目的:探讨大鼠睾丸组织COXⅦa(cytochrome C oxidase Ⅶ a)表达的加龄变化及其与睾酮合成之间的相关性。方法:①选用出生后1周、3周、5周、3月和24月龄雄性SD大鼠,每组6只。②采用半定量RT-PCR法检测大鼠睾丸组织COXⅦamRNA的表达。③We...目的:探讨大鼠睾丸组织COXⅦa(cytochrome C oxidase Ⅶ a)表达的加龄变化及其与睾酮合成之间的相关性。方法:①选用出生后1周、3周、5周、3月和24月龄雄性SD大鼠,每组6只。②采用半定量RT-PCR法检测大鼠睾丸组织COXⅦamRNA的表达。③Western blotting方法检测大鼠睾丸组织COXⅦa蛋白表达。④化学发光法检测大鼠血清睾酮浓度。结果:①血清睾酮浓度3月、24月、5周、3周和1周龄组依次降低,其中3月龄和24月龄组与其余各组间差异有统计学意义(P<0.05)。②COXⅦamRNA水平24月、1周、3周、5周和3月龄组依次降低,其中3月龄和24月龄组与其余各组之间差异有统计学意义(P<0.05)。③睾丸组织COXⅦa蛋白表达,24月龄组最强,3月龄组最弱,除1周龄和3周龄组之间差异无统计学意义外,其余各组之间差异均有统计学意义(P<0.05)。④睾酮浓度与COXⅦamRNA水平和蛋白表达呈负相关(r=-0.580,P=0.013;r=-0.447,P=0.000),而COXⅦamRNA水平和蛋白表达呈正相关(r=0.941,P=0.000)。结论:COXⅦa随增龄变化,老年大鼠COXⅦa显著上调,与睾酮合成能力下降有关,可能成为治疗PADAM的候选基因。展开更多
[ Objective] The aim of this study was to provide a theoretical basis for population and application of Chinese herbal feed additive in the rex rabbit breeding. [ Methyl] 108 weaned -rex rabbits aged 40 days were divi...[ Objective] The aim of this study was to provide a theoretical basis for population and application of Chinese herbal feed additive in the rex rabbit breeding. [ Methyl] 108 weaned -rex rabbits aged 40 days were divided into three groups with thirty -six each group. Group A were fed basal diet, while group B were fed basal diet supplemented with 0.3% Chinese herbal feed additive and group C were fed basal diet supplemented with 0.4% Chinese herbal feed additive. The meat quality indexes were measured when feeding to 150 age in days. [ Result] There was significant difference among pH value, water loss rate, storing loss rate and crude protein content in each group （P〈0.05）, but no significant difference was found in tenderness, cooking percentage, moisture content and crude fat content （ P 〉 0.05）. The total content of ami- no acids, essential amino acids and semi essential amino acids as well as main delicious amino acids was highest in group C accounting for 23.84%, 11.47% and 8.32% respectively, next came group B accounting for 20.94%, 10.64% and 6.81% respectively, and lowest in group C （ control group） accounting for 19.06%, 9.61% and 6.46% respectively. [ Conclusion] The Chinese herbal feed additive can improve meat quality of rex rabbit the best addition of 0.4%.展开更多
文摘[ Objective] The aim of this study was to provide a theoretical basis for population and application of Chinese herbal feed additive in the rex rabbit breeding. [ Methyl] 108 weaned -rex rabbits aged 40 days were divided into three groups with thirty -six each group. Group A were fed basal diet, while group B were fed basal diet supplemented with 0.3% Chinese herbal feed additive and group C were fed basal diet supplemented with 0.4% Chinese herbal feed additive. The meat quality indexes were measured when feeding to 150 age in days. [ Result] There was significant difference among pH value, water loss rate, storing loss rate and crude protein content in each group （P〈0.05）, but no significant difference was found in tenderness, cooking percentage, moisture content and crude fat content （ P 〉 0.05）. The total content of ami- no acids, essential amino acids and semi essential amino acids as well as main delicious amino acids was highest in group C accounting for 23.84%, 11.47% and 8.32% respectively, next came group B accounting for 20.94%, 10.64% and 6.81% respectively, and lowest in group C （ control group） accounting for 19.06%, 9.61% and 6.46% respectively. [ Conclusion] The Chinese herbal feed additive can improve meat quality of rex rabbit the best addition of 0.4%.
