In this study,circular dichroism(CD)and molecular dynamics(MD)simulation were used to investigate the thermal unfolding pathway of staphylococcal enterotoxin B(SEB)at temperatures of 298–371 and 298–500 K,and the re...In this study,circular dichroism(CD)and molecular dynamics(MD)simulation were used to investigate the thermal unfolding pathway of staphylococcal enterotoxin B(SEB)at temperatures of 298–371 and 298–500 K,and the relationship between the experimental and simulation results were explored.Our computational findings on the secondary structure of SEB showed that at room temperature,the CD spectroscopic results were highly consistent with the MD results.Moreover,under heating conditions,the changing trends of helix,sheet and random coil obtained by CD spectral fitting were highly consistent with those obtained by MD.In order to gain a deeper understanding of the thermal stability mechanism of SEB,the MD trajectories were analyzed in terms of root mean square deviation(RMSD),secondary structure assignment(SSA),radius of gyration(R_(g)),free energy surfaces(FES),solvent-accessible surface area(SASA),hydrogen bonds and salt bridges.The results showed that at low heating temperature,domain Ⅰ without loops(omitting the mobile loop region)mainly relied on hydrophobic interaction to maintain its thermal stability,whereas the thermal stability of domain Ⅱ was mainly controlled by salt bridges and hydrogen bonds.Under high heating temperature conditions,the hydrophobic interactions in domain Ⅰ without loops were destroyed and the secondary structure was almost completely lost,while domain Ⅱ could still rely on salt bridges as molecular staples to barely maintain the stability of the secondary structure.These results help us to understand the thermodynamic and kinetic mechanisms that maintain the thermal stability of SEB at the molecular level,and provide a direction for establishing safer and more effective food sterilization processes.展开更多
The now and heat transfer characteristics in tenon joint gap between turbine blade and disk have been investigated experimentally with a scale up model. The characteristics of flow and heat transfer in this speCial ga...The now and heat transfer characteristics in tenon joint gap between turbine blade and disk have been investigated experimentally with a scale up model. The characteristics of flow and heat transfer in this speCial gap passage have been analyzed. The results are useful for beat transfer analysis in turbine design.展开更多
In this paper, we study a class singular perturbed elliptic equation boundary value problem with a super surface of turning point in n-dimensional space by using the method of multiple scales and the comparison theore...In this paper, we study a class singular perturbed elliptic equation boundary value problem with a super surface of turning point in n-dimensional space by using the method of multiple scales and the comparison theorem. The uniformly valid asymptotic approxmations of solutions for the boundary value problem is constructed.展开更多
The forming of elliptic motions on the modal conversion ultrasonic motors （MCUMs） is discussed. The principles of the modal conversion are investigated based on the coupling with the stator and the rotor, and using ...The forming of elliptic motions on the modal conversion ultrasonic motors （MCUMs） is discussed. The principles of the modal conversion are investigated based on the coupling with the stator and the rotor, and using an independent coupler. The elliptical locus observed on the longitudinal-torslonal vibration converter with oblique slits is analyzed by using vibration theory. A method for the modal conversion is proposed by using the local mode of a substructure On a main structure. The method can be used to design the modal conversion type ultrasonic motors.展开更多
The hydrodynamic characteristics of a rigid, single, circular cylinder in a three dimensional, incompressible, uniform cross flow were calculated using the large-eddy simulation method of CFX5. Solutions to the three ...The hydrodynamic characteristics of a rigid, single, circular cylinder in a three dimensional, incompressible, uniform cross flow were calculated using the large-eddy simulation method of CFX5. Solutions to the three dimensional N-S equations were obtained by the finite volume method. The focus of this numerical simulation was to research the characteristics of pressure distribution （drag and litt forces） and vortex tubes at high Reynolds numbers. The results of the calculations showed that the forces at every section in the spanwise direction of the cylinder were symmetrical about the middle section and smaller than the forces calculated in two dimensional cases. Moreover, the flow around the cylinder obviously presents three dimensional characteristics.展开更多
Aiming to improve the maneuver performance of the strapdown inertial navigation attitude coning algorithm a new coning correction structure is constructed by adding a sample to the traditional compressed coning correc...Aiming to improve the maneuver performance of the strapdown inertial navigation attitude coning algorithm a new coning correction structure is constructed by adding a sample to the traditional compressed coning correction structure. According to the given definition of classical coning motion the residual coning correction error based on the new coning correction structure is derived. On the basis of the new structure the frequency Taylor series method is used for designing a coning correction structure coefficient and then a new coning algorithm is obtained.Two types of error models are defined for the coning algorithm performance evaluation under coning environments and maneuver environments respectively.Simulation results indicate that the maneuver accuracy of the new 4-sample coning algorithm is almost double that of the traditional compressed 4-sample coning algorithm. The new coning algorithm has an improved maneuver performance while maintaining coning performance compared to the traditional compressed coning algorithm.展开更多
The singularly perturbed elliptic equation boundary value problem with turning point is considered. Using the method of multiple scales and the comparison theorem, the asymptotic behavior of solution for the boundary ...The singularly perturbed elliptic equation boundary value problem with turning point is considered. Using the method of multiple scales and the comparison theorem, the asymptotic behavior of solution for the boundary value problem is studied.展开更多
The relation between the normal displacement on the surface of a dynamical elliptical crack and the normal stress over the crack surface was studied. The three dimensional elastodynamic equations and Fourier Laplace...The relation between the normal displacement on the surface of a dynamical elliptical crack and the normal stress over the crack surface was studied. The three dimensional elastodynamic equations and Fourier Laplace transforms are used. Based on the influence function and the inversion of integral transforms, one can find that if the distribution of normal displacement on the surface of a dynamic elliptical crack is a polynomial of degree n in x 1 and x 2 , then the normal pressure acting over the ellipse is also a polynomial P n(x 1,x 2) of the same degree in x 1 and x 2 .展开更多
文摘In this study,circular dichroism(CD)and molecular dynamics(MD)simulation were used to investigate the thermal unfolding pathway of staphylococcal enterotoxin B(SEB)at temperatures of 298–371 and 298–500 K,and the relationship between the experimental and simulation results were explored.Our computational findings on the secondary structure of SEB showed that at room temperature,the CD spectroscopic results were highly consistent with the MD results.Moreover,under heating conditions,the changing trends of helix,sheet and random coil obtained by CD spectral fitting were highly consistent with those obtained by MD.In order to gain a deeper understanding of the thermal stability mechanism of SEB,the MD trajectories were analyzed in terms of root mean square deviation(RMSD),secondary structure assignment(SSA),radius of gyration(R_(g)),free energy surfaces(FES),solvent-accessible surface area(SASA),hydrogen bonds and salt bridges.The results showed that at low heating temperature,domain Ⅰ without loops(omitting the mobile loop region)mainly relied on hydrophobic interaction to maintain its thermal stability,whereas the thermal stability of domain Ⅱ was mainly controlled by salt bridges and hydrogen bonds.Under high heating temperature conditions,the hydrophobic interactions in domain Ⅰ without loops were destroyed and the secondary structure was almost completely lost,while domain Ⅱ could still rely on salt bridges as molecular staples to barely maintain the stability of the secondary structure.These results help us to understand the thermodynamic and kinetic mechanisms that maintain the thermal stability of SEB at the molecular level,and provide a direction for establishing safer and more effective food sterilization processes.
文摘The now and heat transfer characteristics in tenon joint gap between turbine blade and disk have been investigated experimentally with a scale up model. The characteristics of flow and heat transfer in this speCial gap passage have been analyzed. The results are useful for beat transfer analysis in turbine design.
文摘In this paper, we study a class singular perturbed elliptic equation boundary value problem with a super surface of turning point in n-dimensional space by using the method of multiple scales and the comparison theorem. The uniformly valid asymptotic approxmations of solutions for the boundary value problem is constructed.
基金Supported by the National Natural Science Foundation of China(10874090,50775109)the Jiangsu Provincial High-Tech Project of China(BG2006005)~~
文摘The forming of elliptic motions on the modal conversion ultrasonic motors （MCUMs） is discussed. The principles of the modal conversion are investigated based on the coupling with the stator and the rotor, and using an independent coupler. The elliptical locus observed on the longitudinal-torslonal vibration converter with oblique slits is analyzed by using vibration theory. A method for the modal conversion is proposed by using the local mode of a substructure On a main structure. The method can be used to design the modal conversion type ultrasonic motors.
文摘The hydrodynamic characteristics of a rigid, single, circular cylinder in a three dimensional, incompressible, uniform cross flow were calculated using the large-eddy simulation method of CFX5. Solutions to the three dimensional N-S equations were obtained by the finite volume method. The focus of this numerical simulation was to research the characteristics of pressure distribution （drag and litt forces） and vortex tubes at high Reynolds numbers. The results of the calculations showed that the forces at every section in the spanwise direction of the cylinder were symmetrical about the middle section and smaller than the forces calculated in two dimensional cases. Moreover, the flow around the cylinder obviously presents three dimensional characteristics.
基金The National Natural Science Foundation of China(No.51375087)the Specialized Research Fund for the Doctoral Program of Higher Education(No.20110092110039)+2 种基金the Public Science and Technology Research Funds Projects of Ocean(No.201205035)the Scientific Innovation Research of College Graduates in Jiangsu Province(No.CXZZ12_0097)the Scientific Research Foundation of Graduate School of Southeast University(No.YBJJ1349)
文摘Aiming to improve the maneuver performance of the strapdown inertial navigation attitude coning algorithm a new coning correction structure is constructed by adding a sample to the traditional compressed coning correction structure. According to the given definition of classical coning motion the residual coning correction error based on the new coning correction structure is derived. On the basis of the new structure the frequency Taylor series method is used for designing a coning correction structure coefficient and then a new coning algorithm is obtained.Two types of error models are defined for the coning algorithm performance evaluation under coning environments and maneuver environments respectively.Simulation results indicate that the maneuver accuracy of the new 4-sample coning algorithm is almost double that of the traditional compressed 4-sample coning algorithm. The new coning algorithm has an improved maneuver performance while maintaining coning performance compared to the traditional compressed coning algorithm.
文摘The singularly perturbed elliptic equation boundary value problem with turning point is considered. Using the method of multiple scales and the comparison theorem, the asymptotic behavior of solution for the boundary value problem is studied.
文摘The relation between the normal displacement on the surface of a dynamical elliptical crack and the normal stress over the crack surface was studied. The three dimensional elastodynamic equations and Fourier Laplace transforms are used. Based on the influence function and the inversion of integral transforms, one can find that if the distribution of normal displacement on the surface of a dynamic elliptical crack is a polynomial of degree n in x 1 and x 2 , then the normal pressure acting over the ellipse is also a polynomial P n(x 1,x 2) of the same degree in x 1 and x 2 .
