The Preston＇s method is considered as one of the most commonly employed methods to measure the wall shear stress. However, it is only possible to determine the wall shear stress from measured pressure differences of ...The Preston＇s method is considered as one of the most commonly employed methods to measure the wall shear stress. However, it is only possible to determine the wall shear stress from measured pressure differences of the Preston tube and undisturbed static pressure, combined with calibration curves, which depend on the Preston tube diameter, fluid density, and viscosity. Since its invention, no significant advancement in theory has been made, and calibration curves proposed by Preston, Patel and Bechert are still in use. In the present study, a need to measure surface shear stress over a circular cylinder prompted us to develop our original Preston tube system. The developed system has been calibrated by measuring the wall shear stress in the fully developed turbulent flow regime in a circular pipe. The present results generally confirm the previously reported calibration curves. A slight modification of the coefficients in the calibration equation shows further improvement.展开更多
Objective To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and TRH receptor (TRH-R), and their role in the development of rat testis.Methods Oligonucleotide primers were designed from...Objective To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and TRH receptor (TRH-R), and their role in the development of rat testis.Methods Oligonucleotide primers were designed from the sequences of rat hypothalamus prepro TRH (ppTRH) and pituitary TRH-R cDNA for reverse transcription polymerase chain reaction (RT-PCR). Specific fragments of ppTRH and TRH-R cDNA were cloned and sequenced. Expression plasmids containing ppTRH and TRH-R genes were then constructed, and expression was found in E.coli DH5-α. ppTRH and TRH-R mRNA in the testis was quantitated in RNA samples prepared from rats at different developmental stages by real time quantitative RT-PCR.Results The quantitative analyses demonstrated that no ppTRH and TRH mRNA could be detected at the earliest stage (day 8). ppTRH and TRH mRNA signals were detected on day 15 and increased progressively on days 20, 35, 60 and 90. Conclusion Our results suggest that rat testis could specifically express TRH and TRH-R, and the transcriptions of ppTRH and TRH-R genes in the rat testis were development-dependent. The acquirement of expressed products for ppTRH and TRH-R can be used for further research on the physiological significance of TRH and TRH-R expression in rat testis.展开更多
文摘The Preston＇s method is considered as one of the most commonly employed methods to measure the wall shear stress. However, it is only possible to determine the wall shear stress from measured pressure differences of the Preston tube and undisturbed static pressure, combined with calibration curves, which depend on the Preston tube diameter, fluid density, and viscosity. Since its invention, no significant advancement in theory has been made, and calibration curves proposed by Preston, Patel and Bechert are still in use. In the present study, a need to measure surface shear stress over a circular cylinder prompted us to develop our original Preston tube system. The developed system has been calibrated by measuring the wall shear stress in the fully developed turbulent flow regime in a circular pipe. The present results generally confirm the previously reported calibration curves. A slight modification of the coefficients in the calibration equation shows further improvement.
文摘Objective To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and TRH receptor (TRH-R), and their role in the development of rat testis.Methods Oligonucleotide primers were designed from the sequences of rat hypothalamus prepro TRH (ppTRH) and pituitary TRH-R cDNA for reverse transcription polymerase chain reaction (RT-PCR). Specific fragments of ppTRH and TRH-R cDNA were cloned and sequenced. Expression plasmids containing ppTRH and TRH-R genes were then constructed, and expression was found in E.coli DH5-α. ppTRH and TRH-R mRNA in the testis was quantitated in RNA samples prepared from rats at different developmental stages by real time quantitative RT-PCR.Results The quantitative analyses demonstrated that no ppTRH and TRH mRNA could be detected at the earliest stage (day 8). ppTRH and TRH mRNA signals were detected on day 15 and increased progressively on days 20, 35, 60 and 90. Conclusion Our results suggest that rat testis could specifically express TRH and TRH-R, and the transcriptions of ppTRH and TRH-R genes in the rat testis were development-dependent. The acquirement of expressed products for ppTRH and TRH-R can be used for further research on the physiological significance of TRH and TRH-R expression in rat testis.
