目的研究刺络泻血疗法对肝纤维化大鼠透明质酸(HA)、层粘连蛋白(LN)及肝组织病理学的影响,探讨其抗肝纤维化的疗效及作用机制。方法选用雄性SD大鼠40只,随机分为正常组、模型组、刺络泻血组(刺血组)、药物组4组。除正常组外,其余3组分...目的研究刺络泻血疗法对肝纤维化大鼠透明质酸(HA)、层粘连蛋白(LN)及肝组织病理学的影响,探讨其抗肝纤维化的疗效及作用机制。方法选用雄性SD大鼠40只,随机分为正常组、模型组、刺络泻血组(刺血组)、药物组4组。除正常组外,其余3组分别颈后皮下注射40%CCL4(花生油稀释)诱导肝纤维化模型。第6周起,刺血组从大鼠后肢肝经浅表静脉处刺络泻血,每次0.5 m L,每周2次;药物组给予秋水仙碱灌胃,每日200μg/kg体重,每周灌胃5次,至12周。第12周末,以10%水合氯醛麻醉大鼠,腹主动脉取血5 m L,进行丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和肝纤维指标HA、LN检测,剖取肝叶行Masson染色观察肝脏病理形态学改变。结果与正常组比较,模型组大鼠ALT、AST、HA、LN含量均升高,差异有统计学意义(P<0.05);与模型组比较,刺血组和药物组大鼠ALT、AST、HA、LN含量均降低,差异有统计学意义(P<0.05);与药物组比较,刺血组ALT、AST、HA、LN水平均有降低,但差异无统计学意义(P>0.05)。模型组Masson染色示肝脏纤维化明显,刺血组和药物组Masson染色肝纤维化程度较轻。结论刺络泻血疗法能减少肝纤维组织增生,阻止肝纤维化的发展,可能与下调大鼠血清细胞外基质HA、LN水平有关。展开更多
Objective To observe the analgesic effect on herpes zoster treated by the comprehensive therapy of blocking, surrounding needling, pricking and cupping combined with bloodletting at Longyan （龙眼, Extra） acupoint. M...Objective To observe the analgesic effect on herpes zoster treated by the comprehensive therapy of blocking, surrounding needling, pricking and cupping combined with bloodletting at Longyan （龙眼, Extra） acupoint. Methods According to the random mumber table, 104 cases of herpes zoster were randomized into an observation group and a control group, 52 cases in each one. In the observation group, the blocking method was used at the starting site and the ending site of herpetic zone, the surrounding needling method was applied toward the center of the painful zone, with horizontal needling technique; and the local pricking and cupping methods as well as the bloodletting at Longyan （Extra） acupoint were adopted. In the control group, Jiáj （夹脊 EX-B 2） at the corresponding affected nerve segments and the local Ashi points were selected and stimulated with electroacupuncture. The visual analogue scale （VAS） was adopted to determine the analgesic effect 20 min, 1, 6, 12, 24 and 48 h after treatment separately in each group. Results The onset time of analgesic effect in the observation group was shorter than that in the control group and the efficacy time of duration was longer than that in the control group （all P0.05）. The analgesic effect and the result of comprehensive efficacy assessment were superior to those of the control group （both P0.05）. Conclusion The comprehensive therapy of blocking, surrounding needling, pricking and cupping method combined with bloodletting at Longyan （Extra） achieves the significant analgesic effect on herpes zoster.展开更多
Objective： To observe the clinical efficacy of acupuncture plus bloodletting therapy in treating insomnia in people with blood stasis constitution. Methods： Seventy-two patients were randomized into a treatment grou...Objective： To observe the clinical efficacy of acupuncture plus bloodletting therapy in treating insomnia in people with blood stasis constitution. Methods： Seventy-two patients were randomized into a treatment group and a control group by using the random number table, 36 cases each. The treatment group received acupuncture plus bloodletting therapy, while the control group was intervened by oral administration of estazolam. One-week treatment was taken as a course, for 3 successive courses in total. A follow-up study was conducted 3 months later. The Pittsburgh sleep quality index （PSQI） was evaluated before and after the intervention, as well as in the follow-up. The clinical efficacies were also compared. Results： The total effective rate was 88.9% in the treatment group versus 83.3% in the control group, and the between-group difference was statistically significant （P〈0.O5）. After the intervention, the PSO.I scores dropped significantly in both groups （both P〈0.05）; the between-group difference in PSQI score was statistically significant （P〈O.05）. The follow-up study showed that the between-group difference in the global score of PSO, I was statistically significant （P〈0.05）. Conclusion： Acupuncture plus bloodletting therapy can produce a more significant efficacy than oral administration of estazolam in treating insomnia in people with blood stasis constitution.展开更多
文摘目的研究刺络泻血疗法对肝纤维化大鼠透明质酸(HA)、层粘连蛋白(LN)及肝组织病理学的影响,探讨其抗肝纤维化的疗效及作用机制。方法选用雄性SD大鼠40只,随机分为正常组、模型组、刺络泻血组(刺血组)、药物组4组。除正常组外,其余3组分别颈后皮下注射40%CCL4(花生油稀释)诱导肝纤维化模型。第6周起,刺血组从大鼠后肢肝经浅表静脉处刺络泻血,每次0.5 m L,每周2次;药物组给予秋水仙碱灌胃,每日200μg/kg体重,每周灌胃5次,至12周。第12周末,以10%水合氯醛麻醉大鼠,腹主动脉取血5 m L,进行丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和肝纤维指标HA、LN检测,剖取肝叶行Masson染色观察肝脏病理形态学改变。结果与正常组比较,模型组大鼠ALT、AST、HA、LN含量均升高,差异有统计学意义(P<0.05);与模型组比较,刺血组和药物组大鼠ALT、AST、HA、LN含量均降低,差异有统计学意义(P<0.05);与药物组比较,刺血组ALT、AST、HA、LN水平均有降低,但差异无统计学意义(P>0.05)。模型组Masson染色示肝脏纤维化明显,刺血组和药物组Masson染色肝纤维化程度较轻。结论刺络泻血疗法能减少肝纤维组织增生,阻止肝纤维化的发展,可能与下调大鼠血清细胞外基质HA、LN水平有关。
文摘Objective To observe the analgesic effect on herpes zoster treated by the comprehensive therapy of blocking, surrounding needling, pricking and cupping combined with bloodletting at Longyan （龙眼, Extra） acupoint. Methods According to the random mumber table, 104 cases of herpes zoster were randomized into an observation group and a control group, 52 cases in each one. In the observation group, the blocking method was used at the starting site and the ending site of herpetic zone, the surrounding needling method was applied toward the center of the painful zone, with horizontal needling technique; and the local pricking and cupping methods as well as the bloodletting at Longyan （Extra） acupoint were adopted. In the control group, Jiáj （夹脊 EX-B 2） at the corresponding affected nerve segments and the local Ashi points were selected and stimulated with electroacupuncture. The visual analogue scale （VAS） was adopted to determine the analgesic effect 20 min, 1, 6, 12, 24 and 48 h after treatment separately in each group. Results The onset time of analgesic effect in the observation group was shorter than that in the control group and the efficacy time of duration was longer than that in the control group （all P0.05）. The analgesic effect and the result of comprehensive efficacy assessment were superior to those of the control group （both P0.05）. Conclusion The comprehensive therapy of blocking, surrounding needling, pricking and cupping method combined with bloodletting at Longyan （Extra） achieves the significant analgesic effect on herpes zoster.
文摘Objective： To observe the clinical efficacy of acupuncture plus bloodletting therapy in treating insomnia in people with blood stasis constitution. Methods： Seventy-two patients were randomized into a treatment group and a control group by using the random number table, 36 cases each. The treatment group received acupuncture plus bloodletting therapy, while the control group was intervened by oral administration of estazolam. One-week treatment was taken as a course, for 3 successive courses in total. A follow-up study was conducted 3 months later. The Pittsburgh sleep quality index （PSQI） was evaluated before and after the intervention, as well as in the follow-up. The clinical efficacies were also compared. Results： The total effective rate was 88.9% in the treatment group versus 83.3% in the control group, and the between-group difference was statistically significant （P〈0.O5）. After the intervention, the PSO.I scores dropped significantly in both groups （both P〈0.05）; the between-group difference in PSQI score was statistically significant （P〈O.05）. The follow-up study showed that the between-group difference in the global score of PSO, I was statistically significant （P〈0.05）. Conclusion： Acupuncture plus bloodletting therapy can produce a more significant efficacy than oral administration of estazolam in treating insomnia in people with blood stasis constitution.
