Optimal precursor of the transition from Kuroshio large meander to straight path

We used the conditional nonlinear optimal perturbation(CNOP) method to explore the optimal precursor of the transition from Kuroshio large meander(LM) to straight path within a barotropic inflowoutflow model,and found that large amplitudes of the optimal precursor are mainly located in the east of Kyushu,which implies that perturbations in the region are important for the transition from LM to straight path.Furthermore,we investigated the transition processes caused by the optimal precursor,and found that these processes could be divided into three stages.In the first stage,a cyclonic eddy is advected to the formation region of the Kuroshio large meander,which enhances the LM path and causes a cyclonic eddy to shed from the Kuroshio mainstream.This process causes the LM path to change into a small meander path.Subsequently,the small meander is maintained for a period because the vorticity advection is balanced by the beta effect in the second stage.In the third stage,the small meander weakens and the straight path ultimately forms.The positive vorticity advecting downstream is responsible for this process.The exploration of the optimal precursor will conduce to improve the prediction of the transition processes from LM path to straight path,and its spatial structure can be used to guide Kuroshio targeted observation studies.

高产表面活性素的重组枯草芽孢杆菌构建及培养优化

表面活性素是一种新型生物表面活性剂,因其具有良好的表面活性、可生物降解及抗菌活性,在石油开采、医药、农业和食品化妆品等领域具有广阔的应用前景。高产表面活性素菌株的获得和发酵过程优化是其商业化生产的关键。文中考察了脂肪酸合成途径对表面活性素合成的影响,强化脂肪酸生物合成关键基因以及该途径全部基因分别构建了高产表面活性素枯草芽孢杆菌Bacillus subtilis THBS-2和THBS-8,并对发酵过程中氨基酸种类及添加量、诱导剂异丙基-β-D-硫代半乳糖苷(IPTG)添加时间和添加量等条件对产物合成的影响进行考察,获得优化的两阶段前体添加方案:发酵3 h,加入IPTG和L-亮氨酸,使其终浓度分别为1.25 mmol/L、5 g/L;发酵24 h,添加L-亮氨酸(终浓度5 g/L)和浓缩培养基5 mL。优化条件下,枯草芽孢杆菌THBS-2摇瓶发酵48 h,表面活性素产量高达24 g/L;30 L发酵罐中发酵68 h,产物产量最高达到34 g/L。研究结果为表面活性素的工业化生产及应用奠定基础。