前列腺组织中睾酮含量状态与前列腺生理和增生的关系

目的 检测前列腺组织中总睾酮、结合睾酮和游离睾酮的含量 ,并分析其与前列腺生理和组织增生的关系。方法 14例相对年轻正常前列腺组织、2 2例良性前列腺增生 (BPH)组织和 11例相应年龄段前列腺对照组织 ,制备组织胞浆和胞核提取液 ,乙醚萃取分离游离和结合睾酮 ,采用12 5I标记睾酮放免试剂盒测定睾酮的含量。结果 前列腺组织中睾酮存在结合和游离 2种状态 ,结合态为多 ,约占 2 / 3;3组比较 ,总睾酮、游离和结合睾酮含量无显著差异。结论 前列腺组织中睾酮存在游离和结合二种状态 ,共同维持局部高水平的总睾酮含量 ,并且随年龄增长而保持稳定 。

电生理刺激联合中药坐浴治疗慢性前列腺炎的临床效果观察

目的:探讨电生理刺激与中药坐浴联合治疗慢性前列腺炎的临床效果观察。方法:研究对象为2018年8月1日-2019年8月1日笔者所在医院住院的86例慢性前列腺炎患者。根据治疗方法不同进行分组,将39例单纯行前列腺电生理刺激治疗的患者设为对照组,将47例采用前列腺电生理刺激与中药坐浴联合治疗的患者设为治疗组。两组均治疗2周。比较两组治疗前后前列腺液中卵磷脂小体计数、白细胞计数、NIH-CPSI评分。结果:治疗前两组患者前列腺液中卵磷脂小体计数、白细胞计数及NIH-CPSI评分比较,差异均无统计学意义(P>0.05);治疗2周后,治疗组卵磷脂小体计数、白细胞计数及NIH-CPSI评分均优于对照组,差异有统计学意义(P<0.05)。结论:与单纯行前列腺电生理刺激治疗慢性前列腺炎相比较,联合中药坐浴共同治疗效果更好。

光活化的金丝桃素对人前列腺癌细胞PC3M的生长抑制作用

目的 :探讨光活化的金丝桃素对人前列腺癌细胞 PC3M的抑癌作用。方法 :用生长抑制试验和流式细胞技术观察给予金丝桃素后 PC3M细胞的增殖、细胞周期和凋亡的变化。结果 :不同浓度的金丝桃素在体外可显著抑制 PC3M细胞增殖 (P<0 .0 5 ) ,药物浓度为 0 .5、 1 .0和 2 .0 m g· L- 1 时 ,抑制率分别为 2 1 %、 5 0 %和 91 % ;细胞周期出现 S期阻滞 ,由 S期进入 M2 期的比例为零 ,并诱导 PC3M细胞凋亡。结论

阿仑膦酸钠对前列腺癌患者胰岛素样生长因子1、胰岛素样生长因子结合蛋白3测定的意义

目的:探讨阿仑膦酸钠对前列腺癌患者胰岛素样生长因子1、胰岛素样生长因子结合蛋白3的影响。方法:选取前列腺癌患者76例,按照随机数字表法分为对照组和试验组各38例。对照组给予抗雄激素1周后予以钙元素治疗,试验组在对照组基础上予以阿仑膦酸钠治疗,两组共治疗12个月为一疗程。比较治疗前、治疗6个月后、治疗12个月骨密度、血清P1NP、β-CTX、IGF-1、IGFBP-3表达水平及不良反应状况。结果:与治疗前比较,对照组治疗12个月后腰椎、股骨颈、全髋部骨密度降低,血清P1NP、β-CTX表达升高,治疗6个月、12个月后血清IGF-1表达降低、血清IGFBP-3表达升高,试验组治疗12个月后腰椎、股骨颈、全髋部骨密度升高,血清P1NP、β-CTX表达降低,治疗6个月、12个月后血清IGF-1表达降低、血清IGFBP-3表达升高(P<0.05);与对照组比较,试验组治疗12个月后腰椎、股骨颈、全髋部骨密度较高,治疗6个月、12个月后血清P1NP、β-CTX表达较低,治疗6个月、12个月后血清IGF-1表达较低、血清IGFBP-3表达升高(P<0.05)。结论:阿仑膦酸钠有利于预防前列腺癌去势患者骨质流失现象,降低骨转换率,改善IGF系统,从而提高前列腺癌患者预后。

柯萨奇腺病毒受体在前列腺癌细胞中的表达及分析

目的:讨论柯萨奇腺病毒受体(CAR)在前列腺癌恶性进展中的作用。方法:Western-Blot鉴定CAR在LNCaP、C4、C4-2、C4-2B、DU145与PC-3等6种前列腺癌细胞株中的表达情况。结果:CAR在低恶性的LNCaP细胞中表达最高,在高度恶性、有较强远处转移能力的C4、C4-2、C4-2B、DU145与PC-3细胞中的表达呈依次下降趋势,在恶性程度最高的PC-3细胞中最低。结论:CAR在恶性程度不一的前列腺癌细胞中存在表达差异,可能在前列腺癌的恶性进展过程中发挥了重要作用。

手术治疗的良性前列腺增生患者PSA水平增高的相关因素分析

目的:了解手术治疗的良性前列腺增生患者PSA水平增高的相关因素。材料和方法:选取手术治疗的良性前列腺增生患者共369例,取患者的PSA、前列腺体积、残余尿、最大尿流率及国际前列腺症状评分(IPSS)共5项指标进行相关性分析。结果:手术治疗的良性前列腺增生患者血清PSA水平与前列腺体积和年龄呈显著正相关。患者的IPSS与血清PSA水平具有一定的正相关性。患者的PSA与残余尿量和最大尿流率均无明显相关性。结论:在影响血清PSA的相关因素中,年龄、前列腺体积对血清PSA的水平起决定性作用;IPSS可能在一定程度上和PSA的水平相关。

TURP大量灌洗液对老年患者血液流变学影响的研究

目的：探讨经尿道前列腺电切手术（TURP）中,灌洗液致血液稀释对循环功能及血液流变学的影响.方法：选择ASAⅠ～Ⅱ级32例前列腺增生（BPH）患者,分别于术前（T0）、灌洗12000ml（T1）时、灌洗24000ml（T2）时,采静脉血测血液流变学参数,同时采用多功能监护仪监测平均动脉压（MAP）、心律（HR）、心电图（ECG）、氧饱和度（SpO2）.结果：患者术中循环稳定,在T1、T2时段MAP、SpO2略有升高,心率略有下降,但无显著性差异（P＞0.05）;T1、T2全血粘度、红细胞聚集指数、Hb、Hct较均T0显著降低（P＜0.05）,T1、T2红细胞变形指数较T0升高但无显著性差异（P＞0.05）;T1与T2各项观察指标无显著性差异（P＞0.05）.结论：经尿道前列腺电切术灌洗液灌注,可致一定程度血液稀释,降低血液黏滞性,利于微循环灌注,在一定灌注量范围内对血流动力学影响不大.

Network pharmacology research and experimental verification of Huangqi(Astragalus Radix)and Jinyingzi(Rosae Laevigatae Fructus)in treating benign prostatic hyperplasia

Objective This study aimed to analyze the mechanism of action of Huangqi(Astragalus Radix,HQ)-Jinyingzi(Rosae Laevigatae Fructus,JYZ)in the treatment of benign prostatic hyperplasia(BPH)based on network pharmacology and to verify the prediction through animal experimentation.Methods Based on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine(BATMAN-TCM)databases,and literature,the active components and related target genes of HQ and JYZ were screened.The BPH target genes were screened based on the DisGeNET and GeneGards databases,and Excel was used to merge and remove duplicates.The Perl language was used to obtain drug-BPH target genes by intersecting shared target genes.A drug-component-target gene network diagram was constructed using Cytoscape software.The drug-BPH intersection target genes were inputted into the STRING database,and the key target genes were selected according to the degree algorithm.The output formed the basis for Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses to determine the potential mechanism of HQ and JYZ in BPH treatment.High,medium,and low doses of HQ and JYZ extract were used to intervene in BPH rats,and then the prostate volume,wet weight,and prostate index of the BPH rats were determined.Changes in prostate histopathology and microvessel density(MVD)were evaluated using immunohistochemistry,and the optimal HQ and JYZ extract dose was confirmed.Finally,the optimal dose was used to intervene in a BPH rat model,and AKT1 and VEGF expressions were examined by immunohistochemistry.Results Based on network pharmacology,33 active components and 772 target genes were identified from HQ and JYZ,along with 817 BPH target genes and 112 drug-BPH common target genes.Among them were 10 key target genes,including AKT1,JUN,MAPK1,IL-6,TNF,ESR1,and VEGFA.KEGG enrichment analysis revealed 135 signaling pathways,including PI3K/AKT,IL-17,TNF,p53,MAPK,VEGF,JAK-STAT,and NF-κB pathways.The animal experiment showed that HQ and JYZ significantly improved prostate volume,wet weight,prostate index,and prostate histopathology of BPH rats,reducing MVD.In addition,HQ and JYZ inhibited the expression of AKT1 and VEGF in the prostate tissue of rats,promoted epithelial cell apoptosis,and inhibited angiogenesis,consistent with the prediction.Conclusion The combination of HQ and JYZ is effective for BPH therapy through multi-compound and multi-target collaboration.Its possible mechanism in treating BPH includes regulation of AKT1,VEGF protein,PI3K/Akt,and VEGF signaling pathways related to apoptosis,angiogenesis,and inflammation,with potential for clinical use and research.

Therapeutic Effect of Massotherapy for Prospermia

Clinical DataAll the 60 cases of prospermia treated in thisseries were outpatients of our hospital.Their ageranged from 24 to 46 years,averaging 36.4 years.The prospermia caused by chronic prostatitis wasfound in 26 cases,urethritis in 8 cases,andcolliculitis in 2 cases,and the rest were functionalprospermia.The patients were divided into twogroups according to the patient's condition,age,andother factors.There were 30 cases in themassotherapy group,including 13 cases