Comparison and Analysis on Animal and Plant Quarantine of Entry Mails among China, Australia and New Zealand

With the significant increase of the number of entry mails in China, mail inspection has become more and more challenge. Australia and New Zealand are the two countries recognized as the ones with the most stringent inspection and quarantine measures in the world. The mail inspection work in China, Australia and New Zealand was compared on the aspects of mail quarantine system, inspection, quarantine treatment and punishment, based on which something worth learning and referring could be found and some suggestions were put forward, with the aim to provide theoretical basis for the animal and plant quarantine of entry mails.

Establishment of orthotopic transplantation model of human bladder cancer and detection by MRI

Objective: To establish an orthotopic bladder cancer model bearing human bladder cancer for experimental research, and monitor tumor progression by magnetic resonance imaging (MRI). Methods: The mucosa was mechanically damaged transurethrally under direct vision, and then human bladder cancer cell line T24 was inoculated into the bladders of BALB/c nude mice to establish orthotopic bladder cancer model. To find a suitable concentration of Gd-DTPA for this re- search. MRI was performed weekly to assess tumor growth, using Gd-DTPA as contrast agent. The pathologic morphology of the bladders and other specimens were observed with HE stain. Results: All the 25 mice developed bladder cancer after inoculation. The best concentration of Gd-DTPA was 1.408 mg/mL. On MRI, no change in the bladders was observed on day 7 after inoculation, filling defect in the bladders, accordant to actual tumor size, was detected on days 14, 21 and 28. Pathologic examination showed that tumor grew in the mucosa or superficial muscle of bladder on day 7, confined in muscle layer on days 14–28, and invaded serosa on day 35. Conclusion: Transurethrally damaged bladder mucosa under direct vision and instilled bladder cancer cell T24, we successfully established an orthotopic bladder cancer model. Tumor growth simulated the progression of human bladder cancer approximately. MRI was a reliable way for dynamic detection of murine orthotopic bladder tumor.

A simple and versatile protocol for isolation of RNA from plant, fungi and animal

Compared to other plants, the traditional protocols for RNA isolation from sugarcane （Saccharum） are usually cJeficient and result in poor yields, or low-quality. Here we describe a modified method for isolating RNA from various fissoes of S. officinarum, in which only Trizol and some routine reagents were needed. The absorbance at 260/280 nm ranges from 1.70 to 1.80, indicating that the quality of RNA is high, as confirmed by double-stranded cDNA synthesis and reverse ~ranscription-polymerase chain （RT-PCR）. This protocol can also be used effectively in RNA isolation from Erianthus arundinaceus, rice （Oryza sativa Linnaeus）, Achyranthes, sporotrichum spp., Thermophil and loach （Misgurnus an g uillicaudatus ）.