鸡胚前脑细胞在各种支持物特别是人羊膜基膜上生长的定量研究

作者采用Ⅰ型胶原、人羊膜基膜(HABM)基质面、Fibronectin(FN)、多聚鸟氨酸(PO)、PO+FN、PO+Laminin(LN)、鼠尾胶原和 HABM 上皮面作为8天鸡胚前脑细胞生长的支持物,观察这些支持物对前脑细胞生存生长的影响。前脑细胞以每个孔不同的细胞密度在 PO、LN 和HABM 上皮面培养。发现每个孔4×10~5细胞密度细胞生长最好。用 MTT 做为线粒体脱氢酶底物的自动比色法,对前脑细胞在各种支持物上的生长进行了定量测定。结果表明,所测光密度值(OD)与细胞生存、生长成正比,且 HABM 上皮面是前脑细胞生长最好的支持物。各种支持物促进前脑细胞生长的顺序浓次是:HABM 上皮面>鼠尾胶原>PO+LN>PO+FN>PO>FN>HABM 基质面>Ⅰ型胶原。HABM 上皮面比基质面促进前脑细胞生长作用明显强(P<0.01);鼠尾胶原比Ⅰ型胶原强(P<0.05);PO+LN 比 PO 作用强(P<0.05)。这提示 LN 很可能是 HABM、鼠尾胶原和 PO+LN 中促进神经生长的主要活性因子。

Transplanted neuronal precursors migrate and differentiate in the devel-oping mouse brain

The subventricular zone (SVZ), lining the lateral ventricle in forebrain, retains a population of neuronal precursors with the ability of proliferation in adult mammals. To test the potential of neuronal precursors in adult mice, we transplanted adult SVZ cells labeled with fluorescent dye PKH26 into the lateral ventricle of the mouse brain in different development stages. The preliminary results indicated that the grafted cells were able to survive and migrate into multiple regions of the recipient brain, including SVZ, the third ventricle, thalamus, superior colliculus, inferior colliculus, cerebellum and olfactory bulb etc; and the amount of survival cells in different brain regions was correlated with the development stage of the recipient brain. Immunohistochemical studies showed that most of the grafted cells migrating into the specific target could express neuronal or astrocytic marker. Our results revealed that the neuronal precursors in adult SVZ still retained immortality and ability of proliferation, which is likely to be induced by some environmental factors.