To assess the role of dynamic contrast-enhanced MRI (DCE-MRI) in thediagnosis and differentiation of prostatic cancer (PC). Methods: Five volunteers, 36 patients withbenign prostatic hyperplasia (BPH) and 13 patients ...To assess the role of dynamic contrast-enhanced MRI (DCE-MRI) in thediagnosis and differentiation of prostatic cancer (PC). Methods: Five volunteers, 36 patients withbenign prostatic hyperplasia (BPH) and 13 patients with biopsy-proven prostate cancer underwentconventional MRI, DCE-MRI and delayed enhancement MRI. The value of the signal intensity in DCE-MRIwas measured and calculated to draw the time-signal intensity curve of the normal peripheral zone(PZ), the prostate cancer and the benign prostatic hyperplasia. Results: In DCE-MRI, the normalperipheral zone was enhanced mildly and slowly and the peak value was located in late phase. Theenhancement of the lesions in 36 patients with the benign prostatic hyperplasia was obvious in earlyphase and strengthened gradually, and then turned to decrease in late phase after peak value. Thelesions in 9 of 13 cases with prostate cancer were enhanced obviously in early phase and washed outrapidly, and the peak value was located in early phase, but the peak value was in mediate and latephase in the other 4 cases with diffuse lesion in the prostate on T_2WI. Conclusion: In DCE-MRI, theenhancement patterns of the normal peripheral zone, the prostate cancer and the benign prostatichyperplasia were significantly different. DCE-MRI was very useful in the diagnosis anddifferentiation of prostate cancer.展开更多
The transformer-2(tra-2) gene plays a key role in the regulatory hierarchy of sexual differentiation in somatic tissues and in the germline of Drosophila melanogaster.In this study,sequences and expression profiles of...The transformer-2(tra-2) gene plays a key role in the regulatory hierarchy of sexual differentiation in somatic tissues and in the germline of Drosophila melanogaster.In this study,sequences and expression profiles of tra-2 in the Chinese mitten crab Eriocheir sinensis were characterized.Four tra-2 isoforms,designated as Estra-2a,Estra-2b,Estra-2c,and Estra-2d,were isolated.They all contained an RNA-recognition motif(RRM) and a linker region,which shared high similarity with other reported tra-2s.Sequence analysis revealed that Estra-2a,Estra-2b and Estra-2c are encoded by the same genomic locus and are generated by alternative splicing of the pre-mRNA.Compared with the other three isoforms,Estra-2d lacks the RS2 domain.Quantitative real-time PCR showed that all four isoforms were highly expressed in the fertilized egg,and in the 2-4 cell and blastula stages compared with larval stages(P<0.01),suggesting their maternal origin in early embryonic developmental stages.Notably,Estra-2a was highly expressed in male somatic tissues,while Estra-2c was significantly highly expressed in the ovary.These results suggest that Estra-2c is involved in sexual differentiation of the Chinese mitten crab.Our findings provide basic information for further functional studies of the tra-2 gene/protein in this species.展开更多
Understanding the flame structure for different combustion in industries has drawn the increasing attention around the world.Particularly,for increasing the recent interest of using the hydrogen fuelled vehicles in re...Understanding the flame structure for different combustion in industries has drawn the increasing attention around the world.Particularly,for increasing the recent interest of using the hydrogen fuelled vehicles in recent world,structural analysis of flame in combustion chamber has attracted the attention of researchers.However,the high flame temperature and strong flame emissions increase the experimental difficulties,especially,in all kinds of intrusive measurement systems for determining the flame structures and flame temperatures.Therefore,a non-intrusive laser interferometer technique based on Shack-Hartmann optical system has been proposed to measure the thermal characteristic of a flame structure.In the present study,a low-stretched diffusion flame of methanol burner has been used.Shack-Hartmann optical system is a type of wave front sensor.It is commonly used in adaptive optical systems.It consists of an array of lenses to focus the image onto a photon sensor (photo-detector) at the focal plane and measures the wave front tilt.The major objective of the present study is to develop a laser interferometer measurement technique for analyzing the flame structure and its temperature propagation by measuring the density gradient of the flame.Optical interferometer technique is a potential candidate for the non-invasive measurement.In the present paper,a novel method for the measurement of density gradient in flame by using Shack-Hartmann optical system is proposed.A collimated laser beam that has been passed through the flame is tilted due to the density gradient inside the flame.A CCD camera (CCD photo sensor) has been used to observe the wave front tilts at the focal plane.展开更多
文摘To assess the role of dynamic contrast-enhanced MRI (DCE-MRI) in thediagnosis and differentiation of prostatic cancer (PC). Methods: Five volunteers, 36 patients withbenign prostatic hyperplasia (BPH) and 13 patients with biopsy-proven prostate cancer underwentconventional MRI, DCE-MRI and delayed enhancement MRI. The value of the signal intensity in DCE-MRIwas measured and calculated to draw the time-signal intensity curve of the normal peripheral zone(PZ), the prostate cancer and the benign prostatic hyperplasia. Results: In DCE-MRI, the normalperipheral zone was enhanced mildly and slowly and the peak value was located in late phase. Theenhancement of the lesions in 36 patients with the benign prostatic hyperplasia was obvious in earlyphase and strengthened gradually, and then turned to decrease in late phase after peak value. Thelesions in 9 of 13 cases with prostate cancer were enhanced obviously in early phase and washed outrapidly, and the peak value was located in early phase, but the peak value was in mediate and latephase in the other 4 cases with diffuse lesion in the prostate on T_2WI. Conclusion: In DCE-MRI, theenhancement patterns of the normal peripheral zone, the prostate cancer and the benign prostatichyperplasia were significantly different. DCE-MRI was very useful in the diagnosis anddifferentiation of prostate cancer.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A409)the Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology(No.2015ASKJ02)
文摘The transformer-2(tra-2) gene plays a key role in the regulatory hierarchy of sexual differentiation in somatic tissues and in the germline of Drosophila melanogaster.In this study,sequences and expression profiles of tra-2 in the Chinese mitten crab Eriocheir sinensis were characterized.Four tra-2 isoforms,designated as Estra-2a,Estra-2b,Estra-2c,and Estra-2d,were isolated.They all contained an RNA-recognition motif(RRM) and a linker region,which shared high similarity with other reported tra-2s.Sequence analysis revealed that Estra-2a,Estra-2b and Estra-2c are encoded by the same genomic locus and are generated by alternative splicing of the pre-mRNA.Compared with the other three isoforms,Estra-2d lacks the RS2 domain.Quantitative real-time PCR showed that all four isoforms were highly expressed in the fertilized egg,and in the 2-4 cell and blastula stages compared with larval stages(P<0.01),suggesting their maternal origin in early embryonic developmental stages.Notably,Estra-2a was highly expressed in male somatic tissues,while Estra-2c was significantly highly expressed in the ovary.These results suggest that Estra-2c is involved in sexual differentiation of the Chinese mitten crab.Our findings provide basic information for further functional studies of the tra-2 gene/protein in this species.
文摘Understanding the flame structure for different combustion in industries has drawn the increasing attention around the world.Particularly,for increasing the recent interest of using the hydrogen fuelled vehicles in recent world,structural analysis of flame in combustion chamber has attracted the attention of researchers.However,the high flame temperature and strong flame emissions increase the experimental difficulties,especially,in all kinds of intrusive measurement systems for determining the flame structures and flame temperatures.Therefore,a non-intrusive laser interferometer technique based on Shack-Hartmann optical system has been proposed to measure the thermal characteristic of a flame structure.In the present study,a low-stretched diffusion flame of methanol burner has been used.Shack-Hartmann optical system is a type of wave front sensor.It is commonly used in adaptive optical systems.It consists of an array of lenses to focus the image onto a photon sensor (photo-detector) at the focal plane and measures the wave front tilt.The major objective of the present study is to develop a laser interferometer measurement technique for analyzing the flame structure and its temperature propagation by measuring the density gradient of the flame.Optical interferometer technique is a potential candidate for the non-invasive measurement.In the present paper,a novel method for the measurement of density gradient in flame by using Shack-Hartmann optical system is proposed.A collimated laser beam that has been passed through the flame is tilted due to the density gradient inside the flame.A CCD camera (CCD photo sensor) has been used to observe the wave front tilts at the focal plane.
