Transmission electron microscopy (TEM) observation of pollen grains of 18 species belongingto 18 respective genera of the tribe Peucedaneae Drude revealed distinct ultrastructural difference in thepollen exine, includ...Transmission electron microscopy (TEM) observation of pollen grains of 18 species belongingto 18 respective genera of the tribe Peucedaneae Drude revealed distinct ultrastructural difference in thepollen exine, including the thickness and features of rectum, columnar layer, foot layer and endexine. Thesystematic position of those 18 species are re-evaluated based on their ultrastructural characteristics ofpollen exine observed in this study. Seven genera related to Angelica L. in Angelicinae Drude have beendeduced to genus Angelica L. For example, Osterfcum grosseserratum (Maxim.) Kitagawa was changed toAngelica L. by some authors, but it differs from Angelica sinensis (Oliv.) Diels in having well developedtectum which is thicker than the columnar layer and foot layer. Its columnar layer is quite well-developedwith long and branched columellae. Besides its surface is tuberculated. Evidently, its development exceedsthat of Angelica sinensis. However, it is only a moderate evolutional species in its genus, and the Angelicasinensis accounts as the most advanced species in Angelica Diels, thus, placing genus Ostencum Hoffm.in Angelica L is not suitable, it may be more appropriate to keep its original position. In addition, Ferulaakitschkensis B. Fedtsch. ex K.-Pol. differs greatly from those of Talassia transiliensis (Herd.) Korov. andSchumannia turcomnnia Kuntze, because of its very well-developed columnar layer, being about four tofive times thicker than the total of tectum and foot layer, tuberculated tectum surface and complicatedstructure of columnellae. As another species Heracleum forrestJ'iWolff also differs from Semenovia rubtzovii(Schischk.) Monden. in having an even thickness of exine and well-developed columnar layer, it seemssuitable that they should also be treated as two independent genera.展开更多
文摘采用PCR直接测序法对伞形科（ Apiaceae）前胡族（ Trib. Peucedaneae）种类川明参（ Chuanminshen violaceum Sheh et Shan）、泰山前胡〔Peucedanum wawrae （ H. Wolff） Su〕和华中前胡（ P. medicum Dunn）以及美味芹族（ Trib. Smyrnieae）种类明党参（Changium smyrnioides H. Wolff）、宝兴棱子芹（Pleurospermum davidii Franch.）、丽江棱子芹（ P. foetens Franch.）和鸡冠棱子芹（ P. cristatum de Boiss.）的叶绿体基因组psbA-trnH片段进行了扩增测序,获得的序列已提交至GenBank,登录号为KF557756-KF557762。结合引自GenBank的前胡族阿魏属（ Ferula Linn.）1种、大瓣芹属（ Semenovia Regel et Herder）1种、当归属（ Angelica Linn.）2种和美味芹族的舟瓣芹属（ Sinolimprichtia H. Wolff）1种、羌活属（ Notopterygium de Boiss.）1种、瘤果芹属（ Trachydium Lindl.）1种以及针果芹族（ Trib. Scandicineae）刺果芹〔Turgenia latifolia （ Linn.） Hoffm.〕的psbA-trnH片段序列,对各种类的psbA-trnH片段信息进行分析；并以刺果芹为外类群构建了MP、ML和BI系统发育树。结果表明：川明参和明党参的psbA-trnH片段长度均为258 bp、GC含量均为23％,而其他种类的psbA-trnH片段长度为228~405 bp、GC含量为26％~35％；排序后psbA-trnH 序列总长度为553 bp（包括空位）,其中变异位点237个、信息位点178个。川明参与明党参间的相对遗传距离最小（仅为0.02）,而川明参与其他种类间的相对遗传距离为0.10~1.34,且总体上川明参与美味芹族种类的相对遗传距离较小,表明川明参与明党参及美味芹族种类的亲缘关系较近。在3类系统树上,川明参与明党参均聚在一起,并与美味芹族的属种聚为一大支,而远离由前胡族属种构成的另一大支。结合外部形态和果实解剖结构特征,建议将川明参属（ Chuanminshen Sheh et Shan ）从前胡族中分出并置于美味芹族中,与明党参属（ Changium H. Wolff）为姐妹类群。
文摘Transmission electron microscopy (TEM) observation of pollen grains of 18 species belongingto 18 respective genera of the tribe Peucedaneae Drude revealed distinct ultrastructural difference in thepollen exine, including the thickness and features of rectum, columnar layer, foot layer and endexine. Thesystematic position of those 18 species are re-evaluated based on their ultrastructural characteristics ofpollen exine observed in this study. Seven genera related to Angelica L. in Angelicinae Drude have beendeduced to genus Angelica L. For example, Osterfcum grosseserratum (Maxim.) Kitagawa was changed toAngelica L. by some authors, but it differs from Angelica sinensis (Oliv.) Diels in having well developedtectum which is thicker than the columnar layer and foot layer. Its columnar layer is quite well-developedwith long and branched columellae. Besides its surface is tuberculated. Evidently, its development exceedsthat of Angelica sinensis. However, it is only a moderate evolutional species in its genus, and the Angelicasinensis accounts as the most advanced species in Angelica Diels, thus, placing genus Ostencum Hoffm.in Angelica L is not suitable, it may be more appropriate to keep its original position. In addition, Ferulaakitschkensis B. Fedtsch. ex K.-Pol. differs greatly from those of Talassia transiliensis (Herd.) Korov. andSchumannia turcomnnia Kuntze, because of its very well-developed columnar layer, being about four tofive times thicker than the total of tectum and foot layer, tuberculated tectum surface and complicatedstructure of columnellae. As another species Heracleum forrestJ'iWolff also differs from Semenovia rubtzovii(Schischk.) Monden. in having an even thickness of exine and well-developed columnar layer, it seemssuitable that they should also be treated as two independent genera.
