动物基因研究的最新进展

科学家最近在小鼠身上进行的初步研究显示:母体基因在后代大脑智力发展方面起主要作用,而父体基因则主要影响大脑中负责情绪的功能。假如这项惊人的发现同时也适用于人类,那么恐怕所有遗传方面的教科书都得重新改写,而且在广泛流行的郎才女貌的说法后面也得画上一个句号了。科学家早就知道基因是成双成对的,其中一个来自母体,一个来自父体。在大多数情况下,每对当中的两个基因看起来都一模一样,都是活跃的。但是有一种基因的情况特殊,这种基因称为印记基因。印记基因携带一个生物化学标记,这个标记不但说明基因来自母体还是父体,而且还决定这个基因在后代的细胞中是否保持活跃状态,科学家发现来自母体和父体的印记基因的活动不一样,由于某种原因印记基因在经过卵子或者精子的时候会进入休眠状态,到最后印记基因结合的时候只有一个保持活跃。科学家不大清楚造成基因休眠的原因。但是猜测(?)

动物基因工程研究室

动物基因工程研究室是陈永福教授组建并领导的研究室,是中国农业大学农业生物技术国家重点实验室的成员研究室之一.本研究室现有教授1人,副教授4人,讲师5人及具有中级职称的技术人员2人.研究生是研究的主力,本研究室在正常情况下有博士和硕士生30位,担纲第一线工作.研究室具备较完善的实验设备,可以进行基因工程、细胞工程和胚胎工程等方面的实验室研究;有可以饲养1000只兔子和500只绵羊的实验动物饲养场,能够进行转基因动物、克隆动物和基因精确修饰动物的研究和生产.

Construction and identification of a vector expressing TRAM siRNA in mammalian cells

Objective:To construct and identify a vector expressing TRAM siRNA in mammalian cells.Methods:It was constructed that the vector named R-pSUPER-EGFP used to transcribe functional TRAM siRNA. Two of pair 64 nt TRAM gene specific target sequences were inserted into the downstream of the H1 promoter. The recombinants were transformed into E. coli JM109, and finally their veracity was confirmed by double cutting with the enzymes and sequencing. R-pSUPER-EGFP was transfected into RAW264.7 cell by using Lipofectamine TM2000, and the expression of TRAM was detected by Western blotting. Results:Two different recombinant plasmids containing corresponding TRAM gene specific target sequences were constructed, transfected into RAW264.7 cell line successively, which can specifically restrain expression of TRAM protein. Conclusion:The optimizing method in constructing the recombinant vector serves other plasmid-based RNA interference research. Therefore, the recombinant vectors establish the basis for research on the function of TRAM gene.