密闭式鸡舍动物水平高度和人体水平高度环境状况对比

畜舍环境控制系统对畜禽的健康和生产有着重要的影响,一是可以使动物周围的空气维持合适的温度、湿度和风速,避免热应激和冷应激的出现。二是排出畜舍中的有害气体、灰尘和微生物,改善畜禽舍的空气环境质量。在畜禽环境设计、运行和环境管理中,一般都以畜舍内的空气环境为目标,而很少以动物真正感觉到的环境状况,即动物周围的环境情况为判定依据。为此,作者在北京某鸡场密闭式育雏育成平养鸡舍中进行了试验研究,分别在加热期间、离温初期和育雏育成末期三个阶段,对0.2m的动物水平和1.5m人体水平的环境参数进行了现场测试、分析和比较。

提高动物检疫工作水平的方法

动物检疫工作是肩负着预防动物疫病职能的重要畜牧兽医工作,目前畜牧兽医工作中动物检疫工作水平仍有提升的空间。文章阐述了畜牧兽医工作与动物检疫工作,对动物检疫工作存在的问题进行了分析,提出了提升动物检疫工作水平的方法。

进一步提升宣威市动物防疫工作水平的对策及建议

宣威市动物防疫面临国际国内重大动物疫情的严峻威胁。文章从财政保障、取消检疫收费、调整扑杀动物和销毁动物产品补偿政策,争取兽医实验室建设和加大动物防疫监督执法力度等几方面,提出进一步提升宣威动物防疫水平的建议。

MAPREC与MNVT评价Ⅲ型Sabin株脊髓灰质炎病毒神经毒力的比较

目的用PCR扩增及限制性酶切分析突变技术(MAPREC)和猴体神经毒力试验(MNVT)评价同一批Ⅲ型Sabin株脊灰病毒神经毒力的结果差异。方法采用MAPREC技术,检测Ⅲ型Sabin株脊灰病毒神经毒力关键位点472位点突变率;采用MNVT病理切片分析,评价Ⅲ型Sabin株脊灰病毒神经毒力。结果 MAPREC检测待测样品核酸472位点突变率均值为0.324%,低于高突变参考品的0.621%。MNVT切片结果分析表明,xtest–xref=0.169,小于C1。对同一批样品,MAPREC与MNVT分析结果一致。结论在Ⅲ型Sabin株脊灰病毒神经毒力检测方面,操作简单,试验周期短的MAPREC技术可作为金标准MNVT的有益补充。

Concentrations and distribution of organochlorine pesticides in shellfish from Changjiang estuary

To analyze and evaluate the status of organochlorine pollutants in the Changjiang （Yangtze River） estuary and adjacent waters, the concentrations of hexachlorocyclohexane （HCHs） and dichlorodiphenyltrichloroethane （DDTs） in shellfish collected in study area from 2006 to 2007 were determined with gas chromatography （GC）. The concentration range of HCHs was （ND-12.13）×10^-3 mg/kg wet weight and averaged at 0.54×10^-3 mg/kg while the concentration of DDTs was in the range of （4.06-281.73） ×10^-3 mg/kg with a mean of 57.52×10^-3 mg/kg in the survey areas. The concentrations of DDTs in the shellfish were higher than HCHs＇, so that DDTs could be considered as typical organochlorine pollutants in the areas. The concentrations of DDTs in the shellfish were higher than HCHs＇, so that DDTs could be considered as typical organochlorines pollutants. The HCHs in all the shellfish conformed to the first level of criterion （0.02 mg/kg） of the Marion Biology Quality （GB 18421-2001）, and that of DDTs in most samples were beyond the first level （0.01 mg/kg） but conformed to the second level （0.10 mg/kg）. On average, α-HCH and δ-HCH occupied the most part of HCHs, while O,P＇-DDT and P,P＇-DDT occupied the most part of DDTs. The concentrations of organocholorine pesticides in shellfish samples varied in site and in species. The highest level occurred at the Shengsi （SS）, followed by Yangkougang （YKG）, Lvsi （LS）, Dongyuan （DY） and Beibayao （BBY）, low concentrations were observed at Changsha （CS）, Beidaodi （BDD）, and Gouqi （GQ）. The concentration of HCHs and DDTs in most sites decreased clearly from 2006 to 2007 except for YKG, DY, BDD, LYS, and SS. All of above results suggested that the study area was slightly affected by organochlorine pesticide, special by DDTs.

Prunella vulgaris L. extract improves cellular immunity in MDR-TB challenged rats

Objective： To study the effect of the extract of Prunella vulgaris L. on multiple drugs resistant bacillus tuberculosis （MDR-TB）. Methods： Experimental animal model in rats was induced by MDR-TB. Normal group model group and Prunella vulgaris L. group were set up. The contents of IFN-7, IL-4, IL-10 and IL-12 were examined by ELISA. Their genome mRNAs were extracted, the target genes were amplified by PCR. RT-PCR was used to detect the mRNA levels of them. Results： The content of IFN-q, of the extract of Prunella vulgaris L. group was 1.98±0.67 pg/ml, IL-4 was 6.47±1.46 pg/ml, IL-10 was 12.13±3.43 pg/ml and IL-12 was 3.02±0.86 pg/ml. Compared with the model group, Prunella vulgaris L. group was notable difference in serum IFN-γ, IL-12 and IL-10 （P〈0.05）. The mRNA levels of IFN-γ, IL-12 increased and IL-10 decreased obviously, the differences were quite significant （P〈0.05）, but IL-4 had no obvious change. Conclusion： The extract of Prunella vulgaris L. can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription, accordingly provide the theory basis of healing of tuberculosis with it.

Emerging role of microRNAs in liver diseases

MicroRNAs are a class of small non-coding RNAs that are found in plants, animals, and some viruses. They modulate the gene function at the post-transcriptional level and act as a fine tuner of various processes, such as development, proliferation, cell signaling, and apopto-sis. They are associated with different types and stages of cancer. Recent studies have shown the involvement of microRNAs in liver diseases caused by various factors, such as Hepatitis C, Hepatitis B, metabolic disorders, and by drug abuse. This review highlights the role of microRNAs in liver diseases and their potential use as therapeutic molecules.

Effect of approach run velocity on the optimal performance of the triple jump

Purpose： The purpose of this study was to determine the effect of horizontal and vertical velocities at the landing of the last step of approach run on the performance and optimal phase ratio of the triple jump. Methods： Three-dimensional kinematic data of 13 elite male triple jumpers were obtained during a competition. Computer simulations were performed using a biomechanical model of the triple jump to determine the longest actual distance using the optimal phase ratio with altered horizontal and vertical velocities at the landing of the last step of approach run. Results： The actual distance obtained using the optimal phase ratio significantly increased as the horizontal velocity at the landing of the last step of approach run increased （p = 0.001） and the corresponding downward vertical velocity decreased （p = 0.001）. Increasing horizontal velocity at the landing of the last step of approach run decreased optimal hop percentage and increased optimal jump percentage （p = 0.001）, while decreasing corresponding downward vertical velocity increased optimal hop percentage and decreased optimal jump percentage （p = 0.001）. Conclusion： The effects of the velocities at the landing of the last step of approach run on the optimal phase ratio were generally small and did not qualitatively alter optimal techniques.

农业部印发意见推进大东北无疫区建设

农业部日前印发《关于推进大东北地区免疫无口蹄疫区建设的指导意见》，对接国家东北振兴战略，深入贯彻落实《国家中长期动物疫病防治规划（2012—2020年）》，推进犬东北地区免疫无口蹄疫区（以下简称大东北无疫区）建设，指导地方提升区域动物卫生水平，助力大东北地区打造菜篮子供给基地、

Comparison of daily physical activity parameters using objective methods between overweight and normal-weight children

Purpose: The purpose of the present study was to determine if there were any differences in various aspects of physical activity such as energy expenditure, intensity, and type of activity between normal-weight and overweight boys.Methods: Children aged 9—12 years were recruited from 2 elementary schools located in different urban districts in Republic of Korea. The present study included 45 Korean boys, of which 19 were normal-weight and 26 were overweight. Daily physical activity was estimated over the same 1-week study period under free-living conditions using the doubly labeled water(DLW) method and a tri-axial accelerometer. Resting metabolic rate(RMR) was measured using the Douglas bag method and open-circuit indirect calorimetry. We calculated the physical activity level(PAL) as the total energy expenditure(TEE)/RMR.Results: PAL was not significantly different between the groups. In the accelerometer data, time spent in locomotive moderate-to-vigorous physical activity(MVPA) was significantly lower in overweight boys than in normal-weight subjects, whereas other variables including non-locomotive activity did not differ between groups. In addition, among all participants, time spent in total locomotive activity was significantly associated with PAL. Time spent in locomotive MVPA was significantly associated with PAL.Conclusion: Overweight boys may be less physically active based on locomotive MVPA, which was positively related with PAL. Our findings suggest that the contribution of locomotive MVPA to the increase in PAL was relatively significant.

Motion Geometric Active Contours: Tracking Nonrigid Objects in Clutter Background

MGAC (Motion Geometric Active Contours), a new variational framework of geometric active contours to track multiple nonrigid moving objects in the clutter background in image sequences is presented. This framework, incorporating with the motion edge information, consists of motion detection and tracking stages. At the motion detection stage, the motion edge map provides an approximate edge map of the moving objects. Then, a tracking stage, merely using the static edge information, is considered to improve the motion detection result. Force field regularization method is used to extend the capture range of the edge attraction force field in both stages. Experiments demonstrate that the proposed framework is valid for tracking multiple nonrigid objects in the clutter background.

Impact of Industrial Pollution on the Zooplankton Population Diversity of the Hammam Boughrara Dam

Works concerning zooplankton of freshwaters of the western area of Algeria are only very fragmentary and remain at an embryonic state. Remediating to this situation, it led a study of the biodiversity and zooplanktonic populations distribution in time and space in the Boughrara dam lake which remains subject to a strong pollution coming essentially from Morocco. The preliminary results of the faunistic analysis of the zooplanktonic populations enabled to highlight an important richness of zoological groups. The CFA （correspondences factorial analysis） and the AHC （ascending hierarchical classification） methods used both enabled to define the species distribution of both open waters and littoral ones, and also to perform existing affinities between them and the state of the used medium. Compared to other barrages, such as those of Bouhanifia and of Sarno, the zooplanktonic populations of both open waters and littoral populations appear to a great extent numerous in rain period. On the quantitative and qualitative level, the zooplanktonic populations strongly suffer from the pollution contamination coming from the Mouillah River especially in summer period. It is time to think about the protection of this fauna which is the second trophic chain link and this intending to preserve the ecosystems in an equilibrium state and to consider the exploitation of natural resources in the realization of an aquacultural farm.

Bioaccumulation of Mercury in Fish Species from Different Trophic Level

Mercury （Hg） is an environmental pollutant, and can bioaccumulate and biomagnify in the food web. Twenty four fish samples of different tropic level i.e., Herbivore, Omnivore and Carnivore were collected from two contaminated rivers, the Megna and the Buriganga which surround the Dhaka division for the assessment of toxic Hg. Fourteen samples were found to contain Hg in the range of 0.01-0.09 mg/kg. The highest Hg （0.09 mg/kg） was found in the Carnivore, Bele （Glossogobius giuris）. However, all fish species had lower amount of Hg than the maximum Hg limit （0.5 mg/kg for fish） set by World Health Organization （WHO）. Among all the fish species, the order of bioaccumulation was carnivore 〉 omnivore 〉 herbivore. Kajoli （Ailia coila）, Shing （Heteropnuestes fossilis）, Rui （Labeo rohita）, Chewa （Tryauchen vagine）, Rita （Rita rita）, Hilsha （Tenualosa ilisha）, Small Puti （Puntius sophore）, Bacha （Eutropiichthys vacha） and Chingri （smallprawn） were not found to contain any Hg which indicated that fish species from these rivers are safe for human consumption.

Dynamics of Lumber Production from Buttressed-Stumps of Logging Residues Using a Fuel Powered Horizontal Mobile Bandsaw Machine

Logging residue can be defined as any form of wood, which under the highest stage of technological development could be used in manufacturing but is left in the forest during logging. Lumber production from logging residues of a previous logging activity by a timber firm was undertaken with the objective of determining the suitability of utilizing buttressed-stumps as raw material for the timber industry. A horizontal mobile bandsaw machine was used to process the buttressed-stumps into lumber. The machine was characterized by a thin-kerr sawing technology （kerf-width 1.6 mm） compared to the conventional bandsaw machines of kerr-widths ranging from 3.0-4.5 mm. Lumber value and volume yields, fuel consumption rate, frequency of tool replacement and lumber production rate were assessed. Results indicated that there is the potential to increase timber production from logging residues by utilizing buttressed-stumps. Lumber value and volume yields of eight timber species investigated in this study ranged from 5%-31% and 34%-54% respectively. Fuel consumption rate which increased with increasing wood density, ranged from 5-14.5 liters/m3 of lumber produced. Frequency of saw replacement increased with increasing wood density. The number of saws required to produce one cubic meter of lumber, ranged from 1 to 7. Lumber production rate ranged from 0.10-0.38 m3/hour, increasing with decreasing wood density.

Rare horizontal transmission does not hide long-term inheritance of SINE highly conserved domains in the metazoan evolution

Transposable elements （TEs） are self-replicating, mobile DNA sequences which constitute a significant fraction of eukaryotic genomes. They are generally considered selfish DNA, as their replication and random insertion may have deleterious effects on genome functionalities, although some beneficial effects and evolutionary potential have been recognized. Short interspersed elements （SINEs） are non-autonomous TEs with a modular structure： a small RNA-related head, a body, and a long interspersed element-related tail. Despite their high turnover rate and de novo emergence, the body may retain highly conserved domains （HCDs） shared among divergent SINE families： in metazoans, at least nine HCD-SINEs have been recognized. Data mining on public molecular databases allowed the retrieval of 16 new HCD-SINE families from cnidarian, molluscs, arthropods, and vertebrates. Tracking the ancestry of HCDs on the metazoan phylogeny revealed that some of them date back to the Radiata-Bilateria split. Moreover, phylogenetic and age versus divergence analyses of the most ancient HCDs suggested that long-term vertical inheritance is the rule, with few horizontal transfer events. We suggest that the evolutionary conservation of HCDs may be linked to their potential to serve as recombination hotspots. This indirectly affects host genomes by maintaining active and diverse SINE lineages, whose insertions may impact （either positively or negatively） on the evolution of the genome.