Objective： To observe the effects of moxibustion at different times on prostaglandin E_2（PGE_2）, prostaglandin F2α（PGF_（2α））and arginine vasopressin（AVP）, in the uterine tissues of rats with dysmenorrhea du...Objective： To observe the effects of moxibustion at different times on prostaglandin E_2（PGE_2）, prostaglandin F2α（PGF_（2α））and arginine vasopressin（AVP）, in the uterine tissues of rats with dysmenorrhea due to cold-dampness retention, and to explore the differences and possible mechanisms of moxibustion at different times in easing pain in dysmenorrhea due to cold-dampness retention.Methods： Forty-three female Wistar rats were randomly divided into a blank control group（n=7）, a model group（n=9）,a pre-moxibustion group（n=9）, an immediate-moxibustion group（n=9） and a pre-moxibustion plus immediatemoxibustion group（n=9）. Rat models of primary dysmenorrhea due to cold-dampness retention were established using（0±1） ℃ ice water immersion method combined with injection of estradiol benzoate for 10 d, followed by injection of oxytocin on the 11 th day. Rats in the 3 intervention groups received moxibustion to Shenque（CV 8） and Guanyuan（CV 4）, 10 min for each acupoint, once a day. Rats in pre-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling, for 3 continuous days; rats in immediate-moxibustion group were given one time mild moxibustion, immediately after injection of oxytocin on the 11 th day during modeling; rats in pre-moxibustion plus immediate-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling till immediately after injection of oxytocin on the 11 th day during modeling, for 4 continuous days. The level of PGF_（2α） in the rat uterine tissues was measured by enzyme-linked immunosorbent assay（ELISA）, and the levels of PGE_2 and AVP in rat uterine tissues were measured by radioimmunoassay.Results： Compared with the blank control group, the levels of PGF_（2α） and AVP, the PGF_（2α）/PGE_2 ratio in the model group were significantly increased（P〈0.01）, and the PGE_2 level was significantly decreased（P〈0.01） in the rat uterine tissues in the model group. Compared with the model group, the writhing latency was significantly prolonged, the writhing number and the total writhing score were all decreased in the pre-moxibustion group, the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group（all P〈0.01）; the levels of PGF_（2α） and AVP, and the PGF_（2α）/PGE_2 ratio were all significantly decreased（P〈0.05, P〈0.01）, and the PGE_2 level was significantly increased（P〈0.01） in the rat uterine tissues of the 3 treatment groups. Compared with the pre-moxibustion group, the writhing number and the total writhing score were all decreased in the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group（all P〈0.01）, the writhing latency was significantly prolonged in the pre-moxibustion plus immediate-moxibustion group（P〈0.01）; the levels of PGF_（2α） and PGF_（2α）/PGE_2 ratio were significantly decreased（P〈0.05, P〈0.01）, and the PGE_2 level was significantly increased（P〈0.01） in rat uterine tissues in the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group. Compared with the immediate-moxibustion group, the writhing latency was significantly prolonged and the writhing number wasdecreased（all P〈0.05）, and the total writhing score was decreased（P〈0.01） in the pre-moxibustion plus immediatemoxibustion group; the PGF_（2α） level and the PGF_（2α）/PGE_2 ratio were significantly decreased（P〈0.01）, and the PGE_2 level was significantly increased（P〈0.01） in rat uterine tissues in the pre-moxibustion plus immediate-moxibustion group.Conclusion： Moxibustion at different times all can produce obvious analgesic effects on dysmenorrhea due to cold-dampness retention in rats, and pre-moxibustion plus immediate-moxibustion ranks the top. The mechanism of this analgesic effect may be via the regulation of abnormal PGF_（2α）, PGE_2 and AVP levels, to effectively inhibit the spastic contraction of uterine smooth muscle in dysmenorrhea rat, thereby improving the ischemia and hypoxia in uterus.展开更多
To investigate the effect of Qihuang herbal warm bag on primary dysmenorrhea. Methods： Thirty-two patients with primary dysmenorrhea were selected as subjects and treated by compression with the herbal warm bag for t...To investigate the effect of Qihuang herbal warm bag on primary dysmenorrhea. Methods： Thirty-two patients with primary dysmenorrhea were selected as subjects and treated by compression with the herbal warm bag for two months. The curative effect was evaluated, and prostaglandin F2a（PGF2a） and 13-endorphin（13-EP） were measured before and after treatment. Results： The herbal warm bag had positive effects on dysmenorrhea at different degrees and could decrease the contents of blood PGF2a and β-EP. Conclusion： The herbal warm bag is quite effective for primary dysmenorrhea possibly by decreasing serum PGF2a and increasing serum β-EP.展开更多
基金supported by Science and Technology Support Project of Hebei Provincial Administration of Traditional Chinese Medicine,No.2015089Science and Technology Research Project of Universities in Hebei Province,No.QN2015027Undergraduate Student Innovation Project of Hebei Province Education Office,No.201514432017~~
文摘Objective： To observe the effects of moxibustion at different times on prostaglandin E_2（PGE_2）, prostaglandin F2α（PGF_（2α））and arginine vasopressin（AVP）, in the uterine tissues of rats with dysmenorrhea due to cold-dampness retention, and to explore the differences and possible mechanisms of moxibustion at different times in easing pain in dysmenorrhea due to cold-dampness retention.Methods： Forty-three female Wistar rats were randomly divided into a blank control group（n=7）, a model group（n=9）,a pre-moxibustion group（n=9）, an immediate-moxibustion group（n=9） and a pre-moxibustion plus immediatemoxibustion group（n=9）. Rat models of primary dysmenorrhea due to cold-dampness retention were established using（0±1） ℃ ice water immersion method combined with injection of estradiol benzoate for 10 d, followed by injection of oxytocin on the 11 th day. Rats in the 3 intervention groups received moxibustion to Shenque（CV 8） and Guanyuan（CV 4）, 10 min for each acupoint, once a day. Rats in pre-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling, for 3 continuous days; rats in immediate-moxibustion group were given one time mild moxibustion, immediately after injection of oxytocin on the 11 th day during modeling; rats in pre-moxibustion plus immediate-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling till immediately after injection of oxytocin on the 11 th day during modeling, for 4 continuous days. The level of PGF_（2α） in the rat uterine tissues was measured by enzyme-linked immunosorbent assay（ELISA）, and the levels of PGE_2 and AVP in rat uterine tissues were measured by radioimmunoassay.Results： Compared with the blank control group, the levels of PGF_（2α） and AVP, the PGF_（2α）/PGE_2 ratio in the model group were significantly increased（P〈0.01）, and the PGE_2 level was significantly decreased（P〈0.01） in the rat uterine tissues in the model group. Compared with the model group, the writhing latency was significantly prolonged, the writhing number and the total writhing score were all decreased in the pre-moxibustion group, the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group（all P〈0.01）; the levels of PGF_（2α） and AVP, and the PGF_（2α）/PGE_2 ratio were all significantly decreased（P〈0.05, P〈0.01）, and the PGE_2 level was significantly increased（P〈0.01） in the rat uterine tissues of the 3 treatment groups. Compared with the pre-moxibustion group, the writhing number and the total writhing score were all decreased in the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group（all P〈0.01）, the writhing latency was significantly prolonged in the pre-moxibustion plus immediate-moxibustion group（P〈0.01）; the levels of PGF_（2α） and PGF_（2α）/PGE_2 ratio were significantly decreased（P〈0.05, P〈0.01）, and the PGE_2 level was significantly increased（P〈0.01） in rat uterine tissues in the immediate-moxibustion group and the pre-moxibustion plus immediate-moxibustion group. Compared with the immediate-moxibustion group, the writhing latency was significantly prolonged and the writhing number wasdecreased（all P〈0.05）, and the total writhing score was decreased（P〈0.01） in the pre-moxibustion plus immediatemoxibustion group; the PGF_（2α） level and the PGF_（2α）/PGE_2 ratio were significantly decreased（P〈0.01）, and the PGE_2 level was significantly increased（P〈0.01） in rat uterine tissues in the pre-moxibustion plus immediate-moxibustion group.Conclusion： Moxibustion at different times all can produce obvious analgesic effects on dysmenorrhea due to cold-dampness retention in rats, and pre-moxibustion plus immediate-moxibustion ranks the top. The mechanism of this analgesic effect may be via the regulation of abnormal PGF_（2α）, PGE_2 and AVP levels, to effectively inhibit the spastic contraction of uterine smooth muscle in dysmenorrhea rat, thereby improving the ischemia and hypoxia in uterus.
文摘To investigate the effect of Qihuang herbal warm bag on primary dysmenorrhea. Methods： Thirty-two patients with primary dysmenorrhea were selected as subjects and treated by compression with the herbal warm bag for two months. The curative effect was evaluated, and prostaglandin F2a（PGF2a） and 13-endorphin（13-EP） were measured before and after treatment. Results： The herbal warm bag had positive effects on dysmenorrhea at different degrees and could decrease the contents of blood PGF2a and β-EP. Conclusion： The herbal warm bag is quite effective for primary dysmenorrhea possibly by decreasing serum PGF2a and increasing serum β-EP.
