In order to study the mechanism of bolt support and the behavior of strata in a coal roadway under tectonic stress,deformation and destruction of a roof,floor and sides were studied using an experiment in similarity s...In order to study the mechanism of bolt support and the behavior of strata in a coal roadway under tectonic stress,deformation and destruction of a roof,floor and sides were studied using an experiment in similarity simulation.We also studied the mechanism and types of bolt support functions in the coal roadway.The results show that with an increase in horizontal tectonic stress,the strata in the roof and floor of the roadway gradually separate and become shear failure areas.Coal in side walls moves,but its integrity remains intact.Side bolts are mainly affected by tension and roof bolts by the effect of shear.展开更多
Different from canonical ubiquitin-like proteins, Hub1 does not form covalent conjugates with substrates but binds proteins noncovalently. In Socchoromyces cerevisioe, Hub1 associates with spUceosomes and mediates alt...Different from canonical ubiquitin-like proteins, Hub1 does not form covalent conjugates with substrates but binds proteins noncovalently. In Socchoromyces cerevisioe, Hub1 associates with spUceosomes and mediates alternative splicing of SRCI, without affecting pre-mRNA splicing generaity. Human Hub1 is highty similar to its yeast homotog, but its cellular function remains largely unexplored. Here, we show that human Hub1 binds to the spliceosomal protein Snu66 as in yeast; however, unlike its 5. cerevisioe homolos, human Hub1 is essential for viability. Prolonged in vivo depletion of human Hub1 leads to various cellular defects, including splicing speckle abnormalities, partial nuclear retention of mRNAs, mitotic catastrophe, and consequently cell death by apoptosis. Early consequences of Hub1 depletion are severe splicing defects, however, only for specific splice sites leading to exon skipping and intron retention. Thus, the ubiquitin-iike protein Hub1 is not a canonlcal spliceosomal factor needed generally for splicing, but rather a modulator of spliceosome performance and facilitator of alternative splicing.展开更多
基金the Research Fund of the State Key Laboratory of Coal Resources and Mine Safety of the China University of Mining & Technology (No.08kf11)
文摘In order to study the mechanism of bolt support and the behavior of strata in a coal roadway under tectonic stress,deformation and destruction of a roof,floor and sides were studied using an experiment in similarity simulation.We also studied the mechanism and types of bolt support functions in the coal roadway.The results show that with an increase in horizontal tectonic stress,the strata in the roof and floor of the roadway gradually separate and become shear failure areas.Coal in side walls moves,but its integrity remains intact.Side bolts are mainly affected by tension and roof bolts by the effect of shear.
文摘Different from canonical ubiquitin-like proteins, Hub1 does not form covalent conjugates with substrates but binds proteins noncovalently. In Socchoromyces cerevisioe, Hub1 associates with spUceosomes and mediates alternative splicing of SRCI, without affecting pre-mRNA splicing generaity. Human Hub1 is highty similar to its yeast homotog, but its cellular function remains largely unexplored. Here, we show that human Hub1 binds to the spliceosomal protein Snu66 as in yeast; however, unlike its 5. cerevisioe homolos, human Hub1 is essential for viability. Prolonged in vivo depletion of human Hub1 leads to various cellular defects, including splicing speckle abnormalities, partial nuclear retention of mRNAs, mitotic catastrophe, and consequently cell death by apoptosis. Early consequences of Hub1 depletion are severe splicing defects, however, only for specific splice sites leading to exon skipping and intron retention. Thus, the ubiquitin-iike protein Hub1 is not a canonlcal spliceosomal factor needed generally for splicing, but rather a modulator of spliceosome performance and facilitator of alternative splicing.
