竹类病虫害动物害种类及其防治

列出竹类病害10种,虫害19种以及动物害2种。介绍了病虫害的为害特征、时间以及防治方法。

野生动物害生态补偿机制建立的关键措施

正确认识和积极解决野生动物害是"天保"工程二期建设进程中一个严肃的问题。建立和健全生态补偿机制是解决问题的最佳途径。基于野生动物害的定义、典型事件的影响及存在问题的分析,提出了野生动物害生态补偿机制建立的关键措施。

基于区块链的病死害动物无害化处理模型

病死害动物随意丢弃和流入市场会严重影响环境卫生与食物安全,传统基于射频识别(radio frequency identification,简称RFID)技术溯源流程构成的中心化监控网络,存在诸多挑战。将区块链技术引入病死害动物的监控网络,有效提升了监控效力与群众信任度,主要体现在:(1)采用去中心化结构,克服了传统监管流程存在的弊端,确保流程可信度;(2)通过智能合约技术最小化恶意或意外事件发生的可能性,降低合约欺诈所造成的损失;(3)采用基于对等网络的分布式账本数据库,保证系统安全稳定。将区块链技术用于病死害动物监控,能极大地提升监控效果、政府公信力、群众安全感,避免农户及非法处理机构不自觉所导致的安全隐患。

Study on the Comparison of Arthropod Communities in Three Kinds of Cruciferous Vegetable Fields at High Mountainous Area

[Objective]This research aimed to compare arthropod communities in different cruciferous vegetable fields at high mountainous area and provide guidance for pest control. [Method] The main arthropod species in 3 kinds of erueiferous vegetable fields at high mountainous area were investigated, the composition of arthropod community in different vegetable fields were analyzed. [Result] The main arthropod species in erueiferous vegetables fields at high mountainous area were similar, but had different quantity compositions. The richness was the highest in radish field. [Conclusion] Crop species had great influence on biological community; we should focus on the main pests in the process of pest control.

野兽中的凶神

野兽中的凶神你说，老虎、狮子和金钱豹谁更厉害、更凶猛些？“老虎厉害！”“狮子更凶猛！”不错，老虎、狮子都是猛兽，性情都特别凶狠，不但弱小的动物害怕被它吃掉，就是经验丰富的老猎手，也惧怕它三分呢！然而更让人畏惧的猛兽却是金钱豹！金钱豹身体矫健，行动敏捷...

Differences in neuroplasticity after spinal cord injury in varying animal models and humans

Rats have been the primary model to study the process and underlying mechanisms of recovery after spinal cord injury. Two weeks after a severe spinal cord contusion, rats can regain weight-bearing abilities without therapeutic interventions, as assessed by the Basso, Beattie and Bresnahan locomotor scale. However, many human patients suffer from permanent loss of motor function following spinal cord injury. While rats are the most understood animal model, major differences in sensorimotor pathways between quadrupeds and bipeds need to be considered. Understanding the major differences between the sensorimotor pathways of rats, non-human primates, and humans is a start to improving targets for treatments of human spinal cord injury. This review will discuss the neuroplasticity of the brain and spinal cord after spinal cord injury in rats, non-human primates, and humans. A brief overview of emerging interventions to induce plasticity in humans with spinal cord injury will also be discussed.

Anti-Helicobacter pylori therapy significantly reduces Helicobacter py/ori-Induced gastric mucosal damage in Mongolian gerbils

AIM: To investigate the effectiveness of 4 d' anti-Helicobacter pylori therapy on the H pylori-infected Mongolian gerbils based on physiological and pathological changes. METHODS: We used 6-wk-old male gerbils orally inoculated with H pylori (ATCC43504, 2×108 CFU/mL). Seven weeks after H pylori inoculation, the animals of study group received 4 d' anti-H pylori triple therapy (H pylori-eradicated group). Seven days later, all animals of the H pylori-eradicated and control groups (H pylori-infected & H pylori-uninfected groups) were sacrificed. We examined gastric mucosal lesions macroscopically, studied gastritis microscopically and determined the stomach weight ratio, myeloperoxidase (MPO) activity and prostaglandin (PG) E2 level. RESULTS: The results showed that both macroscopic and histological gastric damages were significantly less in H pylori-eradicated group than H pylori-infected group. Stomach weight ratio, MPO activity and PGE2 levels were significantly higher in H pylori-infected group than those in the other two groups. CONCLUSION: Four days' anti-H pylori therapy was effective in the improvement of H pylori-induced gastric lesions in Mongolian gerbils.

Peripheral nerve regeneration and intraneural revascularization

Peripheral nerves are particularly vulnerable to injuries and are involved in numerous pathologies for which specific treatments are lacking. This review summarizes the pathophysiological features of the most common traumatic nerve injury in humans and the different animal models used in nerve regeneration studies. ~Ihe current knowledge concerning Wallerian degeneration and nerve regrowth is then described. Finally, the involvement of intraneural vascularization in these processes is addressed. As intraneural vascularization has been poorly studied, histological experiments were carried out from rat sciatic nerves damaged by a glycerol injection. The results, taken together with the data from literature, suggest that revascularization plays an important role in peripheral nerve regeneration and must therefore be studied more carefully.

Review of Factors Affecting the Yield and Quality of Corn Silage

Corn silage is the most popular and important source of energy and fber for ruminants. Much research focused on the chopping length, additives, sealing system, harmful bacteria and their toxins, and so forth, has been done on corn silage both at home in China and abroad. Some suggestions were presented in this review： （1） Corn silage modulation： Whole-plant corn harvested at the milk-ripe stage with a dry matter （DM） content of 30%~40% was selected as silage material and treated by rubbing and cutting. In the process of silage, commercial Lactobacillus buchneri was inoculated, exogenous protease added appropriately, cellulose enzyme added depending on the phenological period, the silage container and the species of the animals, and coextruded polyethylene？polyamide oxygen barrier flm （OB） selected as the seal material. （2） Strengthening management： Aerobic exposure should be avoided at the storage stage and reduced during the feeding phase. The proportion of corn silage was increased depending on the type of animal and their management. （3） Suggestions for further research： Further studies would be needed for the effects of liquid and solid wastes on soil, water and vegetation, and the treatment of these wastes.

害堤动物危害的预防与处理

文章分析了害堤动物的生活习性及分布特点,进而提出预防与处理措施。

Effects of Different Agroecosystems on Prevalence of Different Species of Pests and Coccinellid Predators

This study was aimed at determining the effects of mixed and single cropping agroecosystems on the prevalence of different species of insect pests and coccinellids. It involved six growing crops： maize Zea mays L., beans Phaseolus vulgaris L. and cowpeas Vignaunguiculata L. Walp in single and mixed stands and sampling throughout the phenology of the crops. Eight insect pest species were recorded on maize grown alone, while thirty seven insect species were endemic on cowpea mono cropped and were of six orders whereas twenty two insect pest species occurred on beans. The predator population was most abundant in the mixed stands of maize and beans （2.33 predators per 30 aphids） as compared to their occurrence in pure stands of cowpeas （0.85 predators per 30 aphids） as there were numerous aphids on beans at pre-flowering phase that supported a higher population level of coccinellids. Also, predator population was at the peak during the tussling stage of maize as they fed on the pollen grains while aphids on cowpeas co-existed mutualistically with black ants that protected them against predation. The genus Cheilomenes spp. was the most ubiquitous predator with a mean of 4.00 individuals per 30 aphids while Hippodamiavariegata was the least abundant predator species with a mean of 0.92 individuals per 30 aphids in all the agroecosystems as the Cheilomenes spp. had a faster discovery rate, range of perception, effective capture efficiency and a shorter handling time of a prey.

Effect of naked eukaryotic expression plasmid encoding rat augmenter of liver regeneration on acute hepatic injury and hepatic failure in rats

AIM: To study the protective effect of eukaryotic expression plasmid encoding augmenter of liver regeneration (ALR) on acute hepatic injury and hepatic failure in rats. METHODS: The PCR-amplified ALR gene was recombined with pcDNA3 plasmid, and used to treat rats with acute hepatic injury. The rats with acute hepatic injury induced by intraperitoneal injection of 2 mL/kg 50% carbon tetrachloride (CCl4) were randomly divided into saline control group and recombinant pcDNA3-ALR plasmid treatment groups. Recombinant pcDNA3-ALR plasmid DNA (50 or 200 μg/kg) was injected into the rats with acute hepatic injury intravenously, intraperitoneally, or intravenously and intraperitoneally in combination 4 h after CCl4 administration, respectively. The recombinant plasmid was injected once per 12 h into all treatment groups four times, and the rats were decapitated 12 h after the last injection. Hepatic histopathological alterations were observed after HE staining, the expression of proliferating cell nuclear antigen (PCNA) in liver tissue was detected by immunohistochemical staining, and the level of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) was determined by biochemical method. The recombinant plasmid DNA (200 μg/kg) and saline were intraperitoneally injected into the rats with acute hepatic failure induced by intraperitoneal injection of 4 mL/kg 50% CCl4 after 4 h of CCl4 administration, respectively. Rats living over 96 h were considered as survivals.RESULTS: The sequence of ALR cDNA of recombinant pcDNA3-ALR plasmid was accordant with the reported sequence of rat ALR cDNA. After the rats with acute hepatic injury were treated with recombinant pcDNA3-ALR plasmid, the degree of liver histopathological injury markedly decreased. The pathologic liver tissues, in which hepatic degeneration and necrosis of a small amount of hepatocytes and a large amount of infiltrating inflammatory cells were observed, and they became basically normal in the most effective group after four times of injection of recombinant pcDNA3-ALR plasmid. The indexes of PCNA significantly increased in the recombinant pcDNA3-ALR plasmid treatment groups compared to model group. The level of serum AST and ALT remarkably reduced in recombinant pcDNA3-ALR plasmid treatment groups compared to model group. The results showed that the effect of 200 μg/kg recombinant pcDNA3-ALR plasmid in the rats with acute liver injury was stronger than that of 50μg/kg pcDNA3-ALR DNA.The effect of intravenous injection of recombinant pcDNA3ALR plasmid was better. After the rats with acute hepatic failure were treated with recombinant pcDNA3-ALR plasmid,the survival rate (40%) significantly increased in treatment groups compared to control group (15%, P＜0.01).CONCLUSION: The ALR gene may play an important role in relieving acute hepatic injury and hepatic failure by promoting hepatic cell proliferation and reducing level ofAST and ALT in CCl4-intoxicated rats.

BENEFICIAL EFFECT OF ANACTH－（4－9） ANALOG （ORG2766） IN THE TREATMENT OF EXPERIMENTAL CORTEX LESION IN RATS

This paper reports that lesions of the medial frontal cortex (MFC) caused behavioral deficit in rats and that this deficit could be attenuated by a well formulated treatment with Org2766, an analog of ACTH- (4-9). Wistar rats were distributed in 3 groups: MFC lesion treated with saline (M-N); MFC lesion treated with Org2766 (MO);and sham-operation treated with saline (S). Repeated Org2766 or saline injection commenced from the day of surgery and lasted for 1 3 days. After surgery. the rats were trained in a passive avoidance task and then in an active avoidance task. MFC lesions were found to be strongly associated with behavioral deficits. The M-N group rats displayed poor retention of the passive avoidance response and showed much slower learning of the active avoidance task as compared to S group rats. The result showed that chronic Org2766 administration improved the behavioral performance of both tasks in MFC lesioned rats. The also revealed that the superoxide dismutase (SOD)activity was significantly increased in the M-O group as compared to the M-N group 15 days after surgery. The possible mechanisms related to the beneficial effect of Org2766 on cortex damage are discussed.

Comparing the Impact of a Neonicotinoid and Biorational Agroneem on Herbivorous and Beneficial Arthropods on Cowpea and Tomato

Cowpea （Vigna unguiculata L. Walp.） and tomato （Lycopersicum esculentum L. Karst） are attacked by a wide range of insect pests worldwide. In many cases, pesticides are used to control the pests. In 2010 and 2011, we conducted field experiments to evaluate and compare the effectiveness of a biorational （Agroneem~） and conventional pesticide （imidacloprid or thiamethoxam） on insect pests of these crops. In the first year, two varieties of cowpea （Mississippi Silver and Pinkeye Purple Hull） and two of tomato （Mariana and German Johnson） were used; in the second year, the better performing of each group （Mississippi Silver and Mariana） were used for the study. A split-plot design with four replications in Year 1, and a randomized complete block design with six treatment combinations in Year 2. In the first year, both pesticide groups were applied following manufacturer＇s recommendation （10-14 d cycles）; and in the second year, the application of the pesticides was driven by economic thresholds level （ETL） of insect pests. The most prevalent species of insects recorded in both crops were in the families： Chrysomelidae, Pentatomidae, Cicadellidae, Vespidae, Sarcophagidae, Thripidae and Sphingidae. In both years, the diversity of insects on cowpea was greater than on tomato and more insects were observed in the second year compared to the first, despite the absence of significant difference （P 〉 0.05） between varieties in the treated plots. The yield of cowpea and tomato was comparable in all sprayed plots.

STUDY ON NUDE MICE INOCULATED WITH MYCOBACTERIUM LEPRAE BY MULTIPLE ROUTES

Immune-deficient nude mice were inoculated with nude mouse derived Mycobacteriurn leprae by multiple routes (intravenously. subcutaneously at the foot pads and ears). The results showed that these inoculated animals were capable of producing a great number of Mycobacterium leprae to a level 10(11-12) per gram of tissue. and were detected histopathologically to have heavy leproniatous lesions. The dissemination of the infection was found particularly in sites with lower body temperature.The organisms have a partiality to striated muscles and peripheral nerves. The authors suggest that experimental leprosy in nude mice is a very useful tool for leprosy resarch. especially in countries without armadillos. Compared with the single-route inoculation reported previously. multiple-route inoculation is more available.

EFFECT OF COXSACKIEVIRUS B3 ON ION CHANNEL CURRENTS IN RAT VENTRICULAR MYOCYTES

To investigate the effects of coxsackievirus B 3(CVB 3) on ion channel currents in rat ventricular myocytes. Methods.Rat hearts were isolated with collagenase to acquire single ventricular myocytes, L type voltage dependent calcium channel(VDCC)current (I Ca ),Na + current (I Na ), outward potassium current (I out ), inwardly rectifying potassium current(I KI ) were recorded using whole cell patch clamp techniques. ResultsCVB 3 infection increased I Ca and I out , while decreased I KI ; but it had no obvious effect on I Na . Conclusion.The effects of CVB 3 on I Ca 、 I out 、 I KI may be one of the mechanisms of myocytes damage and the occurrence of abnormal electroactivities induced by CVB 3 infection.

The Post-exposure Effects of Spinosad and Plant Allelochemicals on Biological Characteristics of Helicoverpa armigera(Lepidoptera： Noctuvidae)

In order to define the combined effects of spinosad and plant allelochemicals on Helicoverpa armigera(Lepidoptera:Noctuvidae), the research fed them on artificial diets with different doses of spinosad, gossypol, quercetin and tannicacid, and the changes of pupal and imaginal biological characteristics were observed and recorded. The results showed that spinosad at sublethal concentrations decreased pupation ratio and pupal weight, prolonged prepupal and pupal periods, and decreased emergence ratio, fecundity and longevity of adults. Combined with plant allelochemicals, spinosad significantly prolonged pupal period and shorten adult longevity. Other life-cycle parameters such as pupation ratio, pupal weights, emergence ratio, eggs laid per female and hatch ratio did not change significantly. The results clearly indicated that plant allelochemicals did not change spinosad efficiency on H. armigera, but the combination of spinosad and plant allelochemicals might affect pest population dynamics significantly by changing its development.

Patterns,Causes and Perceptions of Human-Large Carnivore Conflict in the Chitwan National Park,Nepal

One of the major management problems in and around protected areas is the intensifying conflict between local people and wildlife,especially large carnivores.Livestock depredation and human fatalities caused by attacks of carnivores are found to be serious obstacles in conflict management.This study aims to explore the patterns,costs,causes and perceptions of human large carnivore conflict in the Nawalpur area of Chitwan National Park.The patterns of livestock loss and human casualties due to large carnivores(Panthera tigris and Panthera pardus)were analysed using the secondary data reported to Chitwan National Park from 2001 to 2019.To understand the people’s perception towards carnivores and wildlife conservation,150 victim respondents were asked,with one household selected from each grid.During 19-year study period,a total of 521 incidents caused by large carnivores were reported,which included 33 human casualties and 488 livestock depredations.Tiger was responsible for the maximum conflict incidents in Nawalpur.The total relief provided for human deaths and injuries was US$17524.41,whereas US$13702.18 was used to compensate for livestock depredation in the Nawalpur area by authorities of the Chitwan National Park.More than 64%of the respondents liked the presence of carnivores in their area and had a positive attitude toward the conservation of large carnivores,even though the carnivores were responsible for livestock depredation and human injury and death.People’s satisfaction with the relief scheme provided by the government depended on ethnicity,gender,age class,occupation,education,insurance,and livestock ownership,but they believed the scheme was not effective.Hence,an effective relief scheme and awareness about the process of relief funding should be conducted in conflict areas.Conducting awareness programs for local communities about large carnivores,their behavior,and preparing predator-proof corrals would be helpful in minimizing conflict in the study area.

A totally laparoscopic peritoneal free flap for reconstruction of hand

Management of defects on the hand and foot with exposed tendons remains a major challenge for plastic surgeons. Here, we present a case of hand reconstruction with a totally laparoscopic peritoneal flap. The anterior rectus sheath was preserved in situ. The peritoneal free flap supplied by peritoneal branches of the deep inferior epigastric artery was retrieved by laparoscopy to cover the soft tissue defect of the hand. The defect of the dorsal hand was 17 cm × 12 cm. The peritoneal flap measuring 22 cm × 15 cm survived completely without any complications. A following split-thickness skin graft offered the suc- cessful wound closure. Motor and sensory function improved gradually within the first year follow-up. The totally laparoscopic peritoneal free flap is a good choice for reconstruction of the soft tissue de- fects accompanied by exposed tendons on the hand and foot.