Surface modification of medical implants was considered as an effective method to improve the cellular behaviors and the integration of tissue onto materials. The micro-nanostructured surface on the titanium alloy was...Surface modification of medical implants was considered as an effective method to improve the cellular behaviors and the integration of tissue onto materials. The micro-nanostructured surface on the titanium alloy was prepared by laser treatment and multiple acid etching. The surface morphologies of different titanium alloy substrates were characterized by scanning electron microscopy (SEM). The effects of micro-nanostructured surfaces on the cellular responses were investigated in vitro by observing hydroxyapatite formation, cell morphology and cell adhesion. The results indicate that the micro-sized structure promoted the adhesion and proliferation of cultured osteoblasts. Furthermore, the micro-nanostructured surface was more conducive to cell adhension stretching compared with the micro-structured surface. All results suggest that the micro-nanostructured surface improved the biocompatibility and integration of tissue onto titanium alloy implants.展开更多
In an effort to simplify the procedure and to reduce the cost of fluorescence SSR analysis, the conditions of the multiplex PCR and the multiplex gel electrophoresis were optimized in the genetic analysis of sunflower...In an effort to simplify the procedure and to reduce the cost of fluorescence SSR analysis, the conditions of the multiplex PCR and the multiplex gel electrophoresis were optimized in the genetic analysis of sunflower (Helianthus annuus L.) inbred lines. Results indicated that factors for a successful multiplex PCR assay were related to the cycling touchdown annealing temperature, the balance of primer concentration at the various loci, the concentration of PCR buffer and the Taq DNA polymerase. Based on the optimization, a tailed primer strategy was outlined, and the effective ways were proposed to overcome the troubleshootings commonly encountered in the multiplex PCR and the multiplex gel electrophoresis.展开更多
The interplay between analytical technique and industrial practice has been central in the development of catalytic materials for processing petroleum. This article presents reviews of key aspects of two of the most i...The interplay between analytical technique and industrial practice has been central in the development of catalytic materials for processing petroleum. This article presents reviews of key aspects of two of the most important classes of catalytic materials: noble-metal Pt nanoparticles (NPs) on alumina, which are the basis of catalytic reforming;and layered sulfides of Mo and W, which catalyze hydrogenation and hetero-atom removal in hydroprocessing. The state of understanding of Pt cluster growth and resulting structures, as developed using X-ray absorption spectroscopy and STEM, is reviewed. Influences of both Pt reduction temperature in hydrogen gas, and oxidizing pretreatment conditions prior to Pt reduction, are considered. Recent work by the present authors on Pt NP structure evolution is presented in the context of the previous work. A review is subsequently presented of layered sulfide based NPs, summarizing contributions from a range of analytical techniques. Work on active site structures of sulfide NPs is reviewed, focusing particularly on the critical interactions of active edge sites with sulfur and hydrogen in chemisorption, physisorption, and spillover interactions. New temperature programmed reduction (TPR) results are presented for supported and unsupported sulfide NPs. Structural changes in TPR of alumina-supported MoS2 are investigated using extended X-ray absorption fine structure and density functional theory modeling, and are determined to arise from removal of identifiable edge-site sulfur species.展开更多
Present-day conditions of the Lake Kenon ecosystem are determined by a combination of natural and anthropogenic factors. We have estimated the effects of a complex of factors on the condition of the abiotic environmen...Present-day conditions of the Lake Kenon ecosystem are determined by a combination of natural and anthropogenic factors. We have estimated the effects of a complex of factors on the condition of the abiotic environment and on specific biological components in the lake ecosystem. Change in biogenic load has caused an increase in the role of phytoplankton in the general balance of organic matter during the high-water period. Charophytes are the main dominants of bottom vegetation. Anthropogenic load has caused a decrease in both fish species and fish capacity. The lake application as a water reservoir-cooler has influenced the average annual water mineralization （from 420 mg/L to 530.0 mg/L with a maximum 654 mg/L in 1993） and fluctuations in its hydrochemical composition. The present composition of the lake is sulfate-hydrocarbonate-chloride calcium-sodic-magnesium in character. S（Y44 content is twice as much as the maximum permissible concentration in fishery waters. Water drainage from an ash disposal area to the lake has caused an increase in chemical-element concentrations including the heavy metals. Hg concentration in Perca fluviatilis muscles is 0.5 9g/g dry wt. Thus, understanding directions in the ecosystem of the water reservoir-cooler under changing hydrological conditions will let us forecast the consequences of new combined heat and power plant operation.展开更多
Atmospheric nitrogen （N） deposition caused by anthropogenic activities may alter litter decomposition and species composition, and then affect N cycling and carbon （C） sequestration in an ecosystem. Using the litt...Atmospheric nitrogen （N） deposition caused by anthropogenic activities may alter litter decomposition and species composition, and then affect N cycling and carbon （C） sequestration in an ecosystem. Using the litterbag method, we studied the effects of N addition （CK： no N addition; low-N： 1 g N m-2 y-l; high-N： 2 g N m-2 y-l） on changes in mass remaining of shoot litter decomposition of three grasses （Stipa baicalensis, Carex pediformis and Leymus chinensis） over 28 months in the Hulun Buir meadow steppe of Inner Mongolia. The results showed that the addition of high and low N had no significant effect on the decomposition of single-species litter, but low N addition slightly inhibited the decomposition of litter mixtures. In addition, litter decomposition was strongly species dependent. Our results suggest that species type is likely the main determinant of litter decomposition, and low N deposition in natural ecosystems does not influence single-species litter decomposition.展开更多
Perovskite crystal film quality is critical for obtaining efficient perovskite solar cells. Anti-solvent processing was used for fast crystallization of perovskite precursor film, which can form dense perovskite film....Perovskite crystal film quality is critical for obtaining efficient perovskite solar cells. Anti-solvent processing was used for fast crystallization of perovskite precursor film, which can form dense perovskite film. However, the crystals from this method are usually small due to the fast crystal growth process, which could lead to grain boundary recombination. Here, element chloride is introduced to enhance the perovskite layer crystallinity via slowing down the perovskite crystallization process by simultaneous introduction of methylammounium chloride （MACI） and cesium chloride （CsCl） into precursor solution. As a result, we achieve high quality of pin-hole free perovskite film with large crystal size. A power conversion efficiency of 21.55% with free of hysteresis of the device is obtained, which is among the highest efficiency of planar structure perovskite solar cells.展开更多
基金Projects(5117530651575320)supported by the National Natural Science Foundation of China+1 种基金Project(TS20130922)supported by the Taishan Scholar Foundation,ChinaProject(2014JC020)supported by the Fundamental Research Funds for the Central Universities of China
文摘Surface modification of medical implants was considered as an effective method to improve the cellular behaviors and the integration of tissue onto materials. The micro-nanostructured surface on the titanium alloy was prepared by laser treatment and multiple acid etching. The surface morphologies of different titanium alloy substrates were characterized by scanning electron microscopy (SEM). The effects of micro-nanostructured surfaces on the cellular responses were investigated in vitro by observing hydroxyapatite formation, cell morphology and cell adhesion. The results indicate that the micro-sized structure promoted the adhesion and proliferation of cultured osteoblasts. Furthermore, the micro-nanostructured surface was more conducive to cell adhension stretching compared with the micro-structured surface. All results suggest that the micro-nanostructured surface improved the biocompatibility and integration of tissue onto titanium alloy implants.
文摘In an effort to simplify the procedure and to reduce the cost of fluorescence SSR analysis, the conditions of the multiplex PCR and the multiplex gel electrophoresis were optimized in the genetic analysis of sunflower (Helianthus annuus L.) inbred lines. Results indicated that factors for a successful multiplex PCR assay were related to the cycling touchdown annealing temperature, the balance of primer concentration at the various loci, the concentration of PCR buffer and the Taq DNA polymerase. Based on the optimization, a tailed primer strategy was outlined, and the effective ways were proposed to overcome the troubleshootings commonly encountered in the multiplex PCR and the multiplex gel electrophoresis.
基金the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357
文摘The interplay between analytical technique and industrial practice has been central in the development of catalytic materials for processing petroleum. This article presents reviews of key aspects of two of the most important classes of catalytic materials: noble-metal Pt nanoparticles (NPs) on alumina, which are the basis of catalytic reforming;and layered sulfides of Mo and W, which catalyze hydrogenation and hetero-atom removal in hydroprocessing. The state of understanding of Pt cluster growth and resulting structures, as developed using X-ray absorption spectroscopy and STEM, is reviewed. Influences of both Pt reduction temperature in hydrogen gas, and oxidizing pretreatment conditions prior to Pt reduction, are considered. Recent work by the present authors on Pt NP structure evolution is presented in the context of the previous work. A review is subsequently presented of layered sulfide based NPs, summarizing contributions from a range of analytical techniques. Work on active site structures of sulfide NPs is reviewed, focusing particularly on the critical interactions of active edge sites with sulfur and hydrogen in chemisorption, physisorption, and spillover interactions. New temperature programmed reduction (TPR) results are presented for supported and unsupported sulfide NPs. Structural changes in TPR of alumina-supported MoS2 are investigated using extended X-ray absorption fine structure and density functional theory modeling, and are determined to arise from removal of identifiable edge-site sulfur species.
基金Supported by the RFBR No.14-05-98013"Siberia"(2014–2016)the Project of SB of the RAS VIII.79.1.2."Dynamics of natural and natural-anthropogenic systems in the conditions of climate change and anthropogenic pressures(on the example of Transbaikalia)"(2012–2017)
文摘Present-day conditions of the Lake Kenon ecosystem are determined by a combination of natural and anthropogenic factors. We have estimated the effects of a complex of factors on the condition of the abiotic environment and on specific biological components in the lake ecosystem. Change in biogenic load has caused an increase in the role of phytoplankton in the general balance of organic matter during the high-water period. Charophytes are the main dominants of bottom vegetation. Anthropogenic load has caused a decrease in both fish species and fish capacity. The lake application as a water reservoir-cooler has influenced the average annual water mineralization （from 420 mg/L to 530.0 mg/L with a maximum 654 mg/L in 1993） and fluctuations in its hydrochemical composition. The present composition of the lake is sulfate-hydrocarbonate-chloride calcium-sodic-magnesium in character. S（Y44 content is twice as much as the maximum permissible concentration in fishery waters. Water drainage from an ash disposal area to the lake has caused an increase in chemical-element concentrations including the heavy metals. Hg concentration in Perca fluviatilis muscles is 0.5 9g/g dry wt. Thus, understanding directions in the ecosystem of the water reservoir-cooler under changing hydrological conditions will let us forecast the consequences of new combined heat and power plant operation.
基金the National Basic Research Program of China (2010CB833501 973 Program)National Major Research Program of China about climate change (2010CB950603)
文摘Atmospheric nitrogen （N） deposition caused by anthropogenic activities may alter litter decomposition and species composition, and then affect N cycling and carbon （C） sequestration in an ecosystem. Using the litterbag method, we studied the effects of N addition （CK： no N addition; low-N： 1 g N m-2 y-l; high-N： 2 g N m-2 y-l） on changes in mass remaining of shoot litter decomposition of three grasses （Stipa baicalensis, Carex pediformis and Leymus chinensis） over 28 months in the Hulun Buir meadow steppe of Inner Mongolia. The results showed that the addition of high and low N had no significant effect on the decomposition of single-species litter, but low N addition slightly inhibited the decomposition of litter mixtures. In addition, litter decomposition was strongly species dependent. Our results suggest that species type is likely the main determinant of litter decomposition, and low N deposition in natural ecosystems does not influence single-species litter decomposition.
基金supported by National 1000 Young Talents AwardsNational Key Research and Development Program of China(2016YFB0700700)+1 种基金National Natural Science Foundation of China(61634001,61574133)supported by Hanergy Group
文摘Perovskite crystal film quality is critical for obtaining efficient perovskite solar cells. Anti-solvent processing was used for fast crystallization of perovskite precursor film, which can form dense perovskite film. However, the crystals from this method are usually small due to the fast crystal growth process, which could lead to grain boundary recombination. Here, element chloride is introduced to enhance the perovskite layer crystallinity via slowing down the perovskite crystallization process by simultaneous introduction of methylammounium chloride （MACI） and cesium chloride （CsCl） into precursor solution. As a result, we achieve high quality of pin-hole free perovskite film with large crystal size. A power conversion efficiency of 21.55% with free of hysteresis of the device is obtained, which is among the highest efficiency of planar structure perovskite solar cells.
