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中国药典抗生素微生物检定上层培养基中加菌量的研究 被引量:4
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作者 胡功允 张琪霞 《药物分析杂志》 CAS CSCD 北大核心 1997年第5期332-335,共4页
首次对中国药典抗生素微生物检定法中试验菌为藤黄八叠球菌、枯草芽孢杆菌、短小芽孢杆菌所检定抗生素品种的上层培养基中的加菌量进行了较为系统的研究。实验表明以规定原菌液的透光率和稀释数即可直接获得符合药典规定的抑菌圈大小,... 首次对中国药典抗生素微生物检定法中试验菌为藤黄八叠球菌、枯草芽孢杆菌、短小芽孢杆菌所检定抗生素品种的上层培养基中的加菌量进行了较为系统的研究。实验表明以规定原菌液的透光率和稀释数即可直接获得符合药典规定的抑菌圈大小,方法简便可靠、易于规范化统一操作。 展开更多
关键词 抗生素 微生物检定法 加菌量
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胞苷酸生产废水特种微生物筛选及处理效果研究
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作者 张飞龙 张玮琪 +1 位作者 梁国斌 赵阳 《中国科技纵横》 2024年第4期33-35,67,共4页
胞苷酸生产废水具有酸性强、有机物含量高、生物降解难等特点。本试验从胞苷酸生产废水中原位筛选一株特种微生物,经鉴定为绿脓杆菌(Pseudomonas sp.),研究其最佳生长条件。结果表明,绿脓杆菌最适宜的生长条件是pH值为7、生长温度为30... 胞苷酸生产废水具有酸性强、有机物含量高、生物降解难等特点。本试验从胞苷酸生产废水中原位筛选一株特种微生物,经鉴定为绿脓杆菌(Pseudomonas sp.),研究其最佳生长条件。结果表明,绿脓杆菌最适宜的生长条件是pH值为7、生长温度为30℃、振荡培养箱转速为0r/min。在最佳生长条件下探讨菌株投加量对胞苷酸生产废水的影响,绿脓杆菌菌液投加量为100%(v/v)时,反应72h,化学需氧量(ChemicalOxygenDemand,COD)降解率最高,为45.3%。 展开更多
关键词 胞苷酸生产废水 绿脓杆 种生长曲线 液投 生物降解
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特种微生物处理叶酸生产废水条件优化
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作者 张飞龙 张玮琪 +1 位作者 梁国斌 杜秦艺 《中国科技纵横》 2024年第1期56-58,共3页
工业生产叶酸不仅需要耗费大量的水,且在生产过程中也会产生大量有机物浓度高、盐浓度高、色度高、可生化性很差的废水。嗜酸耐盐特种微生物对叶酸生产废水的生物降解有重要影响。本实验从叶酸生产废水中筛选出特种微生物,经鉴定为大观... 工业生产叶酸不仅需要耗费大量的水,且在生产过程中也会产生大量有机物浓度高、盐浓度高、色度高、可生化性很差的废水。嗜酸耐盐特种微生物对叶酸生产废水的生物降解有重要影响。本实验从叶酸生产废水中筛选出特种微生物,经鉴定为大观贝氏菌(Byssochlamys spectabilis),绘制其生长曲线,考察特种微生物菌液投加量对废水的降解效果。结果表明,大观贝氏菌菌液投加量为50%(v/v)时,反应4天后,废水化学需氧量(Chemical oxygen demand,COD)和苯胺降解率均达最高,分别为84.90%和83.26%。 展开更多
关键词 叶酸生产废水 大观贝氏 种生长曲线 液投 生物降解
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庆大霉素效价测定方法的改进
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作者 徐建民 吕凤萍 《菏泽医学专科学校学报》 1997年第2期39-40,共2页
关键词 效价测定方法 氨基糖甙类抗生素 生物效价测定 庆大霉素C 琼脂培养基 中国药典 化合物 多组分 高剂 加菌量 缓冲液
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Fermented Goat Milk and Cow Milk Produced by Different Starters of Lactic Acid Bacteria: Quality Studies 被引量:1
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作者 Widodo Tiyas Tono Taufiq Nosa Septiana Anindita 《Journal of Agricultural Science and Technology(A)》 2013年第11期904-911,共8页
Lactic acid bacteria (LAB) is widely used as culture starters in dairy fermentation. The aim of this study was to investigate the quality of fermented goat milk and cow milk, as well as the viability of LAB in the s... Lactic acid bacteria (LAB) is widely used as culture starters in dairy fermentation. The aim of this study was to investigate the quality of fermented goat milk and cow milk, as well as the viability of LAB in the same products. Fermentations were performed with pasteurized goat milk or cow milk added with skim milk (18% of solids) using three separately different starters; yoghurt starter (a combination of Streptococcus thermophilus FNCC-0040 and Lactobacillus bulgaricus FNCC-0041), single starter of Lactobacillus acidophilus FNCC-0029 and Lactobacillus casei FNCC-0051. The parameters observed were pH, acidity, nutritional quality including protein, fat and lactose content and product's viscosity. Acidity, pH and viability of LAB were also monitored during storage at refrigerated temperature (4 ℃) for 28 days. Results show that the different LAB starters did not affect the pH, acidity, lactose and protein content. Differences on LAB starters affected fat content and viscosity. The highest score of viscosity (30.00 Pa.s ± 7.02 Pa.s) was observed on products fermented by yoghurt starters, followed by products obtained using starter of L. acidophilus (17.7 ±11.4) and L. casei (8.62 ±0.35). Protein content, acidity, pH and viscosity were not significantly different between products obtained from goat milk and cow milk. Fat content in fermented goat milk was higher (5.03% ±0.62%) than in fermented cow milk (3.52% ±0.37%), however, lactose content was higher in fermented cow milk (5.16% ±0.40%) than in fermented goat milk (4.53% ±0.35%). Total LAB concentration in fermented cow milk during storage was 8.03± 0.52 logt0 cfu/mL, while in fermented goat milk was 7.81 loglo cfu/mL ± 0.67 loglo cfu/mL. There was a 10.83% decrease in LAB viability in fermented cow milk and 11.40% in fermented goat milk after 28 days of storage. In conclusion, quality of fermented milk is affected by the starters applied, raw milk source and storage period. 展开更多
关键词 Lactic acid bacteria fermented milk goat milk cow milk viability.
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The Content of Selenium and Other Ten Chemical Elements in Boletus Edulis from Bulgaria and Inhibition of Lipid Peroxidation
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作者 G. Bekyarov M. Kakalova +6 位作者 A. Iliev Tsv. Bekyarova G.V. Donchenko O.M. Palyvoda O.V. Vydiuk A.S.Dubovetskyi O.I. Kuzmenko 《Journal of Environmental Science and Engineering》 2011年第6期708-715,共8页
The goal of this study was to determine the content of such biological active metals as Se, Hg, AI, Cu, Zn, As, Cd, Pb, Mg, Ca and Fe in Boletus Edulis mushrooms and to study the effect of mushrooms as inhibitors of b... The goal of this study was to determine the content of such biological active metals as Se, Hg, AI, Cu, Zn, As, Cd, Pb, Mg, Ca and Fe in Boletus Edulis mushrooms and to study the effect of mushrooms as inhibitors of blood serum copper-initiated lipid peroxidation. The metals content was determined by ICP-OES technique and blood lipids peroxidation in vitro was assessed by thiobarbituric acid-reactive substances measurement. The dependency between quality and content of the determined biological active metals has been traced. Samples were analyzed of wild growing mushrooms Boletus Edulis from two mountain regions in Bulgaria. On the average the content of Se in Boletus Edulis was found to be 25 mg/kg dried mushroom, this content being higher in tubules than in fleshy part. We found that Boletus Edulis mushrooms inhibited lipid peroxidation in the concentration dependent manner. The effective concentration of Boletus Edulis is in 5 times lower compared to the concentration of Cantharellus Cibarius resulting in similar lipid peroxidation inhibition. This effect can be explained by 56 times higher content of Se and by 1.5 and 3 times lower content of such initiators of lipid peroxidation as Cu and Fe in Boletus Edulis compared to Cantharellus Cibarius. A system with a source of infrared radiation heating, developed by authors, was used for the mushroom mineralization. We conclude that Boletus Edulis is an effective inhibitor of blood lipid peroxidation and in 5 times stronger rather than Cantharellus Cibarius. 展开更多
关键词 Boletus edulis ICP-OES heavy metals SELENIUM lipid peroxidation ANTIOXIDANTS
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