[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []V...[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.展开更多
Aim To develop a reverse phase HPLC method for the determination of aloperine, an alkaloid that is newly extracted from Sophora alopecuraides and has shown wide pharmacological effects including antibacterial and...Aim To develop a reverse phase HPLC method for the determination of aloperine, an alkaloid that is newly extracted from Sophora alopecuraides and has shown wide pharmacological effects including antibacterial and antiinflammatory actions. Methods The samples were analyzed on a ODS column with methanol water triethylamine (3∶97∶0 1 V/V) as a mobile phase. The flow rate was 1 0 mL·min -1 , and UV detection wavelength 205 nm. Results Linear regression equation was A=1 6920C+1 7455 (r 2=0 9999, n =5) in concentratins ranging from 20 to 120 μg·mL -1 . The recoveries were 101 2±1 46 % at 80 μg·mL -1 , 100 5±0 75% at 100 μg·mL -1 , and 100 7±1 10% at 120 μg·mL -1 , respectively, and the precisions of aloperine within or between run were from 0 80% to 1 98% ( n =5). The relative contents of aloperine in three lots of tablets were 101 59±1 38%, 98 46±0 23%, and 99 41±1 09% ( n =3). Conclusion The newly developed reverse phase HPLC method is simple and useful for daily assay of aloperine tablets and can overcome the interference from excipient and other alkaloids in titration and UV detection.展开更多
Objective The treatment planning system currently represents one of the basics of radiation therapy,because it is the only method to estimate patient dose delivery fast forward and accurately represent estimated tumor...Objective The treatment planning system currently represents one of the basics of radiation therapy,because it is the only method to estimate patient dose delivery fast forward and accurately represent estimated tumor location of the tumor with the possibility of estimating densities in the tissue surrounding the tumor to overcome dose calculation defects but radial estimated the patient.Despite the flaws associated with the systems and calculates the dose of your programs in all programs currently existing in the world.Than necessary,to the existence of a review of the accuracy of accounts and how to confirm the radiation dose to the patient programs.Methods A total of 35 cancer patients were considered for this study,with 245 field measurements made with low-and high-energy diode detectors for brain and prostate cases.The treatments for all patients were planned using Eclipse Treatment Planning System version 13.6.Results Of the 105 field measurements made for the prostate cancer patients,16 included discrepancies outside the ±5% action level.Of the 145 measurements taken of the brain cases,there were four outside the ±5% action level.The results indicated a higher degree of accuracy.The study revealed that,for the prostate measurements,the higher discrepancy in the doses for the particular fields(exceeding the action level) may have been due to the isocenter being very close to the jaws and multi-leaf collimator of the linear accelerator machine.As a result,scatter from the jaws and the multi-leaf collimator could have contributed to the high dose delivered to the diode;hence,a probable higher discrepancy of the dose in more brain cases due highest quality of VMAT technique and fixation system.Conclusion A greater percentage of the observed discrepancies were well within the set tolerance level.However,it is recommended that the positioning of the diode on the patient's skin and the angular sensitivity of the diodes be reconsidered.It is also recommended that a more accurate calculation of expected diode values be performed,especially for fields that pass through the table.These efforts would achieve action levels of ±5%.展开更多
基金Supported by National Natural Science Foundation of China(31072155)Natural Science Foundation of Jiangsu Province(BK2010068)+1 种基金Fund for Independent Innovation of Agricultural Science in Jiangsu Province[CX(11)2060]Special Fund for Agroscientific Research in the Public Interest(201303041)~~
文摘[Objective] This study aimed to establish a method for quantitative detection of mRNA transcriptional level of SS2 adhesive related-factors of Streptococcus suis serotype 2 （SS2） by fluorescent quantitative PCR. []Vlethod] The gene fragments en- coding SS2 adhesive related-factors MRP, FBPS and CPS2J and a housekeeping gene aroA were amplified by reverse transcription PCR from the total RNA of SS2, cloned, and sequenced. The recombinant plasmids containing the target genes were constructed, and used as templates in Real-time PCR. [Result] Dynamic curves, stan- dard curves and melting curves of the adhesive related-factors and aroA were ob- tained by the optimized Real-time PCR system. The standard curves showed a good linear relationship between template copy number and circulation number, and the correlation coefficients （FF） of the standard curves were over 0.995. Also, these as- says were highly specific a^d there was single specific melting peak for every gene. Moreover, the assays were highly sensitive and had a detection limit of 1.0×102 copies in 1 μl of initial templates. Finally, it was highly repeatable and had a coeffi- cient of variation less than 2% for intra-assay. [Conclusion] This study will provide a way to reveal the adhesion mechanism of SS2 to different host cells at molecular level.
文摘Aim To develop a reverse phase HPLC method for the determination of aloperine, an alkaloid that is newly extracted from Sophora alopecuraides and has shown wide pharmacological effects including antibacterial and antiinflammatory actions. Methods The samples were analyzed on a ODS column with methanol water triethylamine (3∶97∶0 1 V/V) as a mobile phase. The flow rate was 1 0 mL·min -1 , and UV detection wavelength 205 nm. Results Linear regression equation was A=1 6920C+1 7455 (r 2=0 9999, n =5) in concentratins ranging from 20 to 120 μg·mL -1 . The recoveries were 101 2±1 46 % at 80 μg·mL -1 , 100 5±0 75% at 100 μg·mL -1 , and 100 7±1 10% at 120 μg·mL -1 , respectively, and the precisions of aloperine within or between run were from 0 80% to 1 98% ( n =5). The relative contents of aloperine in three lots of tablets were 101 59±1 38%, 98 46±0 23%, and 99 41±1 09% ( n =3). Conclusion The newly developed reverse phase HPLC method is simple and useful for daily assay of aloperine tablets and can overcome the interference from excipient and other alkaloids in titration and UV detection.
文摘Objective The treatment planning system currently represents one of the basics of radiation therapy,because it is the only method to estimate patient dose delivery fast forward and accurately represent estimated tumor location of the tumor with the possibility of estimating densities in the tissue surrounding the tumor to overcome dose calculation defects but radial estimated the patient.Despite the flaws associated with the systems and calculates the dose of your programs in all programs currently existing in the world.Than necessary,to the existence of a review of the accuracy of accounts and how to confirm the radiation dose to the patient programs.Methods A total of 35 cancer patients were considered for this study,with 245 field measurements made with low-and high-energy diode detectors for brain and prostate cases.The treatments for all patients were planned using Eclipse Treatment Planning System version 13.6.Results Of the 105 field measurements made for the prostate cancer patients,16 included discrepancies outside the ±5% action level.Of the 145 measurements taken of the brain cases,there were four outside the ±5% action level.The results indicated a higher degree of accuracy.The study revealed that,for the prostate measurements,the higher discrepancy in the doses for the particular fields(exceeding the action level) may have been due to the isocenter being very close to the jaws and multi-leaf collimator of the linear accelerator machine.As a result,scatter from the jaws and the multi-leaf collimator could have contributed to the high dose delivered to the diode;hence,a probable higher discrepancy of the dose in more brain cases due highest quality of VMAT technique and fixation system.Conclusion A greater percentage of the observed discrepancies were well within the set tolerance level.However,it is recommended that the positioning of the diode on the patient's skin and the angular sensitivity of the diodes be reconsidered.It is also recommended that a more accurate calculation of expected diode values be performed,especially for fields that pass through the table.These efforts would achieve action levels of ±5%.
