为深入了解国内外动物源活性肽的研究现状和热点,基于中国知网(CNKI)和Web of Science核心合集(SCI-E)数据库,采用文献计量学理论和可视化分析技术,对2016~2020年期间发表的研究文献进行分析。通过收集的1005篇中文文献和2063篇英文文献...为深入了解国内外动物源活性肽的研究现状和热点,基于中国知网(CNKI)和Web of Science核心合集(SCI-E)数据库,采用文献计量学理论和可视化分析技术,对2016~2020年期间发表的研究文献进行分析。通过收集的1005篇中文文献和2063篇英文文献,从文献时序性、发文国家及合作关系、研究机构、期刊分布和关键词等角度进行知识图谱分析,以期全面展示动物源活性肽的研究现状。结果表明,动物源活性肽的研究呈现快速发展趋势,每年的英文文献发文量在逐渐上升,中文文献保持稳定;全球共有84个国家/地区开展动物源活性肽的研究,中国研究水平处于领先地位,且与美国、德国和印度等国家跨区域合作频繁,俄罗斯科学院和中国科学院为核心研究机构,国内外期刊发文量分别以《食品科学》、《Scientific Reports》最多,抗菌肽作用机制是学者们关注的热点。抗菌肽的免疫调节功能、结构鉴定及其在解决抗生素抗药性方面的应用是该领域的研究趋势。展开更多
AIM: The sphincter of Oddi (SO) plays an important role in delivery of bile into the duodenum. To establish whether vasoactive intestinal polypeptide (VIP) and nitric oxide (NO) were involved in phasic contractile act...AIM: The sphincter of Oddi (SO) plays an important role in delivery of bile into the duodenum. To establish whether vasoactive intestinal polypeptide (VIP) and nitric oxide (NO) were involved in phasic contractile activity of the rabbit SO stimulated by cholecystokinin-octapeptide (CCK-8). METHODS: Isolated SO muscle rings were cleaned of fat and mounted horizontally on two small L-shaped hooks one of which was connected to a force transducer for the measurement of isometric tension.The experiments were carried out in a thermostatically controlled (37±0.2℃) organ bath (5 mL) containing Krebs solution.The organ fluid was gassed with 95% O2 and 50 mL/L CO2 to keep the pH at 7.40±0.05. Contractile responses to CCK-8 (1 μmol/L) were evaluated in the presence and absence of NG-nitro-L-arginine (LNNA), an inhibitor of NO synthase (100 μmol/L), and (p-chloro-D-Phe6-Leu17)-VIP (VlPa, 30 μmol/L), a VIP receptor antagonist. RESULTS: CCK-8 stimulated the phasic activity of the SO. NO synthase inhibition increased the frequency and amplitude of contractions with a slight increase in developed tension. Pre-incubation with VlPa also attenuated this CCK-8 effect. The combined application of LNNA and VlPa abolished the phasic activity of the muscle rings with a marked increase in tension in response to CCK-8. CONCLUSION: VIP and NO together contribute to an increase in phasic activity of SO.展开更多
Angiotensin （Ang）-（1-7） is recognized as a new bioactive peptide in renin-angiotensin system （RAS）. Ang-（1-7） is a counter-regulatory mediator of Ang-II which appears to be protective against cardiovascular di...Angiotensin （Ang）-（1-7） is recognized as a new bioactive peptide in renin-angiotensin system （RAS）. Ang-（1-7） is a counter-regulatory mediator of Ang-II which appears to be protective against cardiovascular disease. Recent studies have found that Ang-（1-7） played an important role in reducing smooth muscle cell proliferation and migration, improving endothelial function and regulating lipid metabolism, leading to inhibition of atherosclerotic lesions and increase of plaque stability. Although clinical application of Ang-（1-7） is restricted due to its pharmacokinetic properties, identification of stabilized compounds, including more stable analogues and specific delivery compounds, has enabled clinical application of Ang-（1-7）. In this review, we discussed recent findings concerning the biological role of Ang-（1-7） and related mechanism during atherosclerosis development. In addition, we highlighted the perspective to develop therapeutic strategies using Ang-（1-7） to treat atherosclerosis.展开更多
文摘为深入了解国内外动物源活性肽的研究现状和热点,基于中国知网(CNKI)和Web of Science核心合集(SCI-E)数据库,采用文献计量学理论和可视化分析技术,对2016~2020年期间发表的研究文献进行分析。通过收集的1005篇中文文献和2063篇英文文献,从文献时序性、发文国家及合作关系、研究机构、期刊分布和关键词等角度进行知识图谱分析,以期全面展示动物源活性肽的研究现状。结果表明,动物源活性肽的研究呈现快速发展趋势,每年的英文文献发文量在逐渐上升,中文文献保持稳定;全球共有84个国家/地区开展动物源活性肽的研究,中国研究水平处于领先地位,且与美国、德国和印度等国家跨区域合作频繁,俄罗斯科学院和中国科学院为核心研究机构,国内外期刊发文量分别以《食品科学》、《Scientific Reports》最多,抗菌肽作用机制是学者们关注的热点。抗菌肽的免疫调节功能、结构鉴定及其在解决抗生素抗药性方面的应用是该领域的研究趋势。
基金Supported by The Wellcome Trust (Grant No. 022618),by the Hungarian Scientific Research Fund (D42188, T43066 and T042589)and by the GVOP-3.2.2.-2004-07-0001/3.0
文摘AIM: The sphincter of Oddi (SO) plays an important role in delivery of bile into the duodenum. To establish whether vasoactive intestinal polypeptide (VIP) and nitric oxide (NO) were involved in phasic contractile activity of the rabbit SO stimulated by cholecystokinin-octapeptide (CCK-8). METHODS: Isolated SO muscle rings were cleaned of fat and mounted horizontally on two small L-shaped hooks one of which was connected to a force transducer for the measurement of isometric tension.The experiments were carried out in a thermostatically controlled (37±0.2℃) organ bath (5 mL) containing Krebs solution.The organ fluid was gassed with 95% O2 and 50 mL/L CO2 to keep the pH at 7.40±0.05. Contractile responses to CCK-8 (1 μmol/L) were evaluated in the presence and absence of NG-nitro-L-arginine (LNNA), an inhibitor of NO synthase (100 μmol/L), and (p-chloro-D-Phe6-Leu17)-VIP (VlPa, 30 μmol/L), a VIP receptor antagonist. RESULTS: CCK-8 stimulated the phasic activity of the SO. NO synthase inhibition increased the frequency and amplitude of contractions with a slight increase in developed tension. Pre-incubation with VlPa also attenuated this CCK-8 effect. The combined application of LNNA and VlPa abolished the phasic activity of the muscle rings with a marked increase in tension in response to CCK-8. CONCLUSION: VIP and NO together contribute to an increase in phasic activity of SO.
基金This work was supported by National Natural Science Foundation of China （NSFC） （No. 81400265 and No. 81270274）, and Peking University People＇s Hospital Research and Development funds （RDB2014-16）.
文摘Angiotensin （Ang）-（1-7） is recognized as a new bioactive peptide in renin-angiotensin system （RAS）. Ang-（1-7） is a counter-regulatory mediator of Ang-II which appears to be protective against cardiovascular disease. Recent studies have found that Ang-（1-7） played an important role in reducing smooth muscle cell proliferation and migration, improving endothelial function and regulating lipid metabolism, leading to inhibition of atherosclerotic lesions and increase of plaque stability. Although clinical application of Ang-（1-7） is restricted due to its pharmacokinetic properties, identification of stabilized compounds, including more stable analogues and specific delivery compounds, has enabled clinical application of Ang-（1-7）. In this review, we discussed recent findings concerning the biological role of Ang-（1-7） and related mechanism during atherosclerosis development. In addition, we highlighted the perspective to develop therapeutic strategies using Ang-（1-7） to treat atherosclerosis.
