACUPUNCTURE WITH DIFFERENT NEEDLE RETAINING TIME

Purpose:Observe the effect of different duration of needle-retention on acupuncture treatment of stubborn hiccup. Methods: 85 cases of stubborn hiccup patients are randomly divided into control group (n=40) and treatment group(n=45) according to their visiting time. For patients of control group, acupuncture needles (in both scalp points and body acupoints) are retained for 30 min, while for patients of treatment group, the needles inserted in the body acupoints are retained for 1 hr, and that inserted in scalp-point is retained for more than 6 hrs. The treatment is conducted once daily, continuously for 5 times. Results: The cure rate of treatment group is 88.9% (that for deficiency syndrome is 85.3%), and that of control group is 65% (that for deficiency syndrome is 53.3%).There is a significant difference between two groups in the cure rate (P<0.05). The cure rates for excess syndrome of two groups both are 100%.Conclusion: Longer retention of acupuncture needle has a better therapeutic effect for deficiency-syndrome type hiccup.

LEPTIN STIMULATING PROLIFERATION AND DIFFERENTI-ATION OF HUMAN OSTEOBLAST-LIKE CELL LINE MG63 IN DOSE-DEPENDENT AND TIME-DEPENDENT MANNERS

Objective To investigate the direct effect of leptin on osteoblast-like cell line MG63. Methods Human osteoblast-like cell line MG63 was incubated with leptin of different doses for 24, 48 and 72 h, respectively. The proliferation of MG63 was determined by methylene blue assay. Alphal （1） collagen gene expression in MG63 was determined by real time flourescenee quantitive PCR （FQ-PCR）, both with 17β-E2 as positive control. Results Leptin accelerated the proliferation and differentiation of MG63 in dose and time-dependent manners, with the best effect at 10^-7 mol/L at 72 h. Compared with 17β-E2, leptin showed a weaker promoting effect at all of the three time point： 24, 48 and 72 h. While the effects of the two hormones have an approaching trend the time prolonged. Conclusion Leptin has the effects of accelerating the proliferation and differentiation of MG63 cells in vitro, which is more enduring and later than that of 17β-E2.

INFLUENCE OF DIFFERENT NEEDLE-RETAINING DURATION ON CEREBRAL HEMODYNAMICS OF CEREBRAL PARALYSIS CHILDREN

Objective: To investigate the effects of different needle-retaining duration on cerebral hemodynamics in cerebral paralysis (CP) children. Methods: A total of 20 cases of CP children were subjected into this study. Changes of systolic peak value (Vs), blood flow velocity at the end-diastolic phase (Ved), mean velocity (Vm), pulsation index (PI) and resistance index (RI) of the cerebral hemodynamics before and after performing JIN’s Three-Needling Therapy with the needles retained for 30 min (group A) and 5 min (group B) were observed by using transcranial Doppler ultrasonography (TCD). Results: After acupuncture, the blood flow in the middle cerebral artery (MCA), anterior cerebral artery (ACA) and posterior cerebral artery (PCA) was accelerated, and the resistance of blood vessels decreased. The effect of needle-retaining-30 min was better than that of needle-retaining-5 min. Conclusion: In treating cerebral paralysis by using JIN’s Three-Needling Therapy, sufficient stimulation provided by needle remaining is an important factor for achieving satisfactory therapeutic effects.

EFFECT OF ACUPUNCTURE OF "ZUSANLI" AND "WEISHU" ACUPOINTS ON EXPERIMENTAL GASTRIC ULCER

Objective: To observe the therapeutic effect of electroacupuncture (EA) of "Zusanli"(ST 36) and "Weishu"(BL 21)on the experimental gastric ulcer in Xu period of a day for analyzing the best opportunity of acupuncture treatment of gastric ulcer. Methods:49 Wistar rats were randomly divided into ① control group, ② ST 36 Xu group, ③ BL 21 Xu group, ④ ST 36 Chen group, ⑤ BL 21 Chen group, ⑥ ST 36 IT group, and ⑦ BL 21 IT group, with 7 rats in each group. Rat gastric ulcer model was established using intra gastric administration of glacial acetic acid. Gastric mucosal thickness,mucosal muscular defect width,superficial mucosal mucus index (MI),intramucosal MI ,Total MI,plasma 6 keto PGF1α ,serum NO and gastric tissue CGRP concentrations were used as the indexes. The EA treatment was conducted once daily at Xu period (7-9 o’clock in the evening), Chen period (8-10 o’clock in the morning) and irregular time (IT) with 6 days being a therapeutic course, two courses altogether. Results:After EA treatment,①the regenerated mucosal thickness values of the aforementioned 6 EA groups, particularly ST 36 Xu group and BL 21 Xu group, were all apparently higher than that of control group (P<0.05-0.01), while the mucosal muscle layer defect width values of different EA groups, particularly ST 36 Xu group and BL 21 Xu group, were strikingly lower than that of control group (P<0.05-0.01); ②the 3 MI of the 6 EA groups, particularly those of ST 36 Xu group were all significantly higher than those of control group (P<0.05-0.01); ③plasma 6 keto PGF1α contents of the 6 EA groups, particularly those of ST 36 Xu and BL 21 Xu groups, were all significantly higher than that of control group (P<0.05-0.01); ④serum NO and gastric tissue CGRP concentrations of the 6 EA groups, particularly those of ST 36 Xu and BL 21 Xu groups and CGRP concentration of ST 36 Chen group, were all significantly higher than those of control group (P<0.05-0.01);and ⑤there were positive correlation between superficial MI or total MI and NO level (r=0.858, 0.987, P<0.05,and 0.01),and between the superficial mucus index and CGRP concentration (r=0.9051, t=4.68, P<0.01), suggesting that the increase of the secretion function of the regenerated mucosal mucus is related to acupuncture induced increase of release of both NO and CGRP.Conclusion:The therapeutic effect of EA of ST 36 and BL 21 in Xu period is better in the treatment of gastric ulcer,and that of EA of ST 36 is the best.

Timeliness of the analgesic effect of superficial needling on shoulder-hand syndrome after stroke

Objective To observe the correlation between analgesic effect and duration of analgesic effect of superficial needling for shoulder pain of shoulderhand syndrome（SHS） after stroke, so as to screen the best time period of analgesia. Methods A total of 120 patients with SHS after stroke（stage I） were recruited and superficial needling therapy was applied. Two obvious tenderness points on the affected shoulder of patients were found out. The site 80–100 mm down each tenderness point was selected for superficial needling. Bimanual needling technique was applied after inserting needles. The surrounding of tenderness points was pinched and grasped by left hand above the needling, and the technique of green dragon swaying tail was applied by right hand. The needles at each acupoint were manipulated for3 min and retained for 30 min. The analgesic effect was evaluated dynamically by visual analogue scale（VAS） in 120 patients before treatment, immediately after treatment, 30 min after treatment, 1 h after treatment and 24 h after treatment. The analgesic effects at different time were statistically analyzed by generalized estimating equation. Results The mean values of VAS were 7.483, 3.950, 4.767, 5.917 and 7.217, respectively, before treatment, immediately after treatment, 30 min after treatment, 1 h after treatment and 24 h after treatment. The difference of analgesic effect at different time was statistically significant（P〈0.01）; the difference of analgesic effect of superficial needling in treatment of SHS after stroke was significant between immediately after treatment and 30 min after treatment（both P〈0.05）, while there was no significant difference between 1 h after treatment and 24 h after treatment（both P〈0.05）. Conclusion Analgesic effect of superficial needling for shoulder pain of SHS after stroke was different at different time points and decreased over time; analgesic effect was the most significant immediately after treatment and the optimal duration of analgesic effect was from immediately to 30 min after superficial needling therapy.

Optimization of acupuncture treatment programs for facial paralysis

Objective To optimize acupuncture treatment programs for facial paralysis.Methods Sixty-three cases of facial paralysis were randomly grouped according to orthogonal design L9（3）4 table,and treated by different combined programs of 4 factors,A（acupuncture opportunity）,B（combination of points）,C（stimulating quantity）,D（electroacupuncture time） and 3 levels,and changes of the functional score of the facial nerve were observed,and the different combined programs of the 4 factors,A（acupuncture opportunity）,B（combination of point）,C（stimulating quantity）,D（electroacupuncture time） and 3 levels influencing acupuncture therapeutic effect on facial paralysis were optimized.Results B（combination of points） and D（electroacupuncture time） were obvious factors（P0.05）,among them,B（combination of points） was the most main influencing factor;among the 3 levels of B（combination of points） and D（electroacupuncture time）,B3 （alternately needling two groups of acupoints） and D3 （electroacupuncture treatment at sparse-dense wave for 30 min） were the best.Conclusion Electroacupuncture at sparse-dense wave for 30 min,alternately needling two groups of acupoints is the best program for treatment of facial paralysis.

Analysis of evidence-based clinical practices on timing factor in acupuncture for facial paralysis

Objective By using the evidence-based medicine approach to analyze the optimum timing in acupuncture treatment for facial paralysis, to provide the scientific evidence for acupuncture clinical practices. Methods Computer searches were conducted in the CNKI literature database from January 1994 to August 2009, using the keywords ＂facial paralysis acupuncture＂ and ＂treating opportunity/acupuncture opportunity＂. According to the standard of Cochrane, the literatures were retrieved and conducted with evidence-based analysis. The SPSS 13.0 software was used for the statistical analysis. Results Among the 133 retrieved literatures which met the inclusion criteria of the study, there were 11 literatures on the effect of acupuncture or electroacupuncture treatment in acute stage observation group and conventional medical treatment group. Using Mann-Whitney U test, the difference of treatment effect is statistically significant （P〈0.001）. Conclusion Early intervention in acupuncture treatment for facial paralysis in acute stage can improve the efficacy and promote neurological recovery. In the acute stage, animal experiments and clinical observations provide evidence to support the application of electric needle and traditional acupuncture treatments.

Advance in study on influence factors of needle retention duration

Needle retention duration, as an essential link in acupuncture treatment, has always been valued by physicians in all ages of the Chinese history. In this article, related literatures in recent years on influence factors of needle retention duration have been collected and reviewed in terms of duration, type and state of illness, point selection and so on. Modern related researches, to a certain extent, have approved and advanced the viewpoints of the previous physicians. However, few of the studies are randomized controlled trials of evidence-based medicine. Most of them still remain in observation stage. Therefore, scientific designing methods should be adopted in the future for researching approaches of TCM. Systematic and through studies should be carried out on needle retention duration, so as to comprehend the principle and define the optimal duration for different diseases. In this way, a standardized and systematic model on acupuncture treatment can be established.

Clinical Study on Time-effect Relation of Acupuncture for Pain due to Dysmenorrhea

Objective： To observe the time-effect relationship of needling a single point or multiple points for pain relief in patients with severe primary dysmenorrhea. Methods： A total of 20 cases with severe primary dysmenorrhea were randomly allocated into a group of single point. In each treatment, the needles retained for 30 min and 15 person times were observed in each group. The VAS （Visual Analogue Scale） values were recorded immediately before needling, 5 min, 20 min and 30 min upon insertion, and 30 min, 60 min and 120 min upon withdrawal for statistical analysis. Results： Immediate pain relief occurred following acupuncture and the pain-alleviating effect was incremented within the 30 min of needle retaining. At 20 min upon needle insertion, the pain relief from needling multiple points appeared substantially stronger than needling a single point （P〈0.05） and this effect remained until 2 h after withdraw of the needles. Conclusion： Needling a single point or multiple points can both produce remarkably immediate pain relief for patients with severe primary dysmenorrhea and share similar time-effect relationship. However, needling multiple points showed substantially better effects than needling a single point in a number of time points.

Magnetism and Acupuncture

Qi as well as its ability to cure ailments can be released without needling by the application of magnetism. Qi-effects are based upon the magnetic force released by magnetic platelets fastened onto acupoints. This property supports and proves one of the definitions of qi which says, that ＂qi is bio-electrical magnetism＂ or ＂qi is defined as any type of energy which is able to demonstrate power and strength. This energy can be electricity, magnetism, heat, or light＂. Furthermore, it is suggested to use magnet acupuncture parallel to needle acupuncture according to traditional Chinese body acupuncture rules scientifically controlled in order to carry out research on qi and qi-effects. Here it might even be possible to find a quality and even a parameter to measure the intensity of qi-effects, such as by simply measuring the possible change of the heart beat frequency （pulse frequency） before and after application of the needle or magnetic platelet. It is also possible that there is another parameter in existence which will be changed by the impact of qi and acupuncture which still needs to be detected.

Promotion of Tissue Regeneration with Acupuncture

Acupuncture can promote the regeneration of tissues. That acupuncture therapy can be called “Regenerative Acupuncture”, which is based upon the idea that by the unlimited and universal power of qi the process of creation can be triggered again, disabled or destroyed physiological functions of organs and tissues can be restored, and even the reconstruction and renewal of a physically disabled or destroyed organ or tissue might be possible. Regenerative Acupuncture, its point selection and its rationale are based on the ancient and modem Chinese acupuncture literature and clinical experiences of the authors. Now this acupuncture method was introduced by one case report.

Efficacy observation of acupuncture for dry eye syndrome of lung-yin deficiency pattern

Objective:To observe the clinical efficacy of combining two needling manipulations,Er Long Xi Zhu(two dragons playing with a pearl)and Guo Yan Re(heat produced to reach the eyes),in treating dry eye syndrome(DES)of lung-yin deficiency pattern.Methods:Fifty-six eligible DES patients of lung-yin deficiency were randomized into an observation group and a control group,with 28 cases in each group.Same acupoints were selected in the two groups:Cuanzhu(BL 2),Sizhukong(TE 23),Taiyang(EX-HN 5)and Fengchi(GB 20)were chosen as the major points and Feishu(BL 13)and Chize(LU 5)as the adjuvant.Er Long Xi Zhu and Guo Yan Re needling manipulations were applied in the observation group while twirling reinforcing manipulation was used in the control group.Treatment was conducted once per day in both groups,for two sets of 15 consecutive days at a 2-day interval.Changes in the symptom score,tear break-up time(BUT)and tear production were observed afterwards,and the clinical efficacy was also compared between the two groups.Results:The total effective rate was 92.8%in the observation group,higher than 71.4%in the control group,and the between-group difference was statistically significant(P<0.05).After treatment,the symptom score,tear BUT and tear production showed significant improvements in both groups(all P<0.05);the symptom score,BUT and tear production in the observation group were significantly different from those in the control group(all P<0.05).Conclusion:Given the same acupoint selection,combining Er Long Xi Zhu and Guo Yan Re needling manipulations can produce more significant clinical efficacy than twirling reinforcing manipulation in treating DES of lung-yin deficiency pattern.

Mind-tranquilizing Needling Technique & Insomnia:Therapeutic Efficacy Observation of 63 Cases

Objective： To investigate the clinical efficacy of treating insomnia with mind-tranquilizing acupuncture. Method： Sixty three patients were randomly divided into a treatment group of 32 cases and a control group of 31 cases. The treatment group received mind-tranquilizing acupuncture and the control group, oral depression relieving and mind-calming herbal granules, for 30 days. Results： The response rate was 87.5% in the treatment group and 77.4% in the control group. There were significant differences in falling-sleep time, lasting period and times of nocturnal waking （P 〈 0.05） and a very significant difference in dream improvement （P〈 0.01） between the two groups. There were no significant differences in afterwaking conditions and sleep evaluation （P〉 0.05）. Conclusion： Clinically mind tranquilizing acupuncture has a better effect on insomnia than depression-relieving and mind-calming herbal granules.

Fracture union in percutaneous Kirschner wire fixation in paediatric tibial shaft fractures

Purpose： To evaluate the efficacy of intramedullary Kirschner wires for the treatment of unstable tibial shaft fractures in children. Methods： This prospective study was conducted at the Department of Orthopaedic Surgery in Maharishi Markandeshwar Medical College from June 2005 to June 2010. Sixty-six children having closed fracture of the tibial shaft with a mean age of 7.7 years （range, 2-14 years） were recruited from emergency and outpatient department. They were treated with percutaneous intramedullary Kirschner wires. The clinical results of our study were rated on the basis of the criteria of union, nonunion, delayed union or malunion. All children were followed for one year. Results： Children achieved union in a mean time of 8 weeks （range, 6-10 weeks）. Postoperatively, three children （4.55%） had delayed union, one （1.52%） valgus deformity of lower leg, three （4.55%） post- operative knee pain and twelve （18.18%） skin irritation at pin site. Wires were removed after 8-22 weeks without any complications. No patient was lost to follow-up. The results were excellent in 95.45% and good in 4.55% children. Conclusion： This technique is cost-effective, simple, quick to perform, safe and reliable and avoids pro- longed hospitalization with good results.