目的建立SYBR Green Ⅰ荧光定量PCR技术测定HIV-1前病毒载量方法,并以其观察相关病毒进入抑制剂对HIV-1前病毒的作用。方法选择HIV-1的gag和env保守区域设计引物,建立SYBR Green I荧光定量PCR测定HIV-1前病毒载量的体系,并以其观察6μg/...目的建立SYBR Green Ⅰ荧光定量PCR技术测定HIV-1前病毒载量方法,并以其观察相关病毒进入抑制剂对HIV-1前病毒的作用。方法选择HIV-1的gag和env保守区域设计引物,建立SYBR Green I荧光定量PCR测定HIV-1前病毒载量的体系,并以其观察6μg/ml^6 ng/ml的重组病毒巨噬细胞炎症蛋白(rvMIP)、逆转录酶抑制剂(AZT)、膜融合抑制剂(T-20)、rvMIP+AZT、rvMIP+T-20对HIV-1前病毒载量的影响。结果所建立的荧光定量PCR体系的检测下限为101Copies,其标准曲线的相关系数为0.998 9,斜率为-4.925,截距为35.70;除6ng/ml的rvMIP、AZT、T-20外,三种药物处理后HIV-1前病毒载量均显著减小(P<0.05),并呈质量浓度依赖性;联合用药者前病毒载量显著低于单用rvMIP者(P<0.05),HIV-1前病毒载量rvMIP+T-20(rvMIP+AZT(rvMIP(T-20(AZT。结论本研究建立的SYBR Green Ⅰ荧光定量PCR检测方法具有灵敏、快速、准确及成本低廉等优点;与T-20或AZT联用可增强rvMIP对HIV-1前病毒的抑制作用。展开更多
Granite is usually composed of quartz,biotite,feldspar,and cracks,and the variation characteristics of these components could reflect the deformation and failure process of rock well.Taking granite as an example,the v...Granite is usually composed of quartz,biotite,feldspar,and cracks,and the variation characteristics of these components could reflect the deformation and failure process of rock well.Taking granite as an example,the video camera was used to record the deformation and failure process of rock.The distribution of meso-components in video images was then identified.The meso-components of rock failure precursors were also discussed.Moreover,a modified LSTM(long short-term memory method)based on SSA(sparrow search algorithm)was proposed to estimate the change of meso-components of rock failure precursor.It shows that the initiation and expansion of cracks are mainly caused by feldspar and quartz fracture,and when the quartz and feldspar exit the stress framework,rock failure occurs;the second large increase of crack area and the second large decrease of quartz or feldspar area may be used as a precursor of rock failure;the precursor time of rock failure based on meso-scopic components is about 4 s earlier than that observed by the naked eye;the modified LSTM network has the strongest estimation ability for quartz area change,followed by feldspar and biotite,and has the worst estimation ability for cracks;when using the modified LSTM network to predict the precursors of rock instability and failure,quartz and feldspar could be given priority.The results presented herein may provide reference in the investigation of rock failure mechanism.展开更多
The damage and failure law of rock mass with holes is of great significance to the stability control of roadways. This study investigates the mechanical properties and failure modes of porous rock masses under cyclic ...The damage and failure law of rock mass with holes is of great significance to the stability control of roadways. This study investigates the mechanical properties and failure modes of porous rock masses under cyclic loading, elucidates the acoustic emission (AE) characteristics and their spatial evolution, and establishes the interrelation among AE, stress, strain, time, and cumulative damage. The results reveal that the rock mass with holes and the intact rock mass show softening and hardening characteristics after cyclic loading. The plastic strain of the rock mass with holes is smaller than that of the intact rock mass, and the stress −strain curve shows hysteresis characteristics. Under uniaxial compression, the pore-bearing rock mass shows the characteristics of higher ringing count, AE energy, b-value peak, and more cumulative ringing count in the failure stage, while it shows lower characteristics under cyclic action. At the initial stage of loading, compared with the intact rock mass, the pore-containing rock mass shows the characteristics of a low b-value. The AE positioning and cumulative damage percentage are larger, and the AE positioning is denser around the hole. The specimen with holes is mainly shear failure, and the complete specimen is mainly tensile shear failure.展开更多
文摘目的建立SYBR Green Ⅰ荧光定量PCR技术测定HIV-1前病毒载量方法,并以其观察相关病毒进入抑制剂对HIV-1前病毒的作用。方法选择HIV-1的gag和env保守区域设计引物,建立SYBR Green I荧光定量PCR测定HIV-1前病毒载量的体系,并以其观察6μg/ml^6 ng/ml的重组病毒巨噬细胞炎症蛋白(rvMIP)、逆转录酶抑制剂(AZT)、膜融合抑制剂(T-20)、rvMIP+AZT、rvMIP+T-20对HIV-1前病毒载量的影响。结果所建立的荧光定量PCR体系的检测下限为101Copies,其标准曲线的相关系数为0.998 9,斜率为-4.925,截距为35.70;除6ng/ml的rvMIP、AZT、T-20外,三种药物处理后HIV-1前病毒载量均显著减小(P<0.05),并呈质量浓度依赖性;联合用药者前病毒载量显著低于单用rvMIP者(P<0.05),HIV-1前病毒载量rvMIP+T-20(rvMIP+AZT(rvMIP(T-20(AZT。结论本研究建立的SYBR Green Ⅰ荧光定量PCR检测方法具有灵敏、快速、准确及成本低廉等优点;与T-20或AZT联用可增强rvMIP对HIV-1前病毒的抑制作用。
基金Project(41472254)supported by the National Natural Science Foundation of China。
文摘Granite is usually composed of quartz,biotite,feldspar,and cracks,and the variation characteristics of these components could reflect the deformation and failure process of rock well.Taking granite as an example,the video camera was used to record the deformation and failure process of rock.The distribution of meso-components in video images was then identified.The meso-components of rock failure precursors were also discussed.Moreover,a modified LSTM(long short-term memory method)based on SSA(sparrow search algorithm)was proposed to estimate the change of meso-components of rock failure precursor.It shows that the initiation and expansion of cracks are mainly caused by feldspar and quartz fracture,and when the quartz and feldspar exit the stress framework,rock failure occurs;the second large increase of crack area and the second large decrease of quartz or feldspar area may be used as a precursor of rock failure;the precursor time of rock failure based on meso-scopic components is about 4 s earlier than that observed by the naked eye;the modified LSTM network has the strongest estimation ability for quartz area change,followed by feldspar and biotite,and has the worst estimation ability for cracks;when using the modified LSTM network to predict the precursors of rock instability and failure,quartz and feldspar could be given priority.The results presented herein may provide reference in the investigation of rock failure mechanism.
基金Projects(U22A20165, 52004289) supported by the National Natural Science Foundation of ChinaProjects(2022XJNY01, BBJ2024001) supported by the Fundamental Research Funds for the Central Universities,China。
文摘The damage and failure law of rock mass with holes is of great significance to the stability control of roadways. This study investigates the mechanical properties and failure modes of porous rock masses under cyclic loading, elucidates the acoustic emission (AE) characteristics and their spatial evolution, and establishes the interrelation among AE, stress, strain, time, and cumulative damage. The results reveal that the rock mass with holes and the intact rock mass show softening and hardening characteristics after cyclic loading. The plastic strain of the rock mass with holes is smaller than that of the intact rock mass, and the stress −strain curve shows hysteresis characteristics. Under uniaxial compression, the pore-bearing rock mass shows the characteristics of higher ringing count, AE energy, b-value peak, and more cumulative ringing count in the failure stage, while it shows lower characteristics under cyclic action. At the initial stage of loading, compared with the intact rock mass, the pore-containing rock mass shows the characteristics of a low b-value. The AE positioning and cumulative damage percentage are larger, and the AE positioning is denser around the hole. The specimen with holes is mainly shear failure, and the complete specimen is mainly tensile shear failure.
