目的建立非去势SD大鼠良性前列腺增生(BPH)模型,明确皮下注射丙酸睾酮(TP)具有诱导大鼠前列腺增生的作用,考察合适的诱导剂量,评估模型的维持时间及其对肾功能、体质量和各脏器指数的影响。方法将TP诱导组的大鼠前列腺指数和前列腺组织...目的建立非去势SD大鼠良性前列腺增生(BPH)模型,明确皮下注射丙酸睾酮(TP)具有诱导大鼠前列腺增生的作用,考察合适的诱导剂量,评估模型的维持时间及其对肾功能、体质量和各脏器指数的影响。方法将TP诱导组的大鼠前列腺指数和前列腺组织病理学切片与对照组进行比较。对比对照组和TP高、中、低3个剂量组血清双氢睾酮(DHT)、胰岛素样生长因子-1(IGF-1)、表皮生长因子(EGF)和BCL-2相关X蛋白(Bax)表达水平,并记录肾功能指标尿素氮(UREA)、肌酐(CREA)和血糖(GLU)的变化情况。以造模4周完成当天(实验第4周)和造模完成后4周(实验第8周)为2个时间点,对模型组与对照组大鼠前列腺指数及DHT表达水平进行比较,确定维持时间;并对各脏器指数及每周的体质量变化进行检测。结果与对照组相比,TP高剂量组组织病理形态和前列腺指数显示明显增生,DHT、IGF-1和EGF的表达水平显著升高,Bax表达水平显著降低。TP高剂量组大鼠CREA和GLU的表达水平与对照组比较差异有显著统计学意义。第4周TP高剂量组大鼠DHT表达水平、前列腺指数、肾脏指数和睾丸指数均显著高于对照组,胸腺和肝指数显著低于对照组;第8周时,仅前列腺和肾脏指数显著高于对照组,其他脏器及DHT表达水平均恢复正常。结论采用皮下注射丙酸睾酮50 mg·kg^(-1),2 d 1次,连续4周可成功诱导非去势SD大鼠BPH模型,该模型维持时间少于28 d,可引起大鼠体质量及多项脏器指数异常,使得肾指数升高并对肾功能产生不利影响。展开更多
OBJECTIVE: To investigated the effect and mechanism of Fangjihuangqi Tang (FHT) on lower urinary tract dysfunction induced by benign prostatic hyperplasia (BPH) in rats. METHODS: Male rats were randomly divided into s...OBJECTIVE: To investigated the effect and mechanism of Fangjihuangqi Tang (FHT) on lower urinary tract dysfunction induced by benign prostatic hyperplasia (BPH) in rats. METHODS: Male rats were randomly divided into seven groups: normal, model, finasteride (0.5 mg/ kg), terazosin (0.5 mg/kg), and FHT (10, 5, 2.5 g/kg). Rats were administered testosterone (0.5 mg sc) for 6 weeks after orchiectomy, excluding the normal group. All rats were intragastrically administered assigned drugs for 4 weeks from the third week. Urodynamics were assessed in rats under anesthesia. Serum dihydrotestosterone (DHT) and prostatic acid phosphatase (PAP) were measured. The prostate index (PI), bladder index (BI), and pathological detection were evaluated. RESULTS: In the model group, the PI, BI, serum DHT, serum PAP, threshold pressure (TP), micturition pressure (MP), and residual urine volume (RV)were significantly higher. Moreover, inter-micturition duration (IMD) was significantly lower and the prostatic and bladder showed obvious pathological changes. The IMD was significantly higher, while BI, TP, MP, and RV were significantly lower and bladder pathological changes were alleviated in the FHT (10, 5 g/kg), finasteride, and terazosin groups. The PI, DHT, and PAP were significantly lower in the finasteride group, but they did not change significantly in the FHT (10, 5, 2.5 g/kg) and terazosin groups. CONCLUSION: FHT could relieve symptoms of lower urinary tract dysfunction in BPH rats but with no apparent effect on reducing the volume of the enlarged prostate itself.展开更多
文摘目的建立非去势SD大鼠良性前列腺增生(BPH)模型,明确皮下注射丙酸睾酮(TP)具有诱导大鼠前列腺增生的作用,考察合适的诱导剂量,评估模型的维持时间及其对肾功能、体质量和各脏器指数的影响。方法将TP诱导组的大鼠前列腺指数和前列腺组织病理学切片与对照组进行比较。对比对照组和TP高、中、低3个剂量组血清双氢睾酮(DHT)、胰岛素样生长因子-1(IGF-1)、表皮生长因子(EGF)和BCL-2相关X蛋白(Bax)表达水平,并记录肾功能指标尿素氮(UREA)、肌酐(CREA)和血糖(GLU)的变化情况。以造模4周完成当天(实验第4周)和造模完成后4周(实验第8周)为2个时间点,对模型组与对照组大鼠前列腺指数及DHT表达水平进行比较,确定维持时间;并对各脏器指数及每周的体质量变化进行检测。结果与对照组相比,TP高剂量组组织病理形态和前列腺指数显示明显增生,DHT、IGF-1和EGF的表达水平显著升高,Bax表达水平显著降低。TP高剂量组大鼠CREA和GLU的表达水平与对照组比较差异有显著统计学意义。第4周TP高剂量组大鼠DHT表达水平、前列腺指数、肾脏指数和睾丸指数均显著高于对照组,胸腺和肝指数显著低于对照组;第8周时,仅前列腺和肾脏指数显著高于对照组,其他脏器及DHT表达水平均恢复正常。结论采用皮下注射丙酸睾酮50 mg·kg^(-1),2 d 1次,连续4周可成功诱导非去势SD大鼠BPH模型,该模型维持时间少于28 d,可引起大鼠体质量及多项脏器指数异常,使得肾指数升高并对肾功能产生不利影响。
基金Supported by Natural Science Foundation of the Education Department of Anhui Province,China(No.KJ2010A208)
文摘OBJECTIVE: To investigated the effect and mechanism of Fangjihuangqi Tang (FHT) on lower urinary tract dysfunction induced by benign prostatic hyperplasia (BPH) in rats. METHODS: Male rats were randomly divided into seven groups: normal, model, finasteride (0.5 mg/ kg), terazosin (0.5 mg/kg), and FHT (10, 5, 2.5 g/kg). Rats were administered testosterone (0.5 mg sc) for 6 weeks after orchiectomy, excluding the normal group. All rats were intragastrically administered assigned drugs for 4 weeks from the third week. Urodynamics were assessed in rats under anesthesia. Serum dihydrotestosterone (DHT) and prostatic acid phosphatase (PAP) were measured. The prostate index (PI), bladder index (BI), and pathological detection were evaluated. RESULTS: In the model group, the PI, BI, serum DHT, serum PAP, threshold pressure (TP), micturition pressure (MP), and residual urine volume (RV)were significantly higher. Moreover, inter-micturition duration (IMD) was significantly lower and the prostatic and bladder showed obvious pathological changes. The IMD was significantly higher, while BI, TP, MP, and RV were significantly lower and bladder pathological changes were alleviated in the FHT (10, 5 g/kg), finasteride, and terazosin groups. The PI, DHT, and PAP were significantly lower in the finasteride group, but they did not change significantly in the FHT (10, 5, 2.5 g/kg) and terazosin groups. CONCLUSION: FHT could relieve symptoms of lower urinary tract dysfunction in BPH rats but with no apparent effect on reducing the volume of the enlarged prostate itself.
