Measurement and Study of Tyrosinase Activity in Potato

[Objective] The paper was to study the activity of tyrosinase,so as to provide reference for preventing browning of potatoes as well as fruits and vegetables containing tyrosinase.[Method] With potato as raw material and Na2HPO4-HCl in buffer solution（pH=7.2）as system,spectrophotometer was used to measure the absorbance of potato extract at 480nm,the curve was established,and the activity of tyrosinase was obtained.[Result] The changes of data were little under the above conditions,and the stability of tyrosinase activity was high.[Conclusion] Using spectrophotometry to determine the tyrosinase activity in potato is simple with high accuracy.

Study on Extraction and Catalytic Activity of Tyrosinase

Objective] Tyrosinase is a kind of polyphenol oxidase used widely, and the study of tyrosinase attracts extensive attention. [Method] ln this experiment, A conversion kinetic curve of dopa solution was obtained using absorbance of potato extract at 480 nm in Na2HPO4-HCl buffer system at a pH value of 7.2 with the vari-ation rate of absorbance against time （ΔA/Δt） as reaction rate, and the activity of tyrosinase could thus be calculated. [Result] The results showed that at the wave-length, in the Na2HPO4-HCl buffer system with a pH value at 7.2, there was little in-terference to the system, the data showed smal fluctuations, and the activity of ty-rosinase was high and stable, further providing a theoretical foundation for the de-termination and study of factors influencing tyrosinase activity. [Conclusion] The catalytic activity of tyrosinase was studied and determined with buffer solution as substrate with high accuracy and good effect.

High Pressure Structural Instability and Thermal Properties of Rutile TiO2 from First-principles

We report a first-principles calculation to investigate the structural instability of rutile TiO2. The high pressure structural parameters are well reproduced. The calculated phonon disper-sion curves agree with experiments at zero pressure. Under compression, we capture a large softening around Γ point, which indicates the structural instability. From the high pressure elastic constants, we find that the rutile TiO2 is unstable when the applied pressure is larger than 17.7 GPa. Within the quasi-harmonic approximation, the thermal equation of state, thermal expansion oefficient, bulk modulus, and entropy are well reproduced. The thermal properties confirm the available experimental data and are extended to a wider pressure and temperature range.

飞行仿真加载控制系统的研究和应用

本文对飞行仿真加载、配平控制系统的技术要求、方案选择作了分析,重点阐述了驾驶杆俯仰加载、配平控制系统的基本结构、工作过程及融合设计计算,并对该系统的发展方案作了探索。

Properties of failure mode and thermal damage for limestone at high temperature

The mechanical properties of limestone such as the stress-strain curve, the variable characteristics of peak strength and the modulus of elasticity of limestone were studied under the action of temperatures ranging from room temperature to 800 °C.Our results show that:1) the temperature has not clear effect on the mechanical properties of limestone from room temperature to 600 °C.However, the mechanical properties of limestone deteriorate rapidly when the temperature is above 600 °C.In this case, the peak stress and modulus of elasticity decrease rapidly.When the temperature reaches 800 °C, the entire process, showing the stress-strain curve is displayed indicating an obvious state of plastic-deformation;2) the failure mode of limestone shows the breakdown of tensile strength from room temperature to 600 °C, as well as the compress shearing damage over 600 °C;3) combining our test results with the concept of thermal damage, a thermal damage equation was derived.

MPC-based path tracking with PID speed control for high-speed autonomous vehicles considering time-optimal travel

In order to track the desired path as fast as possible,a novel autonomous vehicle path tracking based on model predictive control(MPC)and PID speed control was proposed for high-speed automated vehicles considering the constraints of vehicle physical limits,in which a forward-backward integration scheme was introduced to generate a time-optimal speed profile subject to the tire-road friction limit.Moreover,this scheme was further extended along one moving prediction window.In the MPC controller,the prediction model was an 8-degree-of-freedom(DOF)vehicle model,while the plant was a 14-DOF vehicle model.For lateral control,a sequence of optimal wheel steering angles was generated from the MPC controller;for longitudinal control,the total wheel torque was generated from the PID speed controller embedded in the MPC framework.The proposed controller was implemented in MATLAB considering arbitrary curves of continuously varying curvature as the reference trajectory.The simulation test results show that the tracking errors are small for vehicle lateral and longitudinal positions and the tracking performances for trajectory and speed are good using the proposed controller.Additionally,the case of extended implementation in one moving prediction window requires shorter travel time than the case implemented along the entire path.

Strengthening and stress drop of ultrafine grain aluminum after annealing

Ultrafine grain pure aluminum was produced by equal channel angular pressing and cold rolling, the deformed aluminum was annealed at 200 ℃ for 1 h. The tensile curves of deformed and annealed aluminum show that yield strength of deformed aluminum increases by 100%-300% and its elongation decreases by about 20%. After low temperature annealing, strength of annealed aluminum increases by 20% and elongation decreases by over 50%, the recovery of dislocations may be the main cause of annealing strengthening. In addition, there is an abrupt stress drop in the tensile curves of annealed aluminum and the formation of shear band is responsible for it.

Dynamics of phase transformation of Cu-Ni-Si alloy with super-high strength and high conductivity during aging

The precipitation behaviors of the Cu-Ni-Si alloys during aging were studied by analyzing the variations of electric conductivity.The Avrami-equation of phase transformation kinetics and the Avrami-equation of electric conductivity during aging were established for Cu-Ni-Si alloys,on the basis of linear relationship between the electric conductivity and the volume fraction of precipitates,and the calculation results coincide well with the experiment ones.The transformation kinetics curves were established to characterize the aging process.The characteristics of precipitates in the supersaturated solid solution alloy aged at 723 K were established,and the results show that the precipitates areβ-Ni3Si andδ-Ni2Si phases.

Hot deformation behavior and constitutive relationship of Q420qE steel

Isothermal compression tests at temperatures from 1 273 to l 423 K and strain rates from 0.1 to 10 s-q were carried out to investigate the flow behaviors of Q420qE steel. Stress-strain data collected from the tests were employed to establish the constitutive equation, in which the influence of strain was incorporated by considering the effect of strain on material constants Q, n, a, and lnA. The results show that the flow stress curves are dependent on the strain, strain rate and deformation temperature. They display typical dynamic recrystallization behavior and consist of three stages, i.e., hardening stage, softening stage and steady stage. The flow stress decreases with increasing the deformation temperature and decreasing the strain rate. In addition, the flow stress data predicted by the proposed constitutive model agree well with the corresponding experimental results, and the correlation coefficient and the average absolute relative error between them are 0.990 3 and 3.686%, respectively.

Experimental study on the effects of big particles physical characteristics on the hydraulic transport inside a horizontal pipe

This paper presents an experimental study of the physical characteristic effects of large particles on hydraulic transport in a horizontal pipe.The particles are spherical and are large with respect to the diameter of the pipe(8%,10%,16% and 25%).Experiments were done to test the important parameters in solid transport(pressure,velocity,etc.).As a result,the relationship between the pressure gradient forces and the mixture velocity was substantially different from the pure liquid flow.However,in a single-phase flow a monotonous behavior of the pressure drop curve is observed,and the curve of the solid particle flow attains its minimum at the critical velocity.The regimes are characterized with differential pressure measurements and visualizations.

Numerical simulation of the pulsing air separation field based on CFD

The flow field of pulsing air separation is normally in an unsteady turbulence state.With the application of the basic principles of multiphase turbulent flows,we established the dynamical computational model,which shows a remarkable variation of the unstable pulsing air flow field.CFD(computational fluid dynamics) was used to conduct the numerical simulation of the actual geometric model of the classifier.The inside velocity of the flowing fields was analyzed later.The simulation results indicate that the designed structure of the active pulsing air classifier provided a favorable environment for the separation of the particles with different physical characters by density.We shot the movement behaviors of the typical tracer grains in the active pulsing flow field using a high speed dynamic camera.The displacement and velocity curves of the particles in the continuous impulse periods were then analyzed.The experimental results indicate that the effective separation by density of the particles with the same settling velocity and different ranges of the density and particle size can be achieved in the active pulsing airflow field.The experimental results provide an agreement with the simulation results.

Inproved grade length limitation of freeways

A method of ascertaining grade length limitation of freeways in mountain terrain is presented. The relationship models between 15 th percentile speeds and grades were built through the surveys and analyses of operation speeds on 7 typical sections of 3 freeways in mountain terrain. Using 15 thpercentile and 85 th percentile as speed limitations, the methods of determining admitted velocities were put forward according to the grades. Deceleration distances of longitudinal slopeways were analyzed utilizing the theories of vehicle. According to the results of analysis, grade length limitation was loosed. Finally the values of grade length limitation for freeways were put forward. The results could be used as references for freeway design.

Numerical Simulation of Coseismic Response and Its Mechanism of Well Water Temperature to Farfield Strong Earthquakes1

Heat flow is inevitably accompanied by temperature change,thus,the water temperature coseismic response during earthquake activity should also obey the laws of thermodynamics.Taking the M S8.0 Wenchuan,Sichuan,China earthquake and the M9.0Tohoku,Japan earthquake as an example,and based on the data of water temperature coseismic responses observed in well ZK26 in Haikou,Hainan Province,China,we investigate the relationship between well water temperature change and heat transfer in the coseismic response process and the relevant thermodynamic mechanism by using the numerical simulation method for thermodynamic equations.Then,through forward modeling,we obtain several simulation curves of water temperature change in response to earthquakes along the well depth at different times.The simulated curves of water temperature change approximately fit the observed curves.Consequently,based on the variation of temperature,we find that the modes of well water temperature coseismic response( ascending,descending or stable) are related to factors such as the location of sensors,distribution and location of heat sources,the span between sensors and heat sources.

Experimental Confirmation on the Calibration Curves for Preston＇s Method

The Preston＇s method is considered as one of the most commonly employed methods to measure the wall shear stress. However, it is only possible to determine the wall shear stress from measured pressure differences of the Preston tube and undisturbed static pressure, combined with calibration curves, which depend on the Preston tube diameter, fluid density, and viscosity. Since its invention, no significant advancement in theory has been made, and calibration curves proposed by Preston, Patel and Bechert are still in use. In the present study, a need to measure surface shear stress over a circular cylinder prompted us to develop our original Preston tube system. The developed system has been calibrated by measuring the wall shear stress in the fully developed turbulent flow regime in a circular pipe. The present results generally confirm the previously reported calibration curves. A slight modification of the coefficients in the calibration equation shows further improvement.

Dynamic recrystallization behavior and kinetics of high strength steel

The dynamic recrystallization behavior of high strength steel during hot deformation was investigated.The hot compression test was conducted in the temperature range of 950-1150 °C under strain rates of 0.1,1 and 5 s-1.It is observed that dynamic recrystallization(DRX) is the main flow softening mechanism and the flow stress increases with decreasing temperature and increasing strain rate.The relationship between material constants(Q,n,α and ln A) and strain is identified by the sixth order polynomial fit.The constitutive model is developed to predict the flow stress of the material incorporating the strain softening effect and verified.Moreover,the critical characteristics of DRX are extracted from the stress-strain curves under different deformation conditions by linear regression.The dynamic recrystallization volume fraction decreases with increasing strain rate at a constant temperature or decreasing deformation temperature under a constant strain rate.The kinetics of DRX increases with increasing deformation temperature or strain rate.

论肖邦叙事曲体裁的悲剧——戏剧性及其叙事意义

肖邦首创了作为器乐体裁的叙事曲（Ballade）,是其最富表现力和诗意内涵的杰作。本文试图论述这一体裁的悲剧—戏剧性（1）及其深层的叙事意义。通过将＂戏剧性＂进行可视化呈现以及将＂叙事性＂抽象化为＂原型＂,笔者试图在体裁层面对上述两种特质予以归纳和阐释。

Biomathematical analysis of carbon nanotubes due to ciliary motion

In this paper, the consequences of cilia motion are reflected by the CNTs nanoparticles. The problem is expressed in a symmetric channel with ciliated walls. Exact solutions of the governing flow problem are obtained for pressure gradient, temperature and velocities of the fluid. Streamlines for the velocity profile are plotted to discuss the trapping phenomenon.

Effects of blade rotation angle deviations on mixed-flow pump hydraulic performance

By model test and numerical simulation, this paper analyzed the effects of different blades with varying rotation angle deviations on the hydraulic performance of a mixed-flow pump. It was found that when some blades had rotation angle deviations, the hydraulic performance curves of the mixed-flow pump would move. With a positive deviation, the curves moved towards the large flow rate; with a negative deviation, the curves moved towards the small flow rate. When some blades had rotation angle deviations, the symmetry and uniformity of the pressure distribution inside the mixed-flow pump flow passage both decreased; the larger the deviation, the greater the decrease. When a single blade had a large rotation angle deviation, a rather clear low pressure area was formed, lowering the cavitation performance. When two adjacent blades changed simultaneously, under the small flow rate condition, adverse pressure gradient and flow separation occurred in the flow field, and a hump appeared in the head curve and the operation stability of the mixed-flow pump dropped significantly. Near the best efficiency point（BEP）, the simultaneous change of two alternate blades produced a more significant change of pressure in the flow passage, with an even larger area. Compared to the effect of two adjacent blades, two alternate blades, when changed simultaneously, made the mixed-flow pump slightly less efficient, but with a flatter efficiency curve and relatively wider high efficiency area. By fitting the test results, a functional relation among the BEP of the mixed-flow pump QBEP, the number of deviated blades N, and blade rotation angle deviation α was established, thus realizing an effective prediction of the BEP of the mixed-flow pump when blade rotation angles have deviations.

Estimation of the J-resistance curve for Cr2Ni2MoV steel using the modified load separation parameter S_(pb) method

Based on load separation theory, the load separation parameter Spb method is an effective approach for estimating the J-resistance curve from records of load versus displacement directly, using one sharp cracked specimen and an additional reference blunt cracked specimen. However, the effect of the reference blunt cracked specimen on J-resistance determination was not explicitly considered in past work. In this paper, a modified load separation parameter Spb method was developed to eliminate this effect, and then a unique estimation of instantaneous crack length for one sharp cracked specimen could be obtained. Furthermore, a forced blunting calibration method was also adopted to determine the instantaneous crack length in the load inseparable region, referring to a normalization method. Experiments on steam turbine rotator steel Cr2Ni2MoV were carried out to estimate J-resistance curves using an unloading compliance method. By removing unload and reload data from load-displacement records, the J-resistance curve for the same sharp cracked specimen was estimated using the modified separation parameter Spb method. The results indicate that the modified Spb method completely eliminates the effect of the reference blunt cracked specimen on the instantaneous crack length determination of the sharp cracked specimen. However, different J-resistance curves in a small range of crack extension are present when different blunting coefficients are used in the blunting line equation. The J-resistance curve obtained from the modified Spb method agrees well with that obtained from the compliance method.