[目的]探讨血管生成性T淋巴细胞(angiogenic T cells,Tang)对子痫前期大鼠胎盘灌注的影响。[方法]采用密度梯度离心法分离健康孕妇胎盘脐带血的单个核细胞,经磁珠分选(MACS)和流式细胞仪分选Tang,再进行培养,制备细胞悬液。选取30只成...[目的]探讨血管生成性T淋巴细胞(angiogenic T cells,Tang)对子痫前期大鼠胎盘灌注的影响。[方法]采用密度梯度离心法分离健康孕妇胎盘脐带血的单个核细胞,经磁珠分选(MACS)和流式细胞仪分选Tang,再进行培养,制备细胞悬液。选取30只成年健康雌性大鼠,发情期按2:1与雄性大鼠同笼饲养,每日观察阴栓脱落情况,发现阴栓日为妊娠d。30只妊娠大鼠随机分为实验组、对照组和正常组,每组10只。实验组和对照组孕鼠自妊娠dr连续5d腹腔注射左旋精氨酸甲酯(L-NAME)250 mg/(kg·d),分别于妊娠d、d1o、d13、dio、dyg测量大鼠尾动脉收缩压,发现血压明显升高视为子痫前期孕鼠模型建模成功。建模成功后实验组在尾静脉注射Tang,对照组在尾静脉注射等量生理盐水,均连续6 d。正常组未做任何处理。比较三组大鼠血压、尿蛋白浓度、胎盘血管密度、胎盘重量、胎鼠体重等指标。[结果]妊娠di3、dio、dy,实验组和对照组孕鼠血压、尿蛋白浓度均高于正常组;妊娠dno、dg,实验组血压和尿蛋白浓度显著低于对照组,其差异均有统计学意义(P<0.05)。三组胎鼠数、胎盘重量比较差异无统计学意义(P>0.05);实验组和对照组胎鼠出生体重、胎盘新生血管荧光密度均显著低于正常组,但实验组胎鼠出生体重、胎盘新生血管荧光密度显著高于对照组,其差异均有统计学意义(P<0.05)。胎盘HE染色显示:对照组孕鼠胎盘组织细胞排列紊乱,细胞量少,细胞水肿和空泡样改变较多,胎盘血管间膜增厚,可见纤维素样坏死;实验组胎盘组织较对照组明显改善,无明显细胞水肿及空泡,仍有少量纤维素样坏死,新生血管较对照组明显增多。[结论]Tang移植在一定程度上增加子痫前期大鼠胎盘新生血管密度和改善妊娠结局,表明对胎盘灌注有一定的改善作用。展开更多
Objective: To establish a model for pregnancy-induced hypertension syndrome in rats. Methods: Adult female Wistar rats were randomized into non-pregnant control (NN), non-pregnant cold-stress control (NC), pregnant co...Objective: To establish a model for pregnancy-induced hypertension syndrome in rats. Methods: Adult female Wistar rats were randomized into non-pregnant control (NN), non-pregnant cold-stress control (NC), pregnant control (PN) and pregnant cold-stress (PC) groups. The rats of NN and PN groups were put under 25 ℃ and those of NC and PC groups under (4±2)℃ for 4 h every morning respectively in the whole experimental period. The blood pressure, urine protein, body weight, haematocrit, weight of the placenta and weight and length of the fetus were recorded and the histological changes of the placenta and the kidneys were also studied. Results: The blood pressure and urine protein of the rats of the NC and PC groups after 2 weeks of cold-stress were more significantly increased than the rats of the NN and PN groups. In addition, the weight of the placenta and the weight and length of the fetus were more significantly lower in the former than the latter. Obvious changes of anoxia and ischemia were observed in the tissues of the kidneys and every layer of the placenta. Conclusion: Our findings of hypertension syndrome induced with repeated cold-stress in pregnant rats can be applied to illustrate the pathogenesis of pregnancy-induced hypertension syndrome in human beings.展开更多
文摘[目的]探讨血管生成性T淋巴细胞(angiogenic T cells,Tang)对子痫前期大鼠胎盘灌注的影响。[方法]采用密度梯度离心法分离健康孕妇胎盘脐带血的单个核细胞,经磁珠分选(MACS)和流式细胞仪分选Tang,再进行培养,制备细胞悬液。选取30只成年健康雌性大鼠,发情期按2:1与雄性大鼠同笼饲养,每日观察阴栓脱落情况,发现阴栓日为妊娠d。30只妊娠大鼠随机分为实验组、对照组和正常组,每组10只。实验组和对照组孕鼠自妊娠dr连续5d腹腔注射左旋精氨酸甲酯(L-NAME)250 mg/(kg·d),分别于妊娠d、d1o、d13、dio、dyg测量大鼠尾动脉收缩压,发现血压明显升高视为子痫前期孕鼠模型建模成功。建模成功后实验组在尾静脉注射Tang,对照组在尾静脉注射等量生理盐水,均连续6 d。正常组未做任何处理。比较三组大鼠血压、尿蛋白浓度、胎盘血管密度、胎盘重量、胎鼠体重等指标。[结果]妊娠di3、dio、dy,实验组和对照组孕鼠血压、尿蛋白浓度均高于正常组;妊娠dno、dg,实验组血压和尿蛋白浓度显著低于对照组,其差异均有统计学意义(P<0.05)。三组胎鼠数、胎盘重量比较差异无统计学意义(P>0.05);实验组和对照组胎鼠出生体重、胎盘新生血管荧光密度均显著低于正常组,但实验组胎鼠出生体重、胎盘新生血管荧光密度显著高于对照组,其差异均有统计学意义(P<0.05)。胎盘HE染色显示:对照组孕鼠胎盘组织细胞排列紊乱,细胞量少,细胞水肿和空泡样改变较多,胎盘血管间膜增厚,可见纤维素样坏死;实验组胎盘组织较对照组明显改善,无明显细胞水肿及空泡,仍有少量纤维素样坏死,新生血管较对照组明显增多。[结论]Tang移植在一定程度上增加子痫前期大鼠胎盘新生血管密度和改善妊娠结局,表明对胎盘灌注有一定的改善作用。
文摘Objective: To establish a model for pregnancy-induced hypertension syndrome in rats. Methods: Adult female Wistar rats were randomized into non-pregnant control (NN), non-pregnant cold-stress control (NC), pregnant control (PN) and pregnant cold-stress (PC) groups. The rats of NN and PN groups were put under 25 ℃ and those of NC and PC groups under (4±2)℃ for 4 h every morning respectively in the whole experimental period. The blood pressure, urine protein, body weight, haematocrit, weight of the placenta and weight and length of the fetus were recorded and the histological changes of the placenta and the kidneys were also studied. Results: The blood pressure and urine protein of the rats of the NC and PC groups after 2 weeks of cold-stress were more significantly increased than the rats of the NN and PN groups. In addition, the weight of the placenta and the weight and length of the fetus were more significantly lower in the former than the latter. Obvious changes of anoxia and ischemia were observed in the tissues of the kidneys and every layer of the placenta. Conclusion: Our findings of hypertension syndrome induced with repeated cold-stress in pregnant rats can be applied to illustrate the pathogenesis of pregnancy-induced hypertension syndrome in human beings.
