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天津地区鸡源沙门氏菌的基因分型研究 被引量:3
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作者 王骁 赵处敏 +4 位作者 康青 杜婷 李萍 杜欣军 王硕 《食品安全质量检测学报》 CAS 北大核心 2022年第14期4487-4493,共7页
目的研究并评估几种分子分型方法对亲缘关系相近鸡源沙门氏菌的分辨能力。方法针对2015—2016年从天津市大型市场和超市中分离的106株沙门氏菌及其全基因组测序结果,分析了沙门氏菌的血清型、序列类型(sequence types,STs),并通过脉冲... 目的研究并评估几种分子分型方法对亲缘关系相近鸡源沙门氏菌的分辨能力。方法针对2015—2016年从天津市大型市场和超市中分离的106株沙门氏菌及其全基因组测序结果,分析了沙门氏菌的血清型、序列类型(sequence types,STs),并通过脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)、核心基因组多位点序列分型(core genome multilocus sequence typing,cgMLST)以及一种基于59个基因的分型方法对沙门氏菌进行分子分型研究。结果106株沙门氏菌共分为8种血清型和8个ST型;PFGE分型方法将沙门氏菌分为7个集群;cgMLST将沙门氏菌分为8个集群;59个基因的分型方法将亲缘关系相近的分离株分为9个集群。结论肠炎沙门氏菌为这些沙门氏菌中的优势亚型;cgMLST具有最高的分辨率;基于59个基因的分型方法可以满足对亲缘关系相近沙门氏菌的分型研究。 展开更多
关键词 沙门氏菌 脉冲场凝胶电泳 核心基因组多位点序列分型 功能基因分型
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Physiological Analysis of Two Arabidopsis thaliana Mutants in Response to CO2 被引量:11
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作者 宋玉伟 陈家宝 刘宗才 《Agricultural Science & Technology》 CAS 2009年第2期12-14,共3页
[Objective] The 15urpose was to seek for the different phenotypes between wild type and Arabidopsis Mutants in response to CO2. [Method] The epidermis bioassays and seed germination test were carried out to analyze th... [Objective] The 15urpose was to seek for the different phenotypes between wild type and Arabidopsis Mutants in response to CO2. [Method] The epidermis bioassays and seed germination test were carried out to analyze the physiological characteristics of two Arabidopsis mu- tants and their wild type. [Result] There existed distinct differences in stomata apertures, water loss and leaf temperature compared with wild type except for stomata density. In addition, seed germination test on the medium indicated that cdfl was insensitive to ABA, mannitol and NaCI, but cdsl performed contrary to cdil. [ Conclusion] There are some different physiological characteristics between wild type and mutants. 展开更多
关键词 Arabidopsis thaliana CO2 MUTANT
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A data structure and function classification based method to evaluate clustering models for gene expression data
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作者 易东 杨梦苏 +2 位作者 黄明辉 李辉智 王文昌 《Journal of Medical Colleges of PLA(China)》 CAS 2002年第4期312-317,共6页
Objective:To establish a systematic framework for selecting the best clustering algorithm and provide an evaluation method for clustering analyses of gene expression data. Methods: Based on data structure (internal in... Objective:To establish a systematic framework for selecting the best clustering algorithm and provide an evaluation method for clustering analyses of gene expression data. Methods: Based on data structure (internal information) and function classification (external information), the evaluation of gene expression data analyses were carried out by using 2 approaches. Firstly, to assess the predictive power of clusteringalgorithms, Entropy was introduced to measure the consistency between the clustering results from different algorithms and the known and validated functional classifications. Secondly, a modified method of figure of merit (adjust-FOM) was used as internal assessment method. In this method, one clustering algorithm was used to analyze all data but one experimental condition, the remaining condition was used to assess the predictive power of the resulting clusters. This method was applied on 3 gene expression data sets (2 from the Lyer's Serum Data Sets, and 1 from the Ferea's Saccharomyces Cerevisiae Data Set). Results: A method based on entropy and figure of merit (FOM) was proposed to explore the results of the 3 data sets obtained by 6 different algorithms, SOM and Fuzzy clustering methods were confirmed to possess the highest ability to cluster. Conclusion: A method based on entropy is firstly brought forward to evaluate clustering analyses.Different results are attained in evaluating same data set due to different function classification. According to the curves of adjust_FOM and Entropy_FOM, SOM and Fuzzy clustering methods show the highest ability to cluster on the 3 data sets. 展开更多
关键词 gene expression evaluation of clustering adjust- FOM ENTROPY
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