电针足三里穴对小肠运动功能障碍大鼠植物神经放电活动的影响

目的以经络-脏腑相关理论为指导,观察电针足三里穴对小肠运动抑制大鼠的植物神经的影响。方法雄性Wistar大鼠,随机分成空白对照组、模型对照组、电针治疗组。以静脉注射阿托品致小肠运动功能障碍为模型,电针单侧足三里穴,采用神经电生理技术,记录各组大鼠迷走神经和内脏大神经放电变化。结果空白对照组植物神经放电频率在观察期内无明显变化;模型对照组造模前后比较,造模后迷走神经放电频率明显减少(P<0.01),内脏大神经放电频率明显增高(P<0.01);电针治疗组电针后迷走神经放电频率明显增高(P<0.01),内脏大神经放电频率明显减少(P<0.01)。结论电针足三里穴对小肠植物神经放电的影响可能是引起小肠运动功能改变的途径之一。

功能性电针配合Bobath技术治疗脑卒中功能障碍疗效分析

目的分析功能性电针配合Bobath技术治疗脑卒中功能障碍的临床疗效。方法随机将脑卒中伴功能障碍患者80例分为治疗组和对照组。对照组进行Bobath技术治疗,治疗组给予功能性电针配合Bobath技术治疗。采用神经功能缺损、运动功能、日常生活活动能力评价改善情况。结果治疗组治疗后FMA评分、神经功能缺损评分和日常生活活动能力评分均优于对照组,差异有统计学意义(P<0.05)。结论利用功能性电针配合Bobath技术治疗脑卒中功能障碍,能够显著改善患者运动功能,提高其日常生活活动能力。

电针治疗对脑梗死偏瘫急性期运动功能的影响

目的:观察电针对脑梗死偏瘫急性期运动功能的影响。方法:将100例患者随机分成电针治疗观察组50例和体针治疗对照组50例,两组按传统体针取穴治疗,电针组加用电针治疗。结果:电针组与体针组之间,对运动功能Brunnstrom分级指数的影响,上肢和下肢均有显著差异(P<0.05)。结论:电针治疗对脑梗死的运动功能疗效优于传统体针。

电针对术后认知功能障碍模型大鼠外周炎性因子表达的影响

目的:研究电针对术后认知功能障碍(POCD)模型大鼠血清中炎性因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)表达的影响,探讨其对老年术后认知功能障碍的可能作用机制。方法:100只雌性SD大鼠,随机分为对照组、模型组、电针组、药物组,制备POCD模型,分别给予电针及药物治疗,酶联免疫吸附法(ELISA法)检测电针对POCD大鼠造模后1天、3天、7天血清中IL-1β、IL-6及TNF-α表达的影响。结果与结论:电针能通过减少IL-1β、IL-6和TNF-α表达的途径抑制炎症反应,保护外周环境,进而改善大鼠的认知能力。

“功能电针”配合运动疗法治疗中风后肩关节半脱位

围绕中风后肩关节半脱位(GHS)患者,采用“功能电针”与运动疗法相联合的方案来治疗,评定其效果与价值。方法 选取本院在特定时间节点内(2013年6月-2015年6月)收治的中风后GHS患者(100例),将其实施分组(2组,所用方法是随机数字表法,每组皆为50例),对照组采用普通针刺配合运动疗法,治疗组给予功能电针配合运动疗法治疗,对比两组肩峰到肱骨头间距(AHI)评分、临床疗效。结果 治疗组治疗总有效率(98.00%)相比对照组(84.00%),显著偏高(P<0.05)。治疗组治疗后的AHI评分较对照组低(P<0.05)。结论 针对经检查确诊的中风后GHS患者,采取“功能电针”配合运动疗法的方案治疗,效果好,能显著改善肩关节脱位情况。

神经肌肉治疗仪配合电针治疗周围性面瘫50例

８０例周围性面瘫患者分为两组，电针治疗组３０例，多功能神经肌肉治疗仪配合电针治疗组５０例，两组选择相同的针刺操作方法和穴位地仓、颊车、人迎、合谷、下关、四白、阳白，其结果如下：１、电针组痊愈１８例，显效５例，好转３例，无效４例。２、多功能电针治疗组痊愈４０例，显效５例，好转４例，无效１例。经统计学处理两组存在显著差异（Ｐ＜０．０５）并且多功能电针治疗组疗程比电针组短，见效快。

EFFECTS OF LOW-FREQUENCY ELECTROACUPUNCTURE ON THE IMMUNOLOGIC FUNCTION IN MORPHINE DEPENDENCE RATS

Objective: To observe the effect of low-frequency electroacupuncture (EA) on the immunologic function in morphine dependence rats. Methods: Forty SD rats were used in this study. Morphine-dependence model was established by intraperitoneal injection of morphine hydrochloride continuously for 5 days and hastened by administration (i.p) of Naloxone. These rats were randomly divided into control, model, EA and auto-demorphinization groups with 10 cases being in each group. In EA group, "Guanyuan"(CV 4),"Mingmen"(GV 4), etc. were punctured and stimulated electrically. Positive T lymphocyte subgroups, CD + 4 and CD + 8 in the peripheral blood were detected with fluorescence immuno-assay. Results: In model group, serum percentage of CD + 4 and CD + 4/CD + 8 decreased considerably in comparison with those of control group (P<0.01); while in EA group, CD + 4 level and CD + 4/CD + 8 increased significantly compared with those of model group (P<0.01); and no significant differences were found between auto-demorphinization group and model group and between EA and control groups in these two indexes. Conclusion: Low-frequency EA can promote the restoration of the immune function of morphine dependence rats.

Effects of electroacupuncture on the proprioception of athletes with functional ankle instability

Objective To observe the efficacy differences between electroacupuncture （EA） and physiotherapy （PT） on the proprioception of athletes with functional ankle instability （FAI）. Methods Fifty athletes with FAI were randomly divided into an EA group and a PT group. The EA group was treated with EA at Jiexi （解溪 ST 41）, Kunlun （昆仑 BL 60）, Qjuxu （丘墟 GB 40） and Ashi points, and the PT group was treated with low frequency electrical stimulation and infrared radiation at medial malleolus and lateral malleolus, thrice each week for consecutive 8 weeks. The joint position sense： active （JPSA）, joint position sense： passive （JPSP） and Kinaesthesia （KT） were assessed at the ankle by use of Biodex System isokinetic dynamometer to test the proprioception before and after the treatment. Results In the EA group, JPSA of 11.09°±3.1° and JPSP of 9.67°±2.8° before the treatment were reduced to 9.14°±4.0° and 6.89°±3.3° respectively after the treatment, and there were statistically significant differences in comparison between pre- and post-treatment （all P〈0.05）. Comparison between the EA group and the PT group, there were significant differences in JPSA and JPSP （all P〈0.05）, no significant difference in KT （P〉0.05）. There were no significant differences in the indices of JPSA, JPSP and KT in the PT group after the treatment than those before the treatment （all P〉0.05）. Conclusion EA can effectively improve the proprioception of athletes with FAI and achieve a better efficacy as compared with the conventional physiotherapy.

Effects of different acupuncture-moxibustion methods on the plasma SP content and expression of colonic SP mRNA of rats with functional constipation

Objective To explore the effects of different acupuncture-moxibustion methods on the plasma substance P（SP） content,and the expression of colonic SP m RNA of the rats with functional constipation.Methods Sixty healthy male SD rats were divided into six groups according to the random number table,including blank control group（group B,n=8）,model group（group M,n=11）,drug control group（group D,n=8）,filiform needle group（group F,n=11）,electroacupuncture group（group E,n=11） and moxibustion group（group MB,n=11）.The rats in group B and group M accepted no intervention,gavage with cisapride suspension was conducted in group D,and electroacupuncture,filiform needling and moxibustion therapies were carried out in group E,group F and group MB for 6 days.After treatment,the stool quantity and fecal moisture content during 24 h of the rats were observed in each group,colon tissue structure and the acidic mucus in mucosa layer were observed,plasma SP content was detected and the expression of colonic SP m RNA was detected.Results compared with group B,the stool quantity and fecal moisture content during 24 h of the rats in group M decreased significantly（P0.01）;compared with group M,the stool quantity during 24 h of the rats in each group increased（P0.05 or 0.01）,and the fecal moisture content during 24 h of the rats in each group also increased significantly except group MB（P0.01）;compared with group D,the fecal moisture content during 24 h of the rats in group MB decreased（P0.01）;according to the comparison among the groups with different acupuncture-moxibustion methods,there was no difference in the stool quantity during 24 h of the rats in each group（P0.05）,the fecal moisture contents during 24 h of the rats in group F and group E were higher than that of group MB（P0.01）,and there was no difference between group F and group E（P0.05）.HE staining of colon tissue of rats showed that the structure was normal and complete,and there was no difference among each group.PAS staining showed that the acidic mucus level of the rats in group M decreased significantly when compared with group B;compared with group M,the acidic mucus level of the rats in group D,fgroup F,group E and group MB increased in varying degrees.Compared with group B,the plasma SP content of the rats in group M decreased（P0.05）;compared with group M,the plasma SP contents of the rats in group F and group MB increased（P0.01）;the plasma SP contents of the rats in group F,group MB and group E were higher than that of group D（P0.05 or 0.01）;according to the comparison among the groups with different acupuncture-moxibustion methods,the plasma SP content of the rats in group F was higher than that of group MB and electro-acupuncture group（P0.05）,and there was no difference between group MB and group E.Compared with group B,the expression level of colonic SP m RNA of the rats in group M elevated（P0.05）;compared with group M,the expression level of colonic SP m RNA of the rats in the four intervention groups declined（P0.05）;compared with drug group D,there was no difference among group F,group E and group MB（P0.1）;according to the comparison among the groups with different intervention methods,the expression level of colonic SP m RNA of the rats in group MB was lower than that of group F（P0.05）.Conclusion The different efficacy of different acupuncture-moxibustion methods in treatment of functional constipation may be related with the mechanism of regulation of plasma SP content and the expression level of colonic SP m RNA.

Effects of different frequencies of electro-acupuncture at Shuigou(GV 26) on recovery of motor function in rats with focal cerebra ischemic injury

OBJECTIVE:To investigate the effects of different frequencies of electro-acupuncture at Shuigou(GV 26) on the latent period and wave amplitude of motor evoked potentials(MEPs) in rats with focal cerebral infarction.METHODS:Fifty healthy male Wistar rats were randomly divided into five groups:controls,model,2 Hz Shuigou,50 Hz Shuigou and 100 Hz Shuigou.There were 10 rats in each group.Using a modification of a technique for middle cerebral artery occlusion,focal cerebral ischemic injury was induced in all rats except those in the control group.The rats in the control group received no treatment.After behavioral deficit had been evaluated using the Zausinger 6-point neurological function score,therats in the Shuigou groups underwent acupuncture and continuous wave stimulation at a frequency of 2 Hz,50 Hz or 100 Hz(intensity 1 mA) for 10 min twice daily for 3 days.The control and model groups underwent no intervention.Zausinger 6-point neurological function score and MEPs were measured 72 h after the start of treatment.RESULTS:The neurological function scores of the three Shuigou groups were significantly higher than those of the model group(P<0.05).There was no significant difference between sides in the latency and amplitude of MEPs in the model group(P> 0.05).The latency on the affected side in the model group was significantly longer than that in the control group(P<0.05) and the amplitude on affected side was significantly reduced(P<0.01).After 3 days of electro-acupuncture,the latency on the affected side in the 2 Hz Shuigou group was significantly shortened(P<0.05) and the amplitude was significantly increased(P<0.05) compared with the model group.CONCLUSION:Low frequency electro-acupuncture at Shuigou(GV 26) can promote recovery of motor function after focal cerebral ischemic injury in rats.

Acupuncture for the treatment of diminished ovary reserve

Objective To share 20 cases of women with diminished ovary reserve（DOR） and low Anti-Müllerian Hormone（AMH）, who had unsuccessful in-vitro fertilization（IVF） trials and to whom the author has successfully treated. Methods Twenty women with DOR had gone through the failed IVF from 1 to 5 times. The acupuncture protocol consists of electroacupuncture, manual acupuncture, acupressure and sliding-cupping, which are the multiple interventions. The acupoints were used based on 5 phases of the menstrual cycle. The electroacupuncture was used twice a week in the follicle phase and the acupuncture without the electricity was used once a week in the luteal phase. Most of them had been treated for three menstrual cycles. Results All of 20 women got pregnant by IVF（17 casese） or naturally（3 cases）. Conclusion Acupuncture might improve IVF outcomes for women with DOR by acupuncture treatment for three months or help them to receive naturally.

Electroacupuncture for functional dyspepsia and the influence on serum Ghrelin, CGRP and GLP-1 levels

Objective： To observe the clinical efficacy of electroacupuncture for functional dyspepsia（FD）, and explore the corresponding mechanism.Methods： Sixty-four FD patients were randomly divided into electroacupuncture group and western medicine group, with 32 cases in each group. In electroacupuncture group, electroacupuncture at Zusanli（足三里ST 36）,Sanyinjiao（三阴交SP 6）,Gongsun（公孙SP 4） and Neiguan（内关PC 6） was performed for once a day, and the needles were retained for 30 min. In western medicine group, oral administration of mosapride citrate dispersible tablets in a dosage of 5 mg/time was carried out for 3 times a day. Treatment was conducted for 30 consecutive days in both groups. The scores of Leeds dyspepsia questionnaire（LDQ） and functional digestive disorder quality of life（FDDQL） of patients in both groups were recorded before and after treatment. Serum Ghrelin, CGRP and GLP-1 levels of patients were tested before and after treatment respectively, and the clinical efficacy of patients in both groups was evaluated after treatment.Results： In western medicine group, LDQ score after treatment was lower than that before treatment（P 0.05）, FDDQL score after treatment was higher than that before treatment, while the differences were not statistically significant（P0.05）. LDQ score in electroacupuncture group after treatment was lower than that before treatment（P0.05）, and also lower than that in western medicine group at the same time point（P 0.05）. FDDQL score in electroacupuncture group after treatment was higher than that before treatment（P0.05）, and also higher than that in western medicine group at the same time point（P0.05）. In western medicine group, Ghrelin level after treatment was higher than that before treatment（P 0.05）, CGRP level reduced, and the differences were not statistically significant（P 0.05）. GLP-1 level after treatment was also higher than that before treatment（P0.05）. In electroacupuncture group,Ghrelin level after treatment was higher than that before treatment, CGRP level reduced, and GLP-1 level after treatment was also higher than that before treatment（both P 0.05）. According to the comparison of values of each index between electroacupuncture group and western medicine group after treatment,the differences were all statistically significant（all P 0.05）. The total effective rate in electroacupuncture group was 90.63%（29/32） which was higher than that in western medicine group 68.75%（22/32）, and the differences were statistically significant（P0.05）.Conclusion： Electroacupuncture at ST 36, SP 6, SP 4 and PC 6 can effectively improve the clinical symptoms of FD patients, and the mechanism might be related with the increase of serum Ghrelin and GLP-1 levels and the decrease of serum CGRP level.

Effect of electroacupuncture at the Pericardium Meridian on the heart function of volunteers with acute hypoxia

Objective： To study the effect of electroacupuncture （EA） at the Pericardium Meridian on the heart function of volunteers with acute hypoxia, and to provide scientific evidence for the acupoints selection along the affected meridian in acupuncture-moxibustion therapy. Methods： Based on a self-control design, eighteen healthy volunteers were recruited in the study. Points from the Pericardium Meridian [Neiguan （PC 6）, Ximen （PC 4）, Quze （PC 3） and Tianquan （PC 2）], non-Pericardium Meridian point [Shousanli （LI 10）], non-meridian and non-acupoint points [1.0-1.5 cm lateral to Neiguan （PC 6） and Ximen （PC 4）, respectively on both sides], and a blank control （only inhaling low-oxygen gas without EA stimulation] were selected to observe, once every week, 10 sessions in total, and only 1 acupoint was observed once. The volunteers inhaled low-oxygen gas mixture （10.8% 02 and 89.2% N2] for 30 min to imitate acute hypoxia. EA was conducted when the gas mixture was inhaled for 10 min and then lasted for 20 min; meanwhile, hemodynamic indexes such as cardiac output （CO）, cardiac index （Cl）, systemic vascular resistance （SVR）, systemic vascular resistance index （SVRI], left cardiac work （LCW], left cardiac work index （LCWl） and heart rate （HR） were recorded on a hemodynamic monitor. Results： EA at the acupoints of Pericardium Meridian significantly down-regulated the increased CO/CI, LCW/LCWl, and HR （P〈0.05）, and significantly up-regulated the decreased SVR/SVRI in hypoxia （P〈0.05）; EA at other meridian acupoints or at non-meridian and non-acupoint points didn＇t produce such effects. Conclusion： EA at the Pericardium Meridian can obviously improve the cardiac hyper-activation caused by acute hypoxia in healthy volunteers.