Objective To investigate the effects of prostaglandin E1 (PGE1) on the progression of aristolochic acid nephropathy (AAN). Methods Twenty-four patients diagnosed as AAN with serum creatinine (Scr) between 1.5 mg/dL an...Objective To investigate the effects of prostaglandin E1 (PGE1) on the progression of aristolochic acid nephropathy (AAN). Methods Twenty-four patients diagnosed as AAN with serum creatinine (Scr) between 1.5 mg/dL and 4 mg/dL during September 2001 to August 2003 were randomly divided into 2 groups. All patients had ingested long dan xie gan wan con-taining aristolochic acid (0.219 mg/g) for at least 3 months. Twelve patients were injected with Alprostadil (10 μg/d for 10 days in one month, summing up to 6 months). Except for PGE1, the other therapy was same in both groups. Renal function was assessed using reciprocal serum creatinine levels (1/Scr). Results The level of Scr and serum hemoglobin (Hgb) was similar in both groups prior to therapy. During follow-up, 1/Scr levels in PGE1 group were significantly higher than control group (P < 0.01), and Hgb levels in PGE1 group were sig-nificantly increased compared with control (P < 0.05).Conclusion PGE1 can slow the progression of renal failure and increase Hgb level of AAN patient.展开更多
AIM： To observe the effect of Fructus Psoraleae on motility of isolated gallbladder muscle strips of guinea pigs and its mechanism. METHODS： Guinea pigs were hit to lose consciousness and the whole gallbladder was r...AIM： To observe the effect of Fructus Psoraleae on motility of isolated gallbladder muscle strips of guinea pigs and its mechanism. METHODS： Guinea pigs were hit to lose consciousness and the whole gallbladder was removed quickly. Two or three smooth muscle strips （8 mm×3mm） were cut along a longitudinal direction. The mucosa was gently removed. Every longitudinal muscle strip was suspended in a tissue chamber which was continuously perfused with 5 mL Krebs solution （37℃）, pH 7.4, and aerated with 950 mL/L 02 and 50 mL/L CO2. The isometric response was recorded with an ink-writing recorder. After 2 h equilibration under i g-load, 50 μL Fructus Psoraleae （10, 20, 70, 200, 700, 1000 g/L） was added cumulatively into the tissue chamber in turn every 2 rain to observe their effects on gallbladder muscle strips （cumulating final concentration of Fructus Psoraleae was 0.1, 0.3, 1.0, 3.0, 10.0, 20.0 g/L）. The antagonists, including 4-DAMP, benzhydramine, hexamethonium, phentolamine, verapamil and idomethine were given 2 min before Fructus Psoraleae respectively to investigate the mechanisms involved. RESULTS： Fructus Psoraleae dose-dependently increased the resting tension （r=0.992, P〈0.001）, decreased the mean contractile amplitude （r=0.970, P〈0.001） and meanwhile increased the contractile frequency of the gallbladder muscle strip in vitro （r=0.965, P〈0.001）. The exciting action of Fructus Psoraleae on the resting tension could be partially blocked by 4-DAMP （the resting tension decreased from 1.37 ± 0.41 to 0.70 ± 0.35, P〈0.001）, benzhydramine （from 1.37 ±0.41 to 0.45±0.38, P〈0.001）, hexamethonium （from 1.37 ± 0.41 to 0.94 ± 0.23, P〈0.05）, phentolamine （ from 1.37±0.41 to 0.89±0.22, P〈0.01） and verapamil （from 1.37±0.41 to 0.94±0.26, P〈0.05）. But the above antagonists had no significant effect on the action of Fructus Psoraleae-induced mean contractile amplitude （P〉0.05）. Moreover, the increase of the contractile frequency due to Fructus Psoraleae was inhibited by 4-DAMP （decreased from 8.3 ± 1.2 to 6.8 ± 0.5, P 〈 0.01） and hexamethonium （from 8.3 ±1.2 to 7.0 ± 0.9, P 〈 0.05）. Idomethine had no significant effect on the Fructus Psoraleae- induced responses （P〉 0.05）. CONCLUSION： Fructus Psoraleae enhances the motility of isolated gallbladder muscle strips from guinea pigs, in a dose-dependent manner. The effect of Fructus Psoraleae is partly related to M3, N receptor, α receptor, H1 receptor, Ca^2＋ channel, but not related to prostaglandin.展开更多
Objective: To evaluate cyclooxygenase-2 (COX-2) expression induced by insulin in cultured rat vascular muscle smooth cells (VSMCs). Methods: The rat VSMCs were isolated and cultured in 60 mm plates, pre-treated with i...Objective: To evaluate cyclooxygenase-2 (COX-2) expression induced by insulin in cultured rat vascular muscle smooth cells (VSMCs). Methods: The rat VSMCs were isolated and cultured in 60 mm plates, pre-treated with insulin 10, 100, 500, 1 000 mU/L for 6 h, and 100 mU/L for 3, 6, 12, and 24 h, respectively. RT-PCR was performed to measure COX-2 mRNA induction, and Western-blot was used to measure protein expression. In regard to the COX-2 enzyme activity, after stimulation for a specified duration, the culture medium was collected; Prostaglandin E2 (PGE2) released by VSMCs was measured with an EIA kit. Results: Three hours after insulin (100 mU/L) stimulation, the induction of COX-2 mRNA was detectable and increased with the time and dosage of insulin. Insulin induced COX-2 protein expression occurred in a time- and dose-dependent manner. In rat VSMCs, COX-2 induced by insulin resulted in PGE2 production, which was increased with insulin action. Conclusion: Insulin could induce COX-2 expression and PGE2 production in VSMCs in time and dose-dependent manners.展开更多
Objective:Robot-assisted radical prostatectomy(RARP)requires pneumoperitoneum(Pnp)and a steep head-down position that may disturb respiratory system compliance(Crs)during surgery.Our aim was to compare the effects of ...Objective:Robot-assisted radical prostatectomy(RARP)requires pneumoperitoneum(Pnp)and a steep head-down position that may disturb respiratory system compliance(Crs)during surgery.Our aim was to compare the effects of different degrees of neuromuscular block(NMB)on Crs with the same Pnp pressure during RARP.Methods:One hundred patients who underwent RARP were enrolled and randomly allocated to a deep or moderate NMB group with 50 patients in each group.Rocuronium was administered to both groups:in the moderate NMB group to maintain 1–2 responses to train-of-four(TOF)stimulation;and in the deep NMB group to maintain no response to TOF stimulation and 1–2 responses in the post-tetanic count.Pnp pressure in both groups was 10 mm Hg(1 mm Hg=133.3 Pa).Peak inspiratory pressure(Ppeak),mean pressure(Pmean),Crs,and airway resistance(Raw)were recorded after anesthesia induction and at 0,30,60,and 90 min of Pnp and post-Pnp.Surgical space conditions were evaluated after the procedure on a 4-point scale.Results:Immediately after the Pnp,Ppeak,Pmean,and Raw significantly increased,while Crs decreased and persisted during Pnp in both groups.The results did not significantly differ between the two groups at any of the time points.There was no difference in surgical space conditions between groups.Body movements occurred in 14 cases in the moderate NMB group and in one case in the deep NMB group,and all occurred during obturator lymphadenectomy.A significant difference between the two groups was observed.Conclusions:Under the same Pnp pressure in RARP,deep and moderate NMBs resulted in similar changes in Crs,and in other respiratory mechanics and surgical space conditions.However,deep NMB significantly reduced body movements during surgery.展开更多
文摘Objective To investigate the effects of prostaglandin E1 (PGE1) on the progression of aristolochic acid nephropathy (AAN). Methods Twenty-four patients diagnosed as AAN with serum creatinine (Scr) between 1.5 mg/dL and 4 mg/dL during September 2001 to August 2003 were randomly divided into 2 groups. All patients had ingested long dan xie gan wan con-taining aristolochic acid (0.219 mg/g) for at least 3 months. Twelve patients were injected with Alprostadil (10 μg/d for 10 days in one month, summing up to 6 months). Except for PGE1, the other therapy was same in both groups. Renal function was assessed using reciprocal serum creatinine levels (1/Scr). Results The level of Scr and serum hemoglobin (Hgb) was similar in both groups prior to therapy. During follow-up, 1/Scr levels in PGE1 group were significantly higher than control group (P < 0.01), and Hgb levels in PGE1 group were sig-nificantly increased compared with control (P < 0.05).Conclusion PGE1 can slow the progression of renal failure and increase Hgb level of AAN patient.
基金Supported by Key Laboratory of Pre-clinical Study for New Drugs of Gansu Province and the Natural Science Foundation of Gansu Province, No.ZS021-A25-059-Y
文摘AIM： To observe the effect of Fructus Psoraleae on motility of isolated gallbladder muscle strips of guinea pigs and its mechanism. METHODS： Guinea pigs were hit to lose consciousness and the whole gallbladder was removed quickly. Two or three smooth muscle strips （8 mm×3mm） were cut along a longitudinal direction. The mucosa was gently removed. Every longitudinal muscle strip was suspended in a tissue chamber which was continuously perfused with 5 mL Krebs solution （37℃）, pH 7.4, and aerated with 950 mL/L 02 and 50 mL/L CO2. The isometric response was recorded with an ink-writing recorder. After 2 h equilibration under i g-load, 50 μL Fructus Psoraleae （10, 20, 70, 200, 700, 1000 g/L） was added cumulatively into the tissue chamber in turn every 2 rain to observe their effects on gallbladder muscle strips （cumulating final concentration of Fructus Psoraleae was 0.1, 0.3, 1.0, 3.0, 10.0, 20.0 g/L）. The antagonists, including 4-DAMP, benzhydramine, hexamethonium, phentolamine, verapamil and idomethine were given 2 min before Fructus Psoraleae respectively to investigate the mechanisms involved. RESULTS： Fructus Psoraleae dose-dependently increased the resting tension （r=0.992, P〈0.001）, decreased the mean contractile amplitude （r=0.970, P〈0.001） and meanwhile increased the contractile frequency of the gallbladder muscle strip in vitro （r=0.965, P〈0.001）. The exciting action of Fructus Psoraleae on the resting tension could be partially blocked by 4-DAMP （the resting tension decreased from 1.37 ± 0.41 to 0.70 ± 0.35, P〈0.001）, benzhydramine （from 1.37 ±0.41 to 0.45±0.38, P〈0.001）, hexamethonium （from 1.37 ± 0.41 to 0.94 ± 0.23, P〈0.05）, phentolamine （ from 1.37±0.41 to 0.89±0.22, P〈0.01） and verapamil （from 1.37±0.41 to 0.94±0.26, P〈0.05）. But the above antagonists had no significant effect on the action of Fructus Psoraleae-induced mean contractile amplitude （P〉0.05）. Moreover, the increase of the contractile frequency due to Fructus Psoraleae was inhibited by 4-DAMP （decreased from 8.3 ± 1.2 to 6.8 ± 0.5, P 〈 0.01） and hexamethonium （from 8.3 ±1.2 to 7.0 ± 0.9, P 〈 0.05）. Idomethine had no significant effect on the Fructus Psoraleae- induced responses （P〉 0.05）. CONCLUSION： Fructus Psoraleae enhances the motility of isolated gallbladder muscle strips from guinea pigs, in a dose-dependent manner. The effect of Fructus Psoraleae is partly related to M3, N receptor, α receptor, H1 receptor, Ca^2＋ channel, but not related to prostaglandin.
文摘Objective: To evaluate cyclooxygenase-2 (COX-2) expression induced by insulin in cultured rat vascular muscle smooth cells (VSMCs). Methods: The rat VSMCs were isolated and cultured in 60 mm plates, pre-treated with insulin 10, 100, 500, 1 000 mU/L for 6 h, and 100 mU/L for 3, 6, 12, and 24 h, respectively. RT-PCR was performed to measure COX-2 mRNA induction, and Western-blot was used to measure protein expression. In regard to the COX-2 enzyme activity, after stimulation for a specified duration, the culture medium was collected; Prostaglandin E2 (PGE2) released by VSMCs was measured with an EIA kit. Results: Three hours after insulin (100 mU/L) stimulation, the induction of COX-2 mRNA was detectable and increased with the time and dosage of insulin. Insulin induced COX-2 protein expression occurred in a time- and dose-dependent manner. In rat VSMCs, COX-2 induced by insulin resulted in PGE2 production, which was increased with insulin action. Conclusion: Insulin could induce COX-2 expression and PGE2 production in VSMCs in time and dose-dependent manners.
基金Project supported by the Zhejiang Province Public Welfare Technology Application Research Project(No.LGF20H010006),China。
文摘Objective:Robot-assisted radical prostatectomy(RARP)requires pneumoperitoneum(Pnp)and a steep head-down position that may disturb respiratory system compliance(Crs)during surgery.Our aim was to compare the effects of different degrees of neuromuscular block(NMB)on Crs with the same Pnp pressure during RARP.Methods:One hundred patients who underwent RARP were enrolled and randomly allocated to a deep or moderate NMB group with 50 patients in each group.Rocuronium was administered to both groups:in the moderate NMB group to maintain 1–2 responses to train-of-four(TOF)stimulation;and in the deep NMB group to maintain no response to TOF stimulation and 1–2 responses in the post-tetanic count.Pnp pressure in both groups was 10 mm Hg(1 mm Hg=133.3 Pa).Peak inspiratory pressure(Ppeak),mean pressure(Pmean),Crs,and airway resistance(Raw)were recorded after anesthesia induction and at 0,30,60,and 90 min of Pnp and post-Pnp.Surgical space conditions were evaluated after the procedure on a 4-point scale.Results:Immediately after the Pnp,Ppeak,Pmean,and Raw significantly increased,while Crs decreased and persisted during Pnp in both groups.The results did not significantly differ between the two groups at any of the time points.There was no difference in surgical space conditions between groups.Body movements occurred in 14 cases in the moderate NMB group and in one case in the deep NMB group,and all occurred during obturator lymphadenectomy.A significant difference between the two groups was observed.Conclusions:Under the same Pnp pressure in RARP,deep and moderate NMBs resulted in similar changes in Crs,and in other respiratory mechanics and surgical space conditions.However,deep NMB significantly reduced body movements during surgery.
